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酸法和酶法提取对中华草龟皮胶原蛋白结构和理化特性的影响

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食品工业科技 2025年第1期46卷 79-86页

作者：虞丹丹魏文志关利陈文卓曹晖扬州大学旅游烹饪学院江苏扬州225127 扬州大学中餐非遗技艺传承文化和旅游部重点实验室江苏扬州225127 扬州大学动物科学与技术学院江苏扬州225001

本研究以中华草龟皮胶原蛋白为研究对象,探究不同提取方法对其结构和理化特性的影响。分别采用酸法和酶法对中华草龟皮胶原蛋白进行提取,并运用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、高效液相色谱法、圆二色光谱法、差式扫描量热、扫描... 详细信息

本研究以中华草龟皮胶原蛋白为研究对象,探究不同提取方法对其结构和理化特性的影响。分别采用酸法和酶法对中华草龟皮胶原蛋白进行提取,并运用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、高效液相色谱法、圆二色光谱法、差式扫描量热、扫描电镜、溶解性、感官评价及色度等分析酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC)的组成、结构以及理化特性。结果表明,ASC和PSC的提取率分别为24.50%±0.44%和51.68%±0.06%,酶法提取胶原蛋白的提取率显著高于酸法(P<0.05)。ASC和PSC主要由α1和α2链组成,均为I型胶原蛋白。ASC和PSC中甘氨酸的含量最多,分别是370.17和387.75个残基/1000个氨基酸残基。圆二色光谱表明这两种胶原蛋白二级结构相似,均具有胶原蛋白的特征峰。ASC和PSC的变性温度分别为27.21±0.28℃和28.37±0.23℃。扫描电镜结果表明,ASC具有无序的纤维结构,而PSC的微观结构更为规则。ASC的溶解度整体略低于PSC,PSC的感官品质和色度优于ASC。

关键词：中华草龟皮胶原蛋白酸法提取酶法提取结构理化特性

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Reliable Passive Control for a class of delayed singular systems with random failures

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Reliable Passive Control for a class of delayed singular sys...

What makes Haloxylon persicum grow on sand dunes while H. ammodendron grow on interdune lowlands: a proof from reciprocal transplant experiments

第25届中国控制与决策会议

作者： dandan yu Xian Zhang Xue Lin School of Mathematical Science Heilongjiang Univ.

ISBN: (纸本)9781467355322

In order to remain stability of systems in the case of stochastic actuator faults, the problem of reliable passive control for a class of delayed linear singular systems with actutor stochastic faults is investigated in this paper. The criteria on the existence of reliable controller and the design method of state-feedback controller are derived, under which the resultant closed-loop systems are admissible and passive with desired dissipation by using Lyapunov stability theory, LMI technique and cone complementarity linearization algorithm. Finally, an example and their simulations are presented to illustrate the effectiveness of the approach proposed in this paper.

关键词： reliable passive control random failure linear matrix inequality (LMI) cone complementarity lineariza- tion(CCL)

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Journal of Arid Land 2014年第5期6卷 581-591页

作者： GuiQing XU dandan yu JiangBo XIE LiSong TANG Yan LI State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of Sciences

Determining the mechanisms underlying the spatial distribution of plant species is one of the central themes in biogeography and ecology. However, we are still far from gaining a full understanding of the autecological processes needed to unravel species distribution patterns. In the current study, by comparing seedling recruitment, seedling morphological performance and biomass allocation of two Haloxylon species, we try to identify the causes of the dune/interdune distribution pattern of these two species. Our results show the soil on the dune had less nutrients but was less saline than that of the interdune; with prolonged summer drought, soil water availability was lower on the dune than on the interdune. Both species had higher densities of seedlings at every stage of recruitment in their native habitat than the adjacent habitat. The contrasting different adaptation to nutrients, salinity and soil water conditions in the seedling recruitment stage strongly determined the distribution patterns of the two species on the dune/interdune. Haloxylon persicum on the dunes had lower total dry biomass, shoot and root dry biomass, but allocated a higher percentage of its biomass to roots and possessed a higher specific root length and specific root area by phenotypic traits specialization than that of Haloxylon ammodendron on the interdune. All of these allowed H. persicum to be more adapted to water stress and nutrient shortage. The differences in morphology and allocation facilitated the ability of these two species to persist in their own environments.

