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Versatile effects of galectin-1 protein-containing lipid bilayer coating for cardiovascular applications

Bioactive Materials 2024年第12期42卷 207-225页

作者： Md Lemon Hasan Ju Ro Lee Khandoker Asiqur Rahaman dae hyeok yang Yoon Ki Joung Center for Biomaterials Biomedical Research InstituteKorea Institute of Science and Technology(KIST)Hwarangno 14-gil 5Seongbuk-guSeoul02792Republic of Korea Division of Bio-Medical Science&Technology University of Science and Technology(UST)113 GwahangnoYuseong-guDaejeon34113Republic of Korea Center for Systems Biology Massachusetts General Hospital Research InstituteBostonMA02114USA Department of Radiology Massachusetts General HospitalHarvard Medical SchoolBostonMA02114USA Institute of Cell and Tissue Engineering College of MedicineThe Catholic University of KoreaSeoul06591Republic of Korea KHU-KIST Department of Conversing Science and Technology Graduate SchoolKyung Hee UniversitySeoulRepublic of Korea

Modulating inflammatory cells in an implantation site leads to severe complications and still unsolved challenges for blood-contacting medical *** by the role of galectin-1(Gal-1)in selective functions on multiple cells and immunomodulatory processes,we prepared a biologically target-specific surface coated with the lipid bilayer containing Gal-1(Gal-1-SLB)and investigate the proof of the biological ***,lipoamido-dPEG-acid was deposited on a gold-coated substrate to form a self-assembled monolayer and then conjugated dioleoylphosphatidylethanolamine(DOPE)onto that to produce a lower leaflet of the supported lipid bilayer(SLB)before fusing membrane-derived vesicles extracted from B16-F10 *** Gal-1-SLB showed the expected anti-fouling activity by revealing the resistance to protein adsorption and bacterial *** vitro studies showed that the Gal-1-SLB can promote endothelial function and inhibit smooth muscle cell ***,Gal-1-SLB presents potential function for endothelial cell migration and angiogenic *** vitro macrophage culture studies showed that the Gal-1-SLB attenuated the LPS-induced inflammation and the production of macrophage-secreted inflammatory ***,the implanted Gal-1-SLB reduced the infiltration of immune cells at the tissue-implant interface and increased markers for M2 polarization and blood vessel formation in *** straightforward surface coating with Gal-1 can be a useful strategy for modulating the vascular and immune cells around a blood-contacting device.

关键词： Galectin-1 protein Supported lipid bilayer Macrophage Cardiovascular devices Inflammation

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Inhibiting scar formation via wearable multilayer stacked electret patch:Self-creation of persistent and customizable DC electric field for fibrogenic activity restriction

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InfoMat 2024年第3期6卷 66-80页

作者： Sung-Won Kim Sumin Cho Donghan Lee Jiyu Hyun Sunmin Jang Inwoo Seo Hyun Su Park Hee Jae Hwang Hyung-Seop Han dae hyeok yang Heung Jae Chun Suk Ho Bhang Dongwhi Choi School of Chemical Engineering Sungkyunkwan UniversitySuwonRepublic of Korea Department of Mechanical Engineering(Integrated Engineering Program) Kyung Hee UniversityYonginGyeonggiRepublic of Korea Biomaterials Research Center Biomedical Research DivisionKorea Institute of Science and Technology(KIST)SeoulRepublic of Korea Department of Medical Life Sciences College of MedicineInstitute of Cell and Tissue EngineeringThe Catholic University of KoreaSeoulRepublic of Korea

Electrical stimulation has recently received attention as noninvasive treatment in skin wound healing with its outstanding biological property for clinical ***,the complexity of equipment for applying appropriate electrical stimulation remains an ongoing ***,we proposed a strategy for skin scar inhibition by providing electrical stimulation via a multilayer stacked electret(MS-electret),which can generate direct current(DC)electric field(EF)without any power supply *** addition,the MS-electret can easily control the intensity of EFs by simply stacking electret layers and maintain stable EF with the surface potential of 3400 V over 5 days owing to the injected charges on the electret *** confirmed inhibition of type 1 collagen andα-SMA expression of human dermal fibroblasts(hDFs)by 90%and 44%in vitro,indicating that the transition of hDFs to myofibroblasts was restricted by applying stable electrical *** further revealed a 20%significant decrease in the ratio of myofibroblasts caused by the MS-electret in *** findings present that the MS-electret is an outstanding candidate for effective skin scar inhibition with a battery-free,physiological electrical microenvironment,and noninvasive treatment that allows it to prevent external infection.

