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美国医学会眼科杂志（中文版） 2006年第4期18卷 248-249页

作者： Burkhard Dasch Andrea Fuhs Thomas Behrens Astrid Meister Juergen wellmann Manfred Fobker daniel Pauleikhoff Hans-werner Heine 刘亮（译）

目的：评价纤维蛋白原的血浆浓度和高灵敏度c反应蛋白与年龄相关性黄斑病变的关联。方法：从Muenster衰老和视网膜研究的基线检查中．选取一组人群共1060个人．年龄59到82岁。其中，873人（82％）有双眼眼底照、纤维蛋白原和cRP的完整... 详细信息

目的：评价纤维蛋白原的血浆浓度和高灵敏度c反应蛋白与年龄相关性黄斑病变的关联。方法：从Muenster衰老和视网膜研究的基线检查中．选取一组人群共1060个人．年龄59到82岁。其中，873人（82％）有双眼眼底照、纤维蛋白原和cRP的完整数据。潜在的疑似者也包括在横断面分析中。主要结果通过多元线性回归分析测量纤维蛋白原、cRP和年龄相关性黄斑病变的相关性。

关键词：年龄相关性黄斑病变视网膜免疫标志物横断面调查多元线性回归分析纤维蛋白原 c反应蛋白

Superior conjugative Plasmids Delivered by Bacteria to Diverse Fungi

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BioDesign Research 2022年第1期2022卷 151-165页

作者： Ryan R.cochrane Arina Shrestha Mariana M.Severo de Almeida Michelle Agyare-Tabbi Stephanie L.Brumwell Samir Hamadache Jordyn S.Meaney daniel P.Nucifora Henry Heng Say Jehoshua Sharma Maximillian P.M.Soltysiak cheryl Tong Katherine Van Belois Emma J.L.walker Marc-AndréLachance Gregory B.Gloor David R.Edgell Rebecca S.Shapiro Bogumil J.Karas Department of Biochemistry Schulich School of Medicine and DentistryThe University of Western OntarioLondonONCanada N6A 5C1 Department of Molecular and cellular Biology University of GuelphGuelphONCanada N1G 2W1 Department of Biology The University of Western OntarioLondonOntarioCanada N6A 5B7

Fungi are nature’s recyclers,allowing for ecological nutrient cycling and,in turn,the continuation of life on *** fungi inhabit the human microbiome where they can provide health benefits,while others are opportunistic pathogens that can cause ***,members of the fungal kingdom,have been domesticated by humans for the production of beer,bread,and,recently,medicine and ***,the great untapped potential exists within the diverse fungal ***,many yeasts are intractable,preventing their use in biotechnology or in the development of novel treatments for pathogenic ***,as a first step for the domestication of new fungi,an efficient DNA delivery method needs to be ***,we report the creation of superior conjugative plasmids and demonstrate their transfer via conjugation from bacteria to 7 diverse yeast species including the emerging pathogen candida *** create our superior plasmids,derivatives of the 57 kb conjugative plasmid pTA-Mob 2.0 were built using designed gene deletions and insertions,as well as some unintentional ***,a cluster mutation in the promoter of the conjugative gene traJ had the most significant effect on improving conjugation to *** addition,we created Golden Gate assembly-compatible plasmid derivatives that allow for the generation of custom plasmids to enable the rapid insertion of designer genetic ***,we demonstrated that designer conjugative plasmids harboring engineered restriction endonucleases can be used as a novel antifungal agent,with important applications for the development of next-generation antifungal therapeutics.

关键词： insertion continuation harbor

Discovery of Potent, Selective and Reversible caspase-3 Inhibitors

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合成化学 2004年第Z1期12卷 13-13页

作者： André Giroux John colucci christopher I. Bayly daniel J. Mckay Sophie Roy Steve Xanthoudakis John Vaillancourt Dita M. Rasper John Tam Paul Tawa Donald w. Nicholson Robert J. Zamboni Department of Medicinal chemistry Department of Biochemistry and Molecular Biology Merck Frosst Centre for Therapeutic Research Merck Frosst Canada & Co. P.O. Box 1005 Pointe-Claire-DorvalQuebec Canada H9R 4P8

