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Neural Regeneration Research 2020年第5期15卷 880-886页

作者： dan lyu Ning Tang Andrew W. Womack Yong-Jin He Qing Lin Department of Pain Management Tianjin First Center HospitalTianjinChina Department of Psychology College of ScienceUniversity of Texas at ArlingtonArlingtonTXUSA Reproductive Medicine Center the 960^th Hospital of the PLA Joint Logistics Support ForceJinanShandong ProvinceChina

Ketamine exposure can lead to selective neuroapoptosis in the developing brain.p66ShcA,the cellular adapter protein expressed selectively in immature neurons,is a known pro-apoptotic molecule that triggers neuroapoptosis when ***-Dawley rats at postnatal day 7 were subcutaneously injected in the neck with ketamine 20 mg/kg,six times at 2-hour *** 0,1,3,and 6 hours after final injection,western blot assay was used to detect the expression of cleaved caspase-3,p66ShcA,and phosphorylated *** found that the expression of activated p66ShcA and caspase-3 increased after ketamine exposure and peaked at 3 *** same procedure was performed on a different group of *** the age of 4 weeks,spatial learning and memory abilities were tested with the Morris water *** to find the hidden platform for these rats was longer than it was for control rats,although the residence time in the target quadrant was *** findings indicate that ketamine exposure resulted in p66ShcA being activated in the course of an apoptotic cascade during the neonatal *** may have contributed to the deficit in spatial learning and memory that persisted into *** experimental protocol was approved by the Institutional Animal Care and Use Committee at the University of Texas at Arlington,USA (approval No.A13.008) on January 22,2013.

关键词： caspase-3 developmental neuroapoptosis hippocampus ketamine Morris water maze N-methyl-D-aspartate acid receptors p66ShcA spatial learning

Clinical value of next-generation sequencing in guiding decisions regarding endocrine therapy for advanced HR-positive/HER-2-negative breast cancer

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Chinese Journal of Cancer Research 2022年第4期34卷 343-352页

作者： dan lyu Binliang Liu Bo Lan Xiaoying Sun Lixi Li Jingtong Zhai Haili Qian Fei Ma Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Breast Cancer Medical Oncology Hunan Cancer Hospital/the Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityChangsha 410013China Department of Medical Oncology Cancer Hospital of Huanxing Chaoyang DistrictBeijing 100122China State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Objective: The mechanism of acquired gene mutation plays a major role in resistance to endocrine therapy in hormone receptor(HR)-positive advanced breast cancer. Circulating tumor DNA(ctDNA) has been allowed for the assessment of the genomic profiles of patients with advanced cancer. We performed this study to search for molecular markers of endocrine therapy efficacy and to explore the clinical value of ctDNA to guide precise endocrine therapy for HR-positive/human epidermal growth factor receptor-2(HER-2)-negative metastatic breast cancer ***: In this open-label, multicohort, prospective study, patients were assigned to four parallel cohorts and matched according to mutations identified in ctDNA: 1) activation of the phosphatidylinositol-3-kinase(PI3K)/AKT/mammalian target of rapamycin(mTOR) signaling pathway preferred mTOR inhibitor combined with endocrine therapy;2) estrogen receptor 1(ESR1) mutation preferred fulvestrant;3) HER-2 mutations preferred pyrotinib;and 4) no actionable mutations received treatment according to the clinical situation. In all cohorts, patients were divided into compliance group and violation group. The primary outcome measure was progression-free survival(PFS), and the secondary outcome measure was overall survival(OS).Results: In all cohorts, the combined median PFS was 4.9 months, and median PFS for the compliance and violation groups was 6.0 and 3.0 months, respectively [P=0.022, hazard ratio(HR)=0.57]. Multivariate Cox regression model showed the risk of disease progression was lower in compliance group than in violation group(P=0.023, HR=0.55). Among the patients with HER-2 mutations, the median PFS was 11.1 months in the compliance group and 2.2 months in the violation group(P=0.011, HR=0.20). There was no significant difference in the median PFS between patients who did and did not comply with the treatment protocol in patients with activation of the PI3K/AKT/mTOR or ESR1 ***: The results suggest th

关键词： ctDNA next-generation sequencing breast cancer endocrine therapy

Two-dimensional MoS_(2)/diamond based heterojunctions for excellent optoelectronic devices:current situation and new perspectives

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Rare Metals 2023年第10期42卷 3201-3211页

