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Mechanism of resveratrol on the promotion of induced pluripotent stem cells

Journal of Integrative Medicine 2013年第6期11卷 389-396页

作者： dao-fang ding Xiao-feng Li Hao Xu Zhen Wang Qian-qian Liang Chen-guang Li Yong-jun Wang Institute of Spine Shanghai University of Traditional Chinese Medicine Longhua Hospital Shanghai University of Traditional Chinese Medicine Laboratory of Cancer Hospital Fudan University

OBJECTIVE： To investigate the effects of resveratrol （RV） in reprogramming mouse embryonic fibroblasts （MEFs） into induced pluripotent stem cells （iPSCs） and the related mechanism. METHODS： Primary MEFs were isolated from E13.5 embryos and used within three passages. Retroviruses expressing Sox2 and Oct4 were produced by transfecting GP2-293t cells with recombinant plasmids murine stern cell virus （MSCV）-Sox2 and MSCV-Oct4. Supernatants containing retroviruses were obtained after 48-hour transfection and MEFs were then infected. Different concentrations （0, 5, 10 and 20 IJmol/L） of RV were added to embryonic stem cell （ESC） medium to culture MEFs 48 h post-infection, iPSC clones emerged and were further cultured. Expression of pluripotent markers of iPSCs was identified by cell immunofluorescence and reverse transcription-polymerase chain reaction. Both cytotoxicity and cell proliferation were assayed by Western blot analysis after RV was added into ESC medium. The ultrastructure change of mitochondria was observed by electron microscopy. RESULTS： More than 2.9-fold and 1.3-fold increases in colony number were observed by treatment with RV at 5 and 10 pmol/L, respectively. The reprogramming efficiency was significantly decreased by treatment with 20 pmol/L RV. The proliferation effect on MEFs or MEFs infected by two factors Sox2/Oct4 （2 factors-MEFs, 2F-MEFs） was investigated after RV treatment. At 20 pmol/L RV, induced cell apoptosis and proliferation inhibition were more obvious than those of 5 and 10 IJmol/L treatments. Clones were selected from the 10 pmol/L RV-treated group and cultured. Green fluorescent protein expression from one typical clone was silenced one month later which expressed ESC-associated marker genes Gdf3, Nanog, Ecatl, Fgf4 and Foxd3. Electron transmission microscope showed obvious cavitations in mitochondria. The expression of hypoxia-inducible factor-la was up-regulated when 2F-MEFs were treated with RV compared to the control group. CONCLU

关键词： resveratrol plant extracts pluripotent stem cells hypoxia mitochondria cavitation in vitro

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Protective Effect of Ligustrazine on Lumbar Intervertebral Disc Degeneration of Rats Induced by Prolonged Upright Posture

Protective Effect of Ligustrazine on Lumbar Intervertebral D...

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第十四届全国中药和天然药物学术研讨会

作者： Qian-Qian Liang Yan-Chen dao-fang ding Zhi-Jie Xi Chen-Guarig Li Shu-Fen Liu Sheng Lu Yong-Jian Zhao Qi Shi Yong-Jun Wang Shanghai University of Traditional Chinese Medicine

＜正＞Most chronic low back pain is the result of degeneration of the lumbar intervertebral ***,an alkaloid from Chuanxiong,reportedly is able to relieve pain,suppress inflammation,and treat osteoarthritis;and it has the protective effect on cartilage and ***,we asked whether ligustrazine could reduce intervertebral disc

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蛇床子素对大鼠原代软骨细胞增殖的抑制作用

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中西医结合学报 2012年第12期10卷 1413-1418页

作者：丁道芳韦宋谱李晓锋张晓钢詹红生段铁骊曹月龙上海市中医药研究院骨伤科研究所上海201203 上海中医药大学曙光医院石氏伤科医学中心上海201203 上海中医药大学脊柱病研究所上海200032 上海中医药大学龙华医院上海200032

目的:观察蛇床子素对大鼠软骨细胞增殖的影响。方法:分离出生24h内大鼠的软骨细胞,进行原代培养。细胞扩增至第2代时观察1周内细胞增殖情况,并用细胞免疫荧光法鉴定Ⅱ型前胶原基因(typeⅡprocollagen gene,Col2a1)的表达。将第2代软骨... 详细信息