关键词： Haloxylon seedling recruitment root distribution biomass allocation species distribution

Evolutionary selection on MDA5 and LGP2 in the chicken preserves antiviral competence in the absence of RIG-I

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Journal of Genetics and Genomics 2019年第10期46卷 499-503页

作者： Ling Xu dandan yu yu Fan Yi-Ping Liu Yong-Gang Yao Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&yunnan Province Kunming Institute of Zoology Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Kunming College of Life Science University of Chinese Academy of Sciences KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of Sciences

The pattern recognition receptors (PRRs) act as the first line of defense against pathogens and are common molecular targets in the conflict between viruses and their host (Tan et al.,2018).The RIG-I-like receptors (RLRs),including RIG-I (Yoneyama et al.,2004),MDA5 (Andrejeva et al.,2004),and LGP2 (Saito et al.,2007),are PRRs and reside in the *** proteins recognize the RNA structures of different viral RNA *** RLRs have an intermediate RNA helicase domain that catalyzes ATP hydrolysis and a C-terminal domain (CTD).

关键词： MDA5 hydrolysis absence

A high-concentrated and nonflammable electrolyte for potassium ion-based dual-graphite batteries

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Nano Research 2023年第5期16卷 6353-6360页

作者： Kexin Li Guiyou Ma dandan yu Wen Luo Jiaxin Li Laishun Qin yuexiang Huang Da Chen College of Materials and Chemistry China Jiliang UniversityHangzhou 310018China Liangxin College China Jiliang UniversityHangzhou 310018China

Potassium ion-based dual-graphite batteries(KDGBs)emerge as promising devices for large-scale applications due to their high voltage,low cost,environmental ***,conventional KPF6/carbonate-based electrolytes suffer from severe oxidation decomposition,low concentration,flammability,which limit the capacity and cyclability of ***,a nonflammable potassium bis(fluorosulfonyl)imide/triethyl phosphate(KFSI/TEP)electrolyte was designed for *** the salt-to-solvent molar ratio increases to 1:1.3,graphite cathode operated at the cut-off potential of 5.2 V exhibits much enhanced capacity,excellent rate capability(26.4 mAh∙g^(−1) at 1.0 A∙g^(−1)),superior cyclability with 98%capacity retention after 350 *** compounds-rich electrode/electrolyte interphase layers derived from the preferential decomposition of FSI−anions ensure good compatibility of the 1:1.3 KFSI/TEP electrolyte with K metal and graphite *** on this electrolyte,asassembled KDGBs show high operation voltage of 4.3 V and good cycling *** work provides feasibility for developing long-life and safe-operation DGBs.

关键词： dual-ion batteries graphite high voltage high-concentrated electrolytes phosphates

Cannabidiol prevents depressive-like behaviors through the modulation of neural stem cell differentiation

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Frontiers of Medicine 2022年第2期16卷 227-239页

作者： Ming Hou Suji Wang dandan yu Xinyi Lu Xiansen Zhao Zhangpeng Chen Chao Yan State Key Laboratory of Pharmaceutical Biotechnology School of Life SciencesNanjing UniversityNanjing 210023China Institute of Artificial Intelligence Biomedicine Nanjing UniversityNanjing 210023China Chemistry and Biomedicine Innovation Center Nanjing UniversityNanjing 210023China Engineering Research Center of Protein and Peptide Medicine Ministry of EducationNanjing 210023China

Chronic stress impairs radial neural stem cell(rNSC)differentiation and adult hippocampal neurogenesis(AHN),whereas promoting AHN can increase stress resilience against ***,investigating the mechanism of neural differentiation and AHN is of great importance for developing antidepressant *** nonpsychoactive phytocannabinoid cannabidiol(CBD)has been shown to be effective against ***,whether CBD can modulate rNSC differentiation and hippocampal neurogenesis is ***,by using the chronic restraint stress(CRS)mouse model,we showed that hippocampal rNSCs mostly differentiated into astrocytes under stress ***,transcriptome analysis revealed that the FoxO signaling pathway was involved in the regulation of this *** administration of CBD rescued depressive-like symptoms in CRS mice and prevented rNSCs overactivation and differentiation into astrocyte,which was partly mediated by the modulation of the FoxO signaling *** results revealed a previously unknown neural mechanism for neural differentiation and AHN in depression and provided mechanistic insights into the antidepressive effects of CBD.