Recent studies towards understanding the molecular mechanisms of apoptosis have revealed the importance of a group of cysteinyl aspartate specific proteases, the caspases, in the programmed cell death process (Hengartner, M.O. Nature 2000, 407, 770). caspase-3, in particular,has been characterized as the dominant effector caspase involved in the proteolytic cleavage of a variety of protein substrates including cytoskeletal proteins, kinases and DNA repair enzymes during apoptosis (Nicholson, D. w. cell Death Differ. 1999, 6, 1028). The development of potent and selective caspase-3 inhibitors has thus emerged as an attractive therapeutic target. In the presentation,the identification of a series of potent, selective and reversible non-peptidyl caspase-3 inhibitors containing a pyrazinone core (1) will be presented. SAR optimization at R1, R2, R3 and R4 led to the discovery of inhibitors such as 2 with excellent in vitro activities (Ic50 against rh-caspase-3: 5 nM; Ic50 against camptothecin induced apoptotic cell death in NT2 cells: 20 nM). compounds such as 2 also displayed excellent in vivo activities in a number of animal models of acute injuries (see: Methot, N. et al, J. Exp. Med. 2004, 119, 199; Toulmond, S. et al, British J. Pharm. 2004, 141,689; holtzman,D.M. et al, JBc, 2002, 277, 30128), and selected examples will be discussed during the presentation.

关键词： programmed cell death cytoskeletal proteins molecular mechanisms identification of animal models DNA repair in vitro

解糖微小寄生菌的菌株间差异对其寄生行为的影响

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解糖微小寄生菌的菌株间差异对其寄生行为的影响

第十六次全国牙体牙髓病学学术大会

作者：聂杰 daniel R·Utter 李永亮王晓燕何雪松 Jeffrey McLean Batbileg Bor 北京大学口腔医学院牙体牙髓科 Department of Organismic and Evolutionary Biology Harvard University Department of Periodontics University of Washington

目的:解糖微小寄生菌（Saccharibacteria）专性寄生于宿主细菌表面,形成独特的"细菌-细菌"寄生关系,并可能在牙周炎等口腔疾病中发挥重要作用。作为近年才得以分离培养的新微生物,较少的可培养菌株数量限制了对其寄生行为的深入理解。...

目的:解糖微小寄生菌（Saccharibacteria）专性寄生于宿主细菌表面,形成独特的"细菌-细菌"寄生关系,并可能在牙周炎等口腔疾病中发挥重要作用。作为近年才得以分离培养的新微生物,较少的可培养菌株数量限制了对其寄生行为的深入理解。本研究通过平行诱饵技术分离解糖微小寄生菌,从株水平比较细菌间差异对其寄生行为的影响,并通过测序技术在基因水平上初步探讨菌株间差异对寄生行为的影响。材料和方法:收集志愿者的非刺激性唾液样本（n=16）置于放线菌选择性培养基中厌氧培养,将分离鉴定得到的不同放线菌保存于96孔板中建立宿主库。收集牙周炎患者的非刺激性唾液（n=16）,离心后使用0.45μm滤网过滤得到含解糖微小寄生菌的菌液。将过滤后的菌液分别加入放线菌宿主库孔板中,微需氧条件下培养得到稳定的解糖微小寄生菌-宿主共培养物,达到分离解糖微小寄生菌的目的。观察不同解糖微小寄生菌的生物学特性及其与不同宿主放线菌（n=15）的结合能力,并选取同一菌种不同菌株的解糖微小寄生菌与放线菌宿主进行"生长-抑制"试验,分析菌株间差异对其寄生行为的影响。之后进行全基因组测序并分析不同菌株的基因特点。结果:通过分离鉴定获得76种不同的放线菌种纳入宿主库。采用平行诱饵技术新发现14株解糖微小寄生菌,其中10株属于HMT-352菌种。HMT-352菌种的10个菌株与宿主的结合能力各不相同,"生长-抑制"试验表明各菌株与放线菌的寄生行为也各不相同。测序分析结果提示编码未知蛋白的Gc0000518基因可能在解糖微小寄生菌的寄生行为中发挥关键作用。结论:通过建立放线菌宿主库,使用平行诱饵技术可以高效的分离口腔解糖微小寄生菌。不同菌株表现出不同的宿主结合能力和寄生生长特性,在菌株水平比较解糖微小寄生菌的表型和基因差异更有助于我们进一步探寻寄生行为的内在机制。

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降雨影响散射计测风的初步研究

遥感技术与应用 2005年第1期20卷 157-161页

作者：杨吉龙张雪虎陈秀万柯樱海 daniel Esteban James carswell David JMc Laughlin Paul chang Peter Black Frank Marks 北京大学遥感与地理信息系统研究所 Dept of Electrical &computer NOAA USA

给出了降雨影响c、Ku波段微波散射计测量海面风速的初步结果。研究结果显示,海面风速为25～30m/s时,雨速为15mm/hr的降雨会使这两个波段的微波散射计测量的风速偏低10m/s。

关键词：降雨效应海面风场微波散射计飓风遥感

提供给热带森林管理的地方知识:植物次生化学节律和月亮周期的关系

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AMBIO－人类环境杂志 2002年第6期31卷 485-490页

