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Influence of epigallocatechin gallate on the immune function of dendritic cells after ultraviolet B irradiation

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Journal of Microbiology and Immunology 2007年第2期5卷 90-98页

作者： dan LUO, bing RONG ZHOU XIJI Department of Dermatology the First Affiliated Hospital of Nanjing Medical University Nanjing P. R.China

To investigate the protective effect of epigallocatechin gallate （EGCG） on the immune function of dendritic cells （DCs） after ultraviolet B irradiation （UVB） and its underlying mechanisms, the monocytes were isolated from peripheral blood and cultivated into DCs with cytokines, such as GM-CSF and IL-4. DCs were harvested after cultivation for 7 d and subjected to irradiation with different dosages of UVB. Then, 200 μg/ml of EGCG were added in certain groups immediately after irradiation. DCs simply treated with UVB or treated with both UVB and EGCG were co-cultured with lymphocytes, and MTT assay was used to detect the ability of DCs to stimulate proliferation of lymphocytes. Surface markers CDS0, CD86, HLA-DR and CD40 were detected by flow cytometry, and the levels of IL-10 and IL-12 secreted from DCs 2d h after cultivation were measured by ELISA. It was demonstrated that UVB irradiation could inhibit the ability of DCs to stimulate the proliferation of lymphocytes and surface expressions of CDS0, CD86, HLA-DR and CD40 on DCs in a dose-dependent manner. The inhibition rate of DCs was improved to some extent after treatment with 200μg/ml of EGCG. When the concentra- tion of EGCG exceeded 100 μg/ml, the enhancing effect of EGCG on the expression of the co-stimulating molecules on DCs could be demonstrated in a dose-dependent manner. UVB showed no significant influence on the secretion of IL-10 and IL-12 from DCs, while EGCG could down-regulate the secretion level of IL-12 and up-regulate that of IL-10. It is concluded that EGCG can antagonize the inhibitory effect on DCs induced by UVB irradiation. This function has some relationship with its protecting effect of the expression of the co-stimulating molecule on the surface of DCs and the secretion level of IL-10 and IL-12.

关键词： Epigalloeatechin gallate Ultraviolet B Dendritic cells

Expression ofα2-Na/K-ATPase in C57BL/6J Mice Inner Ear and Its Relationship with Age-related Hearing Loss

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Current Medical Science 2021年第1期41卷 153-157页

作者： Yun LIU Han-qi CHU Yan-bo SUN dan bing Liang-qiang ZHOU Jin CHEN Qing-guo CHEN Zhi-hui DU Department of Otolaryngology-Head and Neck Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Otolaryngology-Head and Neck Surgery the Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

K^(+)cycling in the cochlea is critical to maintain *** sodium-potassium pumps are proved to participate in K^(+)cycling,such as Na/***α2-Na/K-ATPase is an important isoform of Na/*** expression ofα2-Na/K-ATPase in the cochlea is not *** this study,we used C57BL/6 mice as a model of presbycusis and implemented immunohistochemistry staining and quantitative real time-PCR,and theα2-Na/K-ATPase expression pattern was confirmed in the inner *** was foundα2-Na/K-ATPase was expressed widely in cochlea and its mRNA and protein expression was gradually reduced with aging(4-,14-,26-and 48-weeks old mice).We suspected that,the down-regulation ofα2-Na/K-ATPase expression might be associated with the remodeling of K^(+)cycling,degeneration of morphological structure and decrease of hearing function in aging C57 *** conclusion,we speculated that the reduction ofα2-Na/K-ATPase might play an important role in the pathogenesis of age-related hearing loss.

关键词： α2-Na/K-ATPase C57BL/6J mouse inner ear age-related hearing loss K^(+)cycling

Thickness-dependent excitonic properties of atomically thin 2H-MoTe2

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Chinese Physics B 2020年第1期29卷 452-456页

作者： Jin-Huan Li dan bing Zhang-Ting Wu Guo-Qing Wu Jing Bai Ru-Xia Du Zheng-Qing Qi Department of Basic Teaching Nanjing Tech University Pujiang InstituteNanjing 211134China School of Network and Communication Engineering Jinling Institute of TechnologyNanjing 211169China Lab for Nanoelectronics and NanoDevices Department of Electronics Science and TechnologyHangzhou Dianzi UniversityHangzhou 310018China

Two-dimensional(2D)2H-MoTe2 is a promising semiconductor because of its small bandgap,strong absorption,and low thermal *** this paper,we systematically study the optical and excitonic properties of atomically thin 2H-MoTe2(1–5 layers).Due to the fact that the optical contrast and Raman spectra of 2H-MoTe2 with different thicknesses exhibit distinctly different behaviors,we establish a quantitative method by using optical images and Raman spectra to directly identify the layers of 2H-MoTe2 thin ***,excitonic states and binding energy in monolayer/bilayer 2H-MoTe2 are measured by temperature-dependent photoluminescence(PL)*** temperature T=3.3 K,we can observe an exciton emission at^1.19 eV and trion emission at^1.16 eV for monolayer *** at room temperature,the exciton emission and trion emission both disappear for their small binding *** determine the exciton binding energy to be 185 meV(179 meV),trion binding energy to be 20 meV(18 me V)for the monolayer(bilayer)*** thoroughly studies of the excitonic states in atomically thin 2H-MoTe2 will provide guidance for future practical applications.

