检索结果-南通市图书馆

Smoke movement in a tunnel of a running metro train on fire

在线全文

学校读者我要写书评

暂无评论

Journal of Central South University 2015年第1期22卷 208-213页

作者：周丹田红旗郑晋丽颜鑫 Key Laboratory of Traffic Safety on Track of Ministry of Education(School of Traffic and Transportation Engineering Central South University) Shanghai Tunnel Engineering & Rail Transit Design and Research Institute

Research on the distribution of smoke in tunnels is significant for the fire emergency rescue after an operating metro train catches fire. A dynamic grid technique was adopted to research the law of smoke flow diffusion inside the tunnel when the bottom of a metro train was on fire and to compare the effect of longitudinal ventilation modes on the smoke motion when the burning train stopped. Research results show that the slipstream curves around the train obtained by numerical simulation are consistent with experimental data. When the train decelerates, the smoke flow first extends to the tail of the train. With the decrease of the train's speed, the smoke flow diffuses to the head of the train. After the train stops, the slipstream around the train formed in the process of train operation plays a leading role in the smoke diffusion in the tunnel. The smoke flow quickly diffuses to the domain in front of the train. After forward mechanical ventilation is provided, the smoke flow inside the tunnel continues to diffuse downstream. When reverse mechanical ventilation operates, the smoke in front of the train flows back rapidly and diffuses to the rear of the train.

关键词： subway tunnel train catching fire moving fire source smoke movement ventilation control

Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第5期10卷 2137-2150页

作者： Yunyi liu Juan li Hailong Ou dan Qi Bei Hu Yuxi Xu Jian Hu Yi Xiong Luling Xia Jason HHuang Xiaoxiao Hu Erxi Wu State Key Laboratory of Chemo/Biosensing and Chemometrics College of BiologyMolecular Science and Biomedicine Laboratory and Aptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082China Shenzhen Research Institute Hunan UniversityShenzhenGuangdong 518000China Department of Neurosurgery and Neuroscience Institute Baylor Scott&White HealthTempleTX 76508USA Department of Respiratory Medicine The Third Xiangya HospitalCentral South UniversityChangshaHunan 410013China Texas A&M University School of Medicine College StationTX 77843USA Texas A&M University School of Pharmacy College StationTX 77843USA liVESTRONG Cancer Institutes and Department of Oncology Dell Medical SchoolThe University of Texas at AustinAustinTX 78712USA Greater Bay Area Institute for Innovation Hunan UniversityGuangzhouGuangdong 511300China

Aptamers,short single DNA or RNA oligonucleotides,have shown immense application potential as molecular probes for the early diagnosis and therapy of ***,conventional cell-SELEX technologies for aptamer discovery are time-consuming and *** we discovered a new aptamer BC-3 by using an improved rapid X-Aptamer selection process for human bladder carcinoma,for which there is no specific molecular probe *** show that BC-3 exhibited excellent affinity in bladder cancer cells but not normal *** demonstrate that BC-3 displayed high selectivity for tumor cells over their normal counterparts in vitro,in mice,and in patient tumor tissue *** endocytosis pathway analysis revealed that BC-3 internalized into bladder cancer cells via clathrin-mediated ***,we identified ribosomal protein S7(RPS7)as the binding target of BC-3 via an integrated methodology(mass spectrometry,colocalization assay,and immunoblotting).Together,we report that a novel aptamer BC-3 is discovered for bladder cancer and its properties in the disease are *** findings will facilitate the discovery of novel diagnostic and therapeutic strategies for bladder cancer.

关键词： Bladder cancer Cell-SELEX Clathrin-mediated endocytosis Intracellular colocalization Ribosomal protein S7 X-aptamer selections

Dynamic Organization and Methodology for Agile Virtual Enterprises

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Computer Science & Technology 2000年第4期15卷 368-375页

作者：徐晓飞叶丹李全龙战德臣 Department of Computer Science & Engineering Harbin Institute of Technology Harbin P.R. China

With the trend of the worldwide market competition, the globalagile manufacturing will become an advanced manufacturing technology in 21st century, and the Agile Virtual Enterprise (AVE) will also become a new organizationform of manufacturing enterprises. As AVE is a complicated enierprise, how tobuild an optimal AVE organization is a difficult problem. In this paper, based onthe analysis of the AVE organization, the methodology for AVE including enterprisearchitecture, reference model, enterprise modeling methods and toolkit, guideline forsystem implementation is proposed. This paper also presents a Virtual Organization Integrated Support Environment (VOISE), which provides the computer-aidedsupport for rapidly building an optimal AVE.

