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Reference genes identified in the silkworm Bombyx moil during metamorphism based on oligonucleotide microarray and confirmed by qRT-PCR

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Insect Science 2008年第5期15卷 405-413页

作者： Gen-Hong Wang Qing-You Xia dao-jun cheng jun Duan Ping Zhao Jie Chen Li Zhu Institute of Agriculture and Life Sciences Chongqing University the Key Sericultural Laboratory of Agricultural Ministry School of Biotechnology Southwest University Chongqing China

Gene expression quantification at mRNA level is very important for postgenomic studies, as gene expression level is the reflection of the special biological function of the target gene. Methods used for gene expression quantification, such as microarray or quantitative real-time polymerase chain reaction （qRT-PCR）, require stable expressed reference genes. Thus, finding suitable control genes is essential for gene quantification. In this study, a genome-wide survey of reference genes during metamorphism was performed on silkworm Bombyx mori. Twelve genes were chosen as putative reference genes based on a whole genome oligonucleotide microarray normalized by external controls. Then, qRT- PCR was employed for further validation and selection of potential reference gene candidates. The results were analyzed, and stable genes were selected using geNorm 3.4 and NormFinder software. Finally, considering factors from every aspect, translation initiation factor 4A, translation initiation factor 3 subunit 4, and translation initiation factor 3 subunit 5 （represented by sw22934, sw14876, and sw13956） were selected as reliable internal controls across the examined developmental stages, while cytoplasmic actin （sw22671）, the commonly used reference gene in a previous study was shown to vary drastically throughout the examined developmental stages. For future research, we recommend the use of the geometric mean of those three stable reference genes as an accurate normalization factor for data normalization of different developmental stages during metamorphism.

关键词： Bombyx mori microarray nomalization factor reference gene qRT-PCR

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Genome-wide identification of target genes for transcription factor BR-C in the silkworm,Bombyx mori

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Insect Science 2021年第6期28卷 1530-1540页

作者： Meng-Pei Guo Wen-Liang Qian Xue-Chuan He Jian Peng Peng Wang Wei-Na Wang Qing-You Xia dao-jun cheng State Key Laboratory of Silkworm Genome Biology Biological Science Research CenterSouthwest UniversityChongqing400715China Chongqing Key Laboratory of Sericultural Science Southwest UniversityChongqing400715China

Transcription factor Broad Complex(BR-C)is an ecdysone primary response gene in insects and participates in the regulation of insect growth and *** this study,we performed a genome-wide identification of BR-C target genes in silkworm(Bombyx mori)using chromatin immunoprecipitation followed by high-throughput sequencing(ChIP-seq).As a result,a total of 1006 BR-C ChIP peaks were identified,and 15%of peaks were located in the promoter regions of 133 protein-coding *** annotation revealed that these ChIP peak-associated genes,as potential BR-C targets,were enriched in pathways related to biosynthetic process,metabolic process,and *** analysis and quantitative real-time polymerase chain reaction(PCR)examination revealed that developmental changes in expression patterns of a portion of potential BR-C targets,including HR96 and GC-otl,were similar to those of ***-PCR examination confirmed that BR-C could directly bind to the promoters of potential ***,dual luciferase assays demonstrated that HR96 promoter activity was significantly upregulated following BR-C overexpression,and this upregu-lation was abolished when the binding motif in the promoter was *** study will be helpful for deciphering the regulatory roles of BR-C during insect growth and development.

关键词： BR-C ChIP-seq direct binding silkworm target genes transcriptional regulation

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CRISPR-Cas13-mediated RNA editing in the silkworm Bombyx mori

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Zoological Research 2024年第6期45卷 1249-1260页

作者： Yao-Hao Tang Xing Zhang Zong-Cai Dai Hao Li Yan Yang Tu-Jing Zhao Dong-Qin Yuan Wen-Liang Qian dao-jun cheng Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqing 400715China State Key Laboratory of Resource Insects Southwest UniversityChongqing 400715China

