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Increasingγ-CD conversion rates by improving thermostability of Bacillus ***-44876γ-CGTase

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Food Bioscience 2023年第1期51卷 346-354页

作者： Xiaoxiao Li danni zheng Jing Wu zhengyu Jin Birte Svensson Yuxiang Bai State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxiJiangsu214122China Enzyme and Protein Chemistry Department of Biotechnology and BiomedicineTechnical University of DenmarkDK-2800 KgsLyngbyDenmark

Poor thermostability is a limiting factor for applications ofγ-CGTase that affects starch utilization and yield ofγ-*** thermostability of Bacillus ***-44876γ-CGTase(BFγ-CGTase)was improved by addition of Ca^(2+)and site-directed ***,10 mM Ca^(2+)increased the half-life(t1/2)at 55 and 60℃ from 3.0 to 0.3 h to 17.4 and 2.0 h,*** spectra indicated that Ca^(2+)stabilized the tertiary structure of the BFγ-CGTase and hence improved the *** to serine of a glycine residue in anα-helix related to thermostability also improved the stability especially at the higher ***,in 10 mM Ca^(2+)the G208S mutant further increased t1/2 to 20.0 h and 4.0 h at 55 and 60℃,*** G208S mutant in 10 mM Ca^(2+)producedγ-CD from tapioca starch with 40%increased yield of that from BFγ-*** work involving rational protein engineering provided a new tool for enzymaticγ-CD production.

关键词： Starch Protein engineering Three-dimensional structure

The Incidence of Moderate and Severe Ovarian Hyperstimulation Syndrome in Hospitalized Patients in China

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Health Data Science 2023年第1期3卷 26-30页

作者： danni zheng Ying Shi Yuanyuan Wang Rong Li Xiaoyu Long Jie Qiao Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China. National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China. Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China. Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology Beijing 100191China. Beijing Advanced Innovation Center for Genomics Beijing 100871China. Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China. China Standard Medical Information Research Center ShenzhenChina.

Background:Ovarian hyperstimulation syndrome(OHSS)occurs in women receiving fertility *** and severe OHSS cases are required to be admitted to hospital for *** incidence of moderate and severe OHSS and the characteristics of these cases are unknown in *** aimed to assess the incidence of moderate and severe OHSS in national databases from China between 2013 and ***:We extracted moderate and severe OHSS cases from the Hospital Quality Monitoring System,the nationwide inpatient data collection *** used ovum pick-up(OPUbaidu)cycle data from the annual report of China’s National Health Commission,developed on the basis of OPU data collected by National ART Management Information *** incidence of moderate and severe OHSS(women aged 20 to 50 years)and year-specific incidence by each calendar year in China were *** also investigated the age distribution in OHSS and OHSS with different ***:We extracted 18,022 eligible patients with moderate or severe OHSS and 1,581,703 OPU *** overall incidence of moderate and severe OHSS between 2013 and 2017 was 1.14%.The year-specific moderate and severe OHSS incidence was 1.1%in 2013,1.4%in 2014,1.4%in 2015,1.1%in 2016,0.9%in 2017,*** aged 26 to 30 years accounted for 48.4%of OHSS cases,followed by women aged 31 to 35 years(30%)and 20 to 25 years(14.2%).The age distribution pattern was consistent across OHSS with different ***:This study reported the incidence of moderate and severe OHSS in China using nationwide data for the first *** findings support that women aged under 35 years receiving assisted reproductive technology need more attention than other age groups in terms of OHSS risk control.

关键词： stimulation admitted Ovarian

The emerging concept of travel therapy in health science:Will it be applied to tourists visiting sub-frigid climate zones?

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Frigid Zone Medicine 2022年第4期2卷 200-203页

作者： Jun Wen danni zheng Fangli Hu School of Business and Law Edith Cowan UniversityPerth 6027Australia Department of Tourism Fudan UniversityShanghai 200433China Centre for Precision Health Edith Cowan University Perth 6027Australia

Have you ever thought that taking a tourism-related trip could contribute to your well-being or even improve your health status?Have you considered that tourism research could play a role in health science by showcasing new ways of thinking?Tourism scholars have recently contemplated tourism's place in public/global health[1]as a means of enriching the well-being of people living with conditions such as dementia.A seminal study using an interdisciplinary approach[2]integrated expertise in tourism,psychology,marketing,and medical science and proposed tourism as a dementia intervention based on positive psychology.

