检索结果-南通市图书馆

Ocellatuspyrones A‒G,new antibacterial polypropionates from the Chinese mollusk Placobranchus ocellatus

在线全文

学校读者我要写书评

暂无评论

Marine Life Science & Technology 2023年第3期5卷 373-386页

作者： Song-Wei Li dan-dan yu Ming-Zhi Su Li-Gong Yao Hong Wang Xueting Liu yue-Wei Guo Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals College of Pharmaceutical ScienceZhejiang University of TechnologyHangzhou 310014China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantai 264117China

Marine invertebrates serve as rich sources of secondary metabolites with intriguing chemical diversities and a wide spectrum of biological activities.Particularly,marine shell-less sacoglossan mollusks have attracted much attentions due to the fact that mollusks apply complex metabolites as chemical defense agents against to their predators.With the purpose of discovering bioactive secondary metabolites to develop marine-derived medicines from the South China Sea,we have conducted a chemical study on the photosynthetic mollusk Placobranchus ocellatus.As a result,seven newγ-pyrone polypropionates,namely(±)-ocellatuspyrone A(1),(±)-ocellatuspyrone B(2),and ocellatuspyrones C−G(5,9−12),along with fve known polypropionates,have been isolated and characterized from the South China Sea photosynthetic mollusk Placobranchus ocellatus.Extensive spectroscopic analysis,single crystal X-ray difraction analysis,modifed Mosher’s method,ECD comparison,CD exciton chirality method,TDDFT-ECD calculation,and chemical conversion were used to determine the structures and absolute confgurations of the new compounds and the stereochemistry of undefned known compounds 4,6 and 7.All these isolated polypropionates were evaluated in bioassays for their biological activities,including antibacterial,neuroprotective efect,anti-infammatory,PTP1B inhibitory,and antiviral activities.Compounds 7,8 and 11 were found for the frst time to show antibacterial activity against fsh pathogenic bacteria Streptococcus parauberis(the main pathogen causing fsh streptococcal infections and acute death)with MIC values of 35.8,34.2,and 37.4μg/mL,respectively,which might be potential novel antibacterial agents for the treatment of fsh infectious diseases.

关键词： Placobranchus ocellatus γ-pyrone polypropionate Photosynthetic mollusk Antibacterial activity

Comprehensive Treatment Uncertainty Analysis and PTV Margin Estimation for Fiducial Tracking in Robotic Liver Stereotactic Body Radiation Therapy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

当代医学科学(英文) 2023年第3期43卷 572-578页

作者： Zhi-wen LIANG Meng-lan ZHAI Biao TU Xin NIE Xiao-hui ZHU Jun-ping CHENG Guo-quan LI dan-dan yu Tao ZHANG Sheng ZHANG Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Objective This study aims to quantify the uncertainties of CyberKnife Synchrony fiducial tracking for liver stereotactic body radiation therapy(SBRT)cases,and evaluate the required planning target volume(PTV)margins.Methods A total of 11 liver tumor patients with a total of 57 fractions,who underwent SBRT with synchronous fiducial tracking,were enrolled for the present study.The correlation/prediction model error,geometric error,and beam targeting error were quantified to determine the patient-level and fraction-level individual composite treatment uncertainties.The composite uncertainties and multiple margin recipes were compared for scenarios with and without rotation correction during treatment.Results The correlation model error-related uncertainty was 4.3±1.8,1.4±0.5 and 1.8±0.7 mm in the superior-inferior(SI),left-right,and anterior-posterior directions,respectively.These were the primary contributors among all uncertainty sources.The geometric error significantly increased for treatments without rotation correction.The fraction-level composite uncertainties had a long tail distribution.Furthermore,the generally used 5-mm isotropic margin covered all uncertainties in the left-right and anterior-posterior directions,and only 75%of uncertainties in the SI direction.In order to cover 90%of uncertainties in the SI direction,an 8-mm margin would be needed.For scenarios without rotation correction,additional safety margins should be added,especially in the superior-inferior and anterior-posterior directions.Conclusion The present study revealed that the correlation model error contributes to most of the uncertainties in the results.Most patients/fractions can be covered by a 5-mm margin.Patients with large treatment uncertainties might need a patient-specific margin.

