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Detection of Gluten in Dishes Offered as Gluten-Free in a Gastronomic Area Located in San José, Costa Rica

Natural Science 2022年第12期14卷 524-531页

作者： Vanessa Bagnarello-Madrigal daniela rodríguez-chaves Mayra Villegas-Barakat Andrés Chacón-Robles Moisés Coto-Hernández Escuela de Fisioterapia Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Escuela de Nutrición Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Facultad de Microbiología Universidad de Ciencias Médicas (UCIMED) San José Costa Rica Unidad del Aseguramiento de la Calidad Universidad de Ciencias Médicas (UCIMED) San José Costa Rica

Celiac disease, gluten sensitivity and gluten intolerance are health conditions that require consumers to avoid their gluten intake. In Costa Rica, Law 8975 regulates the amount of gluten permitted in prepackaged products labeled as “gluten-free”, but the parameters to be followed are not mandated for food service operations. This study investigated whether restaurants with gluten-free (GF) options in a gastronomic area of the city of San José, Costa Rica, achieved the requirements of the legislation that the dishes offered should contain less than 20 ppm gluten. Using data collected from five restaurants offering GF dishes, two provided dishes with quantities of gluten greater than 20 ppm (restaurant A, four samples and restaurant B, three samples);particularly dishes from the category “meat with sauce”. Although those dishes are naturally gluten-free, when they are handled in areas of shared production, they are exposed to the risk of cross contact, furthermore the adding of misscellaneous such as: marinades, sauces and condiments, which could contain traces of gluten or hidden gluten, a risk associated with the use of ingredients without certification GF (supplier’s practices and label declaration). Therefore improvements in food service procedures should be enforced. The findings of the present study emphasize the need to include restaurant foods in the relevant legislation to ensure that the gluten-intolerant sector of the population remains safe when eating out.

关键词： Celiac Disease Gluten-Free (GF) Gliadin Shared Production Cross Contact

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Antimalarial Activity of an Alkaloid Mixture Isolated from the Tender Leaves of the Nectandra membranacea （Laureaceae）

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Journal of Life Sciences 2018年第3期12卷 134-140页

作者： Vanessa Bagnarello-Madrigal daniela rodríguez-chaves Javier Alpizar-Cordero Alonso Calvo-Vargas Maribel Cordero-Villalobos Misael Chinchilla-Carmona Idalia Valerio-Campos Ronald Sánchez -Porras Departamento de Química Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Departamento de Investigación Básica Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Sección de Biología Sede Occidente Universidad de Costa Rica San José 10108 Costa Rica

Despite the breadth of knowledge about malaria, its transmission, preventive measures and available treatments and its difficulty in controlling the vector and the rapid spread of resistance to current drugs, there is a stimulus to develop new research looking for active molecules. The phytochemical studies of the active extract confirmed the presence of alkaloids, flavonoids, coumarins, tannins, reducing compounds, anthocyanins, terpenes, triterpenes and steroids. In the present work, it was possible to isolate a mixture of isoquinolinic alkaloids from the tender leaves of Nectandra membranacea （Laureaceae） with antimalarial activity. The mixture composed of （-） - gliazovine and （＋） - aplogliazovine showed an IC50 value of 32.18 μg/mL against the P. berghei NK65 strain. It is important to continue with these studies and their chemical components isolated from N. membranacea that could be used as an alternative the treatment of malaria.

关键词： Malaria Nectandra membranacea alkaloids phytochemical screening Laureaceae.

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Detection of Gluten in Dishes Offered as Gluten-Free in a Gastronomic Area Located in San José, Costa Rica

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Natural Science（自然科学期刊） 2022年第12期

作者： Vanessa Bagnarello-Madrigal daniela rodríguez-chaves Mayra Villegas-Barakat AndrésChacón-Robles Moisés Coto-Hernández

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Socio-spatial organization reveals paternity and low kinship in the Honduran white bat(Ectophylla alba)in Costa Rica

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Integrative Zoology 2021年第5期16卷 646-658页

作者： Edgar G.GUTIÉRREZ Isabela VIVAS-TORO daniela CARMONA-RUÍZ David VILLALOBOS-chaves Bernal rodrÍguez-HERRERA Melina Del REAL-MONROY Gloria LEÓN-AVILA Jorge ORTEGA Laboratorio de Bioconservación y Manejo Posgrado en Ciencias QuimicobiológicasDepartamento de ZoologíaEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Department of Biology University of WashingtonSeattleWashingtonUSA Escuela de Biología Universidad de Costa RicaSan JoséCosta Rica Laboratorio de Genómica Evolutiva Doctorado en Ciencias BásicasUniversidad Autónoma de ZacatecasZacatecasZac.México Laboratorio de Genética Posgrado en Ciencias QuimicobiológicasDepartamento de ZoologíaEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico

Ectophylla alba is a tent-making bat that roosts in mixed-sex clusters comprising adults and *** goal was to determine the genetic identity of individuals belonging to different roosting *** tested the hypothesis of kin selection as a major force structuring group *** used 9 microsatellites designed for *** to determine the genetic identity and probability of parentage of *** analyzed parentage and kinship using the software ML-Relate,GenAIEx,and *** obtained relationship probabilities(0.5)revealed a clear maternal relationship between female adults and offspring with allele compatibility,and at least 5 relationships between male adults and *** found a low degree of relatedness within roosting *** roosting groups at different sites,the mean probability of a half-sibling relationship ranged from 0.214 to 0.244 and,for full-sibling relationship,from 0.383 to ***,adult individuals were poorly related within clusters,and kinship as an evolutionary force could not explain group membership.

关键词： Ectophylla alba group composition kinship microsatellites relatedness

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Integration of an Objective Approach Focused on the Consumer to Evaluate the Quality of Processed Brussels Sprouts

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Food and Nutrition Sciences 2011年第9期2卷 1011-1017页

作者： daniela Flavia Olivera Sonia Zulma Viña Ricardo Mario Ferreyra Alicia Mugridge Rodolfo Horacio Mascheroni Alicia Raquel chaves CIDCA(Centro de Investigación y Desarrollo en Criotecnología de Alimentos) CONICET La PlataUniversidad Nacional de La Plata(UNLP)La PlataArgentina MODIAL(Unidad de Investigación y Desarrollo en Modelado y Diseño en Ingeniería de Alimentos) Facultad de IngenieríaUniversidad Nacional de La Plata(UNLP)La PlataArgentina

Few research works focus on integration of the objective analysis of frozen vegetable quality and the preference of consumers for these products. The aim of the present work was to investigate the consumption patterns of Brussels sprouts, the degree of importance that consumers give to main attributes, and the constructions they make about product quality. For this aim, a series of surveys (snowball sampling method) were performed with consumers from four cities in Argentina. Also, the effect of different industrial operations (two blanching procedures, freezing and cooking) on texture, color, total chlorophyll content and ascorbic acid content of processed sprouts, was determined with respect to the fresh product. A function Z (overall quality) was defined to integrate the information obtained by both approaches. Taking into account the relative importance assigned by surveyed consumers to appearance, texture and nutrient content, the quality of final product obtained from different processing methods was equivalent. Most consumers, 96% of total, preferred the fresh product, which suggests that prejudging by consumers influences attempts at objective estimation of quality.

关键词： Frozen Vegetables Brassica Oleracea L.Gemmifera Quality Attributes Quality Perception

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