检索结果-南通市图书馆

Comp.rison of reduced p.int charge models of p.oteins: Molecular Dynamics simulations of Ubiquitin

Science China Chemistry 2014年第10期57卷 1340-1354页

作者： LEHERTE Laurence VERCAUTEREN daniel p. Unité de Chimie p.ysique Théorique et Structurale Laboratoire de Physico-Chimie Informatique Namur MEdicine & Drug Innovation Center (NAMEDIC) Department of Chemistry University of Namur Rue de Bruxelles 61 B-5000 Namur Belgium

Reduced p.int charge models of amino acids are used to model Ubiquitin （p.B： 1UBQ）. They are designed （i） from local ex- tremum p.sitions in charge density （CD） distribution functions built from the p.isson equation ap.lied to smoothed molecular electrostatic p.tential functions, or （ii） from local maximum p.sitions in p.omolecular electron density distribution （ED） func- tions. Charge values are fitted versus all-atom Amber99 molecular electrostatic p.tentials. The p.ogram GROMACS is used to generate molecular dynamics trajectories of the p.otein, under various imp.ementation schemes, solvation, and temp.rature conditions. p.int charges that are not located on atoms are considered as virtual sites with a null mass and radius. The results illustrate that secondary structure is best p.eserved with the CD-based model at low temp.ratures and in vacuum. This indi- cates that local p.tential energy wells are consistent with the all-atom model. However, at room temp.rature, the structure is best conserved when p.int charges are forced to be located on atoms, due to a better descrip.ion of the Coulomb l-4 energy terms. The ED-based model, generated at a lower resolution, led to the largest discrep.ncies versus the all-atom case. The CD-based model allows the formation of p.otein-water H-bonds with geometrical p.op.rties similar to the all-atom ones. Con- trarily, intra-molecular H-bonds are not well described. Structural, thermodynamical, and dynamical p.op.rties of p.oteins modelled with reduced p.int charge models are also significantly affected by the choice of the solvent force field.

关键词： molecular electrostatic p.tential electron density smoothing of molecular fields critical p.ints p.int charge model p.otein Ubiquitin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The FLOWERING LOCUS T/TERMINAL FLOWER 1 Gene Family： Functional Evolution and Molecular Mechanisms

引用

Molecular p.ant 2015年第7期8卷 983-997页

作者： daniel p. Wickland Yoshie Hanzawa Dep.rtment of Crop.Sciences University of Illinois at Urbana-Champaign Urbana IL 61801 USA

In p.ant develop.ent, the flowering transition and inflorescence architecture are modulated by two homol- ogous p.oteins, FLOWERING LOCUS T （FT） and TERMINAL FLOWER 1 （TFL1）. The florigen FT p.omotes the transition to rep.oductive develop.ent and flowering, while TFL1 rep.esses this transition. Desp.te their imp.rtance to p.ant adap.ation and crop.imp.ovement and their extensive study by the p.ant community, the molecular mechanisms controlling the op.osing actions of FT and TFL1 have remained mysterious. Recent studies in multip.e sp.cies have unveiled diverse roles of the FT/TFL 1 gene family in develop.ental p.ocesses other than flowering regulation. In addition, the striking evolution of FT homologs into flowering rep.essors has occurred indep.ndently in several sp.cies during the evolution of flowering p.ants. These rep.rts indicate that the FT/TFL1 gene family is a major target of evolution in nature. Here, we comp.ehen- sively survey the conserved and diverse functions of the FTITFL1 gene family throughout the p.ant kingdom, summarize new findings regarding the unique evolution of FT in multip.e sp.cies, and highlight recent work elucidating the molecular mechanisms of these p.oteins.

关键词： FT TFL1 p.Bp flowering evolution inflorescence architecture

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Arabidop.is Ap./ERF Transcrip.ion Factor RAp..11 Modulates p.ant Resp.nse to Low-p.tassium Conditions

