检索结果-南通市图书馆

A REF6-dependent H3K27me3-depleted state facilitates gene activation during germination in Arabidopsis

Journal of Genetics and Genomics 2023年第3期50卷 178-191页

作者： Jie Pan Huairen Zhang Zhenping Zhan Ting Zhao danhua jiang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Seed germination is a critical developmental switch from a quiescent state to active growth,which involves extensive changes in metabolism,gene expression,and cellular identity.However,our understanding of epigenetic and transcriptional reprogramming during this process is limited.The histone H3 lysine 27 trimethylation(H3K27me3)plays a key role in regulating gene repression and cell fate specification.Here,we profile H3K27me3 dynamics and dissect the function of H3K27 demethylation during germination in Arabidopsis.Our temporal genome-wide profiling of H3K27me3 and transcription reveals delayed H3K27me3reprogramming compared with transcriptomic changes during germination,with H3K27me3 changes mainly occurring when the embryo is entering into vegetative development.RELATIVE OF EARLY FLOWERING 6(REF6)-mediated H3K27 demethylation is necessary for robust germination but does not significantly contribute to H3K27me3 dynamics during germination,but rather stably establishes an H3K27me3-depleted state that facilitates the activation of hormone-related and expansin-coding genes important for germination.We also show that the REF6 chromatin occupancy is gradually established during germination to counteract increased Polycomb repressive complex 2(PRC2).Our study provides key insights into the H3K27me3 dynamics during germination and suggests the function of H3K27me3 in facilitating cell fate switch.Furthermore,we reveal the importance of H3K27 demethylation-established transcriptional competence in gene activation during germination and likely other developmental processes.

关键词： H3K27me3 Epigenetic reprogramming H3K27 demethylation REF6 Germination Arabidopsis thaliana

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Histone modifications and their regulatory roles in plant development and environmental memory

引用

Journal of Genetics and Genomics 2019年第10期46卷 467-476页

作者： Ting Zhao Zhenping Zhan danhua jiang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100101China

Plants grow in dynamic environments where they receive diverse environmental signals.Swift and precise control of gene expression is essential for plants to align their development and metabolism with fluctuating surroundings.Modifications on histones serve as histone code" to specify chromatin and gene activities.Different modifications execute distinct functions on the chromatin,promoting either active transcription or gene silencing.Histone writers,erasers,and readers mediate the regulation of histone modifications by catalyzing,removing,and recognizing modifications,respectively.Growing evidence indicates the important function of histone modifications in plant development and environmental responses.Histone modifications also serve as environmental memory for plants to adapt to environmental changes.Here we review recent progress on the regulation of histone modifications in plants,the impact of histone modifications on environment-controlled developmental transitions including germination and flowering,and the role of histone modifications in environmental memory.

关键词： Histone modification Transcription Germination Flowering Plant-environmental response Environmental memory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spatial dynamics of a nonlocal delayed unstirred chemostat model with periodic input

引用

International Journal of Biomathematics 2019年第6期12卷 55-72页

作者： Wu-Jun Pu danhua jiang Ya Wang Zhanbing Bai Department of Mathematics Longnan Teachers College LongnanGansu 742500P.R.China School of Mathematics and Statistics Lanzhou University LanzhouGansu 730000P.R.China College of Mathematics and System Science Shandong University of Science and Technology Qingdao Shandong 266590P.R.China

In this paper,a nonlocal delayed chernostat model of a single species feeding on a periodically varying input nutrient is proposed.By the theory of semigroup,the existence and uniqueness of solution of the system are obtained.Furthermore,we investigate a threshold result on the global dynamics,and the uniform persistence of the system is established.

关键词： Chernostat nonlocal delay uniform persistence periodic solution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

胸腔镜膈肌折叠术治疗小儿膈膨升2种不同缝线缝合打结法的比较

引用

中国微创外科杂志 2024年第1期24卷 34-40页

作者：欧作强蒋丹华黄高杰李永明黄名曾甜广西壮族自治区妇幼保健院新生儿外科广西儿科疾病临床研究中心南宁530003

目的探讨改良Prolene线双头针“U”型缝合联合腔外腔内结合打结法在胸腔镜膈肌折叠术治疗小儿膈膨升中应用的可行性、安全性及手术效果。方法回顾性分析我院2010年5月~2022年5月70例膈膨升行胸腔镜膈肌折叠术的临床资料,根据缝合打结方... 详细信息

