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腹腔镜结直肠癌根治患者手术前后外周血免疫细胞表达变化及其与短期预后的关系

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检验医学与临床 2024年第10期21卷 1455-1460,1467页

作者：余强张大洪吕权向征重庆医科大学附属第一医院胃肠外科重庆400016

目的探讨结直肠癌患者腹腔镜根治性结直肠癌切除手术前后外周血中免疫细胞的变化,并进一步分析其与短期预后的关系。方法选择2021年3月至2022年3月该院收治的418例结直肠癌患者纳入结直肠癌组,136例门诊体检的健康志愿者纳入对照组,所... 详细信息

目的探讨结直肠癌患者腹腔镜根治性结直肠癌切除手术前后外周血中免疫细胞的变化,并进一步分析其与短期预后的关系。方法选择2021年3月至2022年3月该院收治的418例结直肠癌患者纳入结直肠癌组,136例门诊体检的健康志愿者纳入对照组,所有结直肠癌患者均接受腹腔镜根治性结直肠癌切除手术,术前和术后1、3、5 d采集患者外周静脉血,采用流式细胞仪检测外周血中Cd3^(+)T淋巴细胞、Cd4^(+)T淋巴细胞、Cd8^(+)T淋巴细胞、Cd4^(+)/Cd8^(+)双阳性T淋巴细胞、Cd19^(+)B淋巴细胞,并计算Cd4^(+)/Cd8^(+)比值。术后随访至2023年3月31日,收集患者术后复发、转移和生存情况,将复发、转移定义为预后不良,无复发、转移定义为预后良好,分析预后不良组与预后良好组外周血免疫细胞的差异。结果结直肠癌组术前外周血Cd3^(+)T淋巴细胞、Cd4^(+)T淋巴细胞、Cd4^(+)/Cd8^(+)比值明显低于对照组,Cd8^(+)T淋巴细胞、Cd4^(+)Cd8^(+)双阳性T淋巴细胞、Cd19^(+)B淋巴细胞明显高于对照组,差异有统计学意义(Pd3^(+)T淋巴细胞、Cd4^(+)T淋巴细胞、Cd4^(+)/Cd8^(+)比值先降低再升高,Cd8^(+)T淋巴细胞、Cd4^(+)Cd8^(+)双阳性T淋巴细胞、Cd19^(+)B淋巴细胞先升高后降低,且术后5 d外周血Cd3^(+)T淋巴细胞、Cd4^(+)T淋巴细胞、Cd4^(+)/Cd8^(+)比值高于术后1 d,差异有统计学意义(Pd Cd8^(+)T淋巴细胞、Cd4^(+)Cd8^(+)双阳性T淋巴细胞、Cd19^(+)B淋巴细胞低于术后1 d,差异有统计学意义(Pd Cd4^(+)/Cd8^(+)比值低于预后良好组,Cd4^(+)Cd8^(+)双阳性T淋巴细胞、Cd19^(+)B淋巴细胞高于预后良好组,差异有统计学意义(Pd低Cd4^(+)/Cd8^(+)比值、高Cd4^(+)Cd8^(+)双阳性T淋巴细胞、高Cd19^(+)B淋巴细胞结直肠癌患者无疾病进展生存率低于高Cd4^(+)/Cd8^(+)比值、低Cd4^(+)Cd8^(+)双阳性T淋巴细胞、低Cd19^(+)B淋巴细胞结直肠癌患者(Pm分期Ⅲ期、淋巴结转移、术后放疗、术后5 d Cd4^(+)Cd8^(+)双阳性T淋巴细胞升高、术后5 d Cd19^(+)B淋巴细胞升高是结直肠癌患者预后不良的危险因素(Pd Cd4^(+)/Cd8^(+)比值升高是结直肠癌患者预后不良保护因素(Pd4^(+)/Cd8^(+)比值、高Cd4^(+)Cd8^(+)双阳性T淋巴细胞、高Cd19^(+)B淋巴细胞与术后短期内不良预后有关,可作为结直肠癌术后预后评估的指标。

关键词：结直肠癌免疫细胞短期预后 T淋巴细胞 B淋巴细胞

The Nonlinear Wave Force on a Circular Cylinder in Shallow Water

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China Ocean Engineering 1992年第2期7卷 117-136页

作者： Qiu dahong

Based on the 1st order cnoidal wave theory, the nonlinear wave diffraction around a circular cylinder in shallow water is studied in this paper. The equation of the wave surface around the cylinder is formulated and by using this formula the wave surface elevation on the cylinder surface can be obtained. In this paper, the formula for calculating the cnoidal wave force on a circular cylinder is also derived. For the wave conditions which are often encountered in practical engineering designs, the ratios of the nonlinear wave forces to the linear wave forces are calculated, and the results are plotted in this paper for design purposes. In order to verify the theoretical results, m.d.l tests are conducted. After comparing the test results with the theoretical ones, it is concluded that, in shallow water, for the case of T g / d^(1/2) > 8-10 and H / d > 0.3, the cnoidal wave theory should be used to calculate the wave action on a cylindrical pier.

