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American Journal of Plant Sciences 2015年第5期6卷 666-675页

作者： Lucilene Fernandes Silva Maria das Gracas Cardoso Luís Roberto Batista Marcos de Souza Gomes Leonardo Milani Avelar Rodrigues danúbia A.de carvalho selvati rezende Maria Luisa Teixeira Marcos Schleiden Sousa carvalho Juliana de Andrade Santiago David Lee Nelson department of Chemistry Federal University of Lavras Lavras Brazil department of Food Science Federal University of Lavras Lavras Brazil department of Agriculture Federal University of Lavras Lavras Brazil Federal University of Vales de Jequitinhonha e Mucuri Diamantina Brazil

The essential oils from Mentha viridis L. and Mentha pulegium L. were characterized by gas chromatography coupled to mass spectrometry (GC-MS). These oils were obtained by hydrodistillation and presented linalool (40.70%), carvone (13.52%) and α-terpinene (8.56%) as the principal constituents in the essential oil from Mentha viridis L. Pulegone (50.01%), menthol (31.90%) and menthone (16.56%) were the principal constituents in the essential oil from Mentha pulegium L. These essential oils (in concentrations ranging from 3.91 to 500 μL·mL-1) showed satisfactory activities against Escherichia coli, Listeria monocytogenes, Salmonella choleraesuis and Staphylococcus aureus. The antioxidant activities with 2-deoxyribose and phosphomolybdenum and the reducing power (in concentrations ranging from 0.78 to 1000 μL·mL-1) were determined. The antioxidant activity was observed for the two oils evaluated by the phosphomolybdenum and 2-deoxyribose methods, whereas the essential oil from M. viridis presented low antioxidant activity in the reducing power assay.

关键词： Mentha Linalool Pulegone Volatiles

Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

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American Journal of Plant Sciences 2015年第16期6卷 2664-2671页

作者： Christiane Maria de Oliveira Maria das Gracas Cardoso Marisa Ionta Marisi Gomes Soares Juliana de Andrade Santiago Guilherme álvaro Ferreira da Silva Maria Luisa Teixeira danúbia ap.recida de carvalho selvati rezende Rafaela Vieira de Souza Luana Isac Soares David Lee Nelson Marcos Schleiden Sousa carvalho department of Chemistry Federal University of LavrasLavrasBrazil Institute of Biomedical Sciences Federal University of AlfenasAlfenasBrazil department of Chemistry Federal University of AlfenasAlfenasBrazil Federal University of Vales de Jequitinhonha e Mucuri DiamantinaBrazil department of Agriculture Federal University of LavrasLavrasBrazil

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

关键词： Cancer Volatile Oil Escova-de-Garrafa(Bottle Brush)

Essential Oils from the Leaves and Flowers of Callistemon viminalis:Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities

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American Journal of Plant Sciences 2017年第10期8卷 2516-2529页

作者： Thais ap.recida Sales Maria Das Gracas Cardoso Luiz Gustavo de Lima Guimaraes Karen Caroline Camargo danúbia ap.recida de carvalho selvati rezende Rafaela Magalhães Brandao Rafaela Vieira Souza Vanúzia Rodrigues Fernandes Ferreira Ana Ermelinda Marques Maísa Lamounier Magalhaes David Lee Nelson epartment of Chemistry Federal University of LavrasLavrasBrazil department of Natural Science Federal University of Sao Joao Del ReiSao Joao Del ReiBrazil department of Food Science Federal University of LavrasLavrasBrazil Pro-Reitoria de Pesquisa e Pós-Graduacao Federal University of the Jequitinhonha and Mucuri ValleysDiamantinaBrazil

The chemical compositions of the essential oils from the leaves and flowers of Callistemon viminalis and their insecticide and fungitoxic activities were determined. The essential oil was extracted by the hydrodistillation method using a modified Clevenger apparatus. The chemical characterization was performed by GC-MS and GC-FID. The evaluation of the insecticidal activity was performed with the Myzus persicae aphid, and the antifungal potential was determined via the inhibition of the mycelial growth of Alternaria alternata, Fusarium oxysorum and Botrytis cinérea phytopathogenic fungi. The principal components of the essential oils from the leaves and flowers were eucalyptol (84.60% and 61.47%), α-pinene (10.28% and 21.48%) and α-terpineol (2.59% and 2.79%), respectively. The use of a 0.5% concentration of the essential oil from the flowers influenced the preference of aphids and their reproduction. The number of adult aphids decreased within a period of 48 hours in the presence of the essential oil from the leaves. In the test with no chance of choice, the mean number of adults decreased with both oils within 48 hours. No inhibition of the mycelial growth of the A. alternate microorganism was observed in any of the treatments with the two essential oils. However, the growth of F. oxysporum and B. cinerea fungi was inhibited. The mycelial growth of F. oxysporum was inhibited with the concentration of 100 μL·Lˉ1 of the oil from the flowers and 250 μL·Lˉ1 of the oil from the leaves. The inhibition of the growth of B. cinerea was observed at concentrations of 500 and 100 μL·Lˉ1 for the oils from the flowers and leaves, respectively. The essential oils presented insecticidal and antifungal potentials. However, further studies are needed for these oils to be used in integrated pest management.