目的:观察蛇床子素对大鼠软骨细胞增殖的影响。方法:分离出生24h内大鼠的软骨细胞,进行原代培养。细胞扩增至第2代时观察1周内细胞增殖情况,并用细胞免疫荧光法鉴定Ⅱ型前胶原基因(typeⅡprocollagen gene,Col2a1)的表达。将第2代软骨细胞分为对照组和不同浓度蛇床子素(6.25、12.5、25和50μmol/L)组。药物作用24和48h后,观察细胞增殖情况,蛋白质印迹法和聚合酶链反应法检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和细胞周期素D1(cyclinD1)的表达。结果:第2代原代软骨细胞活力正常,Col2a1表达明显。蛇床子素可以抑制软骨细胞的增殖,并随浓度的增加,抑制作用愈明显。蛋白质印迹法和聚合酶链反应法检测发现,随着蛇床子素浓度的增加,软骨细胞中PCNA和cyclinD1的表达下降。结论:蛇床子素可能通过抑制PCNA和cyclinD1的表达发挥抑制软骨细胞增殖的作用。

关键词：植物提取物蛇床子素软骨细胞细胞增殖增殖细胞核抗原细胞周期蛋白D1 大鼠

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Comparison of the Structural Evolution ofβPolypropylene during the Sequential and Simultaneous Biaxial Stretching Process

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Chinese Journal of Polymer Science 2021年第5期39卷 620-631,I0008页

作者： dao-Xin Zhang Lei ding Feng Yang fang Lan Ya Cao Ming Xiang State Key Laboratory of Polymer Materials Engineering Polymer Research Institute of Sichuan UniversityChengdu 610065China

In this work,the lamellar structural evolution and microvoids variations of βpolypropylene(β-PP)during the processing of two different stretching methods,sequential biaxial stretching and simultaneous biaxial stretching,were investigated in *** was found that different stretching methods led to significantly different lamellae deformation modes,and the microporous membranes obtained from the simultaneous biaxial stretching exhibited better mechanical *** the sequential biaxial stretching,abundant coarse fibers originated from the tight accumulation of the lamellae parallel to the longitudinal stretching direction,whereas the lamellae perpendicular to the stretching direction were easily deformed and *** coarse fibers were difficult to be separated to form micropores during the subsequent transverse stretching process,resulting in a poor micropores ***,for the simultaneous biaxial stretching,theβcrystal had the same deformation mode,that is,the lamellae distributed in different directions were all destroyed,forming abundant microvoids and little coarse fibers.

关键词： βPolypropylene Simultaneous biaxial stretching Sequential biaxial stretching Structure evolution

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A solution to parabolic system with the fractional Laplacian

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Applied Mathematics(A Journal of Chinese Universities) 2009年第2期24卷 184-190页

作者： fang Lin fang dao-yuan Dept. of Math. Zhejiang Univ. Hangzhou 310027 China

The existence of a solution to the parabolic system with the fractional Laplacian （-△） α/2, α 〉 0 is proven, this solution decays at different rates along different time sequences going to infinity. As an application, the existence of a solution to the generalized Navier-Stokes equations is proven, which decays at different rates along different time sequences going to infinity. The generalized Navier-Stokes equations are the equations resulting from replacing -△ in the Navier-Stokes equations by （-△）^m, m〉 0. At last, a similar result for 3-D incompressible anisotropic Navier-Stokes system is obtained.

关键词： parabolic system fractional Laplacian decay rate

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Non-formation of vacuum states for Navier-Stokes equations with density-dependent viscosity

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第10期8卷 1681-1690页

作者： ZHANG Ting fang dao-yuan Department of Mathematics Zhejiang University Hangzhou 310027 China

We consider the Cauchy problem, free boundary problem and piston problem for one-dimensional compressible Navier-Stokes equations with density-dependent viscosity. Using the reduction to absurdity method, we prove that the weak solutions to these systems do not exhibit vacuum states, provided that no vacuum states are present initially. The essential re- quirements on the solutions are that the mass and energy of the fluid are locally integrable at each time, and the Lloc1-norm of the velocity gradient is locally integrable in time.