关键词： CyberKnife fiducial tracking liver cancer stereotactic body radiation therapy margin expansion

Single-nucleus transcriptomic profiling of multiple organs in a rhesus macaque model of SARS-CoV-2 infection

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Zoological Research 2022年第6期43卷 1041-1062页

作者： Qiang Ma Wenji Ma Tian-Zhang Song Zhaobo Wu Zeyuan Liu Zhenxiang Hu Jian-Bao Han Ling Xu Bo Zeng Bosong Wang Yinuo Sun dan-dan yu Qian Wu Yong-Gang Yao Yong-Tang Zheng Xiaoqun Wang State Key Laboratory of Brain and Cognitive Science Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Kunming National High-Level Biosafety Research Center for Non-Human Primates Center for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China LivzonBio Inc.ZhuhaiGuangdong 519045China State Key Laboratory of Cognitive Neuroscience and Learning Beijing Normal UniversityBeijing 100875China IDG/McGovern Institute for Brain Research Beijing Normal UniversityBeijing 100875China Advanced Innovation Center for Human Brain Protection Beijing Institute for Brain DisordersCapital Medical UniversityBeijing 100069China

Infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) causes diverse clinical manifestations and tissue injuries in multiple organs.However, cellular and molecular understanding of SARS-CoV-2 infection-associated pathology and immune defense features in different organs remains incomplete. Here, we profiled approximately 77 000single-nucleus transcriptomes of the lung, liver,kidney, and cerebral cortex in rhesus macaques(Macaca mulatta) infected with SARS-CoV-2 and healthy controls. Integrated analysis of the multiorgan dataset suggested that the liver harbored the strongest global transcriptional alterations. We observed prominent impairment in lung epithelial cells, especially in AT2 and ciliated cells, and evident signs of fibrosis in fibroblasts. These lung injury characteristics are similar to those reported in patients with coronavirus disease 2019(COVID-19).Furthermore, we found suppressed MHC class I/II molecular activity in the lung, inflammatory response in the liver, and activation of the kynurenine pathway,which induced the development of an immunosuppressive microenvironment. Analysis of the kidney dataset highlighted tropism of tubule cells to SARS-CoV-2, and we found membranous nephropathy(an autoimmune disease) caused by podocyte dysregulation. In addition, we identified the pathological states of astrocytes and oligodendrocytes in the cerebral cortex, providing molecular insights into COVID-19-related neurological implications. Overall, our multi-organ single-nucleus transcriptomic survey of SARS-CoV-2-infected rhesus macaques broadens our understanding of disease features and antiviral immune defects caused by SARS-CoV-2 infection,which may facilitate the development of therapeutic interventions for COVID-19.

关键词： SARS-CoV-2 Rhesus macaque Animal model Single-nucleus RNA sequencing Antiviral immune defects Multiple organs

Positively selected genes of the Chinese tree shrew （Tupaia belangeri chinensis） locomotion system

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2014年第3期35卷 240-248页

作者： yu FAN dan-dan yu Yong-Gang YAO Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & yunnan Province Kunming Institute of Zoology Kunming Yunnan 650223 China Kunming College of Life Science University of Chinese Academy of Sciences Kunming Yunnan 650223 China Kunming Primate Research Center Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China

While the recent release of the Chinese tree shrew(Tupaia belangeri chinensis) genome has made the tree shrew an increasingly viable experimental animal model for biomedical research, further study of the genome may facilitate new insights into the applicability of this model. For example, though the tree shrew has a rapid rate of speed and strong jumping ability, there are limited studies on its locomotion ability. In this study we used the available Chinese tree shrew genome information and compared the evolutionary pattern of 407 locomotion system related orthologs among five mammals(human, rhesus monkey, mouse, rat and dog) and the Chinese tree shrew. Our analyses identified 29 genes with significantly high ω(Ka/Ks ratio) values and 48 amino acid sites in 14 genes showed significant evidence of positive selection in the Chinese tree shrew. Some of these positively selected genes, e.g. HOXA6(homeobox A6) and AVP(arginine vasopressin), play important roles in muscle contraction or skeletal morphogenesis. These results provide important clues in understanding the genetic bases of locomotor adaptation in the Chinese tree shrew.

关键词：基因组信息运动系统树鼩中国正选择实验动物模型精氨酸加压素运动能力

A virtual 3D interactive painting method for Chinese calligraphy and painting based on real-time force feedback technology

在线全文

学校读者我要写书评

暂无评论

Frontiers of Information Technology & Electronic Engineering 2017年第11期18卷 1843-1853页

作者： Chao GUO Zeng-xuan HOU You-zhi SHI Jun XU dan-dan yu School of Mechanical Engineering Dalian University of Technology Dalian 116024 China

本文提出了一种新的基于实时力反馈技术的中国书画三维交互绘制方法。本文通过分析毛笔受力与变形的关系,基于一种弹簧振子模型构建了三维毛笔模型。根据毛笔受力产生的变形计算得到毛笔在绘制平面上形成的2D笔触,并将其实时映射到3D物... 详细信息