关键词： 2H-MoTe2 photoluminescence Raman exciton and trion

Comparisons of Supine Roll Test and Alternative Positional Tests in HC-BPPV Lateralization

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Current Medical Science 2022年第3期42卷 613-619页

作者： Geeta KOJU dan bing Liang-qiang ZHOU Zhong-yao MAO Yan-ling TAO Qing-guo CHEN Jin CHEN Yun LIU Ting TONG Han-qi CHU Department of Otolaryngology-Head and Neck Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective The purpose of the study was to evaluate the efficiency of the supine roll test(SRT)and alternative positional tests(APTs)including the bow and lean test(BLT),pseudo-spontaneous nystagmus(PSN),and lying down nystagmus(LDN)to identify the affected side in horizontal canal benign paroxysmal positional vertigo(HC-BPPV).Methods In our prospective study,we performed a testing profile(PSN,BLT,LDN,SRT)on 59 HC-BPPV patients using *** compared the accuracy and sensitivity of these tests in HC-BPPV *** from 30 healthy patients were collected as the control *** When performing positional tests,the elicited nystagmus coinciding with Ewald’s second law was defined as a“positive response”.In 44 patients with geotropic nystagmus,the rates of positive response in LDN,PSN,and BLT were 22/44(50%),19/44(43%),and 18/44(41%),respectively,while in 15 patients with apogeotropic nystagmus,the positive response rates of these three tests were 10/15(66.7%),9/15(60%),and 4/15(27.00%),*** sensitivity of LDN(54.38%)was higher than that of PSN(47.37%)and BLT(38.60%)but lower than that of SRT(89.47%).Notably,the accuracy rate of PSN(71.8%)was higher than that of the other *** 6 patients with symmetrical nysgtamus during the roll test,5 patients showed a positive response in both LDN and BLT(83.34%),whereas 4 patients showed a positive response in PSN(66.67%).Conclusion All positional tests are helpful for determining the affected side of HC-BPPV,but SRT carries the highest accuracy of lateralization followed by PSN.

关键词： horizontal canal benign paroxysmal positional vertigo pseudo spontaneous nystagmus bow and lean test lying down nystagmus supine roll test

Functional protection of pentoxifylline against spinal cord ischemia/reperfusion injury in rabbits： necrosis and apoptosis effects

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Chinese Medical Journal 2008年第23期121卷 2444-2449页

作者： ZHU dan-jie XIA bing BI Qing ZHANG Shui-jun QIU Bin-song ZHAO Chen Department of Orthopedics Zhejiang Provincial People's Hospital Hangzhou Zhejiang 310014 China

Background Little is known about neuronal death mechanisms following spinal cord ischemia. The present study aimed to investigate the protective effect of pentoxifylline （PTX） against spinal cord ischemia/reperfusion （I/R） injury. Methods Rabbits sustained spinal cord ischemia following 45 minutes cross-clamping of the infrarenal aorta. Experimental groups were as follows： the first group of animals （sham, n=-8） underwent laparotomy alone and served as the sham group; the second group （I/R, n=20） received carrier （3 ml saline solution） and served as the control group; the third group （PTX-A, n=20） received PTX intravenously 10 minutes prior to ischemia; and the fourth group （PTX-B, n=20） received PTX intravenously at the onset of reperfusion. Rabbits were evaluated for hind-limb motor function with the Tarlov scoring system at 48 hours. Serum was assayed with enzyme-linked immunosorbent assay for tumor necrosis factor α （TNF-α） and spinal cords were harvested for myeloperoxidase （MPO） activity, histopathological analysis, terminal deoxynucleotidyl transferase （TdT）-rnediated dUTP nick end labeling staining, platelet/endothelial ceU adhesion molecule-1 （PECAM-1） and caspase-3 immunohistochemistry, and the number of necrotic and apoptotic neuron were counted and data analyzed at 12, 24, 48 and 72 hours of reperfusion. Spinal cords were studied by electron microscopy. Results Improved Tarlov scores were seen in PTX-treated rabbits as compared with ischemic control rabbits at 48 hours. A significant reduction was found in TNF-α in serum, activity of MPO and immunoreactivity of the PECAM-1 and caspase-3 in PTX-treated rabbits. There were fewer apoptotic neurons than necrotic neurons （P 〈0.05）. A significant decrease in both necrotic and apoptotic neurons was observed in the PTX-treated groups （PTX-A and PTX-B） compared with the I/R group （P 〈0.05）. Both necrotic and apoptotic neurons were found with the electron microscope. Conclusions PTX may induce protection aga

