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Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer

Cancer Communications 2024年第1期44卷 101-126页

作者： Monika Raab Izabela Kostova Samuel Peña-Llopis daniela Fietz Monika Kressin Seyed Mohsen Aberoumandi Evelyn Ullrich Sven Becker Mourad Sanhaji Klaus Strebhardt Department of Gynecology Medical SchoolGoethe-UniversityFrankfurt am MainGermany Translational Genomics in Solid Tumors West German Cancer CenterUniversity HospitalEssenGermany German Cancer Consortium(DKTK) EssenGermany German Cancer Research Center(DKFZ) HeidelbergGermany Histology and Embryology Institute for Veterinary AnatomyGiessenGermany Franfurt Cancer Institute(FCI) Goethe UniversityFrankfurt am MainGermany German Cancer Consortium(DKTK) Partner site Frankfurt/Mainza partnership between DKFZ and University Hospital FrankfurtFrankfurt am MainGermany Experimental Immunology Department for Children and Adolescents MedicineUniversity Hospital FrankfurtGoethe UniversityFrankfurt am MainGermany

Background:The cellular tumor protein p53(TP53)is a tumor suppressor gene that is frequently mutated in human *** various cancer types,the very aggressive high-grade serous ovarian carcinoma(HGSOC)exhibits the high-est prevalence of TP53 mutations,present in>96%of *** intensive efforts to reactivate p53,no clinical drug has been approved to rescue p53 *** this study,our primary objective was to administer in vitro-transcribed(IVT)wild-type(WT)p53-mRNA to HGSOC cell lines,primary cells,and ortho-topic mouse models,with the aim of exploring its impact on inhibiting tumor growth and dissemination,both in vitro and in ***:To restore the activity of p53,WT p53 was exogenously expressed in HGSOC cell lines using a mammalian vector ***,IVT WT p53 mRNA was delivered into different HGSOC model systems(primary cells and patient-derived organoids)using liposomes and studied for proliferation,cell cycle progression,apoptosis,colony formation,and chromosomal *** alterations induced by p53 mRNA were analyzed using RNA sequencing in OVCAR-8 and primary HGSOC cells,followed by ingenuity path-way *** vivo effects on tumor growth and metastasis were studied using orthotopic xenografts and metastatic intraperitoneal mouse ***:Reactivation of the TP53 tumor suppressor gene was explored in differ-ent HGSOC model systems using newly designed IVT mRNA-based *** introduction of WT p53 mRNA triggered dose-dependent apoptosis,cell cycle arrest,and potent long-lasting inhibition of HGSOC cell *** analysis of OVCAR-8 cells upon mRNA-based p53 reactivation revealed significant alterations in gene expression related to p53 signaling,such as apoptosis,cell cycle regulation,and DNA *** p53 function concurrently reduces chromosomal instability within the HGSOC cells,under-scoring its crucial contribution in safeguarding genomic integrity by moderating the baselin

关键词： apoptosis cell cycle chromosomal instability HGSOC metastatic intraperitoneal mouse model HGSOC orthotopic Xenograft model high-grade serous ovarian cancer in vitro-transcribed p53-mRNA liposomal IVT mRNA delivery system patient-derived organoid patient-derived primary cancer cell

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意大利语版护理管理者胜任力测评工具的信效度检验

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International Journal of Nursing Sciences 2024年第3期11卷 338-348页

作者： Dhurata Ivziku Lucia Filomeno daniela Forte Rosario Caruso Gianluca Conte Arianna Magon Raffaella Gualandi Marco Di Muzio daniela Tartaglini Department of Health Professions Fondazione Policlinico Universitario Campus Bio-MedicoRomeItaly Department of Biomedicine and Prevention University of Rome Tor VergataRomeItaly Health Professions Research and Development Unit IRCCS San Donato HospitalMilanItaly Department of Biomedical Sciences for Health University of MilanMilanItaly Department of Clinical and Molecular Medicine Sapienza UniversityRomeItaly Vice President Italian Society for the Direction and Management in Nursing(SIDMI) RomeItaly

目的旨在翻译Chase护理管理者胜任力测评工具(the Chase Nurse Manager Competency Instrument,CNMCI)为意大利语,并对其进行信度和效度检验。方法对英文版CNMCI进行翻译,并通过横断面研究验证其信效度。采用预翻译(建立等效)、初始翻... 详细信息