关键词： travel tourism visiting

Current status of intravenous tissue plasminogen activator dosage for acute ischaemic stroke:an updated systematic review

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Stroke & Vascular Neurology 2018年第1期3卷 28-33页

作者： Xia Wang Shoujiang You Shoichiro Sato Jie Yang Cheryl Carcel danni zheng Sohei Yoshimura Craig S Anderson Else Charlotte Sandset Thompson Robinson John Chalmers Vijay K Sharma The George Institute for Global Health NewtownNew South WalesAustralia Sydney Medical School The University of SydneySydneyNew South WalesAustralia Department of Neurology The Second Affiliated Hospital of Soochow UniversitySuzhouChina Department of Cerebrovascular Medicine National Cerebral and Cardiovascular CenterOsakaJapan Department of Neurology The First Affiliated Hospital of Chengdu Medical CollegeChengduChina Royal Prince Alfred Hospital CamperdownNew South WalesAustralia The George Institute China Peking University Health Science CenterBeijingChina Department of Neurology Oslo University HospitalOsloNorway Department of Cardiovascular Sciences and NIHR Biomedical Research Unit for Cardiovascular Diseases University of LeicesterLeicesterUK Yong Loo Lin School of Medicine National University of SingaporeSingapore Division of Neurology National University HospitalSingapore

The optimal dose of recombinant tissue plasminogen activator(rtPA)for acute ischaemic stroke(AIS)remains controversial,especially in Asian *** aimed to update the evidence regarding the use of low-dose versus standard-dose *** performed a systematic literature search across MEDLINE,Embase,Cochrane Central Register of Controlled Trials(CENTRAL),PsycINFO and Cumulative Index to Nursing and Allied Health Literature(CINAHL)from inception to 22 August 2016 to identify all related *** outcomes were death or disability(defined by modified Rankin Scale 2-6),death,and symptomatic intracerebral haemorrhage(sICH).Where possible,data were pooled for meta-analysis with ORs and corresponding 95% CIs by means of random-effects or fixed-effects *** included 26 observational studies and 1 randomised controlled trial with a total of 23210 *** doses of rtPA were used for thrombolysis of AIS in ***-analysis shows that low-dose rtPA was not associated with increased risk of death or disability(OR 1.13,95% CI 0.95 to 1.33),or death(OR 0.86,95% CI 0.74 to 1.01),or decreased risk of sICH(OR 1.06,95% CI 0.65 to 1.72).The results remained consistent when sensitivity analyses were performed including only low-dose and standard-dose rtPA or only Asian *** review shows small difference between the outcomes or the risk profile in the studies using low-dose and/or standard-dose rtPA for ***-dose rtPA was not associated with lower risk of death or disability,death alone,or sICH.

关键词： dosage doses acute

从社会融合到社会融入:西方社群档案的建设之路

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档案与建设 2018年第7期1卷 26-30页

作者：郑丹妮上海大学图书情报档案系上海200444

论文运用文献分析法,认为社会融合是一个过程和目标导向的复合概念,可以划分为基于文化适应的社会融合和基于结构融合的社会融入两个阶段。而从社会融合向社会融入发展的过程正是西方社群档案的建设之路。在这一过程中,档案工作者的职... 详细信息

论文运用文献分析法,认为社会融合是一个过程和目标导向的复合概念,可以划分为基于文化适应的社会融合和基于结构融合的社会融入两个阶段。而从社会融合向社会融入发展的过程正是西方社群档案的建设之路。在这一过程中,档案工作者的职能有可能发生扩张,档案工作者可以成为社群档案教育家和政策分析师。

关键词：社群档案社会融合社会融入文化认同国家认同

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伦理道德发展影响会计信息质量的行为学研究探讨

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经济评论 2016年第2期198卷 151-160页

作者：刘颖斐郑丹妮武汉大学经济与管理学院

高质量的会计信息是资本市场稳定发展的基石。现有研究从诸多角度讨论了影响会计信息质量的因素,但仍缺乏对会计从业人员本身行为模式的研究,特别是伦理道德发展水平对其行为及会计信息质量影响方面的研究,这一点在我国的学术研究中表... 详细信息