作者： Kristiina A.Vogt KarenH.Beard Shira Hammann Jennifer O'Hara Palmiotto daniel J.Vogt Frederick N Scatena Brooke P.Hecht 王胜华盛顿大学犹他州立大学于康涅狄格新港耶鲁大学于康涅狄格新港耶鲁森林和环境研究学院森林资源学院宾夕法尼亚州大学耶鲁大学

本研究运用满月时收获非木材林产品的当地实践知识,来证明控制叶片分解速率的化学物质随月亮周期而波动,并且这种现象可能已发展为一种植物—食草动物之间的相互关系。当地知识认为,满月期收获的叶片更为耐用。满月期收获的棕榈叶片具... 详细信息

本研究运用满月时收获非木材林产品的当地实践知识,来证明控制叶片分解速率的化学物质随月亮周期而波动,并且这种现象可能已发展为一种植物—食草动物之间的相互关系。当地知识认为,满月期收获的叶片更为耐用。满月期收获的棕榈叶片具有较高的总碳量、半纤维素和碳化合物,而钙的浓度则较低。这些化学上的变化使棕榈叶在满月收获时对食草动物不太敏感且更为耐用。本研究提出了热带植物减少食叶动物食用及影响衰老叶片的分解速度和耐用性(尤其在满月期)的作用机制。本研究认为森林经营中有必要利用天然的生命周期,并为当地人的收获实践提供了科学依据。

关键词：热带森林植物次生化学节律月亮周期叶片分解速率森林管理森林经营

卫生经济学评价报告标准共识2022(cHEERS 2022):卫生经济学评价报告指导意见更新版

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英国医学杂志中文版 2022年第8期25卷 460-465页

作者： Don Husereau Michael Drummond Federico Augustovski Esther de Bekker-Grob Andrew H Briggs chris carswell Lisa caulley Nathorn chaiyakunapruk Dan Greenberg Elizabeth Loder Josephine Mauskopf c daniel Mullins Stavros Petrou Raoh-Fang Pwu Sophie Staniszewska on behalf of cHEERSISPOR Good Research Practices Task Force 肖月(译) 邱英鹏(译) 史黎炜(译) 张治国(译) 张歆(译) 王海银(译) 翟铁民(译) School of Epidemiology and Public Health University of OttawaOntarioCanada Institute of Health EconomicsAlbertaCanada centre for Health Economics University of YorkYorkUK Health Technology Assessment and Health Economics Department of the Institute for clinical Effectiveness and Health Policy(IEcS-cONIcET) Buenos Aires University of Buenos AiresBuenos Aires CONICET(National Scientific and Technical Research Council)Buenos AiresArgentina Erasmus School of Health Policy&Management Erasmus University RotterdamRotterdamThe Netherlands London School of Hygiene and Tropical Medicine LondonEnglandUK Adis Journals Springer NatureAucklandNew Zealand Department of Otolaryngology-Head&Neck Surgery University of OttawaOntarioCanada Clinical Epidemiology Program and Center for JournalologyOttawa Hospital Research InstituteOntarioCanada Department of EpidemiologyErasmus University Medical Center RotterdamRotterdamThe Netherlands Department of Pharmacotherapy College of PharmacyUniversity of UtahSalt Lake CityUtahUSA Department of Health Policy and Management School of Public HealthFaculty of Health SciencesBen-Gurion University of the NegevBe′er-ShevaIsrael Harvard Medical School BostonMAUSA The BMJLondonUK RTI Health Solutions RTI InternationalResearch Triangle ParkNCUSA School of Pharmacy University of Maryland BaltimoreBaltimoreMDUSA Nuffield Department of Primary care Health Sciences University of OxfordOxfordUK National Hepatitis c Program Office Ministry of Health and WelfareTaipei CityTaiwanChina warwick Research in Nursing University of Warwick Warwick Medical SchoolWarwickUK 不详国家卫生健康委卫生发展研究中心北京100043 国家药品和卫生技术综合评估中心北京00043 英国谢菲尔德大学(The University of Sheffield) 谢菲尔德S102TN 华中科技大学武汉430074 哈尔滨医科大学哈尔滨150086 上海市卫生和健康发展研究中心上海200031

卫生经济学评价是对备选行动方案的成本和结果的比较分析。2013年发表的《卫生经济学评价报告标准共识》(cHEERS)提出了卫生经济学评价的特点,确保结果正确阐释,以支持决策制订。2013版cHEERS旨在指导研究报告撰写者准确报告卫生干预和... 详细信息