目的旨在翻译Chase护理管理者胜任力测评工具(the Chase Nurse Manager Competency Instrument,CNMCI)为意大利语,并对其进行信度和效度检验。方法对英文版CNMCI进行翻译,并通过横断面研究验证其信效度。采用预翻译(建立等效)、初始翻译、预测试、审查和管理5个步骤的问卷翻译方法,将英文版工具翻译为意大利语版。于2022年8月到2023年6月,选取意大利3个地区31所医疗机构349名护理管理者进行调查。采用内容效度、结构效度评估工具的效度;采用Cronbach'sα系数、重测信度评估工具的信度。结果意大利语版CNMCI,包括“知识理解”和“实施和/或使用能力”两个分量表,每个分量表包括53个条目。工具的条目内容效度指数(I-CVI)为0.83~1.00,两个分量表的内容效度指数(S-CVI)均为0.97。经验证性因子分析显示该工具各项拟合指数表现良好。工具中“知识理解”分量表的Cronbach'sα系数为0.978,“实施和/或使用能力”分量表的Cronbach'sα系数为0.976。各个维度之间的相关系数为0.494~0.908重测信度为0.820,表明工具的一致性较好。结论意大利语版CNMCI在测量护理管理者胜任力方面显示出良好的信度和效度,并支持该工具的多维度结构。此外,研究结果支持了这些因素的层次性质,进一步支持了该工具的有效性。

关键词：胜任力护理管理者信度调查及问卷效度

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The effect of Laves phases and nano-precipitates on the electrochemical corrosion resistance of Mg-Al-Ca alloys under alkaline conditions

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Journal of Magnesium and Alloys 2024年第6期12卷 2447-2461页

作者： Markus Felten Veronika Chaineux Siyuan Zhang Ali Tehranchi Tilmann Hickel Christina Scheu Joshua Spille Marta Lipińska-Chwałek Joachim Mayer Benjamin Berkels Marcus Hans Imke Greving Silja Flenner Sandra Sefa daniela Zander Chair of Corrosion and Corrosion Protection RWTH Aachen UniversityAachen 52072 Germany Max-Planck-Institut für Eisenforschung GmbH Düsseldorf 40237 Germany Central Facility for Electron Microscopy RWTH Aachen UniversityAachen 52074 Germany Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons Forschungszentrum Jülich GmbHJülich 52428Germany Mathematical Image and Signal Processing Aachen Institute for Advanced Study in Computational Engineering ScienceRWTH Aachen UniversityAachen 52062 Germany Materials Chemistry RWTH Aachen UniversityAachen 52074 Germany Institute of Materials Physics Helmholtz-Zentrum HereonGeesthacht 21502 Germany

The electrochemical corrosion mechanisms of Mg alloys were extensively studied in previous investigations of different chemical com-positions,modified surface states and various electrolyte ***,recent research focused on the active state of Mg dissolution,leading to unresolved effects of secondary phases adjacent to a stableα-solid solution passive *** present study investigates the fundamental electrochemical corrosion mechanisms of three different Laves phases with varying phase morphologies and phase fractions in the passive state of Mg-Al-Ca *** microstructure was characterized by(transmission-)electron microscopy and synchrotron-based transmission X-ray *** electrochemical corrosion resistance was determined with a standard three-electrode setup and advanced in-situ flow cell measurements.A new electrochemical activity sequence(C15>C36>α-Mg>C14)was obtained,as a result of a stable passive layer formation on theα-solid ***,nm-scale Mg-rich precipitates were identified within the Laves phases,which tend to inhibit the corrosion kinetics.

关键词： Laves phase STEM Magnesium Corrosion Passive layer

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Dielectric spectroscopy characterization of Naþ ion-conducting polymer nanocomposite system PEO–PVP–NaIO_(4)–TiO_(2)

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Journal of Advanced Dielectrics 2024年第1期14卷 59-73页