本文提出了一种新的基于实时力反馈技术的中国书画三维交互绘制方法。本文通过分析毛笔受力与变形的关系,基于一种弹簧振子模型构建了三维毛笔模型。根据毛笔受力产生的变形计算得到毛笔在绘制平面上形成的2D笔触,并将其实时映射到3D物体表面形成3D笔触,沿着绘制方向叠加3D笔触形成3D笔道。本方法已成功应用于基于实时力反馈技术的虚拟三维交互绘制系统,用户可以通过Phantom Desktop力反馈设备实时绘制三维笔道,从而实现对三维交互绘制过程的有效仿真。

关键词：三维毛笔模型三维笔道三维交互绘制实时力反馈技术

Shenqi Fuzheng Injection Ameliorates Radiation-induced Brain Injury

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2019年第6期39卷 965-971页

作者： Ling-juan CHEN Rui-guang ZHANG dan-dan yu Gang WU Xiao-rong DONG Cancer Center Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Shenqi Fuzheng injection(SFI)has been confirmed to be able to alleviate brain injury in mice.This study examined the brain-protective effect of SFI on patients after cranial radiation.Lung cancer patients with brain metastasis were randomly assigned to two groups.The SFI group received cranial radiation in combination with SFI.The control group received cranial radiation alone.The changes in cognitive function were evaluated pre- and post-radiation against the Mini-Mental State Exam(MMSE),Montreal Cognitive Assessement(MoCA),Zung Self-Rating Depression Scale(SDS)and Zung Self-Rating Anxiety Scale(SAS).The changes in inflammatory factors,such as TGF-β1,TNF-α and IL-10,were also detected before,during and after radiation(15Gy/5F).The results showed that 6 months after cranial radiation,the total scores on the MMSE and MoCA scales of the patients decreased,especially memory ability.The control group experienced a more evident decline,the memory ability being the greatest.TGF-β1 and TNF-α increased shortly after radiation and decreased one month later,and the change was more conspicuous in SFI group than in control group.IL-10 increased after radiation and stayed at a high level one month later in both groups,the level being higher in the SFI group than in the control group.Our study indicated that cognitive functions,especially memory ability,were impaired after cranial radiation.SFI could alleviate radiation-induced brain injury by regulating inflammatory factors.

关键词： Shenqi Fuzheng injection irradiation-induced brain injury cognitive disorders inflammation

Evodiamine inhibits high-fat diet-induced colitis-associated cancer in mice through regulating the gut microbiota

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine 2021年第1期19卷 56-65页

作者： Li-qing Zhu Li Zhang Jia Zhang Guo-lin Chang Gang Liu dan-dan yu Xiao-min yu Mi-sheng Zhao Bin Ye Department of Pathogenic Biology Chongqing Medical UniversityChongqing 400016China Research Center for Molecular Medicine and TumorChongqing Medical UniversityChongqing 400016China Department of Clinical LaboratoryThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325023Zhejiang ProvinceChina Department of PathophysiologyChongqing Medical UniversityChongqing 400016China Department of Clinical LaboratoryWenzhou People’s HospitalWenzhou 325023Zhejiang ProvinceChina Department of EmergencyUniversity-Town Hospital of Chongqing Medical UniversityChongqing 401331China

Objective:High-fat diet is one of the main risk factors that disrupt the balance of gut microbiota,which eventually will induce colorectal cancer(CRC).Evodiamine(EVO)is a wildly used multifunctional traditional Chinese medicine extract.In this study,we investigated the role of gut microbiota in high-fat dietpropelled CRC and the potential of EVO for CRC chemoprevention.Methods:Gut microbiota,serum D-lactic acid and endotoxin from 38 patients with colon cancer and 18 healthy subjects were detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA).In addition,body mass index,phospho-signal transducer and activator of transcription 3(p-STAT3)expression in cancer tissues and paracancerous tissues were detected by immunohistochemistry.A mouse intestinal inflammatory tumor model was established by azomethane/sodium dextran sulfate,followed by treatment with EVO and 5-aminosalicylic acid(ASA).Gut microbiota and inflammatory factors were detected by quantitative polymerase chain reaction,while serum D-lactic acid and endotoxin were detected by ELISA.Furthermore,cell proliferation,cell apoptosis,and interleukin(IL)-6/STAT3/P65 pathway were evaluated by 5-ethynyl-20-deoxyuridine,terminal-deoxynucleotidyl transferase-mediated nick-end labeling,and Western blot assays.Results:In patients with colon cancer,the numbers of Enterococcus faecalis and Escherichia coli were increased,while those of Bifidobacterium,Campylobacter and Lactobacillus were decreased.Serum endotoxin and D-lactic acid levels and p-STAT3 levels were significantly increased.In the mouse model,both EVO and ASA inhibited tumor formation,decreased the proliferation of tumor cells,and induced apoptosis of tumor cells.Compared with the control group,the numbers of E.faecalis and E.coli were decreased,while Bifidobacterium,Campylobacter and Lactobacillus numbers were increased.In the EVO group,serum endotoxin and D-lactic acid levels and inflammatory factors were significantly decreased.Further,the IL6/STAT3/P65 signa

