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Effect of Optical Brightening Agent(OBA)on Bleaching Process on Paper of Eucalyptus Kraft Pulp

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Natural Resources 2013年第5期4卷 378-382页

作者： Bianca Moreira Barbosa Fernando Gomes Jorge Colodette danila Carvalho Mauro Manfredi Department of Chemistry Federal University of VicosaVicosaBrazil Department of Forestry Engineering Federal University of VicosaVicosaBrazil

In integrated factories of pulp and paper, the bleaching control is made by kappa number and/or brightness. However, the product paper is evaluated and valued for its whiteness. The level of final brightness of pulp certainly affects the demand for optical brighteners in paper manufacturing, nevertheless, there is not a direct correlation between consumption of optical brightener and final brightness, being the method of bleaching pulp, especially from the final stage of bleaching, also important in relation to this demand. Therefore, this study aimed to evaluate the potential of using the parameter whiteness as a tool to control bleaching pulp, instead of the traditional brightness and evaluate the impacts of different methods of bleaching and the pulp final brightness level in action of optical brighteners, to improve their performance. It was observed that the whiteness and brightness of the pulp are highly correlated and that the control of the bleaching plant can be done by any one of these two parameters. It was also concluded that, considering current prices of bleaching reagents and of optical brighteners, it is better to bleach the pulp up to lower brightness values (e.g. 88% ISO) and use larger amounts of optical brighteners to brighten the mass until the whiteness of 130 CIE. This conclusion is valid for whiteness measured before the size press. Further studies are needed to validate this conclusion considering the finished product.

关键词： Eucalyptus Bleaching Kraft Pulping Optical Brightening Agent(OBA)

Effect of Mineral Fertilization and Irrigation on Sunflower Yields

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American Journal of Plant Sciences 2015年第7期6卷 870-879页

作者： Lucia Helena Garófalo Chaves danila Lima Araujo Hugo Orlando Carvallo Guerra Walter Esfrain Pereira Federal University of Campina Grande Avenue Aprigio Veloso Campina Grande Brazil Federal University of Paraiba Campus II Areia Brazil

Among the cultures used for the production of biofuels, the sunflower is one of the most important. Although some information exists, the water and nutritional needs of sunflower in the north east of Brazil are not well known. To fill knowledge gaps, an experiment was carried out to evaluate the effect of nitrogen (N), phosphorus (P), potassium (K) fertilization and available soil water (ASW) on sunflower yields. The sunflower cultivar Embrapa 122-V2000 was subjected to 44 treatments on a completely randomized design generated by the Baconian Matrix with four rates of N (0, 60, 80 and 100 kg·ha-1), four rates of P2O5 (0, 80, 100 and 120 kg·ha-1), four rates of K2O (0, 80, 100 and 120 kg·ha-1), and four available soil water (ASW) levels (55%, 70%, 85% and 100%) replicated three times. Urea was used as a source of N, triple super phosphate as P and potassium chloride as K. In all the experimental units was applied 2 kg·B·ha-1 as boric acid. The components of production evaluated were dry matter of the head, total number of achenes, total achenes’ weight and 1000 achenes’ weight. The results of this research showed that nitrogen had a significant effect on the dry matter of the head, total number of achenes and total achenes’ weight. Phosphorus affected all production components and potassium affected the total number and the weight of achenes. With the exception of the 1000 achenes’ weight, all the production components of the sunflower increased with the increased ASW level influenced significantly at 0.01 level of probably the total number of achenes. The highest rates of N, P and K (100, 120 and 120 kg·ha-1, respectively) and 100% of available soil water produced the highest production.