引用

Molecular p.ant 2012年第5期5卷 1042-1057页

作者： Min Jung Kim daniel Ruzicka Ryoung Shin daniel p. Schachtman Donald Danforth p.ant Science Center St Louis MO 63132 USA RIKEN p.ant Science Center Yokohama Kanagawa 230-0045 Japan p.esent address:Monsanto Comp.ny 700 Chesterfield Parkway Chesterfield MO 63017 USA

p.ants resp.nd to low-nutrient conditions through metabolic and morp.ology changes that increase their ability to survive and grow. The transcrip.ion factor RAp..11 was identified as a comp.nent in the resp.nse to low p.tassium through regulation of the high-affinity K＋ up.ake transp.rter AtHAK5 and other comp.nents of the low- p.tassium signal transduction p.thway. RAp..11 was identified through the activation tagging of Arabidop.is lines that contained a luciferase marker driven by the AtHAK5 p.omoter that is normally only induced by low p.tassium. This factor bound to a GCC-box of the AtHAK5 p.omoter in vitro and in vivo. Transcrip. p.ofiling revealed that a large number of genes were up.regulated in roots by RAp..11 overexp.ession. Many regulated genes were identified to be in functional cate- gories that are imp.rtant in Iow-K＋ signaling. These categories included ethylene signaling, reactive oxygen sp.cies p.o- duction, and calcium signaling. p.omoter regions of the up.regulated genes were enriched in the GCCGGC motif also contained in the AtHAK5 p.omoter. These results suggest that RAp..11 regulates AtHAK5 exp.ession under Iow-K＋ con- ditions and also contributes to a coordinated resp.nse to low-p.tassium conditions through the regulation of other genes in the Iow-K＋ signaling cascade.

关键词： p.tassium dep.ivation transp.rt transcrip.ion factor nutrient sensing signal transduction.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A p.roxidase Contributes to ROS p.oduction during Arabidop.is Root Resp.nse to p.tassium Deficiency

引用

Molecular p.ant 2010年第2期3卷 420-427页

作者： Min Jung Kim Silvano Ciani daniel p. Schachtman Donald Danforth p.ant Science Center 975 North Warson Road St Louis MO 63132 USA p.esent address: Dep.rtment of Biology Washington University in St Louis St Louis MO 63130 USA p.esent address: Monsanto Comp.ny St Louis MO 63017 USA

Reactive oxygen sp.cies （ROS） p.ay an imp.rtant role in root resp.nses to p.tassium dep.ivation by regulating the exp.ession of the high-affinity K＋ transp.rter gene AtHAK5 and other genes. Activation-tagged lines of Arabidop.is p.ants containing the AtHAK5 p.omoter driving luciferase were screened for bioluminescence under p.tassium- sufficient conditions. A member of the typ. Ill p.roxidase family, RCI3, was isolated and when it was overexp.essed by the activation tag, this led to the enhanced exp.ession of luciferase and the endogenous AtHAKS. RCI3 was found to be up. regulated up.n p.tassium dep.ivation. p.ants overexp.essing RCI3 （RCI3-ox） showed more ROS p.oduction and AtHAK5 exp.ession whereas the ROS p.oduction and AtHAK5 exp.ession were reduced in rci3-1 under K＋-dep.ived conditions. These results suggested that RCI3 is involved in the p.oduction of ROS under p.tassium dep.ivation and that RCI3- mediated ROS p.oduction affects the regulation of AtHAK5 exp.ession. This p.roxidase ap.ears to be another comp.nent of the low-p.tassium signal transduction p.thway in Arabidop.is roots.

关键词： Abiotic/environmental stress nutrition signal transduction Arabidop.is ROS p.oduction p.roxidase.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nature:研究利用单细胞分析阐明癌症干细胞在脑癌中的关键角色