目的探讨改良Prolene线双头针“U”型缝合联合腔外腔内结合打结法在胸腔镜膈肌折叠术治疗小儿膈膨升中应用的可行性、安全性及手术效果。方法回顾性分析我院2010年5月~2022年5月70例膈膨升行胸腔镜膈肌折叠术的临床资料,根据缝合打结方法的不同分为改良组(改良Prolene线双头针“U”型缝合联合腔外腔内结合打结法,n=30)和常规组(腔内缝合打结法,n=40),比较2组患儿围术期指标及有无线结松开或膈膨升复发等。结果70例手术均顺利完成,无中转开放手术。改良组手术时间明显短于常规组[(35.3±7.4)min vs.(64.7±10.8)min,t=13.521,P=0.000]。2组术中出血量、留置胸腔引流管时间、术后住院时间、术前术中和术后动脉血气pH值、PO_(2)、PCO_(2)和术后术侧膈肌轻微上升等差异无统计学意义(P>0.05)。70例术后随访6~24个月,中位随访时间12个月,均无线结松开和膈膨升复发,无死亡。结论胸腔镜膈肌折叠术治疗小儿膈膨升采用改良Prolene线双头针“U”型缝合联合腔外腔内结合打结法安全可行、疗效确切,且简单易操作,具有一定腔镜手术基础的医生可快速掌握,适宜有条件的医院推广开展。

关键词：腔内缝合打结法胸腔镜膈肌折叠术小儿膈膨升

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2

引用

Molecular Plant 2018年第8期11卷 1038-1052页

作者： Haifeng Wang danhua jiang Elin Axelsson Zdravko J. Lorkovic Sean Montgomery Sarah Holec Bas J.G.E. Pieters Abbas H.K. AI Temimi Jasmin Mecinovic Frederic Berger Gregor Mendel Institute (GMI) Austrian Academy of Sciences Vienna Biccenter (VBC) Dr. Bohr-Gasse 3 1030 Vienna Austria Temasek Lifesciences Laboratory 1 Research Link Nationat University of Singapore 117604 Singapore Singapore Department of Biological Sciences National University of Singapore 14 Science Drive 4 117543 Singapore Singapore Institute for Molecules and Materials Radboud University Heyendaalseweg 135 6525 AJ Nijmegen the Netherlands

Heterochromatin Protein 1 （HP1） is a major regulator of chromatin structure and function. In animals, the network of proteins interacting with HP1 is mainly associated with constitutive heterochromatin marked by H3K9me3. HP1 physically interacts with the putative ortholog of the SNF2 chromatin remodeler ATRX, which controls deposition of histone variant H3.3 in mammals. In this study, we show that the Arabidopsis thaliana ortholog of ATRX participates in H3.3 deposition and possesses specific conserved domains in plants. We found that plant Like HP1 （LHP1） protein interacts with ATRX through domains that evolved specifically in land plant ancestors. Loss of ATRX function in Arabidopsis affects the expression of a limited subset of genes controlled by PRC2 （POLYCOMB REPRESSIVE COMPLEX 2）, including the flowering time regulator FLC. The function of ATRX in regulation of flowering time requires novel LHPl-interacting domain and ATPase activity of the ATRX SNF2 helicase domain. Taken together, these results suggest that distinct evolutionary pathways led to the interaction between ATRX and HP1 in mammals and its counterpart LHP1 in plants, resulting in distinct modes of transcriptional regulation.

关键词： ATRX LHPI1 H3 variants Chromatin Histone Evolution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

SARS-CoV-2 mRNA疫苗通用体外生物学活性检测方法的建立及验证

引用

中国生物制品学杂志 2024年第3期37卷 322-328页

作者：王新竹赵丹华苏歧刘欣玉姜崴叶强中国食品药品检定研究院北京102629 长春生物制品研究所有限责任公司吉林长春130012

目的建立通用稳定的SARS-CoV-2 mRNA疫苗的体外生物学活性检测方法,并对方法进行验证,以期用于SARS-CoV-2 mRNA疫苗的质量控制。方法筛选能够对SARS-CoV-2各变异株S蛋白均能较好结合的ELISA试剂盒及转染细胞、细胞铺板浓度、转染剂量,建... 详细信息