关键词： Water Fertilizers Yields Oil Plants Seeds

Targeting hormone-resistant breast cancer cells with docetaxel:a look inside the resistance

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Cancer Drug Resistance 2023年第1期6卷 103-115页

作者： Alexander M.Scherbakov Anna A.Basharina danila V.Sorokin Ekaterina I.Mikhaevich Iman E.Mizaeva Alexandra L.Mikhaylova Tatiana A.Bogush Mikhail A.Krasil’nikov Department of Experimental Tumor Biology Blokhin N.N.National Medical Research Center of OncologyMoscow 115522Russian Federation Group of Molecular Tumor Markers Blokhin N.N.National Medical Research Center of OncologyMoscow 115522Russian Federation

Aim:The study aims to analyze the effect of long-term incubation of ERα-positive MCF7 breast cancer cells with 4-hydroxytamoxifen(HT)on their sensitivity to tubulin polymerization inhibitor ***:The analysis of cell viability was performed by the MTT *** expression of signaling proteins was analyzed by immunoblotting and flow ***αactivity was evaluated by gene reporter *** establish hormone-resistant subline MCF7,breast cancer cells were treated with 4-hydroxytamoxifen for 12 ***:The developed MCF7/HT subline has lost sensitivity to 4-hydroxytamoxifen,and the resistance index was *** Akt activity(2.2-fold)and decreased ERαexpression(1.5-fold)were revealed in MCF7/HT *** activity of the estrogen receptorαwas reduced(1.5-fold)in MCF7/*** of class Ⅲβ-tubulin expression(TUBB3),a marker associated with metastasis,revealed the following trends:higher expression of TUBB3 was detected in triple-negative breast cancer MDA-MB-231 cells compared to hormone-responsive MCF7 cells(P<0.05).The lowest expression of TUBB3 was found in hormone-resistant MCF7/HT cells(MCF7/HT

关键词： Cancer docetaxel 4-hydroxytamoxifen classⅢβ-tubulin resistance breast cancer estrogen receptors alpha

3D imaging lipidometry in single cell by in-flow holographic tomography

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Opto-Electronic Advances 2023年第1期6卷 10-25页

作者： Daniele Pirone Daniele Sirico Lisa Miccio Vittorio Bianco Martina Mugnano danila del Giudice Gianandrea Pasquinelli Sabrina Valente Silvia Lemma Luisa Iommarini Ivana Kurelac Pasquale Memmolo Pietro Ferraro CNR-ISASI Institute of Applied Sciences and Intelligent Systems“E.Caianiello”Via Campi Flegrei 34PozzuoliNapoli 80078Italy Department of Electrical Engineering and Information Technologies University of Naples“FedericoⅡ”via Claudio 21Napoli 80125Italy Department of Chemical Materials and Production Engineering of the University of Naples FedericoⅡPiazzale Tecchio 80Napoli 80125Italy Department of Mathematics and Physics University of Campania“Luigi Vanvitelli”Caserta 81100Italy Biotechnology and Methods in Laboratory Medicine Department of ExperimentalDiagnostic and Specialty Medicine(DIMES)University of BolognaBologna 40126Italy Subcellular Nephro-Vascular Diagnostic Program Pathology Unit S.Orsola IRCCSUniversity of BolognaBologna 40126Italy Unit of Medical Genetics Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Study and Research Center on Gynecological Neoplasias Department of Medical and Surgical Sciences(DIMEC)University of BolognaVia Massarenti 9Bologna 40138Italy Center for Applied Biomedical Research(CRBA) University of BolognaBologna 40138Italy Department of Pharmacy and Biotechnology(FABIT) University of BolognaBologna 40126Italy

The most recent discoveries in the biochemical field are highlighting the increasingly important role of lipid droplets(LDs)in several regulatory mechanisms in living *** are dynamic organelles and therefore their complete characterization in terms of number,size,spatial positioning and relative distribution in the cell volume can shed light on the roles played by *** now,fluorescence microscopy and transmission electron microscopy are assessed as the gold standard methods for identifying LDs due to their high sensitivity and ***,such methods generally only provide 2D assays and partial ***,both can be destructive and with low productivity,thus limiting analysis of large cell numbers in a *** we demonstrate for the first time the capability of 3D visualization and the full LD characterization in high-throughput with a tomographic phase-contrast flow-cytometer,by using ovarian cancer cells and monocyte cell lines as models.A strategy for retrieving significant parameters on spatial correlations and LD 3D positioning inside each cell volume is *** information gathered by this new method could allow more in depth understanding and lead to new discoveries on how LDs are correlated to cellular functions.

