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An ethically guided preclinical device for phenotyping H_(2)production in laboratory rodents

Animal Models and Experimental Medicine 2024年第4期7卷 553-561页

作者： Victor Pascal-Moussellard Emilie Boucher Stéphane Tanguy Philippe Cinquin Pierre-Alain Barraud ChloéDavin Cordélia Salomez-Ihl dalil hannani François Boucher Jean-Pierre Alcaraz University Grenoble Alpes CNRSUMR 5525VetAgro SupGrenoble INPTIMCGrenobleFrance

Background:Dihydrogen(H_(2))is produced endogenously by the intestinal microbiota through the fermentation of diet *** the past few years,numer-ous studies have demonstrated the significant therapeutic potential of H_(2)in various pathophysiological contexts,making the characterization of its production in labora-tory species of major preclinical ***:This study proposes an innovative solution to accurately monitor H_(2)pro-duction in free-moving rodents while respecting animal welfare *** devel-oped device consisted of a wire rodent cage placed inside an airtight chamber in which the air quality was maintained,and the H_(2)concentration was continuously *** the airtightness and efficiency of the systems used to control and maintain air quality in the chamber were checked,tests were carried out on rats and mice with different metabolic phenotypes,over 12 min to 1-h experiments and repeatedly.H_(2)production rates(HPR)were obtained using an easy calculation algorithm based on a first-order moving ***:HPR in hyperphagic Zucker rats was found to be twice as high as in control Wistar rats,respectively,2.64 and 1.27 nmol.s^(−1)per *** addition,the ingestion of inulin,a dietary fiber,stimulated H_(2)production in *** were 0.46 nmol.s^(−1)for animals under control diet and 1.99 nmol.s^(−1)for animals under inulin ***:The proposed device coupled with our algorithm enables fine analysis of the metabolic phenotype of laboratory rats or mice with regard to their endogenous H_(2)production.

关键词： hydrogen therapy laboratory rodents microbiota molecular hydrogen noninvasive monitoring device

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