目的建立通用稳定的SARS-CoV-2 mRNA疫苗的体外生物学活性检测方法,并对方法进行验证,以期用于SARS-CoV-2 mRNA疫苗的质量控制。方法筛选能够对SARS-CoV-2各变异株S蛋白均能较好结合的ELISA试剂盒及转染细胞、细胞铺板浓度、转染剂量,建立SARS-CoV-2 mRNA疫苗体外生物学活性检测方法,并进行验证。结果筛选到1种对各变异株S蛋白均能良好结合的试剂盒,并确定了转染细胞为HEK293T、细胞铺板浓度为2.5×10^(5)个/mL、转染剂量为6孔板4μg/孔,建立了通用稳定的SARS-CoV-2 mRNA疫苗体外生物学活性检测方法。验证结果显示,该方法能满足检定需求。结论建立的SARS-CoV-2 mRNA疫苗体外生物学活性检测方法相对准确度、线性、中间精密度、范围良好,可用于SARS-CoV-2 mRNA疫苗的质量控制。

关键词： SARS-CoV-2 mRNA疫苗体外生物学活性酶联免疫吸附测定

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microbial community structure analyses and cultivable denitrifier isolation of Myriophyllum aquaticum constructed wetland under low C/N ratio

引用

Journal of Environmental Sciences 2023年第5期127卷 30-41页

作者： Jialiang Zuo Lina Xu Jianlin Guo Shengjun Xu Shuanglong Ma Cancan jiang Dongmin Yang danhua Wang Xuliang Zhuang Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Sino-Danish Center University of Chinese Academy of SciencesBeijing 101408China Institute of Tibetan Plateau Research Chinese Academy of SciencesBeijing 100101China College of Resources and Environmental Sciences Henan Agricultural UniversityZhengzhou 450002China Ningxia Zhongke Jingke Testing Technology Company Yinchuan 750000China

With the rapid expansion of livestock production,the amount of livestock wastewater accumulated rapidly.Lack of biodegradable organic matter makes denitrification of livestock wastewater after anaerobic digestion more difficult.In this study,Myriophyllum aquaticum constructed wetlands(CWs)with efficient nitrogen removal performance were established under different carbon/nitrogen(C/N)ratios.Analysis of community composition reveals the change of M.aquaticum CWs in microbial community structure with C/N ratios.The proportion of Proteobacteria which is one of the dominant phyla among denitrifier communities increased significantly under low C/N ratio conditions.Besides,to obtain cultivable denitrifier that could be added into CWs in situ,33 strains belonging to phylum Proteobacteria were isolated from efficient M.aquaticum CWs,while the best-performing denitrification strain M3-1 was identified as Bacillus velezensis JT3-1(GenBank No.CP032506.1).Redundancy analysis and quadratic models showed that C/N ratio had significant effects on disposal of nitrate(NO_(3)^(−)-N)and the strains isolated could perform well in denitrification when C/N ratio is relatively low.In addition,they have relatively wide ranges of carbon sources,temperature and a high NO_(3)^(−)removal rate of 9.12 mg/(L·hr)at elevated concentrations of 800 mg/L nitrate.Thus,strains isolated from M.aquaticum CWs with low C/N ratio have a practical application value in the treatment of nitrate-containing wastewater.These denitrifying bacteria could be added to CWs to enhance nitrogen removal efficiency of CWs for livestock wastewater with low C/N ratio in the future.

关键词： Myriophyllum aquaticum constructed wetlands Denitrification Microbial community structure Bacillus velezensis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

上海市社区老年人家庭功能与健康自我管理的相关性研究

引用

中国社会医学杂志 2024年第3期41卷 340-345页

作者：杜荣高晶蓉江佳艳戴丹华李静李扬张斌赵琦复旦大学公共卫生学院国家卫生健康委员会卫生技术评估重点实验室(复旦大学)上海200032 上海市老龄事业发展促进中心上海200062 上海市闵行区七宝社区卫生服务中心上海201101 上海市松江区中山街道社区卫生服务中心上海201613 上海市黄浦区豫园街道社区卫生服务中心上海200010 上海市虹口区嘉兴路街道社区卫生服务中心上海200082