关键词： lipid droplets label-free phase-contrast imaging in-flow tomography 3D imaging

Comparative evaluation of clinical effectiveness of different regimens in patients with concomitant cardiac pathology

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哈尔滨医科大学学报 2018年第1期52卷 95-97页

作者： Julia V.Luneva Sergey V.Povetkin HE Jian-gang danila I.Kolomiets YAO Lan Department of Clinical Pharmacology Kursk State Medical University Department of International Office Harbin Medical University Student of Medical Faculty Kursk State Medical University

Objective To compare the clinical effectiveness of different scheme of pharmacotherapy in treatment of combined cardiac pathology. Methods The was completed comparative analysis of different regimens using various ACE inhibitors(perindopril and fosinopril) in patients with combined cardiovascular *** The results of treatment showed improvement in hemodynamic and clinical parameters. Conclusion The results of the study point to the pronounced clinical efficacy of combined therapy in both groups of patients with concomitant cardiac pathology.

关键词：不同方案心脏病理疗效评价

Hybrid Method for the Formation of Biocomposites on the Surface of Stainless Steel Implants

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Engineering（科研） 2012年第10期4卷 613-618页

作者： Sergei I. Tverdokhlebov Viktor P. Ignatov Igor B. Stepanov Denis O. Sivin danila G. Petlin Tomsk Polytechnic University Tomsk Russia

This study reports a hybrid method which allows the formation of biocomposites on stainless steel implants. The main idea of the method is to create multilayer coatings consisting of titanium primer layer and a microarc calcium-phosphate coating. The titanium layer is deposited from plasma of continuous vacuum-arc discharge, and calcium-phosphate coating is formed by the microarc oxidation technique. The purpose of the hybrid method is to combine the properties of good strength stainless steel with high bioactivity of calcium-phosphate coating. This paper describes the chemical composition, morphology characteristics, adhesion and the ability of the formed biocomposites to stimulate the processes of osteoinduction. It is expedient to use such biocomposites for implants which carry heavy loads and are intended for long-term use, e.g. total knee endoprosthesis.

关键词： Biocomposite Stainless Steel Titanium Vacuum-Arc Deposition of Coatings Short-Pulse High-Frequency Plasma-Immersion Ion Implantation Microarc Oxidation Implant

Grafting vigour is associated with DNA de-methylation in eggplant

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Horticulture Research 2021年第1期8卷 3311-3320页

作者： Elisa Cerruti Carmina Gisbert Hajk-Georg Drost danila Valentino Ezio Portis Lorenzo Barchi Jaime Prohens Sergio Lanteri Cinzia Comino Marco Catoni Department of Agricultural Forest and Food SciencesPlant Genetics and BreedingUniversity of TorinoGrugliascoItaly The Sainsbury Laboratory University of CambridgeCambridgeUK Institute for Conservation&Improvement of Valencian Agrodiversity(COMAV) Universitat Politècnica de ValènciaValenciaSpain Computational Biology Group Department of Molecular BiologyMax Planck Institute for Developmental BiologyTübingenGermany School of Biosciences University of BirminghamBirminghamUnited Kingdom Institute for Sustainable Plant Protection National Research Council of ItalyTorinoItaly

In horticulture,grafting is a popular technique used to combine positive traits from two different *** is achieved by joining the plant top part(scion)onto a rootstock which contains the stem and *** can provide resistance to stress and increase plant production,but despite their wide use,the biological mechanisms driving rootstock-induced alterations of the scion phenotype remain largely *** that epigenetics plays a relevant role during distance signalling in plants,we studied the genome-wide DNA methylation changes induced in eggplant(Solanum melongena)scion using two interspecific rootstocks to increase *** found that vigour was associated with a change in scion gene expression and a genome-wide hypomethylation in the CHH ***,this hypomethylation correlated with the downregulation of younger and potentially more active long terminal repeat retrotransposable elements(LTR-TEs),suggesting that graft-induced epigenetic modifications are associated with both physiological and molecular phenotypes in grafted *** results indicate that the enhanced vigour induced by heterografting in eggplant is associated with epigenetic modifications,as also observed in some heterotic hybrids.