The CRISPR-Cas13 system,an RNA-guided editing tool,has emerged as a highly efficient and stable RNA editing *** the CRISPR-Cas13 system has been developed in several insect species,its application in lepidopterans has not yet been *** the present study,we evaluated the RNA cleavage activity of the CRISPR-Cas13 system in the silkworm(Bombyx mori),a model lepidopteran insect,both ex vivo and in *** established two stable silkworm BmE cell lines expressing PspCas13b and CasRx,*** analysis demonstrated that both PspCas13b and CasRx effectively down-regulated the transcription of exogenouslyintroduced target and endogenous genes in these cell *** addition,we generated two transgenic silkworm strains,one expressing CasRx and the other expressing RNA-guided CRISPR RNA targeting Sex combs reduced(Scr).Further crossing experiments showed that CasRx induced a down-regulation of Scr transcription in silkworms,which impaired systemic growth of ***,this study demonstrated that the CRISPR-Cas13RNA editing system works efficiently in the silkworm,providing a potential alternative approach for RNA manipulation in lepidopteran insects.

关键词： Silkworm CRISPR PspCas13b CasRx RNA editing

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The silkworm homolog of Methoprene-tolerant （Met） gene reveals sequence conservation but function divergence

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Insect Science 2010年第4期17卷 313-324页

作者： Zhi-Qing Li dao-jun cheng Ling Wei Ping Zhao Xu Shu Lin Tang Zhong-Huai Xiang Qing-You Xia The Key Sericultural Laboratory of Agricultural Ministry Southwest University Chongqing School of Life Science Southwest University Chongqing The Institute of Agronomy and Life Sciences Chongqing University Chongqing China

Methoprene-tolerant （Met） gene has been found to be involved in juvenile hormone （JH） action in insects. Herein, we isolated a silkworm （Bombyx mori） homolog of Met gene from Drosophila melanogaster using bio-informatics analysis and rapid amplification of cDNA ends - polymerase chain reaction method, and defined it as BmMet. The full-length cDNA ofBmMet gene consists of 1 917 nucleotides and includes a 1 368 bp of open reading frame for a deduced protein of 455 amino acids. All deduced protein sequences from Met genes in B. mori and other surveyed insects contain four typical domains ofbHLH, PAS-A, PAS-B and PAC, highlighting a high sequence conservation of Met genes during insect evolution. Also, genomic structure and phylogenic analysis suggested that Met in both B. mori and Drosophila species may originate from an ancestor gene with gce, another member of bHLH-PAS family, via gene duplication. In addition, BmMet was detected in all surveyed tissues and throughout the whole life of silkworm at transcriptional levels. Furthermore, silkworm individuals with RNAi silencing of BmMet gene in the early stage of the fourth instar larvae could molt normally and pupate successfully. This result was different from the observation in T. castaneum but similar to that in D. melanogaster after Met knockdown, revealing that the action mode of Met in B. mori and D. melanogaster should be divergent with that in other insect species.

关键词： Bombyx mori expression pattern genomic structure Methoprene-tolerantgene RNAi

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Global expression profile of silkworm genes from larval to pupal stages： Toward a comprehensive understanding of sexual differences

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Insect Science 2011年第6期18卷 607-618页

作者： Min Zhao Xing-Fu Zha Jin Liu Wen-Ji Zhang Ning-Jia He dao-jun cheng Ya Dai Zhong-Huai Xiang Qing-You Xia Institute of Sericulture and Systems Biology College of Biotechnology Southwest University Chongqing Institute of Agronomy and Life Science Chongqing University Chongqing China Tobacco Chuanyu Industrial Corporation Chengdu China

Sexual dimorphism is a widespread phenomenon in many higher animals. The genes and gene networks that underlie sex differences are poorly understood. Using microarray data we analyzed sex-related differences in the global expression profiles of silkworm genes from larval to pupal stages. Sex-biased genes could be divided into three clusters. Cluster 1 contained 932 genes that showed a female-biased expression trend at first and a male-biased trend afterward. Cluster 2 included 283 male-biased genes. Cluster 3 was comprised of 497 female-biased genes that were expressed during the late pupal stage. Cluster 1 genes were found to be related closely to cuticle proteins, hormones, binding proteins, enzyme regulators, structural proteins, transcription regulators and so on. Several genes in clusters 2 and 3 were associated with spermatogenesis and oogenesis, respectively. The chromosomal distribution of sex-biased genes showed evidence of chromosomal enrichment. In particular a large number of the silkworms＇ male-biased genes are located on the Z chromosome. These results provide new insights into the molecular differences that dictate sexual dimorphism in the silkworm.