作者： Liang-Rui Zou Xiao-dan lyu dan-dan Sang Yu Yao Shun-Hao Ge Xue-Ting Wang Chuan-Dong Zhou Hai-Long Fu Hong-Zhu Xi Jian-Chao Fan Cong Wang Qing-Lin Wang Shandong Key Laboratory of Optical Communication Science and Technology School of Physics Science and Information TechnologyLiaocheng UniversityLiaocheng252000China College of Mathematics and Physics Beijing University of Chemical TechnologyBeijing100029China Division of Physics and Applied Physics School of Physical and Mathematical SciencesNanyang Technological UniversitySingapore637371Singapore Shandong Liaocheng Laixin Powder Materials Science and Technology Co. Ltd.Liaocheng252000China Zhejiang Province Key Laboratory of Quantum Technology and Device Department of PhysicsZhejiang UniversityHangzhou310027China The Institute of Anhui Huadong Photoelectric Technology Wuhu241002China

Two-dimensional(2D)semiconductor molybdenum disulfide(MoS_(2))can be used as n-channel and is considered as a key candidate material to advance the promising development of optoelectronic *** high thermal conductivity,breakdown voltage,carrier mobility,and high saturation velocity of diamond offer the possibility of making it high-frequency device material in hightemperature and high-power *** addition of 2D MoS_(2)nanolayers and nanosheets to diamond thin film to form heterojunction can improve the carrier transport performance of the optoelectronic device in harsh *** this perspective,the prospects of 2D MoS_(2)/diamond heterojunction for challenges and new designs of optoelectronic applications are discussed,including photodetectors,memories,transistors,light emission diodes,and electron field emission devices to further explore the development of 2D material device field in complex environments.

关键词： MoS_(2) Optoelectronics Heterojunction Diamond Extreme environment

Focus on anticancer therapy-induced cardiotoxicity from the perspective of oncologists

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Chinese Medical Journal 2022年第17期135卷 2122-2124页

作者： Binliang Liu Yanfeng Wang dan lyu Fei Ma Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Breast Cancer Medical Oncology Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya Medical SchoolCentral South UniversityChangshaHunan 410013China Department of Comprehensive Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

To the Editor:Cancer and cardiovascular(CV)disease are associated with the largest morbidity and mortality in the world,and they are related to each other through some common risk factors.

关键词： mortality cardiovascular Cancer

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上排渣式全断面竖井掘进机凿井技术与应用

隧道建设（中英文） 2023年第1期43卷 151-160页

作者：吕旦贾连辉中铁工程装备集团有限公司河南郑州450016

为验证全断面竖井掘进机凿井技术的可靠性,依托宁海抽水蓄能排风竖井工程,解析上排渣式全断面竖井掘进机上排渣系统、开挖系统、姿态调整系统及井下动态实时监控系统等关键系统的设计及应用。应用表明:1)在井壁上布置环向导水槽和优化... 详细信息

为验证全断面竖井掘进机凿井技术的可靠性,依托宁海抽水蓄能排风竖井工程,解析上排渣式全断面竖井掘进机上排渣系统、开挖系统、姿态调整系统及井下动态实时监控系统等关键系统的设计及应用。应用表明:1)在井壁上布置环向导水槽和优化掘进排渣参数,保证了竖井掘进机在凝灰岩含水地层的顺利通过;2)采用前装刀设计满足使用要求,通过刀盘支地系统和刀具更换工装,实现了竖井掘进机前装刀的安全更换;3)采用竖井掘进机独特的姿态调整方法,满足了井筒掘进的精度要求;4)基于渣土称重和热成像原理,解决了粉尘环境下吊桶装渣的可视化作业;5)采用基于UWB技术的竖井掘进机分层平面定位系统,实现了竖井作业区域内人员的实时定位。

关键词：全断面竖井掘进机凝灰岩上排渣系统刀具更换姿态调整实时监控

小鼠抗人FOXO3保守区多克隆抗体的制备和鉴定

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细胞与分子免疫学杂志 2017年第6期33卷 838-844页

作者：李蕾吕丹赤峰学院附属医院病理科赤峰学院医学院病理生理学教研室内蒙古赤峰024000 北京大学医学部系统生物医学研究所北京100191

目的纯化叉头盒蛋白O3(FOXO3)基因保守区蛋白,并制备其多克隆抗体。方法利用生物信息学分析预测FOXO3蛋白的保守区,通过PCR扩增编码FOXO3蛋白第290~472位氨基酸DNA片段并克隆至pET28a原核表达载体中,转化到大肠杆菌BL21,诱导表达并获得... 详细信息

