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Identification of QTLs for Starch Content in Sweetpotato(Ipomoea batatas(L.) Lam.)

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Journal of Integrative Agriculture 2014年第2期13卷 310-315页

作者： yu xiao-xia ZHAO Ning LI Hui JIE Qin ZHAI Hong HE Shao-zhen LI Qiang LIU Qing-chang Laboratory of Crop Heterosis and Utilization Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/China Agricultural University Xuzhou Sweetpotato Research Center

Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

关键词： co-localized marker lpomoea batatas (L.) Lam. QTLs starch content

Effects of ferulic acid on antioxidant activity in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and their combination

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Chinese Journal of Natural Medicines 2015年第6期13卷 401-408页

作者： WANG Lin-Yan TANG yu-Ping LIU Xin ZHU Min TAO Wei-Wei LI Wei-xia DUAN Jin-Ao Jingjiang Hospital of Traditional Chinese Medicine Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine Nanjing University of Chinese Medicine

The present study aimed at exploring different roles of the same compound in different environment, using preparative HPLC, and the significance to investigating bio-active constituents in traditional Chinese medicine （TCM） on the basis of holism. In this study, the depletion of target component ferulic acid （FA） by using preparative HPLC followed by antioxidant activity testing was applied to investigate the roles of FA in Angelicae Sinansis Radix （DG）, Chuanxiong Rhizoma （CX） and their combination （GX）. The antioxidant activity was performed by 1, 1-diphenyl-2-picrylhydrazyl （DPPH） radical-scavenging activity testing. FA was successfully and exclusively depleted from DG, CX, and GX, respectively. By comparing the effects of the samples, it was found that FA was one of the main antioxidant constituents in DG, CX and GX, and the roles of FA were DG 〉 CX 〉 GX. Furthermore, the effects of FA varied at different doses in these herbs. This study provided a reliable and effective approach to clarifying the contribution of same compound in different TCMs to their bio-activities. The role of a constituent in different TCMs might be different, and a component with the same content might have different effects in different chemical environments. Furthermore, this study also suggested the potential utilization of preparative HPLC in the characterization of the roles of multi-ingredients in TCM.

关键词： HPLC Ferulic acid Angelica sinensis Ligusticum chuanxiong Herb pair Antioxidant activity

Facile and Low-Cost Fabrication of a Thread/Paper-Based Wearable System for Simultaneous Detection of Lactate and pH in Human Sweat

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Advanced Fiber Materials 2020年第5期2卷 265-278页

作者： Gang xiao Jing He Yan Qiao Feng Wang Qingyou xia Xin Wang Ling yu Zhisong Lu Chang-Ming Li Institute for Clean Energy and Advanced Materials School of Materials and EnergySouthwest University1 Tiansheng RoadChongqing 400715People’s Republic of China State Key Laboratory of Silkworm Genome Biology Southwest University1 Tiansheng RoadChongqing 400715People’s Republic of China Chongqing Engineering and Technology Research Center for Novel Silk Materials Southwest University1 Tiansheng RoadChongqing 400715People’s Republic of China College of Food Science Southwest UniversityNo.2 Tiansheng RoadBeibei DistrictChongqing 400715People’s Republic of China Institute of Advanced Cross-Field Science Qingdao UniversityQingdao 266071People’s Republic of China

Wearable devices have received tremendous interests in human sweat analysis in the past few ***,the widely used polymeric substrates and the layer-by-layer stacking structures greatly influence the cost-efficiency,conformability and breathability of the devices,further hindering their practical ***,we report a facile and low-cost strategy for the fabrication of a skin-friendly thread/paper-based wearable system consisting of a sweat reservoir and a multi-sensing component for simultaneous in situ analysis of sweat pH and *** the system,hydrophilic silk thread serves as the micro-channel to guide the liquid *** papers were functionalized to prepare colorimetric sensors for lactate and *** smartphone-based quantitative analysis shows that the sensors are sensitive and *** pH may interfere the lactate detection,the pH detected simultaneously could be employed to correct the measured data for the achievement of a precise lactate *** being integrated with a hydrophobic arm guard,the system was successfully used for the on-body measurement of pH and lactate in the sweats secreted from the *** low-cost,easy-to-fabricate,light-weight and flexible thread/paper-based microfluidic sensing device may hold great potentials as a wearable system in human sweat analysis and point-of-care diagnostics.