高质量的会计信息是资本市场稳定发展的基石。现有研究从诸多角度讨论了影响会计信息质量的因素,但仍缺乏对会计从业人员本身行为模式的研究,特别是伦理道德发展水平对其行为及会计信息质量影响方面的研究,这一点在我国的学术研究中表现得尤为突出。本文认为,在原则导向的会计准则模式下,会计从业人员的职业道德水平高低将会影响其做出的会计判断,进而决定其所提供的会计信息质量。文章通过剖析国外相关研究,在对行为人借助伦理敏感度识别伦理困境、做出伦理判断、并在伦理动机的促使下最终形成伦理品质的过程进行较全面介绍的基础上,指出为了提高中国资本市场上的会计信息质量,更好地规范会计信息提供者的行为,有必要对我国制度背景下会计行为人的道德发展水平进行更为深入的研究。

关键词：会计信息质量伦理判断道德发展伦理困境

牙源性钙化囊肿与牙源性钙化上皮瘤的三维影像特点

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北京大学学报（医学版） 2024年第1期56卷 131-137页

作者：凌晓彤屈留洋郑丹妮杨静闫雪冰柳登高高岩北京大学口腔医学院·口腔医院影像科国家口腔医学中心国家口腔疾病临床医学研究中心口腔生物材料和数字诊疗装备国家工程研究中心口腔数字医学北京市重点实验室国家卫生健康委员会口腔医学计算机应用工程技术研究中心国家药品监督管理局口腔材料重点实验室北京100081 北京大学口腔医学院·口腔医院第一门诊部特诊科国家口腔医学中心国家口腔疾病临床医学研究中心口腔生物材料和数字诊疗装备国家工程研究中心口腔数字医学北京市重点实验室国家卫生健康委员会口腔医学计算机应用工程技术研究中心国家药品监督管理局口腔材料重点实验室北京100081 北京大学口腔医学院·口腔医院口腔病理科国家口腔医学中心国家口腔疾病临床医学研究中心口腔生物材料和数字诊疗装备国家工程研究中心口腔数字医学北京市重点实验室国家卫生健康委员会口腔医学计算机应用工程技术研究中心国家药品监督管理局口腔材料重点实验室北京100081

目的:利用螺旋CT和锥束计算机体层摄影术(cone-beam computed tomography,CBCT)比较分析牙源性钙化囊肿(calcifying odontogenic cyst,COC)与牙源性钙化上皮瘤(calcifying epithelial odontogenic tumor,CEOT)的三维影像学特征。方法:回顾性收集北京大学口腔医院经病理证实的19例COC和16例CEOT的螺旋CT或CBCT影像资料,结合患者的临床与病理表现分析其影像学特征,包括病变位置、大小、膨隆程度、内部结构及牙齿受累情况等,并对病变内钙化特点进行分类。结果:19例COC患者中,男性12例,女性7例,平均年龄27岁,89.5%(17/19)的病变位于前牙区和前磨牙区,100.0%存在颌骨膨隆,78.9%出现骨皮质不连续。16例CEOT患者中,男性3例,女性13例,平均年龄36岁,81.3%(13/16)的病变位于前磨牙区和磨牙区,56.3%存在颌骨膨隆,93.8%出现骨皮质不连续。根据病变内钙化物的分布特点分为无钙化型(未见钙化影像)、边缘型(钙化影位于病变边缘,且仅在一侧散在分布)、弥散型(多发钙化影广泛分布于病变范围内)、团块型(存在直径大于5 mm的钙化团块)及冠周型(钙化影像聚集于阻生牙周围)。73.7%的COC病变区存在钙化,包括边缘型9例、弥散型3例及团块型2例;42.8%的CEOT病变区存在钙化,包括弥散型2例及冠周型5例。另外,6例COC病变中存在牙瘤样影像,9例无钙化的CEOT中8例为朗格汉斯(Langerhans)型,病变较小(近远中径平均为17.8 mm),不含阻生牙,且无牙根吸收。结论:COC好发于颌骨前部,膨隆明显,而CEOT好发于颌骨后部,多存在骨皮质不连续。两者的钙化特点差异较大,COC病变区钙化影像发生率高,多沿病变边缘散在分布,位于病变一侧,远离阻生牙,部分病变与牙瘤共同发生;CEOT逾半数无钙化且病变较小,其余病变中钙化物影像多围绕在阻生牙周围。