关键词： Bombyx mori microarray sex-biased gene sexual dimorphism

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Observation of topological charge transformations in acoustic vortex using passive periodic systems

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 140-147页

作者： Di-Chao Chen Xie Liu Changqing Xu Da-Jian Wu Ying cheng Xiao-jun Liu School of Physics and Technology Nanjing Normal University Key Laboratory of Modern Acoustics School of Physics Nanjing University

Vortex transmutation utilizes a periodic system, which not only enables the alteration of the topological charge(TC) of unstable input vortices but also facilitates their transformation into stable structures. This approach is crucial for investigating the characteristics of vortices in various wave systems. Here, we achieve acoustic vortex TC transmutation by employing a welldesigned dual-structured plate external system(DPES). The interactions between the acoustic vortex and the finite-order discrete rotational symmetry lead to the transformation of its topological charge when complete rotational symmetry is broken to Nthorder discrete symmetry. Our results experimentally demonstrate the acoustic vortex TC transmutation based on passive artificial structures, enabling numerous applications in particle manipulation and information multiplexing as well as advancing our understanding and control over free-space acoustic vortices.

关键词： acoustic vortex topological charge transformation artificial structure

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理论探索二维X_(2)UO_(4)(X=K,Rb,Cs)的力学特征、电子结构和声子输运特征

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原子与分子物理学报 2025年第2期42卷 143-150页

作者：程钧湖北医药学院研究生院十堰442000

本文用基于密度泛函理论的第一性原理计算方法,研究了三种建议的二维材料X_(2)UO_(4)(X=K,Rb,Cs)的稳定性、弹性、电子结构和声子振动特性.计算结果显示,三种材料同时具备较好的热力学、动力学和机械稳定性.电子结构表明,三种材料属于... 详细信息

本文用基于密度泛函理论的第一性原理计算方法,研究了三种建议的二维材料X_(2)UO_(4)(X=K,Rb,Cs)的稳定性、弹性、电子结构和声子振动特性.计算结果显示,三种材料同时具备较好的热力学、动力学和机械稳定性.电子结构表明,三种材料属于宽禁带间接半导体,其计算能隙分别是3.90、3.79和3.42 eV,并且在300 K时有着71.31-174.23 cm^(2)/Vs的电子迁移率.声子输运特性表明,三种材料的声子色散均没有虚频出现,声学模和低频光学模呈现强烈耦合,且有着低频率low-lying声子,这使得材料具备很强的声子非谐效应、低声子群速度和强声子散射率,从而表现出很低晶格热导率,在300 K温度下时仅为0.16-0.19 W/mK.这些特性表明,二维X_(2)UO_(4)(X=K,Rb,Cs)在纳米电子和热电器件领域具备应用潜力.

关键词：二维材料能带迁移率声子热导率

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From blocker to booster:Harnessing garnet surface chemistry for advanced solid-state electrolytes

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Nano Research 2025年第1期18卷 323-331页

作者： jun cheng Xuan Zhou Hongqiang Zhang Zhen Zeng Yuanyuan Li Yulong Zhu Yingsheng Liao Lijie Ci Deping Li State Key Laboratory of Precision Welding and Joining of Materials and Structures School of Materials Science and EngineeringHarbin Institute of Technology(Shenzhen)Shenzhen 518055China

Garnet electrolytes with high ionic conductivity and electrochemical stability are widely used as fillers to fabricate composite solid electrolytes(CPEs)within polymer ***,the performance of CPEs is significantly influenced by the surface characteristics of the garnet ***,the impact of garnet surface characteristics on CPEs was systematically investigated and a conversion from a typically unstable and Lewis basic surface to a more stable Lewis acidic surface was realized,which is shown to be more conductive to the improved performance of *** simultaneously removing the Li_(2)CO_(3)layer and applying a Li-Al-O coating,the influence of surface characteristics on CPEs was *** Lewis acid Li-Al-O surface coating not only promotes lithium salt dissociation,improving the ionic conductivity and ionic transfer number,but also prevents the reformation of the passive Lewis basic Li_(2)CO_(3)*** to garnet with a Lewis basic Li_(2)CO_(3)surface,the garnet modified with a Lewis acid Li-Al-O coating enhances CPEs,which exhibit an improved critical current density of 1.0 mA·cm^(-2)and highly stable lithium symmetric cell cycling for 400 h at 0.2 mA·cm^(-2).This research highlights the importance of surface chemistry in the design of high-performance solid-state batteries and presents a strategic modification approach for garnetbased CPEs.