关键词： spinal cord injury pentoxifylline necrosis apoptosis

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股权结构与农村商业银行二元绩效研究

农业经济问题 2018年第2期39卷 60-70页

作者：刘丹张兵南京农业大学金融学院

本文基于农村商业银行服务＂三农＂的特殊性,利用2012—2015年的调查数据理论与实证分析了股权结构对农村商业银行社会绩效和财务绩效的影响。研究发现：在股东类型方面,高管持股、员工持股和银行持股对农村商业银行的社会绩效影响不显... 详细信息

本文基于农村商业银行服务＂三农＂的特殊性,利用2012—2015年的调查数据理论与实证分析了股权结构对农村商业银行社会绩效和财务绩效的影响。研究发现：在股东类型方面,高管持股、员工持股和银行持股对农村商业银行的社会绩效影响不显著,但均对其财务绩效影响显著;政府性质股东对农村商业银行社会绩效影响显著,对其财务绩效显著负向影响。在股权集中度方面,高管股权集中度越高,农村商业银行的财务绩效越优异;非金融法人股权集中度越高,将不利于农村商业银行实现良好的财务绩效和社会绩效;政府性质股东股权集中度越高,农村商业银行的社会绩效表现越好,但不利于财务绩效的提升。

关键词：农村商业银行股权结构社会绩效影响机理

Expression Patterns and Implications of LaminBl in Rat Cochleae

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Current Medical Science 2019年第2期39卷 305-309页

作者： Zhi-hui DU Jin CHEN Qing-guo CHEN Liang-qiang ZHOU dan bing Yun LIU Yan-bo SUN Peng-jun LI Fan QI Hong-mei ZHU Han-qi CHU Department of Otolaryngology-Head and Neck Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

LaminB1,a major component of the nuclear lamina,is a potent regulator of cellular proliferation and senescence and also known to be essential for neuronal migration and brain ***,the expression patterns of LaminB1 in the rat cochleae are still not fully *** *** blotting,and quantitative real-time PCR,we identified the distribution and expression of LaminB1 in the rat *** staining indicated that LaminBl was mainly localized in the auditory hair cells(HCs),spiral ganglion cells(SGC),stria vascularis(STV,including spiral ligament),Reissner's membrane(RM),and limbus laminae spiralis(LLS).Western blotting analysis illustrated that the distribution of LaminBl in rat cochleae was characterized by tissue *** LaminB1 protein was expressed more in SGC and basilar membrane(BM)than in ***,the mRNA expression of LaminB1 displayed difference in cochlear *** observations preliminarily revealed the expression patterns of LaminB1,providing a theoretical basis for further study on the role of LaminB1 in auditory function.

关键词： LaminB1 cochleae rat hair cell spiral ganglion cell stria vascularis

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传统知识界定的困惑与解析

西北大学学报（哲学社会科学版） 2016年第4期46卷 112-117页

作者：刘丹冰西北大学法学院陕西西安710127

术语或概念是构建法律制度的起点,因此,界定传统知识就成为传统知识保护的前提。由于不同国家、国际组织对传统知识的理解不同,传统知识的界定仍然存在难度。界定传统知识首先应关注其所蕴含的商业价值,准确理解"属于传统"和"基于传统"... 详细信息

术语或概念是构建法律制度的起点,因此,界定传统知识就成为传统知识保护的前提。由于不同国家、国际组织对传统知识的理解不同,传统知识的界定仍然存在难度。界定传统知识首先应关注其所蕴含的商业价值,准确理解"属于传统"和"基于传统",并明确集体才是传统知识的绝对主体,产权是传统知识保护的核心。

关键词：生物多样性公约惠益分享世代相传亚马孙灵蔓专利纠纷

我国中央银行流动性工具箱的创新、演进特点及法律问题研究

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西北大学学报（哲学社会科学版） 2022年第1期52卷 93-103页

作者：刘丹冰西北大学法学院陕西西安710127

对金融市场流动性予以调控,是中央银行的法定职责。单一货币政策体系下,存款准备金率、基准利率、公开市场业务是我国中央银行流动性工具箱的三大工具;而货币政策与宏观审慎双支柱体系下,则需要在原有流动性工具箱改进的基础上进一步创... 详细信息

对金融市场流动性予以调控,是中央银行的法定职责。单一货币政策体系下,存款准备金率、基准利率、公开市场业务是我国中央银行流动性工具箱的三大工具;而货币政策与宏观审慎双支柱体系下,则需要在原有流动性工具箱改进的基础上进一步创新。由此,常备借贷便利、中期借贷便利等登上历史舞台。梳理我国中央银行流动性工具箱的创新演进过程,发现流动性调控中存在中国人民银行法律地位不够明确、流动性工具取舍中的法律依据不足、中央银行流动性调控中自由裁量权的边界不清等法律问题,这些均是未来修订、制定法律应予以重视的。只有解决了这些问题,我国中央银行对流动性的调控才能在法律界限内进行,市场的透明度与公平才能强化。

关键词：存款准备金率存贷款基准利率常备借贷便利中央银行法