关键词：牙源性钙化囊肿牙源性钙化上皮瘤三维成像锥束计算机体层摄影术

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γ-CGTase酶学性质及产物特异性影响因素

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食品与发酵工业 2020年第5期46卷 38-45页

作者：郑丹妮柏玉香纪杭燕李晓晓王禹蔣彤金征宇江南大学食品学院江苏无锡214122 食品科学与技术国家重点实验室(江南大学) 江苏无锡214122

将γ-环糊精葡萄糖基转移酶(γ-CGTase)的特有区域作为判断依据,在美国国家生物信息中心(National Center for Biotechnology Information,NCBI)数据库中,通过序列比对及生物信息学分析,筛选出一种新型基因,并将其克隆表达得到一种酶。... 详细信息

将γ-环糊精葡萄糖基转移酶(γ-CGTase)的特有区域作为判断依据,在美国国家生物信息中心(National Center for Biotechnology Information,NCBI)数据库中,通过序列比对及生物信息学分析,筛选出一种新型基因,并将其克隆表达得到一种酶。利用高效液相色谱-质谱联用进行产物鉴定,表明该酶为γ-CGTase;酶分子质量大约为80 kDa,最适温度50℃,最适pH 10.0;无α-环糊精生成;甲醇、乙醇、环己烷、十二醇激活γ-CGTase活力,异丙醇和正丁醇抑制γ-CGTase活力;反应时间、淀粉质量浓度及乙醇体积分数均会影响γ-CGTase的产物特异性。HPLC结果显示,在10%的乙醇最终体积分数条件下,γ-CGTase催化产物中γ-环糊精的比例由40.11%增加至78.20%,专一性提高了94.96%。该研究提供了一种新型的γ-CGTase,为提高γ-CGTase产物特异性提供研究基础。

关键词： γ-环糊精葡萄糖基转移酶酶学性质乙醇产物特异性

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Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala:association with enhanced cued fear expression

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Neural Regeneration Research 2025年第11期20卷 3259-3272页

作者： Huidong Li Faqin Li Zhaoyi Chen Erwen Wu Xiaoxi Dai danni Li Haojie An Shiyi Zeng Chunyan Wang Li Yang Cheng Long School of Life Sciences South China Normal UniversityGuangzhouGuangdong ProvinceChina School of Life Sciences Guangzhou UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research Panyu Central HospitalGuangzhouGuangdong ProvinceChina

Neuronal activity,synaptic transmission,and molecular changes in the basolateral amygdala play critical roles in fear ***(CYLD)is a deubiquitinase that negatively regulates the nuclear factor kappa-B *** is well studied in non-neuronal cells,yet underinvestigated in the brain,where it is highly *** studies have shown involvement of CYLD in the remodeling of glutamatergic synapses,neuroinflammation,fear memory,and anxiety-and autism-like ***,the precise role of CYLD in glutamatergic neurons is largely ***,we first proposed involvement of CYLD in cued fear *** next constructed transgenic model mice with specific deletion of Cyld from glutamatergic *** results show that glutamatergic CYLD deficiency exaggerated the expression of cued fear in only male ***,loss of CYLD in glutamatergic neurons resulted in enhanced neuronal activation,impaired excitatory synaptic transmission,and altered levels of glutamate receptors accompanied by over-activation of microglia in the basolateral amygdala of male ***,our study suggests a critical role of glutamatergic CYLD in maintaining normal neuronal,synaptic,and microglial *** may contribute,at least in part,to cued fear expression.

关键词： basolateral amygdala cued fear expression cylindromatosis deubiquitinase glutamate receptor 1 glutamatergic neuron microglial activation N-methyl-D-aspartate receptor 1 neuronal activation synaptic transmission

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