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Variations in porcine colostrum oligosaccharide composition between breeds and in association with sow maternal performance

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Journal of Animal Science and Biotechnology 2020年第3期11卷 830-840页

作者： Paolo Trevisi Diana Luise Savanna Won Jaime Salcedo Micol Bertocchi daniela barile Paolo Bosi Department of Agricultural and Food Sciences University of BolognaViale G.Fanin 4640127 BolognaItaly Department of Food Science and Technology University of California-DavisOne Shields AvenueDavisCA 95616USA

Background:Oligosaccharides(OS)are indigestible carbohydrates naturally found in *** composition of porcine colostrum OS may influence the growth and the health of the neonate and consuming optimal concentrations of OS may reduce piglet susceptibility to *** this manner,targeted supplementation of animal feed with OS is being explored as a health management tool in the livestock *** variation in OS composition between different breeds of pig and its association with the litter performance is currently *** aim of this study was to characterize the colostrum OS composition from sows of different breed and parity and correlate this data with sow maternal ***:Eighty-three colostrum samples from parities 1 to 8 were gathered from 3 different breeds of sow:44 Large White sows,27 Landrace sows and 12 Duroc *** were taken between the birth of the first and the last piglet from sows that were not pharmacologically induced to *** were purified from the samples and analysed by MALDI-ToF mass spectrometry(21 OS compositions detected).The farrowing season and the maternal data were recorded for each sow,including the number of live piglets and the litter body weight at birth,at day(d)3 and at ***:Five OS compositions,including isomers of the bifidogenic Sialyllactose,Lacto-N-Tetraose and Lacto-NHexaose series,were detected in all the *** other OS were identified in at least 50%of samples,and their abundances were affected by breed(P<0.05;6 of 12),marginally affected by season(P<0.10;3 of 12)and never by parity *** abundances of each OS component were standardized by Z-score scaling(μ=0 and SD=1),transformed by principal component analysis,and four similarity clusters were *** membership was associated with litter weight gain within 3 days(P=0.063)and at weaning(P<0.05),but not with piglet mortality within 3 ***:OS composition of colostrum may partially explain the vari

关键词： MALDI-ToF analysis Milk Nutrient composition Pig breeds Piglet survival

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Rapid Quantification of Functional Carbohydrates in Food Products

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Food and Nutrition Sciences 2014年第1期5卷 71-78页

作者： Annabelle Le Parc Hyeyoung Lee Kevin Chen daniela barile Department of Food Science and Technology University of California Davis USA Department of Food Science and Technology University of California Davis USA Department of Food Science and Technology University of California Davis USA Foods for Health Institute University of Cali- fornia Davis USA.

Current research on milk bioactive components, including complex oligosaccharides, stimulated development of novel milk-based ingredients;this in turn sparked the development of methods that are simultaneously simple and sensitive. Oligosaccharides and glycoproteins present interesting health benefits, including antibacterial and antiviral effects, stimulation of the immune system, and participation in the establishment of a balanced gut microbiome in infants. This work describes the application of a simple and rapid method—Total Carbohydrate Assay—to the determination of functional carbohydrate content in various dairy-based functional products. The miniaturization and optimization of the carbohydrate quantification on microplates afforded good repeatability and sensitivity. The optimized method consumed only minimal amounts of reagents and samples, and carbohydrates were detected in the range from 0 - 20 μg. This assay was successfully applied to determine the content of complicated oligosaccharide mixtures and N-glycans in dairy-derived products. Several complementary analytical techniques were applied to confirm the results. This method is faster and far less expensive than mass spectrometry and it gives a general picture of complex carbohydrate concentrations for instances in which detailed data are not required as needed for research in discrete differences among various biological samples. The ability to quantify glycans in novel food products will provide a unique understanding of the potential of these novel ingredients for use by the food industry.

关键词： Carbohydrates Milk Colorimetric Method Microplate Mass Spectrometry

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早产母乳中内源性肽释放量高于其在足月生产母乳中的含量

早产母乳中内源性肽释放量高于其在足月生产母乳中的含量

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作者： David C Dallas Christina J Smink Randall C Robinson Tian Tian Andres Guerrero Evan A Parker Jennifer T Smilowitz Kasper A Hettinga Mark A Underwood Carlito B Lebrilla J Bruce German daniela barile 徐美虹 Department of Food Science and Technology University of CaliforniaDavisDavisCA Foods for Health Institute University of CaliforniaDavisDavisCA Dairy Science and Technology Group Wageningen University and Research Centre Department of Chemistry University of CaliforniaDavisDavisCA

当母亲自己的乳汁不适于婴儿喂养时,捐赠人乳(DHM)是早产儿营养的首选来源。但是DHM通常属于晚期哺乳期乳汁,对于早产儿可能不能提供充足的营养,因此了解足月儿和早产儿不同哺乳期母乳中的成分差异是十分重要的。鉴于肽是一种母乳的替...

当母亲自己的乳汁不适于婴儿喂养时,捐赠人乳(DHM)是早产儿营养的首选来源。但是DHM通常属于晚期哺乳期乳汁,对于早产儿可能不能提供充足的营养,因此了解足月儿和早产儿不同哺乳期母乳中的成分差异是十分重要的。鉴于肽是一种母乳的替代成分,已知足月儿母乳中的总乳蛋白浓度明显高于早产儿,并且随着哺乳期延长而逐渐降低,因此该研究推测在足月儿和早产儿的整个哺乳期,母乳中内生肽的组成、肽的功能和对这些肽负有责任的酶是有差异的。该研究从荷兰人乳库选取22份样本,对其中的总蛋白、肽组成、肽功能和酶进行了分析。分析选取的样本包括了早产儿

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