关键词： cannabidiol depression radial neural stem cells neurogenesis

Interlayer and doping engineering in partially graphitic hollow carbon nanospheres for fast sodium and potassium storage

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Chinese Chemical Letters 2023年第2期34卷 582-587页

作者： Dongfei Sun Sen Lin dandan yu Zijuan Wang Fangfang Deng Xiaozhong Zhou Guofu Ma Ziqiang Lei Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education Key Laboratory of Eco-environmental Polymer Materials of Gansu ProvinceCollege of Chemistry and Chemical EngineeringNorthwest Normal UniversityLanzhou 730070China

Constructing anodes with fast ions/electrons transfer paths is an effective strategy to achieve high-performance sodium ion batteries(SIBs)/potassium ion batteries(PIBs). Amorphous carbon is a promising candidate anode for SIBs/PIBs owing to its disordered carbon layers, abundant defects/pores, and lowcost resources. However, the larger radius of Na^(+)/K^(+) leading to depressed kinetics and poor cycling performance, impeding their further applications. Herein, we propose an efficient strategy to construct of nitrogen, sulfur co-doped hollow carbon nanospheres(NS-HCS) involving an in situ growth of polydopamine on nano-Ni(OH)2template with subsequent sulfur doping process. During the formation process, the produced Ni nanospheres play as the hard template and catalyst for the formation of hollow carbon nanosphere with partially graphite microcrystalline structure, while the sulfur doping process can enlarge the interlayer space and create more defects on the surface of carbon nanospheres, thus synchronous improve the Na^(+)/K^(+) insertion and adsorption ability in NS-HCS. With the synergistic control of the enlarged interlayer spacing, high content of pyridinic N/pyrrolic N and graphitization, a hybrid storage mechanism facilitates the transport kinetics and endows the NS-HCS electrode with high capacities and good cycling stability in SIBs and PIB. Benefit from the multiple effects, NS-HCS exhibits the improved capacity of 274.8 m Ah/g at 0.1 A/g and excellent cycling stability of 149.5 m Ah/g after 5000 cycles at2.5A/g in SIBs, as well as good potassium ion storage behavior with a high capacity retention of 76.5%after 700 cycles at 1.0 A/g, demonstrating the potential applications of NS-HCS for high-performance SIBs and PIBs.

关键词： Heteroatom doping Hollow carbon nanosphere Long cycle life Hybrid storage mechanism Sodium ion batteries Potassium ion batteries

Inhibition of PI3K/Akt/m TOR signaling pathway enhances the sensitivity of the SKOV3/DDP ovarian cancer cell line to cisplatin in vitro

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Chinese Journal of Cancer Research 2014年第5期26卷 564-572页

作者： yunlang Cai Xiaoqiang Tan Jun Liu Yang Shen Di Wu Mulan Ren Peilin Huang dandan yu Department of Obstetrics & Gynecology Zhongda HospitalSoutheast University Medical College Southeast University

The activation of the PI3K/AKT/m TOR pathway plays a key role in ovarian cancer tumorigenesis, progression and chemotherapy resistance. This study aimed to explore the possible mechanism that PI-103, a dual inhibitor of phosphatidylinositide 3-kinase and m TOR, enhances the sensitivity of SKOV3/DDP ovarian cancer cell line to cisplatin chemotherapy. The results showed that PI-103 could significantly increase the sensitivity of SKVO3/DDP cells to cisplatin through inhibiting the activation of PI3K/Akt/m TOR signaling pathway and inducing cell cycle arrest and apoptosis.

关键词： PI3K/AKT/m TOR cisplatin ovarian cancer

Anodes for low-temperature rechargeable batteries

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eScience 2024年第5期4卷 70-90页

作者： Jiawei Wang dandan yu Xinyu Sun Hua Wang Jinghong Li School of Chemistry Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of EducationBeihang UniversityBeijing 100191China Department of Chemistry Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing 100084China School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China

Rechargeable alkali metal ion(Li^(+),Na^(+),K^(+))batteries have shown great success in room-temperature energy ***,their low-temperature(subzero temperature)applications are still severely restricted,and the poor electrochemical performance of the anode materials at low temperature serves as a critical ***,it is urgent to obtain a comprehensive understanding towards the key effects of low temperatures on the performance of the anodes and overview the related improving *** this work,the effects that temperature would impose on electrode performance are firstly ***,the progress in low-temperature anodes of alkali metal ion batteries is reviewed,by the classification of the reaction types of the anode materials,including intercalation-type anodes,conversion-type anodes,alloy anodes and alkali metal anodes,and corresponding strategies to improve the performance of the anodes are summarized as *** last,some promising research directions in this field are *** work is intended to shed some light on future exploitation of high-performance low-temperature anode materials.

关键词： Anode Alkali metal ion batteries Electrode materials Graphite Low temperature