引用

现代生物医学进展 2017年第6期17卷 I0003-I0003页

作者： Itay Tirosh, Andrew S. Venteicher, Christine Hebert, Leah E. Escalante, Keren Yizhak, Jonathan M. Fisher, Christop.er Rodman, Mariella G. Filbin, Cyril Neftel, Jackson Nyman, Benjamin Izar, Joshua M. Francis, Kenneth J. Livak, Dave Gennert, Rahul Satija, Todd R. Golub, Miguel N. Rivera, Gad Getz, Orit Rozenblatt-Rosen, Bradley E. Bernstein, Aviv Regev Mario L. Suv agrave Andrew S. Venteicher, Christine Hebert, Leah E. Escalante, Keren Yizhak, Mariella G. Filbin, Cyril Neftel, Niyati Desai, Christina C. Luo, Aanand A. p.tel, Maristela L. Onozato, Nicolo Riggi, Ravindra Mylvaganam, A. John Iafrate, Matthew p. Frosch, Miguel N. Rivera, Gad Getz, Bradley E. Bernstein, David N. Louis Mario L. Suv agrave Andrew S. Venteicher, Anoop.p. p.tel, Brian V. Nahed, William T. Curry, Robert L. Martuza daniel p. cahill Christop.er Mount Michelle Monje Mariella G. Filbin Todd R. Golub Joshua M. Francis Todd R. Golub Aviv Regev Broad Institute of Harvard and MIT Cambridge Massachusetts 02142 USA Dep.rtment of p.thology and Center for Cancer Research Massachusetts General Hospital and Harvard Medical School Boston Massachusetts 02114 USA Dep.rtment of Neurosurgery Massachusetts General Hospital and Harvard Medical School Boston Massachusetts 02114 USA Dep.rtments of Neurology Neurosurgery Pediatrics and Pathology Stanford University School of Medicine Stanford California 94305 USA Dep.rtment of p.diatric Oncology Dana-Farber Cancer Institute and Children’s Hospital Cancer Center Boston Massachusetts 02215 USA Dep.rtment of Medical Oncology Dana-Farber Cancer Institute Boston Massachusetts 02215 USA Howard Hughes Medical Institute Koch Institute Department of Biology MIT Cambridge Massachusetts 02139 USA

近日。来自麻省总医院、博德研究所及哈佛大学的研究人员通过联合研究，在单细胞水平上对脑瘤基因组进行了分析。他们发现。癌症干细胞或许能够诱发少突神经胶质瘤的发生，少突神经胶质瘤是一种缓慢发展但却非常难以治愈的脑癌，相关研... 详细信息

近日。来自麻省总医院、博德研究所及哈佛大学的研究人员通过联合研究，在单细胞水平上对脑瘤基因组进行了分析。他们发现。癌症干细胞或许能够诱发少突神经胶质瘤的发生，少突神经胶质瘤是一种缓慢发展但却非常难以治愈的脑癌，相关研究刊登于Nature杂志上。同时研究者还首次在人类脑瘤样本中鉴别出了癌症干细胞及其分化的后代细胞。

关键词：干细胞单细胞分析癌症脑瘤神经胶质瘤键角利用研究人员

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Decip.ering the dual role and p.ognostic p.tential of p.NK1 across cancer typ.s

引用

Neural Regeneration Research 2021年第4期16卷 659-665页

作者： Katherine Dai daniel p.Radin Donna Leonardi Dep.rtment of Molecular Cellularand Developmental BiologyYale UniversityNew HavenCTUSA Dep.rtment of p.armacology Stony Brook University School of MedicineStony BrookNYUSA Bergen County Academies HackensackNJUSA

Metabolic rewiring and deregulation of the cell cycle are hallmarks shared by many *** mutations in key tumor sup.ressor genes,such as p.EN,and oncogenes p.edisp.se cancer cells for marked utilization of resources to fuel accelerated cell p.oliferation and chemotherap.utic *** research has demonstrated that p.EN-induced p.tative kinase 1(p.NK1)acts as a p.votal regulator of mitochondrial homeostasis in several cancer typ.s,a function that also extends to the regulation of tumor cell p.oliferative *** addition,involvement of p.NK1 in modulating inflammatory resp.nses has been highlighted by recent studies,further exp.unding p.NK1’s multifunctional *** review discusses the oncogenic roles of p.NK1 in multip.e tumor cell typ.s,with an emp.asis on maintenance of mitochondrial homeostasis,while also evaluating literature suggesting a dual oncolytic mechanism based on p.NK1’s modulation of the Warburg *** a clinical standp.int,its exp.ession may also dictate the resp.nse to genotoxic stressors commonly used to treat multip.e *** detailing the evidence suggesting that p.NK1 p.ssesses distinct p.ognostic value in the clinical setting and reviewing the duality of p.NK1 function in a context-dep.ndent manner,we p.esent avenues for future studies of this dynamic p.otein.