关键词： younger driving grafted

Modification in self-rated health in patients discharged by a geriatric rehabilitation ward

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Health 2013年第6期5卷 94-98页

作者： Eleonora Marelli Giuseppe Procino Maria Cottino Giorgio Previderè Silvio Giorgi Davide Dell’Acqua Santina Bruno Marco Cairati danila Ferrari Rosella Capuano Mauro Colombo Geriatric Institute “Camillo Golgi” Milan Italy Geriatric Institute “Camillo Golgi” Milan Italy

Self-rated health is a valuable outcome in geriatric rehabilitation besides objective results. The present work aims at measuring and analyzing overall health as it is perceived at admission into and at discharge from a geriatric rehabilitation ward. Overall health was self-appraised through a visual-analogue scale (VAS), spanning from 0 (worst) to 10 (best). We studied 1997 patients (70% females), aged 79 (standard deviation, s.d. 8.7) years;most were frail, either functionally, clinically and cognitively. 80% of patients were discharged to home after a length of stay lasting 47.5 (s.d. 22.7) days. At admission, 3/5 patients appraised favorably their overall health (VAS ≥ 6/10): at discharge, the proportion rose to 3/4, with a mean (s.d.) gain = 2 (2) points. The improvement in self-perceived health score positively correlates with the grade expressing clients’ overall satisfaction for the stay (p 0.001), and with discharge versus admission differences in: Barthel Index (BI) total score (p < 0.001), Tinetti total score, Mini Mental State Examination (MMSE), Geriatric Depression Scale (5?items GDS), pain (VAS 0 to 10). A Linear regression model predicting the changes in self-perceived health included changes in BI, MMSE, GDS, pain, dropping Tinetti test. Changes in self-rated health were positively correlated to functional gain adjusted for pre-morbid level, and to relative functional gain. By analysis of variance, health self-appraisal changed more favorably in patients discharged to home than for other social outcomes (all p < 0.001). As expected, trends in self-perceived health parallel improvements in objective functional gauges and subjective indicators.

关键词： Cognition Functional Gains Geriatric Rehabilitation Mood Pain Self-Rated Health

Surface Characterization of Titanium Implants Treated in Hydrofluoric Acid

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Journal of Biomaterials and Nanobiotechnology 2012年第1期3卷 87-91页

作者： danila M. Korotin Stefan Bartkowski Ernst Z. Kurmaev Manfred Meumann Eugeniya B. Yakushina Ruslan Z. Valiev Seif O. Cholakh Faculty of Physics University of Osnabrueck Osnabrueck Germany Institute of Metal Physics Russian Academy of Sciences-Ural Division Yekaterinburg Russia Institute of Physics of Advanced Materials Ufa State Aviation Technical University Ufa Russia Ural Federal University Yekaterinburg Russia

The results of XPS measurements of commercially pure titanium cp-Ti) before and after chemical treatment are presented. We have measured XPS spectra of core levels (Ti 2p, O 1s, C 1s, F 1s) and valence bands of coarse-grained cp-Ti before and after standard acid treatment accepted in dentistry (in 1% HF and 40% HF for 1 min). It is found, that acid treatment of cp-Ti reduces the content of hydrocarbons increasing the surface energy and bio-compatibility of Ti-implants. On the other hand, it is fixed that oxygen concentration on the surface of the acid treated cp-Ti is much higher than for the untreated sample, because the acid treatment removes the contaminated surface layers, increases their reactivity, provides a better passivation and formation of thick protecting TiO2 layer.

关键词： Commercially Pure Titanium Surface Composition Hydrofluoric Acid Surface Characterization Biocompatibility