关键词： Myzus persicae Biological Activity Phytopathogenic Fungi Natural Products

Characterization of the Biological Potential of the Essential Oils from Five Species of Medicinal Plants

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American Journal of Plant Sciences 2017年第2期8卷 154-170页

作者： danubia ap.recida carvalho selvati rezende Rafaela Vieira Souza Maisa Lamounier Magalh?es Alex Rodrigues Silva Caetano Marcos Schleiden Sousa carvalho Ellen Cristina de Souza Luiz Gustavo de Lima Guimaraes David Lee Nelson Luís Roberto Batista Maria das Gra?as Cardoso departamento de Química Universidade Federal de LavrasLavrasBrasil departamento de Ciencia dos Alimentos Universidade Federal de LavrasLavrasMGBrasil departamento de Ciência dos Alimentos Universidade Federal de LavrasLavrasMGBrasil departamento de Agricultura Universidade Federal de LavrasLavrasMGBrasil Universidade Federal de Sao Joao del-Rei Sao Joao del-ReiBrasil Universidade Federal do Vale do Jequitinhonha e Mucuri DiamantinaMGBrasil

Essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation, chemically characterized and quantified by GC/MS and GC/FID. The principal componentes in the essential oil of M. piperita (carvone and limonene), C. citratus (geranial, neral and myrcene), R. officinalis (1,8-cineole, camphor and α-pinene), P. boldus (α-terpinyl formate, p-cymene and 1,8-cineole) and F. vulgare (methyl chavicol, limonene and fenchone) were identified. The oils were tested for antioxidant activity employing the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical-capture method, the oxidation of the β-carotene/linoleic acid system, and the ABTS radical-capture method. The essential oils were not effective. The antioxidant activities of the oils were determined by the hydroxyl method, and the highest activity (62.80%) was observed with the essential oil from M. piperita. Activities of 1.54 and 1.82 μg μg-1, respectively, were observed for the essential oils from C. citratus and P. Boldus using the phosphomolybdenum method. No activity was observed with respect to reducing power. The essential oil from C. citratus was effective in inhibiting the growth of L. monocytogenes (15.63 mg·L-1), S. choleraesuis (15.63 mg·L-1) and E. coli (31.25 mg·L-1), and the essential oil from P. boldus inhibited the growth of S. aureus (62.5 mg·L-1). No essential oil inhibited the growth of P. aeruginosa. A low antioxidant activity and a promising antibacterial activity were observed for these essential oils.

关键词： Natural Products Antioxidant Antibacterial Activity

Influence of Cold Storage on the Bioactivity Properties and the Quality of the Juice of Moro Blood Orange(Citrus sinensis(L.)Osbeck)

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American Journal of Plant Sciences 2019年第1期10卷 24-37页

作者： Maisa Lamounier Magalhaes Luiz Carlos de Oliveira Lima Allan da Silva Lunguinho danubia ap.recida de carvalho selvati rezende Vanuzia Rodrigues Fernandes Ferreira Rafaela Magalhaes Brandao Josefina ap.recida de Souza Ellen Cristina de Souza Kátia Júlia de Almeida David Lee Nelson Maria Das Gracas Cardoso departamento de Ciencia dos Alimentos Universidade Federal de Lavras(UFLA)LavrasBrazil departamento de Química Universidade Federal de Lavras(UFLA)LavrasBrazil Programa de Pos-Graduacao em Biocombustívis Universidade Federal do Vale do Jequitinhonha e MucuriDiamantinaBrazil

The possibility of commercialization of Moro blood oranges in tropical countries such as Brazil was evaluated to verify whether post-harvest management through storage at low temperatures for a period of 60 days can improve the bioactive properties and quality parameters. Moro blood oranges cultivated in Brazil did not contain significant amounts of anthocyanins at the time of harvesting, but these compounds were activated by post-harvest management through storage at low temperatures (4°C and 8°C) for a period of 60 days. The emergence of the anthocyanins in the juices occurred within a few weeks of storage, but the maximum levels were attained after 60 days and at the temperature of 8°C. Cold storage positively influenced other bioactive compounds such as total phenolic compounds, individual phenolic compounds, β-carotene and the antioxidant activity determined by the sequestration of DPPH free radicals. It did not influence the vitamin C content. In addition, storage significantly altered the color, total acidity and pH of the fruits, but it did not prevent its commercial use. The remaining quality parameters were not influenced. It is possible to commercialize these oranges in Brazil through post-harvest management.