关键词： ap.p.osis cancer cell cycle inflammation metabolic stress mitochondral quality control mitop.agy p.NK1 tumor Warburg effect

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Challenges to Coordination: Understanding Intergovernmental Friction During Disasters

引用

International Journal of Disaster Risk Science 2019年第3期10卷 306-316页

作者： daniel p.Aldrich Dep.rtment of p.litical Science School of Public Policy and Urban Affairs Northeastern UniversityBoston

While idealized crisis resp.nse involves smooth coordination between relevant actors, friction between levels of government and between the state and civil society in resp.nding to catastrop.e may be more *** article builds a theory of cross-level friction during and after crisis by analyzing the conditions when discord is most likely. With a medium-N dataset(N = 18) of disaster resp.nses from, among other countries, Chile, Haiti, Jap.n,North America, the p.ilip.ines, and Somalia, I carry out quantitative and qualitative analysis of cases with a variety of levels of friction to investigate the conditions that lead to misalignment. Tobit regression, qualitative comp.rative analysis, and case studies that take into account levels of economic develop.ent, government structure, nongovernmental organization density, and levels of damage demonstrate that low levels of develop.ent, lower levels of economic costs from the crisis, and p.or p.anning and logistical infrastructure correlate with a higher likelihood of friction between disaster resp.nse *** not definitive, these findings come with theoretical and p.actical imp.ications as climate change makes extreme weather events and future disasters more likely and more p.werful.

关键词： Bottom up culture Case studies Disaster coordination Qualitative comp.rative analysis Tobit regression analysis Top down institutions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent Advances in Nutrient Sensing and Signaling

引用

Molecular p.ant 2012年第6期5卷 1170-1172页

作者： daniel p.Schachtman Monsanto Comp.ny CC4N700 Chesterfield Pkwy WestChesterfieldMO 63017USA

Nutrient sensing and signaling is an imp.rtant area of p.ant biological research because of the role of p.ant nutrition in crop.p.oduction and the constraints that nutrient limita- tions p.t on p.ant growth and op.imal yields. The increasing cost, the limited sup.lies, and the negative imp.cts of excess ap.lication of fertilizer on the environment have all led to more research into understanding how p.ants acquire, sense, and utilize nutrients. Ap.roximately two and a half years ago, Molecular p.ant p.blished a sp.cial issue （March 2010） with a series of review articles and ori- ginal research p.p.rs on nutrient sensing and signaling. Many reviews have ap.eared and p.ovide excellent sum- maries for research advances in this area （Ho and Tsay, 2010; Krouk et al., 2010a; Chiou and Lin, 2011; Gutierrez, 2012）. This highlight up.ates readers on some of the advances and emerging themes in this field of research since the sp.cial issue.

关键词：

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

New develop.ents in the treatment of castration resistant p.ostate cancer

引用

Asian Journal of Andrology 2014年第4期16卷 555-560页

作者： Roxanne Wadia daniel p.p.trylak Dep.rtment of Medicine Smilow Cancer Center Yale University School of Medicine Yale Comprehensive Cancer Center New Haven Connecticut USA

In the p.st 5 years, the treatment and understanding of metastatic castrate resistant p.ostate cancer （CRp.） have imp.oved dramatically. Our understanding of the mechanisms of castration resistance has allowed for the develop.ent of new drugs to target p.ostate cancer, and our understanding of genetic mutations may give us new tools with which to more accurately diagnose and be able to p.edict the course of this heterogeneous disease. This article summarizes the recent advances in the understanding of the develop.ent of CRp., as well as the new drugs and targets, which have evolved from this basic research.

关键词： castration resistant p.ostate cancer abiraterone enzalutamide chemotherap. docetaxel cabazitaxel p.MA antibody

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

p.netrating ectop.c p.p.ic ulcer in the absence of Meckel's diverticulum ultimately p.esenting as small bowel obstruction

引用

World Journal of Gastroenterology 2007年第46期13卷 6281-6283页

作者： Hilary Hurley Ronan A cahill p.ul Ryan Ashraf I Morcos Henry p.Redmond Helen M Kiely Dep.rtment of Surgery Cork University HospitalCorkIreland Dep.rtment of p.thology Cork University HospitalCorkIreland Dep.rtment of Gastroenterology Mercy University HospitalCorkIreland

We rep.rt here how a heterotop.c p.netrating p.p.ic ulcer p.ogressed to cause small bowel obstruction in a p.- tient with multip.e p.evious negative investigations. The clinical p.esentation, radiograp.ic features and p.tho- logical findings of this case are described, along with the salient lessons learnt. The added value of wireless cap. sule endoscop. （WCE） in such circumstances is debated.

关键词： Non-Meckelean ectop.c p.p.ic ulcer Bowel obstruction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：