作者： Georgi B.Hadjichristov daniela G.Kovacheva Yordan G.Marinov daniela B.Karashanova Todor E.Vlakhov Nicola Scaramuzza Georgi Nadjakov Institute of Solid State Physics Bulgarian Academy of Sciences 72 Tzarigradsko Chaussee Blvd.BG-1784 SofiaBulgaria Institute of General and Inorganic Chemistry Bulgarian Academy of Sciences Acad.Georgi Bonchev Str. 11 bd.BG-1113 SofiaBulgaria Institute of Optical Materials and Technologies“Acad.Jordan Malinowski” Bulgarian Academy of Sciences Acad.Georgi Bonchev Str.bl.109BG-1113 SofiaBulgaria Dipartimento di Fisica Universita degli Studi della Calabria Via P.BucciCubo 33BRende(CS)IT-87036Italy

We studied the effect of titanium dioxide(TiO_(2))nanoparticles(NPs)on dielectric behavior of Naþion-conducting salt-complexed polymer nanocomposite system formed from a binary polymer blend of poly(ethylene oxide)(PEO)and polyvinyl pyrrolidone(PVP),with the addition of both sodium metaperiodate(NaIO_(4))at concentration 10 wt.%and TiO_(2) NPs of size10 nm,at concentrations 1,2,3,4 and 5 wt.%.Free standing nanocomposite PEO/PVP/NaIO_(4)/TiO_(2) films(150m)were characterized at room-temperature by analyzing their complex electrical impedance and dielectric spectra in the range 1 Hz–1 *** the concentration of 3 wt.%of TiO_(2) NPs,both ion conductivity and dielectric permittivity of the PEO/PVP/NaIO_(4)/TiO_(2) ion-conducting dielectrics reach an enhancement by more than one order of magnitude as compared to nanoadditive-free case.

关键词： Dielectric properties sodium-ion-polymer electrolyte systems titanium dioxide(TiO_(2))nanoparticles nanocomposites KWW model

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Two-dimensional atomically thin Pt layers on MXenes: The role of electronic effects during catalytic dehydrogenation of ethane and propane

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Nano Research 2024年第3期17卷 1251-1258页

作者： Zhe Li Tobias K.Misicko Fan Yang Xiaopeng Liu Zhenwei Wu Xiaoyang Gao Tao Ma Jeffrey T.Miller daniela S.Mainardi Collin D.Wick Zhenhua Zeng Yang Xiao Yue Wu Department of Chemical and Biological Engineering Iowa State University618 Bissell RoadAmesIA 50011USA Institute for Micromanufacturing Louisiana Tech University505 Tech DriveRustonLA 71272USA Davidson School of Chemical Engineering Purdue University480 Stadium Mall DriveWest LafayetteIN 47907USA Michigan Center for Materials Characterization University of Michigan2800 Plymouth RdAnn Arbor MI 48109USA College of Engineering and Science Louisiana Tech UniversityRustonLA 71272USA

Atomically thin Pt nanolayers were synthesized on the surface of Mo2TiC2 MXenes and used for the catalytic dehydrogenation of ethane and propane into ethylene and propylene,two important chemicals for the petrochemical *** compared with Pt nanoparticles,the atomically thin Pt nanolayer catalyst showed superior coke-resistance(no deactivation for 24 h),high activity(turnover frequencies(TOFs)of 0.4–1.2 s^(-1)),and selectivity(>95%)toward ethylene and *** unique Pt nanolayer has a similar geometric surface to Pt nanoparticles,enabling the investigations of the electronic effect on the catalytic performance,where the geometric effect is *** is found that the electronic effect plays a critical role in dehydrogenative product selectivity and catalyst *** metal–support interaction is found dependent on the substrate and metal components,providing wide opportunities to explore high-performance MXene-supported metallic catalysts.

关键词： MXene catalysis alkane dehydrogenation nanolayers

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Determination of Metal Content in Soil and Wheat Plant by Inductively Coupled Plasma Mass Spectrometry

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Journal of Agricultural Chemistry and Environment 2024年第3期13卷 300-311页

作者： Iubu (Badea) Anişoara Ion V. Popescu Claudia Stihi Ioana daniela Dulama Doctoral School of Physics University of Bucharest Magurele Romania Faculty of Science and Arts Valahia University of Targoviste Targoviste Romania Academy of Romanian Scientists Bucharest Romania Institute of Multidisciplinary Research for Science and Technology Valahia University of Targoviste Targoviste Romania

This study aims to investigate the level of soil pollution and the grade of accumulation of metals and heavy metals by wheat plants from the soil in different parts of the crop: root, stem, leaf, spike and grain. Sampling campaigns took place in February, April and July when wheat plants were at different growth stages. A number of eight soil samples and eight wheat plant samples were collected. The sampled wheat plant was taken at the same time and from the same place as the soil. Concentrations of Al (aluminium), Cr (chromium), Mn (manganese), Fe (iron), Ni (nickel), Co (cobalt), Cu (copper), Zn (zinc), Sr (strontium), Cd (cadmium) and Pb (lead) were determined by inductively coupled plasma mass spectrometry. Bioconcentration and translocation factors were calculated for the samples analysed.