目的纯化叉头盒蛋白O3(FOXO3)基因保守区蛋白,并制备其多克隆抗体。方法利用生物信息学分析预测FOXO3蛋白的保守区,通过PCR扩增编码FOXO3蛋白第290~472位氨基酸DNA片段并克隆至pET28a原核表达载体中,转化到大肠杆菌BL21,诱导表达并获得可溶性蛋白,经镍离子柱亲和纯化,免疫小鼠制备抗血清,随后采用ELISA、Western blot法和免疫沉淀实验检测抗体的效价、特异性以及应用范围。结果成功构建表达载体pET28a-FOXO3(aa290-472),实现可溶性FOXO3蛋白的表达和抗体制备。SDS-PAGE和Western blot实验结果证实,FOXO3蛋白与预期结果一致,抗血清能够特异性地识别原核、真核FOXO3蛋白以及其天然抗原表位。结论成功制备了小鼠抗人FOXO3的多克隆抗体,且抗体对内源性FOXO3具有较好的反应性和特异性。

关键词：叉头盒蛋白O3(FOXO3) 多克隆抗体融合蛋白

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沉井掘进机关键技术研究与试验

隧道建设（中英文） 2023年第S2期43卷 578-586页

作者：赵飞徐光亿吕旦中铁工程装备集团有限公司河南郑州450000

为解决传统沉井工法施工效率低、地质适应性差的问题,提出一种机械法开挖的沉井掘进机,即采用截割滚筒开挖岩土、抓斗或泥浆泵出渣。通过提压装置将预制管片拼装成的井筒有控制地下沉,掘排同步施工实现一次性成井。系统介绍沉井掘进机... 详细信息

为解决传统沉井工法施工效率低、地质适应性差的问题,提出一种机械法开挖的沉井掘进机,即采用截割滚筒开挖岩土、抓斗或泥浆泵出渣。通过提压装置将预制管片拼装成的井筒有控制地下沉,掘排同步施工实现一次性成井。系统介绍沉井掘进机的工作原理、系统构成和主机参数,并阐述井筒提压纠偏、自由断面开挖轮廓控制等关键技术,总结出一套与掘进机相匹配的施工工艺。最后通过模拟不同工况和地质情况,对整机进行全工序的功能性试验验证,试验结果证明沉井掘进机的整机性能满足设计要求。

关键词：沉井掘进机试验验证截割滚筒管片提压装置

宜宾市0-3岁儿童维生素D营养状况及其与监护人营养知识态度行为的关系

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中国儿童保健杂志 2024年第4期32卷 445-450页

作者：田雪王琳吕丹宜宾市第一人民医院普儿科四川宜宾644000

目的探讨宜宾市0~3岁儿童血清维生素D水平与其监护人营养知识态度行为(KAP)的相关性,为预防儿童维生素D缺乏提供参考。方法选取2020年1-12月于宜宾市第一人民医院进行儿童保健且检测血清25-(OH)D的783名0~3岁儿童为研究对象,收集所有儿... 详细信息

目的探讨宜宾市0~3岁儿童血清维生素D水平与其监护人营养知识态度行为(KAP)的相关性,为预防儿童维生素D缺乏提供参考。方法选取2020年1-12月于宜宾市第一人民医院进行儿童保健且检测血清25-(OH)D的783名0~3岁儿童为研究对象,收集所有儿童一般情况(喂养情况、生长发育情况、生活方式等),采集静脉血用化学发光法测定血清25-(OH)D水平,使用自行设计的喂养知识-态度-行为问卷调查其监护人KAP,采用Pearson相关和关联性分析儿童维生素D营养状况与监护人营养KAP的关系。结果1)0~3岁儿童纯母乳喂养率为62.2%,不同年龄段儿童的每日户外活动时间、维生素D平均补充量差异有统计学意义(t=9.030、10.260,P<0.05)。2)血清25-(OH)D浓度平均为(36.8±8.7)ng/mL,维生素D缺乏率为21.84%,不同年龄段、体型、户外活动时间及维生素D制剂补充情况的儿童血清25-(OH)D水平及维生素D缺乏率差异有统计学意义(t/F:2.220~6.302,χ^(2):5.346~33.134,P<0.05)。3)监护人营养KAP得分分别为(78.9±9.9)、(88.1±8.3)分和(78.3±11.8)分,父母KAP得分高于其他监护人(P<0.05)。4)血清25-(OH)D水平与监护人营养知识、态度和行为评分均呈正相关(r=0.805、0.650、0.831,P<0.05),监护人营养KAP分级和维生素D缺乏有关联性(P<0.05)。5)体型超重或肥胖是维生素D缺乏的危险因素(OR=2.126,95%CI:1.162~3.887),每日户外活动时间≥2h(OR=0.592,95%CI:0.392~0.895)、规律补充维生素D(OR=0.618,95%CI:0.456~0.838)和监护人营养行为良好(OR=0.725,95%CI:0.563~0.933)是儿童维生素D缺乏的保护因素(P<0.05)。结论宜宾市0~3岁儿童维生素D缺乏率高,维生素D营养状况与监护人营养KAP有关,提高监护人营养KAP有利于预防儿童维生素D缺乏。