关键词： cnoidal wave force cylinder shallow water

不同铵态氮肥配施对麦茬水稻生长、氮素利用及产量的影响

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长江大学学报（自然科学版） 2024年第3期21卷 114-122页

作者：卢碧林张大弘罗嘉润杨松长江大学农学院湖北荆州434025 湿地生态与农业利用教育部工程研究中心(长江大学) 湖北荆州434025 涝渍灾害与湿地农业湖北省重点实验室(长江大学) 湖北荆州434025 湖北省荆州市荆州区东城街道办事处综合执法中心湖北荆州434100

碳铵施用可促进稻田秸秆腐解和秧苗生长,但碳铵易分解和不能造粒使其应用受限,研究碳铵替代铵态氮肥将促进该技术的生产应用。基于2年小区定位试验和统计分析,以不施肥(CK)为对照,设置总施氮量20%碳铵+30%复合肥(V1)、20%氯化铵+30%复合... 详细信息

碳铵施用可促进稻田秸秆腐解和秧苗生长,但碳铵易分解和不能造粒使其应用受限,研究碳铵替代铵态氮肥将促进该技术的生产应用。基于2年小区定位试验和统计分析,以不施肥(CK)为对照,设置总施氮量20%碳铵+30%复合肥(V1)、20%氯化铵+30%复合肥(V2)、20%磷酸二铵+30%复合肥(V3)、20%硫酸铵+30%复合肥(V4)等5个不同的速效氮肥+复合肥的铵态氮肥筛选试验,研究不同铵态氮肥对麦茬水稻生长、氮素利用及产量的影响。结果表明:处理V2产量达10.34×10^(3)kg/hm^(2),较其他处理均达到显著增产的效果,处理V1、V3、V4间产量差异不显著;处理V2的平均穗粒数、叶面积指数和干物质积累量显著高于其他处理;施肥后12 d,处理V2的铵态氮含量显著性大于其他处理,硝态氮含量显著大于除处理V4外的其他处理,处理V2的pH低于其他处理,达显著水平;处理V2较其他处理显著提升茎蘖数7.48%~20.9%,处理V2与其他速效铵态氮肥处理相比显著地提升了氮素积累量、氮素表观利用率、氮素农学利用率和偏生产力。可见氯化铵配施可降低土壤pH,增加土壤铵态氮和硝态氮的含量,增加水稻平均穗粒数,提高氮素利用效率,具有平衡秸秆腐解和作物产量关系的作用。

关键词：速效氮肥铵态氮水稻生长土壤理化性质氮素利用率

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Glacier changes in the Sanjiangyuan Nature Reserve of China during 2000-2018

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Journal of Geographical Sciences 2022年第2期32卷 259-279页

作者： ZHANG Yuan YAO Xiaojun ZHOU Sugang ZHANG dahong College of Geography and Environment Sciences Northwest Normal UniversityLanzhou 730070China College of Urban and Environmental Sciences Northwest UniversityXi’an 710027China

The glaciers in the Sanjiangyuan Nature Reserve of China(SNRC)are a significant water resource for the Yangtze,Yellow,and mekong *** on Landsat Thematic mapper(Tm)/Operational Land Imager(OLI)images acquired in 2000,2010,and 2018,the outlines of glaciers in the SNRC were obtained by combining band ratio method with manual *** were 1714 glaciers in the SNRC in 2018,with an area of 2331.15±54.84 km^(2),an ice volume of 188.90±6.41 km3,and an average length of 1475.4±15 *** 2000-2018,the corresponding values of glaciers decreased by 69,271.95±132.06 km^(2),18.59±8.83 km3,and 84.75±34 m,*** in the Yangtze River source area witnessed the largest area loss(-154.45 km^(2)),whereas glaciers in the mekong River source area experienced the fastest area loss(-2.02%·a-1)and the maximum reduction of the average length(-125.82 m).Overall,the retreat of glaciers in the SNRC exhibited an accelerating ***,the loss rate of glacier area in the Yellow River source area in 2010-2018 was more than twice that in *** glacier change is primarily attributed to the significant rise in temperature during the ablation *** other factors including the size,orientation and terminus elevation of glaciers also contributed to the heterogeneity of glacier change.