目的了解上海市社区老年人家庭功能及其健康自我管理水平,并探讨两者之间的相关性。方法采用便利抽样,对2021年11-12月在上海市社区卫生服务中心和站点就诊的1 685名老年人进行面对面问卷调查,调查问卷包括基本人口学特征、成年人健... 详细信息

目的了解上海市社区老年人家庭功能及其健康自我管理水平,并探讨两者之间的相关性。方法采用便利抽样,对2021年11-12月在上海市社区卫生服务中心和站点就诊的1 685名老年人进行面对面问卷调查,调查问卷包括基本人口学特征、成年人健康自我管理能力和家庭功能量表。结果调查对象健康自我管理总分为(150.52±24.28)分,其中行为、环境及认知维度的得分分别为(49.97±11.49)分、(38.75±8.32)分和(61.85±8.46)分。731人健康自我管理总体评价较高,占43.4%。与子女经常交流(OR=1.92,95%CI=1.20~3.06)、家庭功能良好(OR=2.42,95%CI=1.85~3.17)、家庭中等收入水平(OR=1.72,95%CI=1.29~2.29)及高收入水平(OR=1.65,95%CI=1.20~2.28)、自评对生活满意(OR=1.31,95%CI=1.03~1.67)等因素有利于老年人的健康自我管理。老年人总体认知水平优于行为能力。结论良好的家庭关系与氛围有利于提升老年人健康自我管理水平,应关注老年人认知与行为能力的鸿沟,在常规健康知识宣讲的基础上增加良好行为的引导与评价。

关键词：老年人家庭功能健康自我管理

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The INO80 chromatin remodeling complex promotes thermo-morphogenesis by connecting H2A.Z eviction and active transcription in Arabidopsis.

引用

Molecular Plant 2021年第11期14卷 1799-1813页

作者： Xue Mande Zhang Huairen Zhao Fengyue Zhao Ting Li Hui jiang danhua State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina

Global warming imposes a major threat to plant growth and crop production. In some plants including Arabidopsis thaliana, elevated temperatures induce a series of morphological and developmental adjustments, termed thermomorphogenesis to facilitate plant cooling under high-temperature conditions. Plant thermal response is suppressed by histone variant H2A.Z. At warm temperatures, H2A.Z is evicted from nucleosomes at thermo-responsive genes, resulting in their expression changes. However, the mechanisms that regulate H2A.Z eviction and subsequent transcription changes are largely unknown. Here, we show that the INO80 chromatin remodeling complex (INO80-C) promotes thermomorphogenesis and activates the expression of thermo-responsive and auxin-related genes. INO80-C associates with PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), a potent regulator in thermomorphogenesis, and mediates temperature-induced H2A.Z eviction at PIF4 targets. Moreover, INO80-C directly interacts with COMPASS-like and transcription elongation factors to promote active histone modification Histone H3 lysine 4 trimethylation (H3K4me3) and RNA Polymerase II (RNA Pol II) elongation, leading to the thermal induction of transcription. Notably, transcription elongation factors SPT4 and SPT5 are required for the H2A.Z eviction at PIF4 targets, suggesting the cooperation of INO80-C and transcription elongation in H2A.Z removal. Our results demonstrate that the (PIF4)-(INO80-C)-(COMPASS-like)-(transcription elongator) module controls plant thermal response, and establish a link between H2A.Z eviction and active transcription.

关键词： Arabidopsis H2A.Z INO80 PIF4 thermomorphogenesis transcription elongation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Improved accuracy of superpixel segmentation by region merging method

引用

Frontiers of Optoelectronics 2016年第4期9卷 633-639页

作者： Song ZHU danhua CAO Yubin WU Shixiong jiang School of Optical and Electronic Information Huazhong University of Science and Technology Wuhan 430074 China

Superpixel as an important pre-processing technique has been successfully used in many vision applications. In this paper, we proposed a region merging method to improve superpixel segmentation accuracy with low computational cost. We first segmented the image into many accurate small regions, and then progressively agglomerated them until the desired region number was reached. The region merging weight was derived from a novel energy function, which encourages the superpixel with color consistency and similar size. Experimental results on the Berkeley BSDS500 data set showed that our region merging method can significantly improve the accuracy of superpixel segmentation. Moreover, the region merging method only need 50ms to process a 481x321 image on a single Intel i3 CPU at 2.5 GHz.

关键词： image processing image segmentation super-pixels region merging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：