关键词： Soil Wheat Plant Heavy Metals Translocation Factor

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Age and petrogenesis of the newly discovered Early Permian granite in the Kumtor gold field， Kyrgyz Tien-Shan

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Journal of Earth Science 2024年

作者： Kyiazbek Asilbekov Rustam Orozbaev Etienne Skrzypek Christoph Hauzenberger Elena Ivleva daniela Gallhofer Jian-Feng Gao Nikolay Pak Anatoliy Shevkunov Anatoliy Bashkirov Aizat Zhaanbaeva Institute of Geology， National Academy of Sciences of the Kyrgyz Republic Institute of Earth Sciences， University of Graz State Key Laboratory of Ore Deposit Geochemistry， Institute of Geochemistry， Chinese Academy of Sciences Kumtor Gold Company CJSC

Permian intrusions are widespread in the Middle and Southern Tien Shan， with fewer occurrences in the Northern Tien-Shan. Notably， many of these intrusions are spatially associated with a variety of ore deposits， indicating a significant link between magmatic activity and mineralization processes in these areas. We studied granite samples recently recovered from drilling in the Kumtor gold field to evaluate their potential relationships with gold mineralization. We report major and trace element geochemistry， zircon U-Pb age and Hf isotope data for this so-called Kumtor granite. The Kumtor granite is metaluminous to peraluminous and belongs to the high-K and calc-alkaline series with I-type geochemical characteristics. The relatively high K2O and Na2O concentrations and low high field strength elements （HFSE） and heavy rare earth elements （HREE）， the presence of biotite within these I-type granites， together with their low zircon saturation temperatures （731–779 °C）， suggest that they were likely derived from a hydrous source formed by dehydration melting of mica-bearing， medium- to high-K metabasaltic rocks. Our zircon U-Pb dating results indicate that the Kumtor granite intruded at 293 ± 1.7 Ma， which is consistent with the age range of other Middle Tien-Shan granitoids. The zircon Hf isotopic composition is εHf（t）= –7.56 to –5.05， indicating an ancient （1.39 to 1.52 Ga） crustal origin. Petrographical， geochemical and geochronological data indicate that the Kumtor granite is similar to leuco-granites of the Terekty complex. Our results indicate that the Kumtor granite was emplaced in the early Permian in a post-collision setting and may have temporal and genetic relationships with gold mineralization.

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ICF框架下运动疗法对慢性非特异性颈痛效果的Meta分析

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中国康复理论与实践 2024年第7期30卷 778-788页

作者：温艳飞杨露班玥 Ykabaru daniela BERBESI NORIEGA 张郝琪王丽刘华首都体育学院北京市100191

目的基于《国际功能、残疾和健康分类》(ICF)框架,系统评价运动疗法对慢性非特异性颈痛(CNSNP)患者身体结构、功能、活动和参与等方面的干预效果。方法构建PICO架构,系统检索CBM、万方、维普、中国知网、Cochrane Library、Embase、Pu... 详细信息