关键词： garnet electrolyte composite solid electrolyte surface chemistry all-solid-state batteries

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Moment Redistribution Effect of the Continuous Glass Fiber Reinforced Polymer-Concrete Composite Slabs Based on Static Loading Experiment

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Structural Durability & Health Monitoring 2025年第1期19卷 105-123页

作者： Zhao-jun Zhang Wen-Wei Wang Jing-Shui Zhen Bo-cheng Li De-cheng Cai Yang-Yang Du Hui Huang Offshore Oil Engineering Co.Ltd. Tianjin300461China Department of Bridge Engineering School of TransportationSoutheast UniversityNanjing211189China

This study aimed to investigate the moment redistribution in continuous glass fiber reinforced polymer(GFRP)-concrete composite slabs caused by concrete cracking and steel bar yielding in the negative bending moment *** experimental bending moment redistribution test was conducted on continuous GFRP-concrete composite slabs,and a calculation method based on the conjugate beam method was *** composite slabs were formed by combining GFRP profiles with a concrete layer and supported on steel beams to create two-span continuous composite slab *** methods,epoxy resin bonding,and stud connection,were used to connect the composite slabs with the steel *** experimental findings showed that the specimen connected with epoxy resin exhibited two moments redistribution phenomena during the loading process:concrete cracking and steel bar yielding at the internal *** contrast,the composite slab connected with steel beams by studs exhibited only one-moment redistribution phenomenon throughout the loading *** the concrete at the internal support cracked,the bending moment decreased in the internal support section and increased in the midspan *** the steel bars yielded,the bending moment further decreased in the internal support section and increased in the mid-span *** GFRP profiles do not experience cracking,there was no significant decrease in the bending moment of the mid-span *** test specimens experienced compressive failure of concrete at the mid-span *** results showed good agreement between the calculated and experimental values of bending moments in the mid-span section and internal support *** proposed model can effectively predict the moment redistribution behavior of continuous GFRP-concrete composite slabs.

关键词： Moment redistribution GFRP-concrete composite slabs bending moment experimental study analysis model

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Recyclable High-performance Carbon Fiber Reinforced Epoxy Composites Based on Dithioacetal Covalent Adaptive Network

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Chinese Journal of Polymer Science 2024年第10期42卷 1514-1524,I0011页

作者： Gui-Lian Shi Ting-cheng Li dao-Hong Zhang jun-Heng Zhang Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education&Hubei Key Laboratory of Catalysis and Materials Science Hubei R&D Center of Hyperbranched Polymers Synthesis and ApplicationsSouth-Central Minzu UniversityWuhan430074China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan430200China Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials Hubei UniversityWuhan430062Chin

Recycling of carbon fiber reinforced composites is important for sustainable development and the circular *** the use of dynamic chemistry,developing high-strength recyclable CFRPs remains a major challenge due to the mutual exclusivity between the dynamic and mechanical properties of ***,we developed a high-strength recyclable epoxy resin(HREP)based on dynamic dithioacetal covalent adaptive network using diglycidyl ether bisphenol A(DGEBA),pentaerythritol tetra(3-mercapto-propionate)(PETMP),and vanillin epoxy resin(VEPR).At high temperatures,the exchange reaction of thermally activated dithioacetals accelerated the rearrangement of the network,giving it significant reprocessing ***,HREP exhibited excellent solvent resistance due to the increased cross-linking *** this high-strength recyclable epoxy resin as the matrix and carbon fiber modified with hyperbranched ionic liquids(HBP-AMIM+PF6-)as the reinforcing agent,high performance CFRPs were successfully *** tensile strength,interfacial shear strength(IFSS)and interlaminar shear strength(ILSS)of the optimized formulation(HREP20/CF-HBPPF6)were 1016.1,70.8 and 76.0 MPa,*** addition,the CFRPs demonstrated excellent solvent and acid/*** CFRPs could completely degrade within 24 h in DMSO at 140℃,and the recycled CF still maintained the same tensile strength and ILSS as the original after multiple degradation cycles.

关键词： Epoxy resin Hyperbranched ionic liquid Recycling Carbon fiber Composites

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