关键词： Compressible Navier-Stokes equations Vacuum states Density-dependent viscosity

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MC-ICP-MS 测定岩石标准物质的钒同位素组成

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岩矿测试 2025年第1期44卷 63-74页

作者：徐丽怡于慧敏丁昕黄方中国科学技术大学地球和空间科学学院岩石圈演化与环境演变全国重点实验室安徽合肥230026 中国科学院比较行星学卓越创新中心中国科学技术大学安徽合肥230026

随着分析方法的发展和分析精度的提升,钒同位素已经越来越多地被用于各种地质过程研究。为了确保钒同位素分析测试过程中,可以有效地监控数据的准确度和精度,方便国际各实验室之间的数据对比,同时考虑到早期国际上常用美国地质调查局(US... 详细信息

随着分析方法的发展和分析精度的提升,钒同位素已经越来越多地被用于各种地质过程研究。为了确保钒同位素分析测试过程中,可以有效地监控数据的准确度和精度,方便国际各实验室之间的数据对比,同时考虑到早期国际上常用美国地质调查局(USGS)的标准物质面临着库存不足等问题,本文采用多接收电感耦合等离子体质谱仪(MC-ICP-MS)测定了一系列国际地质标准物质和国家地质标准物质的钒同位素组成,δ51V值的测试精度优于0.08‰。本文选取的标准物质主要来自日本地质调查局(GSJ)和中国地质科学院地球物理地球化学勘查研究所(IGGE),包括一个安山岩(JA-1),三个玄武岩(JB-3、JB-1b和GBW07105),一个辉长岩(JGb-1),一个辉绿岩(GBW07123)以及一个土壤(GBW07454),钒含量范围为77~635μg/g,涵盖了目前大部分火成岩和部分土壤等天然样品含量的范围。这些标准物质除了土壤GBW07454,其他标准物质的钒同位素组成未曾被报道。经测量表明,辉长岩标准物质JGb-1具有最高δ51V值,为−1.05‰±0.08‰,安山岩标准物质JA-1具有最低δ51V值,为−0.34‰±0.06‰,其余标准物质的δ51V值变化范围为−0.72‰~−0.81‰,均落在MORB范围内。本文对这些标准物质钒同位素组成的报道,丰富了钒同位素研究的标准物质数据库,有助于未来在更多领域开展钒同位素研究。

关键词：钒同位素火成岩 MC-ICP-MS 标准物质高精度分析方法

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Asymptotic stability of solutions to the nonisentropic hydrodynamic model for semiconductors

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Applied Mathematics(A Journal of Chinese Universities) 2008年第2期23卷 151-167页

作者： XU Jiang fang dao-yuan Dept. of Math. Nanjing Univ. of Aeronautics and Astronautics Nanjing 210016 China Dept. of Math. Zhejiang Univ. Hangzhou 310027 China

In this paper, the asymptotic stability of smooth solutions to the multidimensional nonisentropic hydrodynamic model for semiconductors is established, under the assumption that the initial data are a small perturbation of the stationary solutions for the thermal equilibrium state, whose proofs mainly depend on the basic energy methods.

关键词： asymptotic stability smooth solutions hydrodynamic model semiconductor.

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Global Existence of Small Solutions for Cubic Quasi-linear Klein-Gordon Systems in One Space Dimension

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Acta Mathematica Sinica,English Series 2006年第4期22卷 1085-1102页

作者： dao Yuan fang Ru Ying XUE Department of Mathematics Zhejiang University Hangzhou 310027 P. R. China

In this paper, we consider a system of two cubic quasi-linear Klein-Gordon equations with different masses for small, smooth, compactly supported Cauchy data in one space dimension. We show that such a system has global existence when the nonlinearities satisfy a convenient null condition. Our results extend the global existence proved by Sunagawa recently under the non-resonance assumption to that under the resonance assumption.

关键词： Cubic Quasi-linear Klein-Gordon systems One space dimension Global existence Asymptotic behavior

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Analytical solution for 1D consolidation of unsaturated soil with mixed boundary condition

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第1期14卷 61-70页

作者： Zhen-dong SHAN dao-sheng LING Hao-jiang ding MOE Key Lab of Soft Soils and Geo-environmental Engineering Zhejiang University Institute of Engineering Mechanics China Earthquake Administration

Based on consolidation equations proposed for unsaturated soil, an analytical solution for 1D consolidation of an unsaturated single-layer soil with nonhomogeneous mixed boundary condition is developed. The mixed boundary condition can be used for special applications, such as tests occur in laboratory. The analytical solution is obtained by assuming all material parameters remain constant during consolidation. In the derivation of the analytical solution, the nonhomogeneous boundary condition is first transformed into a homogeneous boundary condition. Then, the eigenfunction and eigenvalue are derived according to the consolidation equations and the new boundary condition. Finally, using the method of undetermined coefficients and the orthogonal relation of the eigenfunction, the analytical solution for the new boundary condition is obtained. The present method is applicable to various types of boundary conditions. Several numerical examples are provided to investigate the consolidation behavior of an unsaturated single-layer soil with mixed boundary condition.

关键词： Unsaturated soil Consolidation Mixed boundary condition Analytical solution

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