关键词： Evodiamine Gut microbiota Colitis-associated cancer Signal transducer and activator of transcription 3

Comprehensive annotation of the Chinese tree shrew genome by large-scale RNA sequencing and long-read isoform sequencing

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2021年第6期42卷 692-709页

作者： Mao-Sen Ye Jin-Yan Zhang dan-dan yu Min Xu Ling Xu Long-Bao Lv Qi-yun Zhu yu Fan Yong-Gang Yao Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences&yunnan Province KIZ/CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650204China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals Primate Facility) Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650107China 4 State Key Laboratory of Veterinary Etiological BiologyLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouGansu 730046China

The Chinese tree shrew(Tupaia belangeri chinensis)is emerging as an important experimental animal in multiple fields of biomedical research.Comprehensive reference genome annotation for both mRNA and long non-coding RNA(lncRNA)is crucial for developing animal models using this species.In the current study,we collected a total of 234 high-quality RNA sequencing(RNA-seq)datasets and two long-read isoform sequencing(ISO-seq)datasets and improved the annotation of our previously assembled high-quality chromosomelevel tree shrew genome.We obtained a total of 3514 newly annotated coding genes and 50576 lncRNA genes.We also characterized the tissuespecific expression patterns and alternative splicing patterns of mRNAs and lncRNAs and mapped the orthologous relationships among 11 mammalian species using the current annotated genome.We identified 144 tree shrew-specific gene families,including interleukin 6(IL6)and STT3 oligosaccharyltransferase complex catalytic subunit B(STT3B),which underwent significant changes in size.Comparison of the overall expression patterns in tissues and pathways across four species(human,rhesus monkey,tree shrew,and mouse)indicated that tree shrews are more similar to primates than to mice at the tissue-transcriptome level.Notably,the newly annotated purine rich element binding protein A(PURA)gene and the STT3B gene family showed dysregulation upon viral infection.The updated version of the tree shrew genome annotation(KIZ version 3:TS_3.0)is available at http://*** and provides an essential reference for basic and biomedical studies using tree shrew animal models.

关键词： Tree shrew Genome annotation Transcriptome Gene family Virus infection

Chromosomal level assembly and population sequencing of the Chinese tree shrew genome

在线全文

学校读者我要写书评

暂无评论

Zoological Research 2019年第6期40卷 506-521页

作者： yu Fan Mao-Sen Ye Jin-Yan Zhang Ling Xu dan-dan yu Tian-Le Gu yu-Lin Yao Jia-Qi Chen Long-Bao Lv Ping Zheng Dong-Dong Wu Guo-Jie Zhang Yong-Gang Yao Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming Yunnan 650223China Kunming College of Life Science University of Chinese Academy of SciencesKunming Yunnan 650204China Kunming Primate Research Center of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China KIZ–CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China yunnan Key Laboratory of Animal Reproduction Kunming Institute of ZoologyChinese Academy of SciencesKunming Yunnan 650223China

Chinese tree shrews (Tupaia belangeri chinensis) have become an increasingly important experimental animal in biomedical research due to their close relationship to primates. An accurately sequenced and assembled genome is essential for understanding the genetic features and biology of this animal. In this study, we used long-read single-molecule sequencing and high-throughput chromosome conformation capture (Hi-C) technology to obtain a high-qualitychromosome-scale scaffolding of the Chinese tree shrew genome. The new reference genome (KIZ version 2: TS_2.0) resolved problems in presently available tree shrew genomes and enabled accurate identification of large and complex repeat regions, gene structures, and species-specific genomic structural variants. In addition, by sequencing the genomes of six Chinese tree shrew individuals, we produced a comprehensive map of 12.8 M single nucleotide polymorphisms and confirmed that the major histocompatibility complex (MHC) loci and immunoglobulin gene family exhibited high nucleotide diversity in the tree shrew genome. We updated the tree shrew genome database (TreeshrewDB v2.0: http://***) to include the genome annotation information and genetic variations. The new high-quality reference genome of the Chinese tree shrew and the updated TreeshrewDB will facilitate the use of this animal in many different fields of research.

关键词： Tupaia belangeri Chromosomal level assembly genome Population sequencing Database