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The Use of the PCR-Based Dot-Blot Hybridization Assay to Detect Resistance Markers to Rifampicin and Streptomycin in Mycobacterium tuberculosis Isolates from the SW Region of Cameroon

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Journal of Tuberculosis Research 2016年第2期4卷 72-79页

作者： Irene Ane-Anyangwe Wilfred Fon Mbacham Henry Dilonga Meriki Teyim Pride Theresa Nkuo-Akenji Veronique Mbeng Penlap Leopold Djomkam Tietcheu damian nota anong Akindeh Mbuh Nji Vincent P. K. Titanji Department of Biochemistry and Microbiology Faculty of Science University of Buea Buea Cameroon Laboratory for Public Health Research Biotechnologies Biotechnology Centre University of Yaoundé Yaoundé Cameroon Mezam Polyclinic Bamenda Cameroon

Drug sensitivity testing to establish resistance to TB drugs takes many months to arrive at. Public health physicians have difficulties with such an approach due to long wait periods and cannot use it to establish community wide prevalence as a way to understand where resistance may be emerging faster and to limit its spread. The objective of this study was to use the dot-blot hybridization technique in the detection of resistance to rifamycin (RIF) and streptomycin (SM) in South- Western Cameroon and to compare the technique with the routine culture and drug susceptibility testing for detecting resistance in a resource poor country, Cameroon. A hospital-based study was conducted at the Regional hospitals of Buea and Limbe and Tiko Central Clinic. Tuberculosis (TB) patients aged 15 to 50 (mean age: 30.50 ± 8.33 standard deviation) were recruited for the study between December 2006 and April 2007. Cultures from 59 patients were tested for rifampicin and streptomycin sensitivity by the modified proportion method and mutational analysis for rpoB codon 516 and rrs codon 513 was performed by the dot-blot hybridization technique. Of the 59 sputum samples collected (36 were males and 23 were females) came from Buea 19 (32.2%), Limbe 20 (33.9%) and Tiko 20 (33.9%) towns respectively. Amplification for the gene showed that there was (59) 100% amplification with primers used for rpoB genes and 43 (72.9%) amplification with primers used for the rrs gene. Mutational analysis demonstrated that resistance to RIF was common in females (52.1%) than males (41.7%) while 6% of the samples were indeterminate. 12 (20.3%) samples showed phenotypic and genotypic resistance to RIF compared to 34 samples (58.1%) for SM. Phenotypic resistance and genotypic susceptibility were found in 5 (8.5%) RIF and 3 (4.7%) SM compared to phenotypic susceptibility and genotypic resistance that were found in 2 (3.5%) RIF and 3(4.7%) SM. Double mutation on rpoB and rrs genes occurred in 8 (13.6%) DNA sample

关键词： PCR-Based DOT-Blot Analysis Rifamycin Streptomycin SW Region

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A Review on Brucellosis in Cameroon: Diagnostic Approaches, Epidemiology and Risk Factors for Infection

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Advances in Microbiology 2022年第7期12卷 415-442页

作者： Seraphine Mojoko Eko Seraphine Nkie Esemu anong damian nota Lucy Mande Ndip Department of Biomedical Sciences Faculty of Health Sciences University of Buea Buea Cameroon Department of Microbiology and Parasitology Faculty of Science University of Buea Buea Cameroon Laboratory for Emerging Infectious Diseases University of Buea Buea Cameroon Department of Biological Sciences Faculty of Science University of Bamenda Bamenda Cameroon

Brucellosis is a neglected tropical zoonotic disease that threatens the food production and public health sectors. It is of considerable animal welfare and economic importance and is underreported in most parts of the world, especially in developing countries like Cameroon. Brucellosis has been reported in cattle, other domestic animals and humans in Cameroon. The burden of the disease is unclear, and the awareness remains questionable. It became necessary for this review to be carried out to highlight the diagnostic approaches used to confirm brucellosis in animals and humans, disease epidemiology and risk factors for infection. So far, reports of brucellosis in previous studies have been based on serology only. Seroprevalence data of Brucella antibodies in animals indicate the risk of human brucellosis in Cameroon. However, few investigations have been undertaken on human brucellosis, considering the different epidemiological settings. There is no report or unsuccessful attempts to identify Brucella species circulating in Cameroon. It could largely be attributed to a lack of standard laboratories for testing and the lack of consumables. The way forward will require a surveillance system for brucellosis in the country, educating all sectors affected and drafting a diagnostic protocol for high-risk individuals.

关键词： Brucella Brucellosis Epidemiology Diagnosis Risk Factors Cameroon

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Immuno-haematologic and virologic responses and predictors of virologic failure in HIV-1 infected adults on first-line antiretroviral therapy in Cameroon

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Infectious Diseases of Poverty 2014年第1期3卷 29-39页

作者： Henry D Meriki Kukwah A Tufon Mbunkah H Afegenwi Bernard A Nyindem Pascal N Atanga damian N anong Fidelis Cho-Ngwa Theresa Nkuo-Akenji Department of Microbiology and Parasitology University of BueaP.O.Box 63BueaCameroon BioCollections Worldwide Inc.Regional Office BueaCameroon Regional Delegation of Public Health Southwest RegionCameroon Faculty of Science Clinical Diagnostic Laboratory University of BueaBueaCameroon

Background:Contemporary data on the immunologic,haematologic and virologic responses and predictors of virologic failure after initiation of free antiretroviral treatment in Cameroon are needed to evaluate the current treatment-monitoring algorithm and to complement efforts to scale-up and improve on the management of HIV ***:This was a cross-sectional study conducted between October 2010 and June 2012.A total of 951 participants aged 18-74 years were recruited from selected approved HIV treatment centres of the Northwest and Southwest *** comprised 247 males and 704 ***,self-reported risk behaviours and socioeconomic data were obtained using a structured *** blood and CD4+T-cell counts were done using standard automated *** of viral load(VL)was done using Abbott RealTime HIV-1 m2000™*** was analysed using SPSS version *** statistical significance level was P500 cells/μL *** was present in 26.2%of the participants with 62.3%,25.7%and 12%described as mild,moderate and severe anaemia *** failure occurred in 23.2%of the participants with 12.3%having VL>10,000 RNA copies/*** 76.8%of patients attained adequate viral suppression with 40.8%having undetectable viral *** age group 18-29 years(p=0.024),co-infection with tuberculosis(p=0.014),anaemia(p=0.028)and distance from the treatment centre(p=0.011)independently predicted virologic ***:The majority of the participants achieved adequate viral suppression after≥6 months of *** these favourable immuno-haematologic and virologic outcomes,the National AIDS Control Program should step-up efforts to improve on antir

关键词： Immuno-haematologic Predictors Virologic failure Antiretroviral therapy

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