关键词： glacier climate change nature reserve Yangtze River Yellow River mekong River

A practical strategy to develop isoform-selective near-infrared fluorescent probes for human cytochrome P450 enzymes

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Acta Pharmaceutica Sinica B 2022年第4期12卷 1976-1986页

作者： Lei Feng Xiangge Tian dahong Yao Zhenlong Yu Xiaokui Huo Zhenhao Tian Jing Ning Jingnan Cui Tony d.James Xiaochi ma Second Affiliated Hospital Dalian Medical UniversityDalian 116023China College of Pharmacy the National&Local Joint Engineering Research Center for Drug Development of Neurodegenerative DiseaseDalian Medical UniversityDalian 116044China School of Pharmaceutical Sciences Shenzhen Technology UniversityShenzhen 518060China State Key Laboratory of Fine Chemicals Dalian University of TechnologyDalian 116024China department of Chemistry University of BathBath BA27AYUK School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

Currently,the development of selective fluorescent probes toward targeted enzymes is still a great challenge,due to the existence of numerous isoenzymes that share similar catalytic ***,a double-filtering strategy was established to effectively develop isoenzyme-specific fluorescent probe(s)for cytochrome P450(CYP)which are key enzymes involving in metabolism of endogenous substances and *** the first-stage of our filtering approach,near-infrared(NIR)fluorophores with alkoxyl group were prepared for the screening of CYP-activated fluorescent substrates using a CYPs-dependent incubation *** the second stage of our filtering approach,these candidates were further screened using reverse protein-ligand docking to effectively determine CYP isoenzyme-specific probe(s).Using our double-filtering approach,probes S9 and S10 were successfully developed for the real-time and selective detection of CYP2C9 and CYP2J2,respectively,to facilitate high-throughput screening and assessment of CYP2C9-m.d.ated clinical drug interaction risks and CYP2J2-associated disease *** observations suggest that our strategy could be used to develop the isoform-specific probes for CYPs.

关键词： Enzyme activity bioimaging Cytochrome P450 drugedrug interactions Biomarker analysis Fluorescent probe

Novel Graphene Biosensor Based on the Functionalization of multifunctional Nano-bovine Serum Albumin for the Highly Sensitive detection of Cancer Biomarkers

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Nano-micro Letters 2019年第2期11卷 24-36页

作者： Lin Zhou Kun Wang Hao Sun Simin Zhao Xianfeng Chen dahong Qian Hongju mao Jianlong Zhao State Key Laboratory of Transducer Technology Key Laboratory of Terahertz Solid-State TechnologyShanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050People’s Republic of China School of Biom.d.cal Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Electronic Engineering Bangor UniversityBangor LL57 1UTUK

A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer *** novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate *** form.d.nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EdC and *** nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic *** improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg *** electrical detection of the binding of CEA followed the Hill m.d.l for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×*** multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2d nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanom.d.cine,and drug delivery.

关键词： Bio-interface multifunctional denatured BSA GFET biosensor Cancer biomarker

吉非替尼不同给药时间对肺癌模型小鼠不良反应及表皮生长因子受体、基质金属蛋白酶-9表达的影响

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中华实验外科杂志 2024年第4期41卷 764-766页

作者：尹波赵永强朱应超张磊李大宏鲁铭山东第一医科大学附属人民医院胸心外科济南271199 山东大学齐鲁医院胸外科济南250063

目的探讨吉非替尼不同时间用药对肺癌模型小鼠不良反应及表皮生长因子受体(EGFR)和基质金属蛋白酶-9(mmP-9)表达的影响。方法70只肺癌模型小鼠简单随机抽样法分为对照组、4 h组、8 h组、12 h组、16 h组、20 h组、24 h组,对照组给予等量... 详细信息

目的探讨吉非替尼不同时间用药对肺癌模型小鼠不良反应及表皮生长因子受体(EGFR)和基质金属蛋白酶-9(mmP-9)表达的影响。方法70只肺癌模型小鼠简单随机抽样法分为对照组、4 h组、8 h组、12 h组、16 h组、20 h组、24 h组,对照组给予等量含羧甲基纤维素钠的蒸馏水,其余均给予50 mg/kg吉非替尼悬浮液灌喂,连续给药21 d。比较各组抑瘤效果、血清白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量、肿瘤组织EGFR与mmP-9基因相对表达量。采用单因素方差进行统计学分析。结果8 h组抑瘤效果[(59.42±7.54)%]高于4 h组[(50.54±7.12)%]、12 h组[(44.20±6.45)%]、16 h组[(35.87±5.36)%]、20 h组[(31.52±5.24)%]、24 h组[(40.94±5.32)%,PmmP-9基因相对表达量[(1.32±0.56)、(1.23±0.02)ng/L]高于4 h组[(0.48±0.09)、(0.54±0.04)ng/L]、8 h组[(0.29±0.04)、(0.32±0.07)ng/L]、12 h组[(0.56±0.07)、(0.78±0.12)ng/L]、16 h组[(0.78±0.10)、(0.83±0.05)ng/L]、20 h组[(0.89±0.08)、(0.86±0.08)ng/L]、24 h组[(0.96±0.12)、(0.98±0.06)ng/L,F=105.45、92.72,P<0.05]。结论吉非替尼4、8 h给药抑瘤效果最好,不良反应较轻。

