检索结果-南通市图书馆

Protective and antifungal properties of Nanodisk-Amphotericin B over commercially available Amphotericin B

World Journal of Otorhinolaryngology-Head and Neck Surgery 2017年第1期3卷 -页

作者： Do-Yeon Cho Kyle J.Hoffman Gobind S.Gill Dong-Jin Lim daniel skinner.calvin mackey Steven M.Rowe Bradford A.Woodworth Department of Otolaryngology Head & Neck Surgery University of Alabama at BirminghamBirminghamALUSAGregory Fleming James Cystic Fibrosis Research CenterUniversity of Alabama at BirminghamBirminghamALUSA Department of Otolaryngology Head & Neck Surgery University of Alabama at BirminghamBirminghamALUSA Gregory Fleming James Cystic Fibrosis Research Center University of Alabama at BirminghamBirminghamALUSADepartments of MedicinePediatricsCell Developmental and Integrative BiologyUniversity of Alabama at BirminghamBirminghamALUSA

Objective:Amphotericin B (AMB),a potent antifungal agent,has been employed as topical and systemic therapy for sinonasal fungal infections.A novel formulation of nanodisc (ND) containing super aggregated AMB (ND-AMB) for the treatment of fungal infections has been recently developed to provide greater protection from AMB toxicity than current,clinically approved lipid-based *** objective of the current study was to evaluate the safety and potency of ND-AMB for sinonasal delivery using an in vitro ***:Human sinonasal tissue was harvested during endoscopic sinus surgery and grown at air-liquid interface until *** were exposed to ND-AMB vs AMB and changes in K+ permeability and resistance were measured and recorded via Ussing chamber *** beat frequency (CBF) was analyzed in parallel as well as cytotoxic *** was assessed using real-time PCR measurement of the Aspergillus fumigatus 18S ***:Ussing chamber studies revealed K+ currents that increased rapidly within 30 s of adding AMB (10 μg/mL) to the apical side,indicating apical membranes had become permeable to K+ *** contrast,negligible induction of K+ current was obtained following addition of NDAMB [AMB =(107.7 ± 15.9) μA/cm2 AMB vs ND-AMB =(2.3 ± 0.7) μA/cm2 ND-AMB;P =0.005].ND-AMB also protected nasal epithelial cells from cytotoxicity of AMB (P ＜ 0.05).There was no difference in ciliary beat frequency between the two groups (P =0.96).The expression of *** 18S rRNA with exposure of lower dose of ND-AMB was significantly lower compared to that with AMB (P ＜ 0.05).Conclusions:Data from the present study suggests ND-AMB protects human nasal epithelia membranes from AMB toxicity by protecting against apical cell K+ permeability while maintaining uncompromised antifungal property compared to ***-AMB could provide a novel topical therapy for sinonasal fungal diseases.

关键词： Chronic rhinosinusitis Amphotericin Drug delivery Transepithelial ion transport Sinusitis Sinus epithelium Mucociliary clearance Ciliary beat frequency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：