卫生经济学评价是对备选行动方案的成本和结果的比较分析。2013年发表的《卫生经济学评价报告标准共识》(cHEERS)提出了卫生经济学评价的特点,确保结果正确阐释,以支持决策制订。2013版cHEERS旨在指导研究报告撰写者准确报告卫生干预和对照措施、背景、过程、结果等评价细节,使审稿人理解文章内容,帮助读者使用研究结果。本版共识将取代2013版共识,其更加适用于各类卫生经济学评价,结合学科发展和方法更新的需要,更注重患者、公众等相关利益方的参与。本共识适用多种个体或人群健康干预措施(简单或复杂)在不同政策场景的应用分析,包括医疗服务、公共卫生、教育、社会服务等。本文概要介绍了cHEERS 2022共识中包含的28个检查项及每一个检查项的相关建议。cHEERS 2022共识主要用于指导研究人员撰写拟投递同行评议学术期刊的卫生经济学评价文章,亦可用于期刊编辑和审稿专家对待发表文章的评价。熟悉了解本共识要求,还有助于研究人员规划评价研究。随着决策透明度要求提高,本共识可支持卫生技术评估机构建立评价报告标准。

关键词：期刊编辑审稿专家检查项学术期刊审稿人公共卫生人群健康评价报告

Antihyperglycemic effects of total ginsenosides from leaves and stem of Panax ginseng

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Acta Pharmacologica Sinica 2005年第9期26卷 1104-1110页

作者： Jing-tian XIE~(1,3) chong-zhi wANG~(1,3) An-bao wANG~(1,3) Jian wU~(1,3) daniel BASILA~(1,3) chun-su YUAN~(1,2,3,4)1 Tang center for Herbal Medicine Research 2 committee on clinical Pharmacology 3 Departments of Anesthesia and critical care,the Pritzker School of Medicine University of chicago,chicago,Illinois 60637,USA Tang center for Herbal Medicine Research the Pritzker School of Medicine University of Chicago Chicago Illinois 60637 USA

Aim:The antihyperglycemic effects of the total ginsenosides in chinese ginseng(TGcG),extracted from leaves and the stem,were evaluated in diabetic c57BL/6Job/ob ***:Animals received daily intraperitoneal injections of TGcG(100 and 200 mg/kg)or oral administration(150 and 300 mg/kg)for 12 *** glucose levels and body weight were measured after fasting the animals for4 *** glucose use was also measured using an intraperitoneal glucosetolerance ***:In the injection group,a high dose of TGcG(200 mg/kg)significantly lowered the fasting blood glucose levels in ob/ob mice on d 12(153±16 mg/dL vs 203±9.8 mg/dL,Pcompared to vehicle-treated group).In theoral group,blood glucose decreased notably with a dose of TGcG(300 mg/kg)ond 12(169.1±12.6 mg/dL vs 211.6±13.8 mg/dL,Pcompared to the vehicle-treated group).Glucose tolerance was also improved markedly in ob/ob ***,a significant reduction in bodyweight(Pwas observed after12 d ofTGcG(300 mg/kg)treatment in mice from the oral ***:Theresults indicated that in a diabetic ob/ob mouse model TGcG was endowed withsignificant anti-hyperglycemic and anti-obesity ***,the totalginsenosides extracted from chinese ginseng leaves and the stem may have somepotential for treating diabetes.

关键词： Panax ginseng total ginsenosides diabetes mellitus hyperglycemia obesity ob/ob mice

MR扩散加权最大信号强度投影技术对乳腺癌X线筛查中可疑病灶的快速和无创鉴定

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国际医学放射学杂志 2016年第3期 299-299页

作者： S.Bickelhaupt F.Laun J.Tesdorff w.Lederer H.daniel A.Stieber 吴龙 Departments of Radiology and Medical Physics in Radiology German Cancer Research Center

摘要目的评价包含最大信号强度投影(MIP)的背景抑制扩散加权成像(DwIBS)以及非增强形态学序列的诊断用简易MRI预测乳腺X线筛查可疑病灶中恶性可能性的能力,并与简易对比增强MRI及全诊断用乳腺MRI方案进行比较。材料与方法本研究为前瞻... 详细信息

摘要目的评价包含最大信号强度投影(MIP)的背景抑制扩散加权成像(DwIBS)以及非增强形态学序列的诊断用简易MRI预测乳腺X线筛查可疑病灶中恶性可能性的能力,并与简易对比增强MRI及全诊断用乳腺MRI方案进行比较。材料与方法本研究为前瞻性研究,获得伦理委员会的批准。在2014年9月—2015年1月期间共纳入乳腺X线筛

关键词： DwIBS 可疑病灶 MR 阳性预测值筛查对比增强乳腺癌乳腺肿瘤信号强度