关键词： Wearable sensors Thread/paper-based microfluidics Sweat analysis Point-of-care diagnostics Multi-sensing system

PPOS方案促排卵过程中添加重组人生长激素注射液对卵巢低反应患者IVF结局的影响

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实用临床医学（江西） 2024年第4期25卷 63-67页

作者：张玉菡伍琼芳夏雷震苏琼江西省妇幼保健院辅助生殖中心南昌330000

目的探讨在卵泡期高孕激素状态下促排卵(PPOS)方案中联合使用重组人生长激素注射液对卵巢低反应患者体外受精-胚胎移植结局的影响。方法选取2020-2022年在江西省妇幼保健院辅助生殖中心进行助孕治疗的患者2137例,按照是否在PPOS过程中... 详细信息

目的探讨在卵泡期高孕激素状态下促排卵(PPOS)方案中联合使用重组人生长激素注射液对卵巢低反应患者体外受精-胚胎移植结局的影响。方法选取2020-2022年在江西省妇幼保健院辅助生殖中心进行助孕治疗的患者2137例,按照是否在PPOS过程中使用重组人生长激素注射液分为试验组(n=730)和对照组(n=1407)。对2组的临床特征及治疗结局进行回顾性PSM分析。结果试验组获卵数(3.87±2.93 vs3.21±2.44,P<0.001)、可移植胚胎数(1.47±1.18vs1.31±1.12,P=0.008)、HCG阳性率(64.0%vs52.7%,P=0.004)均显著高于对照组。结论PPOS方案中联合使用重组人生长激素注射液可增加卵巢低反应患者获卵数,显著改善卵母细胞和胚胎的数量及质量,改善临床结局。

关键词：体外受精高孕激素状态下促排卵重组人生长激素注射液卵巢低反应

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发酵饲料在动物生产中应用的研究进展

饲料研究 2024年第4期47卷 154-157页

作者：郁冯艳付佳伟从光雷刘春雪夏双双杜莉李俊波安佑生物科技集团股份有限公司江苏苏州215400

发酵饲料具有绿色、安全、适口性好、促生长、增强免疫力、降本增效等优点,可作为抗生素替代品在动物生产中应用。文章主要概述了发酵饲料的常用菌种、分类、品质评价、生理功能以及发酵饲料在动物生产中的作用效果,分析当前发酵饲料存... 详细信息

发酵饲料具有绿色、安全、适口性好、促生长、增强免疫力、降本增效等优点,可作为抗生素替代品在动物生产中应用。文章主要概述了发酵饲料的常用菌种、分类、品质评价、生理功能以及发酵饲料在动物生产中的作用效果,分析当前发酵饲料存在的问题及解决办法,展望发酵饲料的发展趋势,为发酵饲料在动物生产中更广泛的应用提供参考。

关键词：发酵饲料肠道菌群动物消化代谢生产性能

High-quality chromosome-level genome assembly of the melon-headed whale(Peponocephala electra)

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Zoological Research 2022年第6期43卷 931-934页

作者： Zhen-Peng yu Xing Liu Biao Zhang Lei Shan Inge Seim Guang Yang Shi-xia Xu Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life SciencesNanjing Normal UniversityNanjingJiangsu 210023China

DEAR EDITOR,The melon-headed whale(Peponocephala electra),a small toothed whale in the Delphinidae family,inhabits tropical and subtropical *** is an attractive model species for studying secondary aquatic adaptation and ***,we successfully assembled a high-quality chromosome-level genome of *** using Pac Bio and Hi-C sequencing technologies.

关键词： evolution subtropical tropical

Renal subcapsular haematoma： an unusual complication of renal artery stenting

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Chinese Medical Journal 2011年第9期124卷 1438-1440页

作者： xia dan CHEN Shan-wen ZHANG Hong-kun WANG Shuo Department of Urology First Affiliated Hospital Medical College of Zhejiang University HangzhouZhejiang 310031 China Department of Vascular Surgery First Affiliated Hospital Medical College of Zhejiang University HangzhouZhejiang 310031 China

After successful renal artery angioplasty and stent placement, a patient in a fully anticoagulated state developed hypotension and flank pain. Computed tomography （CT） of the abdomen revealed a large renal subcapsular haematoma which was successfully managed conservatively without embolotherapy and surgical intervention. To prevent hemorrhage after renal artery stenting, it is necessary to underscore the importance of reducing the contrast volume and pressure of angiography, controlling systemic blood pressure, and monitoring guide wire position at all times.