关键词： Cyanidin-3-Glycoside Antioxidant Post-Harvest

Essential Oils from Mentha piperita,Cymbopogon citratus,Rosmarinus officinalis,Peumus boldus and Foeniculum vulgare:Inhibition of Phospholipase A_(2) and Cytotoxicity to Human Erythrocytes

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American Journal of Plant Sciences 2017年第9期8卷 2196-2207页

作者： danubia ap.recida de carvalho selvati rezende Maria das Gracas Cardoso Rafaela Vieira Souza Maria Luisa Teixeira Rafaela Magalhaes Brandao Vanuzia Rodrigues Fernandes Ferreira Jéssica Oliveira e Nogueira Maisa Lamounier Magalhaes Silvana Marcussi David Lee Nelson departamento de Química Universidade Federal de Lavras Lavras Brasil departamento de Ciência dos Alimentos Universidade Federal de Lavras Lavras Brasil Universidade Federal do Vale do Jequitinhonha e Mucuri Diamantina Brasil

The essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation and characterized and quantified by GC-MS and GC-DIC. The oils induced hemolysis with all the doses evaluated (0.6 to 1.8 μL), and the diameters of the halos varied between 9 and 15 mm. Pre-incubation of P. boldus oil with Bothrops jararacussu venom resulted in potentiation of venom-induced hemolysis (30%) (proteases and phospholipases A2). The essential oil from M. piperita (0.6 μL) inhibited venom-induced hemolysis by 45%, whereas 0.6 μL of R. officinalis oil increased the hemolysis by 20%. For the essential oil from F. vulgare, 100% inhibition of activity (0.6 and 1.2 μL) was observed. The application of C. citratus oil induced hemolysis with all the volumes evaluated. Phospholipase activity induced by the venom was only inhibited (10%) with the 0.6 μL volume of R. officinalis oil. The oils from M. piperita and F. vulgare (1.8 μL) and C. citratus oil (0.6 μL) potentiated the phospholipase activity. The results highlight the need for a broad characterization and regulation of the use of natural products, because they can have therapeutic or toxic actions.

关键词： Natural Products Snake Venoms Hemolysis Pharmacological Potential

Normative data for TM electrocochleography measures

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Journal of Otology 2017年第2期12卷 68-73页

作者： Signe Schuster Grasel Roberto Miquelino de Oliveira Beck Ricardo Silva Chiabai Loureiro Amanda Costa Rossi Edigar rezende de Almeida John Ferraro University of Sao Paulo School of Medicine Dr.Eneas de Carvalho Aguiar255sala 6173Cerqueira CesarSao PauloSP CEP 05403-000Brazil University of Kansas Medical Center Kansas CityKS 66160USA

Objective: Establish normative data for tympanic electrocochleography(TM ECoch G) parameters in normal hearing adults without Meniere's disease's(MD) symptoms. describe TM ECoch G variables that help to distinguish normal from MD *** and methods: We enrolled 100 subjects(N =200 ears), 59 females, aged between 19 and 71 years from 09/2010 to 04/2014. Inclusion criteria: normal otomicroscopy, hearing thresholds≤25 dB nHL from 250 to 4000 Hz, normal tympanogram, no symptoms of MD according to the AAO-HNS 1995 criteria and Gibson's score ded subjects with dizziness, aural fullness or other symptoms of endolymphatic hydrops. The following parameters were analyzed: SP/ap amplitude ratio, SP/ap area ratio and the difference between ap latency with rarefaction and condensation ***: There was no significant difference between right and left ears(Intraclass correlation coefficient ap amplitude ratio varied between 0.084 and 0.356 and SP/ap area ratio between 0.837 and 1.671(percentiles 5 and 95). The ap latency difference to rarefaction and condensation clicks was between 0.0 and 0.333 ***: Normative data for TM ECoch G parameters were established in 100 normal hearing subjects without MD. These data can be used to distinguish normal from pathological findings and in follow-up of MD patients.

关键词： Electrocochleography Meniere disease Hearing Dizziness Tinnitus