关键词：肺癌吉非替尼表皮生长因子基质金属蛋白酶-9 时辰节律性

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一种水平线无板停机的节能改造

印制电路信息 2024年第11期32卷 59-61页

作者：赵汝垣周明吉刘世裕胡大红陈勇坚珠海方正科技高密电子有限公司广东珠海510310

在行业内部竞争中,厂内运行成本是关键。针对湿法水平线的智能程序进行研究,在不增加硬件的情况下,通过修改可编程控制器(PLC)程序,在该设备运行过程中,减少加压泵浦和传送电机的运行时间,从而大幅降低该设备的水电成本。节能效果为25%~... 详细信息

在行业内部竞争中,厂内运行成本是关键。针对湿法水平线的智能程序进行研究,在不增加硬件的情况下,通过修改可编程控制器(PLC)程序,在该设备运行过程中,减少加压泵浦和传送电机的运行时间,从而大幅降低该设备的水电成本。节能效果为25%~40%,达到节能减排、降本增效的目的,特别适合小批量快单、研发单、设备稼动率不足、长期待机等首件的工厂。此改造不仅为工厂降成本持续发力,同时也是积极响应国家推行的节能政策。

关键词：智能程序水电成本节能效果小批量快单

Effect of esculetin on bone metabolism in ovariectomized rats

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Journal of Traditional Chinese m.d.cine 2018年第6期38卷 896-903页

作者： Liu meijie Wang Ruihai Li Yan Bai dong Pan Jinghua Liu Hong Wang Shaojun Wu Jiaying Sun Gang miao Qing Ju dahong Liu Limei Bone and Joint diseases Laboratory Experimental Research CenterChina Academy of Chinese Medicine Science 100700China The Basic Research Center of Recipe and Syndrome in Traditional Chinese m.d.cine Institute of Basic Theory for Chinese MedicineChina Academy of Chinese Medicine ScienceBeijing 100700China

OBJECTIVE: To determine the effect of an esculetin formulation(at 97.4% purity) on osteoporosis, and to investigate the potential underlying molecular mechanism(s).mETHOdS: Sixty specific pathogen free-grade female Wistar rats were randomly assigned to three groups: blank control(n = 12), sham(n = 12), and m.d.l(n = 36). The m.d.l group were bilaterally ovariectomized. The sham group had the tissue surrounding the ovaries removed, while the ovaries were retained. After 3 months, the m.d.l group was randomly divided into three subgroups: OVX(n = 12), positive control(n = 12), and esculetin(n =12). The positive control group and the esculetin group were intragastrically administered diethylstilbestrol(0.046 mgkd^(-1)), respec·kg^(-1)tively,·d^(-1)) or esculetin(384 mgg^(-1) once per day for 6 consecu··-tive days; m.d.cation administration was then stopped for 1 d, before being administered for another 6 consecutive days. All rats were treated for 3months. Samples were collected at the end of the treatment period. An Osteocore3 digital 2d bone densitometer was used to test the bone mineral density, and histomorphometric analysis was perform.d.to measure bone mass, bone formation,and bone resorption. Enzyme-linked immunosorbent assay analysis was used to measure the serum concentrations of interleukin-6(IL-6), osteoprotegerin(OPG), and receptor activator of nuclear factor-kappa B ligand(RANKL). Immunohistochemistry and in situ hybridization were perform.d.to detect the protein and mRNA expressions of OPG and RANKL in osteoblasts and bone marrow stromal ***: Compared with the OVX group, the esculetin group had significantly greater femoral bone mineral density and tibial trabecular bone volume,and significantly smaller trabecular resorption surface. The percentage of trabecular formation surface, average osteoid width, trabecular bone mineralization rate, and cortical bone mineralization rate did not significantly differ between groups. Compared with the sham g

关键词： Esculetin Osteoporosis Osteoprotegerin RANK ligand Bone resorption