目的基于《国际功能、残疾和健康分类》(ICF)框架,系统评价运动疗法对慢性非特异性颈痛(CNSNP)患者身体结构、功能、活动和参与等方面的干预效果。方法构建PICO架构,系统检索CBM、万方、维普、中国知网、Cochrane Library、Embase、PubMed和Web of Science等中、英文数据库,纳入有关运动疗法干预CNSNP患者的随机对照试验,时间范围为建库至2024年3月。采用Cochrane偏倚风险评估工具和物理治疗证据数据库(PEDro)量表对纳入文献进行质量评价,采用GRADE对结局指标进行证据质量评价,采用RevMan 5.3进行数据合并和分析,采用Stata 18.0进行偏倚风险评估。结果最终纳入11篇文献,共668例患者。PEDro量表评分5~8分。运动疗法类型包括肌力训练、稳定性训练、本体感觉训练、瑜伽和普拉提等;对照组包括空白对照、物理因子治疗和健康教育。运动疗法可显著增加患者颅椎角(SMD=0.84, 95%CI 0.42~1.26, P <0.001),降低疼痛视觉模拟量表评分(SMD=-2.05,95%CI-2.58~-1.52, P <0.001),提高压力疼痛阈值(MD=112.27, 95%CI 75.03~149.50, P <0.001),提高颈椎前屈(SMD=1.24, 95%CI 0.34~2.15, P=0.007)、侧屈(SMD=1.52, 95%CI 0.40~2.65, P=0.008)关节活动度,提高颈深屈肌耐力(SMD=1.02, 95%CI 0.10~1.94, P=0.03),改善颈椎关节位置觉(SMD=-1.00,95%CI-1.47~-0.53, P <0.001),但对于改善后屈(SMD=0.85, 95%CI-1.04~2.75, P=0.38)、旋转(SMD=1.65, 95%CI-0.35~3.65, P=0.11)关节活动度的效果不显著;运动疗法还能降低患者颈椎功能障碍指数评分(MD=-11.88, 95%CI-16.09~-7.68, P <0.001),对健康调查简表评分效果不显著(MD=19.04, 95%CI-3.00~41.08, P=0.09)。结论运动疗法可以改善CNSNP患者的异常姿势、疼痛、前屈和侧屈运动、颈屈肌耐力和关节位置觉,改善功能障碍,但对提高颈部后屈和旋转运动,改善生活质量方面仍需进一步的研究。

关键词：慢性非特异性颈痛运动国际功能、残疾和健康分类 Meta分析

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星巴克人手不足导致顾客不满

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商业周刊（中文版） 2024年第10期 12-14页

作者： daniela Sirtori 孟洁冰(译) 不详

星巴克的员工表示,人手不足导致顾客等待时间升、订单未完成和销量下降。星巴克的咖啡师说,他们正面临一个令人担忧的问题:饮品订单接而至,但制作饮品的人却越来越少。据员工和经理透露,在一些门店,一年前还有五个人制作冰玛奇朵和双份... 详细信息

星巴克的员工表示,人手不足导致顾客等待时间升、订单未完成和销量下降。星巴克的咖啡师说,他们正面临一个令人担忧的问题:饮品订单接而至,但制作饮品的人却越来越少。据员工和经理透露,在一些门店,一年前还有五个人制作冰玛奇朵和双份浓缩咖啡,而现在只剩下三四个人,这些知情人因未获授权公开发言而要求不具名。

关键词：星巴克顾客等待时间门店顾客不满不具名知情人饮品订单

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Laparoscopic central pancreatectomy with gastro-pancreatic anastomosis for symptomatic serous cystadenoma:A case report and literature review

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Laparoscopic, Endoscopic and Robotic Surgery 2024年第2期7卷 87-91页

作者： Giuseppe Frazzetta Antonino Picciurro Angela Maffongelli Irene Vitale Francesco Vitale daniela Scimeca Michele Amata Anna Calì Ambra Bonaccorso Barbara Scrivo Vincenzo Di Martino Elisabetta Conte Filippo Mocciaro Roberto Di Mitri Pierenrico Marchesa Oncologic Surgery Unit ARNAS Civico HospitalPalermo 90100Italy Gastroenterology Unit ARNAS Civico HospitalPalermo 90100Italy

Surgery for lesions of the proximal part of the pancreatic body or neck can be challenging,and when enucleation is not possible,central pancreatectomy is an *** central pancreatic resection is rarely described worldwide;it is considered a difficult procedure mainly because of the risk of double pancreatic fistula developing at two sites of ***,it seems to be an excellent alternative to distal pancreatectomy or pancreaticoduodenectomy,with the advantages of preserving functioning parenchyma and reducing endocrine and exocrine ***,patients with pancreatic lesions requiring central resection are often managed with the open approach in many hospitals due to the complexity of total laparoscopic central pancreatectomy,which requires advanced laparoscopic skills,expertise and ***,we report a case of a 29-year-old female who underwent total laparoscopic central pancreatic resection with gastro-pancreatic anastomosis for symptomatic serous *** discuss the details of case management and review the relevant literature.

关键词： Laparoscopic Pancreatic surgery Central pancreatectomy Cystadenoma

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