关键词： stent haematoma renal artery stenosis

Expression profiles of Cry1Ab protein and its insecticidal efficacy against the invasive fall armyworm for Chinese domestic GM maize DBN9936

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Journal of Integrative Agriculture 2021年第3期20卷 792-803页

作者： LIANG Jin-gang ZHANG dan-dan LI Dong-yang ZHAO Sheng-yuan WANG Chen-yao xiaO yu-tao XU Dong YANG Yi-zhong LI Guo-ping WANG Li-li GAO yu YANG Xue-qing yuAN Hai-bin LIU Jian ZHANG Xiu-jie WU Kong-ming Development Center of Science and Technology Ministry of Agriculture and Rural AffairsBeijing 100176P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000P.R.China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China Institute of Plant Protection and Soil Science Hubei Academy of Agricultural SciencesWuhan 430064P.R.China College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009P.R.China Institute of Plant Protection Henan Academy of Agricultural SciencesZhengzhou 450002P.R.China Yantai Academy of Agricultural Sciences Yantai 265500P.R.China College of Plant Protection Shenyang Agricultural UniversityShenyang 110866P.R.China College of Plant Protection Jilin Agricultural UniversityChangchun 130118P.R.China College of Agriculture Northeast Agricultural UniversityHarbin 150030P.R.China

The fall armyworm(FAW)Spodoptera frugiperda,which originated in the Americas,is advancing across China and threatening the nation’s maize ***,one widely used tool for its control is genetically modified(GM)Bacillus thuringiensis(Bt)*** content of Bt protein in appropriate plant parts is crucial for enhancing resistance against insect *** this study,we conducted a systematic investigation of Cry1 Ab levels in Chinese domestic GM maize DBN9936,which has recently obtained a biosafety certificate,and evaluated its efficacy against *** of expression levels of Cry1 Ab,via ELISA,indicated a spatio-temporal dynamic,with significant variation of mean Cry1 Ab,ranging from 0.76 to 8.48μg g-1 FW with the Cry1 Ab protein level ranked as:V6-V8 leaf>R1 leaf>R4 leaf>R1 silk>VT tassel>R4 *** the nine locations,the Cry1 Ab levels in DBN9936 of the Xinxiang,Langfang,and Harbin fields were significantly lower than those from Wuhan and Shenyang,and were slightly,but not significantly lower than those from the other four ***,the artificial diet-Cry1 Ab mixture and plant tissue feeding bioassays revealed that DBN9936 has high efficacy against *** insecticidal efficacy of different tissues against FAW larvae reached 34-100%with a descending order of lethality as follows:VT leaf>R4 leaf>R1 husk>R1 silk>VT tassel>R4 *** together,our results showed that Bt-Cry1 Ab maize DBN9936 has potential as a promising strategy to manage FAW.

关键词： fall armyworm genetically modified maize DBN9936 Cry1Ab expression control efficacy

并发症与死亡病例讨论会在口腔颌面外科住院医师规范化培训中的应用

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中国高等医学教育 2024年第4期 73-74,84页

作者：余丹沈文怡黄建瑶朱慧勇倪幼康浙江大学医学院附属第一医院浙江杭州310000 浙江大学医学院附属第四医院浙江义乌310000

目的:探讨并发症与死亡病例讨论会(morbidity and mortality conferences,双M讨论会)在口腔颌面外科住院医师规范化培训的教学效果。方法:2021年8月至2022年8月,每周定期举行双M讨论会。通过出科考核评价参加双M讨论会学员(试验组)和未... 详细信息

目的:探讨并发症与死亡病例讨论会(morbidity and mortality conferences,双M讨论会)在口腔颌面外科住院医师规范化培训的教学效果。方法:2021年8月至2022年8月,每周定期举行双M讨论会。通过出科考核评价参加双M讨论会学员(试验组)和未参加讨论学员(对照组)的临床综合能力。并发放调查问卷进行讨论会满意度调查。结果:出科考核结果显示试验组成绩显著性高于对照组(P=0.031)。97.67%的试验组学员认为有必要经常开展。结论:双M讨论会可有效提高教学质量和满意度。

关键词：并发症与死亡病例讨论会口腔颌面外科规范化培训临床教学