关键词： DC electric field dermal fibroblasts electret scar formation restriction self-creation of electrical stimulation

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Effects of drought stress on root morphology and spatial distribution of soybean and adzuki bean

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Journal of Integrative Agriculture 2021年第10期20卷 2639-2651页

作者： Hyen Chung CHUN Sanghun LEE Young dae CHOI Dong hyeok GONG Ki Youl JUNG Crop Production Technology Research Division National Institute of Crop ScienceMiryang-si 50424Republic of Korea

Due to global climate change,Korea is facing severe droughts that affect the planting and early vegetative periods of upland *** and adzuki bean are important legume crops in Korea,so it is critical to understand their adaptations to water *** study investigated the changes in root morphological properties in soybean and adzuki bean and quantified the findings using fractal *** experiment was performed at the National Institute of Crop Science in Miryang,*** and adzuki beans were planted in test boxes and grown for 30 *** boxes were filled with bed soil with various soil moisture *** images were obtained and scanned every two days,and the root properties were characterized by root length,depth and surface area,number of roots,and fractal parameters(fractal dimension and lacunarity).Root depth,length and surface area and the number of roots increased in both crops as the soil moisture content *** fractal dimension and lacunarity values increased as the soil moisture content *** results indicated that the greater the soil moisture,the more heterogeneous the root *** analysis of the morphological properties and fractal parameters indicated that soybean and adzuki bean had different root structure *** soybean and adzuki bean were sensitive to the amount of soil moisture in the early vegetative *** required a soil moisture content greater than 70%of the field capacity to develop a full root structure,while adzuki bean required 100%of the field *** results would be useful in understanding the responses of soybean and adzuki bean to water stress and managing irrigation during cultivation.

关键词： soybean adzuki bean root morphology fractal dimension soil water content

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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-hyeok Kwon dae-Jin Jung dae-Hyun Kim Jun-Koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

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Perspective Projection Algorithm Enabling Mobile Device’s Indoor Positioning

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Journal of Computer and Communications 2018年第1期6卷 159-170页

作者： Seo Woo Han Yun Jung Lee Ji hyeok Yun Chang Yong Han dae Ho Lee Doug Young Suh Department of Electronics Engineering Kyung Hee University Yongin-si Republic of Korea

In order to improve the user’s satisfaction with the augmented reality (AR) technology and the accuracy of the service, it is important to obtain the exact position of the user. Frequently used techniques for finding outdoors locations is the global positioning system (GPS), which is less accurate indoors. Therefore, an indoor position is measured by comparing the reception level about access point (AP) signal of wireless fidelity (Wi-Fi) or using bluetooth low energy (BLE) tags. However, Wi-Fi and Bluetooth require additional hardware installation. In this paper, the proposed method of estimating the user’s position uses an indoor image and indoor coordinate map without additional hardware installation. The indoor image has several feature points extracted from fixed objects. By matching the feature points with the feature points of the user image, we can obtain the position of the user on the Indoor map by obtaining six or more pixel coordinates from the user image and solving the solution using the perspective projection formula. The experimental results show that the user position can be obtained more accurately in the indoor environment by using only the software without additional hardware installation.

关键词： Camera Pose Estimation Indoor Positioning Perspective Projection Homography

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HETEROEPITAXIAL GROWTH OF PB(MG1/3TA2/3)O3THIN FILMS ON SRTIO3 SUBSTRATES USING CHEMICAL SOLUTION DEPOSITION;MICROSTRUCTURAL EVOLUTION

HETEROEPITAXIAL GROWTH OF PB(MG1/3TA2/3)O3THIN FILMS ON SRTI...

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第8届国际材联电子材料国际研讨会

作者： dae-Soo Jeong Yeon-Ah Shim Jong-Ha Moon Byung-Teak Lee Jin hyeok Kim Photonic and Electronic Thin Film Laboratory Department of Materials Science and Engineering Chonnam National University 300 Yongbong-Dong Puk-Gu Kwangju 500-757 South Korea

Lead-based perovskite relaxor ferroelectric materials have been subject of extensive research in recent decades because of their excellent dielectric, electromechanical and electro-optic properties. In the Pb(BB) O fa...

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