关键词： AM (Agile Manufacturing) AVE (Agile Virtual Enterprise) dynamic organization methodology

Outpatient Surgery in Zhongshan Ophthalmic Center： Promise and Problems

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2017年第13期130卷 1623-1624页

作者： Zhi-Gang Fan dan-dan Zhang Zhong-Wen li Yao Yang Xiao-Feng lin State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou Guangdong 510060 China

To the Editor： The Chinese government is actively advocating medical reform, aiming at reducing medical costs, and improving service quality. As to ophthalmology, transition from traditional hospitalized surgery to a predominant outpatient mode could be a solution. Zhongshan Ophthalmic Center （ZOC） is one of the largest and leading ophthalmic centers in China,

关键词： Outpatient Surgery Zhongshan Ophthalmic Center Promise and Problems

Pterostilbene supresses inflammation-induced melanoma metastasis by impeding neutrophil elastase-mediated thrombospondin-1 degradation

在线全文

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2023年第1期15卷 94-101页

作者： dan Su Shan Xu Kailin Ji Hailing Xu Yan li Zhisheng Zhang Yuqing Shen Gaoyang Chen Department of Pharmacy Changzhou No.2 People’s HospitalAffiliated Hospital of Nanjing Medical UniversityChangzhou 213004China School of Pharmacy Changzhou UniversityChangzhou 213164China Department of Oncology The Second People’s Hospital of TaizhouTaizhou 225300China

Objective: Chronic inflammation plays a fatal role in tumor metastasis. Pterostilbene(PTE) is a natural dimethylated analogue of resveratrol with anticancer and anti-inflammatory activities. This study aimed to investigate the inhibitory effect of PTE on inflammation-associated metastasis and explore the underlying ***: lipopolysaccharide(LPS)-induced lung inflammation and melanoma metastasis models were established in mice. After PTE treatment for four weeks, the organ index, histological changes, proinflammatory cytokines, and the expression and activity of neutrophil elastase(NE), a biomarker of neutrophil influx in the lungs, were analysed. Additionally, direct effects of PTE on NE-induced B16 cell migration were explored in wound healing and Transwell assays, and the expression of thrombospondin-1(TSP-1) and epithelial-mesenchymal transition(EMT) markers were also ***: PTE obviously attenuated the LPS-induced metastasis of circulatory B16 cells to lungs by reducing the number of metastatic nodules on the lung surfaces and the lung weight/body weight ratio. PTE treatment also significantly reduced LPS-activated increase levels of tumor necrosis factor(TNF)-a and interleukin(IL)-6 in the lungs of tumor-bearing mice. In addition, increased expression and enzyme activity of NE and decreased expression of TSP-1 were observed, and these were blocked by PTE. In vitro, PTE at concentrations without cytotoxicity also markedly suppressed NE-triggered B16 cell migration, prevented NE-induced TSP-1 proteolysis and reversed the expression of vimentin, N-cadherin and ***: PTE could block inflammation-enhanced tumor metastasis, and the underlying mechanism might be associated with the inhibition of NE-mediated TSP-1 degradation.

关键词： inflammation melanoma metastasis neutrophil elastase pterostilbene thrombospondin-1

基于分形理论的蚀变异常遥感解译——以广东阳山县为例

在线全文

学校读者我要写书评

暂无评论

华南地质 2024年第2期40卷 380-392页

作者：祝一丹吴志春马粉玲李华亮秦耀祖东华理工大学地球科学学院江西南昌330013

围岩蚀变异常是热液型矿床的重要找矿标志,运用遥感技术解译提取围岩蚀变信息是辅助地质找矿的重要手段。本文采用主成分分析法提取广东阳山地区Landsat-5的TM遥感影像中铁染和泥化蚀变信息,对影像中所提取的异常主成分进行“像元-面积... 详细信息

围岩蚀变异常是热液型矿床的重要找矿标志,运用遥感技术解译提取围岩蚀变信息是辅助地质找矿的重要手段。本文采用主成分分析法提取广东阳山地区Landsat-5的TM遥感影像中铁染和泥化蚀变信息,对影像中所提取的异常主成分进行“像元-面积”划分并统计像元个数,再将异常阈值进行分形非线性划分,得到更为精准的分级方法。门限法是基于突变点选择阈值进行等级划分,在提取蚀变异常的结果中往往会包含大量的背景信息,利用分形理论确定临界值进行分级减少了这些信息的影响。本次研究通过门限法和分形方法两种划分方法对研究区的蚀变异常进行对比,最后根据研究区蚀变异常信息的分形特征,利用求和方法对研究区的铁染和泥化蚀变异常信息进行分级。结果表明,相对于门限法,在遥感蚀变信息提取中分形方法加强了阈值选取的准确度,并发现研究区泥化蚀变与铁染蚀变主要分布于北东部及北部区域,其它区域小面积离散分布,这可为矿产勘查工作提供更好的技术支撑。