关键词：维生素D 儿童知识态度行为监护人

C19ORF12对胃癌MKN45细胞增殖和化疗药敏感性的影响及其机制

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吉林大学学报（医学版） 2021年第3期47卷 687-693页

作者：杨颖赵伟吕丹北京大学人民医院输血科北京100044 中日友好医院检验科北京100029 北京大学医学部系统生物医学研究所北京100191

目的:探讨19号染色体开放读码框12(C19ORF12)对胃癌MKN45细胞增殖和化疗药物敏感性的影响,并阐明其作用机制。方法:将胃癌MKN45细胞分为对照组和C19ORF12组。CCK-8法检测2组胃癌MKN45细胞的增殖活性,克隆形成实验检测2组胃癌MKN45细胞... 详细信息

目的:探讨19号染色体开放读码框12(C19ORF12)对胃癌MKN45细胞增殖和化疗药物敏感性的影响,并阐明其作用机制。方法:将胃癌MKN45细胞分为对照组和C19ORF12组。CCK-8法检测2组胃癌MKN45细胞的增殖活性,克隆形成实验检测2组胃癌MKN45细胞克隆形成数,CCK-8法检测加入化疗药物阿霉素或过氧化氢(H2O2)后2各组胃癌MKN45细胞存活率,流式细胞术检测2组胃癌MKN45细胞凋亡率,激光共聚焦显微镜技术检测C19ORF12在胃癌MKN45细胞中的亚细胞定位,GEPIA分析癌症基因组图谱(TCGA)中C19ORF12 mRNA在正常胃组织和胃癌组织中的表达差异,KM-plot回顾性分析不同表达水平C19ORF12对胃癌患者预后的影响。结果:与对照组比较,C19ORF12组胃癌MKN45细胞增殖活性明显升高(P<0.05),克隆形成数升高(P<0.01)。阿霉素或H2O2作用后,与对照组比较,C19ORF12组胃癌MKN45细胞存活率升高(P<0.05或P<0.01),细胞凋亡率降低(P<0.01)。激光共聚焦显微镜下,C19ORF12主要定位于胃癌MKN45细胞的线粒体中。与正常胃组织比较,胃癌组织中C19ORF12 mRNA表达水平明显升高(P<0.01)。与低表达C19ORF12的胃癌患者比较,高表达C19ORF12的胃癌患者总体生存期明显缩短(P<0.01)。结论:C19ORF12能够促进胃癌细胞的增殖,并且降低胃癌细胞对化疗药物的敏感性。C19ORF12可以作为肿瘤治疗的潜在靶标。

关键词： C19ORF12 胃肿瘤细胞增殖阿霉素化疗药敏感性活性氧

超疏水低红外发射率复合涂层的制备及性能表征

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表面技术 2020年第7期49卷 105-111页

作者：张伟钢郑梦影吕丹丹滁州学院安徽滁州239000

目的制备得到具有超疏水特性的低红外发射率涂层。方法以纳米SiO_2为微纳结构改性剂,聚二甲基硅氧烷(PDMS)为树脂基体,Al粉为功能颜料,采用刮涂法制备得到了多种类型的改性和非改性PDMS/Al复合涂层。分析探讨了PDMS和Al粉配比、纳米SiO_... 详细信息

目的制备得到具有超疏水特性的低红外发射率涂层。方法以纳米SiO_2为微纳结构改性剂,聚二甲基硅氧烷(PDMS)为树脂基体,Al粉为功能颜料,采用刮涂法制备得到了多种类型的改性和非改性PDMS/Al复合涂层。分析探讨了PDMS和Al粉配比、纳米SiO_2添加量及表面微纳结构层对涂层性能的影响规律及成因。结果 PDMS和Al粉配比对涂层发射率和光泽度影响明显,当PDMS和Al粉配比为6∶4时,涂层的发射率和光泽度可分别低至0.265和10.3,涂层的水接触角可达到109.5°,要明显高于传统树脂基低发射率涂层。添加纳米SiO_2可使涂层的粗糙度上升明显,从而使涂层的疏水性显著增强。当纳米SiO_2质量分数为8%时,涂层的水接触角可增大到130°,涂层的发射率和光泽度仍可低至0.330和9.8。PDMS/SiO_2微纳结构层可在PDMS/Al复合涂层表面形成明显的乳突状微纳结构,从而使涂层实现超疏水特性。当PDMS和纳米SiO_2配比为7∶3时,改性后的涂层水接触角可高达156°,同时具有相对较低的发射率(0.661)和极低的光泽度(2.1)。结论在PDMS/Al复合涂层表面构筑一定厚度的微纳结构层,可实现该涂层超疏水、低发射率和低光泽的兼容。

关键词：复合涂层纳米SiO2 红外发射率光泽度水接触角