关键词：蚀变异常遥感解译分形理论主成分分析法门限法广东阳山县

Targeting the nitric oxide/cGMP signaling pathway to treat chronic pain

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2023年第5期18卷 996-1003页

作者： dan-Yang li Shao-Jie Gao Jia Sun Long-Qing Zhang Jia-Yi Wu Fan-He Song Dai-Qiang liu Ya-Qun Zhou Wei Mei Department of Anesthesiology and Pain Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Nitric oxide(NO)/cyclic guanosine 3′,5′-monophosphate(cGMP) signaling has been shown to act as a mediator involved in pain transmission and processing. In this review, we summarize and discuss the mechanisms of the NO/cGMP signaling pathway involved in chronic pain, including neuropathic pain, bone cancer pain, inflammatory pain, and morphine tolerance. The main process in the NO/cGMP signaling pathway in cells involves NO activating soluble guanylate cyclase, which leads to subsequent production of cGMP. cGMP then activates cGMP-dependent protein kinase(PKG), resulting in the activation of multiple targets such as the opening of ATP-sensitive K+ channels. The activation of NO/cGMP signaling in the spinal cord evidently induces upregulation of downstream molecules, as well as reactive astrogliosis and microglial polarization which participate in the process of chronic pain. In dorsal root ganglion neurons, natriuretic peptide binds to particulate guanylyl cyclase, generating and further activating the cGMP/PKG pathway, and it also contributes to the development of chronic pain. Upregulation of multiple receptors is involved in activation of the NO/cGMP signaling pathway in various pain models. Notably the NO/cGMP signaling pathway induces expression of downstream effectors, exerting both algesic and analgesic effects in neuropathic pain and inflammatory pain. These findings suggest that activation of NO/cGMP signaling plays a constituent role in the development of chronic pain, and this signaling pathway with dual effects is an interesting and promising target for chronic pain therapy.

关键词： bone cancer pain chronic pain cyclic GMP dorsal root ganglion inflammatory pain morphine tolerance neuropathic pain nitric oxide protein kinase G spinal cord

Transcriptional landscape of rice roots at the single-cell resolution

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2021年第3期14卷 384-394页

作者： Qing liu Zhe liang dan Feng Sanjie Jiang Yifan Wang Zhuoying Du Ruoxi li Guihua Hu Pingxian Zhang Yanfei Ma Jan ULohmann Xiaofeng Gu Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Centre for Organismal Studies Heidelberg UniversityHeidelberg 69120Germany BGI Genomics Shenzhen 518083China Fu Foundation School of Engineering and Applied Science Columbia UniversityNew YorkNY 10027USA These authors contributed equally.

There are two main types of root systems in flowering plants,namely taproot systems of dicots and fibrous root systems found in *** this fundamental split,our current knowledge of cellular and molecular mechanism driving root development is mainly based on studies of the dicot model ***,the world major crops are monocots and little is known about the transcriptional programs underlying cell-type specification in this ***,we report the transcriptomes of more than 20000 single cells derived from root tips of two agronomically important rice *** combined computational and experimental analyses we were able to robustly identify most of the major cell types and define novel cell-type-specific marker genes for both ***,we found divergent cell types associated with specific regulatory programs,including phytohormone biosynthesis,signaling,and response,which were well conserved between the two rice *** addition,we detected substantial differences between the cell-type transcript profiles of Arabidopsis and *** species-specific features emphasize the importance of analyzing tissues across diverse model species,including *** together,our study provides insight into the transcriptomic landscape of major cell types of rice root tip at single-cell resolution and opens new avenues to study cell-type specification,function,and evolution in plants.

关键词： single-cell RNA-seq transcriptional landscape rice root plants

Baculovirus Surface Display of Zika Virus Envelope Protein Protects against Virus Challenge in Mouse Model

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第5期35卷 637-650页

作者： dan Luo Yuanjiu Miao Xianliang Ke Zhongyuan Tan Chun Hu Penghui li Ting Wang Yuan Zhang Jianhong Sun Yan liu Hanzhong Wang Zhenhua Zheng CAS Key Laboratory of Special Pathogens and Biosafety Center for Emerging Infectious DiseasesWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Computer Center of Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Wuhan Institute of Physics and Mathematics Chinese Academy of SciencesWuhan 430071China

Zika virus(ZIKV)is emerging as a significant pathogen worldwide and may cause severe neurological disorders such as fetal microcephaly and Guillain-Barre *** drug or listed vaccines are currently available for preventing ZIKV *** a major target of neutralizing,ZIKV envelop(E)protein usually used for vaccine ***,the immunogenicity of ZIKV envelop(E)protein expressed by baculovirus display system has never been *** this study,we reported a new strategy for surface display of ZIKV E protein by a recombinant baculovirus vector derived from Autographa californica multiple nuclear polyhedrosis virus(AcMNPV)and assessed its immunogenicity in *** produced recombinant fusion ZIKV E protein linked with signal peptide(SP)and transmembrane domain(TM)of AcMNPV *** results showed that the recombinant protein was easy to produce by baculovirus display ***/c mice immunized with this recombinant E protein developed ZIKV specific serum *** anti-E protein sera from the mice were able to effectively neutralize ZIKV in *** importantly,AG6(IFN-a/b and IFN-c receptor deficient)mice immunized with recombinant E protein were protected against lethal ZIKV ***,thesefindings demonstrated that the recombinant E protein displayed by baculovirus can be conveniently prepared and displayed good immunogenicity in immunized *** is a promising practical approach for prompting the development of vaccine and related immunology research.

关键词： Zika virus(ZIKV) Envelope protein Baculovirus Immunogenicity Neutralizing antibody Viral challenge