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Gene characterization and phylogenetic analysis of four mitochondrial genomes in Caenogastropoda

Acta Oceanologica Sinica 2024年第2期43卷 137-150页

作者： Jiangyong Qu Wanqi Yang Xindong Teng Li Xu dachuan Zhang Zhikai Xing Shuang Wang Xiumei liu Lijun Wang Xumin Wang College of Life Science Yantai UniversityYantai 264005China Qingdao International Travel Healthcare Center Qingdao 266071China Qingdao Dagang Customs Qingdao 266071China

Caenogastropoda is a highly diverse group,containing~60%of all existing gastropods.Species in this subclass predominantly inhabit marine environments and have a high ecological and economic value.Owing to the increase in relevant phylogenetic studies,our understanding of between species relatedness in Caenogastropoda has improved.However,the biodiversity,taxonomic status,and phylogenetic relationships of this group remain unclear.In the present study,we performed next-generation sequencing of four complete mitochondrial genomes from three families(Buccinidae,Columbellidae,and Cypraeidae)and the four mitogenomes were classical circular structures,with a length of 16177 bp in Volutharpa ampullacea,16244 bp in Mitrella albuginosa,16926bp in Mauritia arabica asiatica and 15422 bp in Erronea errones.Base composition analysis indicated that whole sequences were biased toward A and T.Then compared them with 171 complete mitochondrial genomes of Caenogastropoda.The phylogenetic relationship of Caenogastropoda derived from Maximum Likelihood(ML)and Bayesian Inference(BI)trees constructed based on CDS sequences was consistent with the results of traditional morphological analysis,with all three families showing close relationships.This study supported Caenogastropoda at the molecular level as a separate clade of Mollusca.According to our divergence time estimations,Caenogastropoda was formed during the middle Triassic period(~247.2–237 Ma).Our novel mitochondrial genomes provide evidence for the speciation of Caenogastropoda in addition to elucidating the mitochondrial genomic evolution of this subclass.

关键词： mitochondrial genome phylogenetic analysis Caenogastropoda

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Early osteoimmunomodulation by mucin hydrogels augments the healing and revascularization of rat critical-size calvarial bone defects

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Bioactive Materials 2023年第7期25卷 176-188页

作者： Song Chen Huan Wang dachuan liu Jianzhong Bai Håvard Jostein Haugen Bin Li Hongji Yan Orthopedic Institute Department of Orthopedic SurgeryThe First Affiliated HospitalSchool of Biology&Basic Medical SciencesSuzhou Medical CollegeSoochow UniversitySuzhouJiangsuChina Department of Biomaterials Institute for Clinical DentistryUniversity of OsloPO Box 1109 BlindernOslo0376Norway AIMES-Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institute and KTH Royal Institute of Technology 17177StockholmSweden Department of Neuroscience Karolinska Institute17177StockholmSweden Division of Glycoscience Department of ChemistrySchool of Engineering Sciences in ChemistryBiotechnology and HealthKTH Royal Institute of TechnologyAlbaNova University Center10691StockholmSweden

The design principle of osteogenic bone grafts has shifted from immunological inertness to limiting foreign body response to combined osteoimmunomodulatory activity to promote high-quality endogenous bone regeneration.Recently developed immunomodulatory mucin hydrogels have been shown to elicit very low complement activation and suppress macrophage release and activation after implantation in vivo.However,their immunoregulatory activity has not yet been studied in the context of tissue repair.Herein,we synthesized mucin-monetite composite materials and investigated their early osteoimmunomodulation using a critical-size rat bone defect model.We demonstrated that the composites can polarize macrophages towards the M2 phenotype at weeks 1 and 2.The early osteoimmunomodulation enhanced early osteogenesis and angiogenesis and ultimately promoted fracture healing and engraftment(revascularization of the host vasculature)at weeks 6 and 12.Overall,we demonstrated the applicability of mucin-based immunomodulatory biomaterials to enhance tissue repair in tissue engineering and regenerative medicine.

关键词： Mucin hydrogels Monetite osteoimmunomodulation Bone Revascularization

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TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair

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Bioactive Materials 2023年第1期19卷 581-593页

作者： Qiang Wei dachuan liu Genglei Chu Qifan Yu Zhao liu Jiaying Li Qingchen Meng Weishan Wang Fengxuan Han Bin Li Department of Orthopaedic Surgery Orthopaedic InstituteThe First Affiliated HospitalSuzhou Medical CollegeSoochow UniversitySuzhouJiangsuChina Department of Orthopaedic Surgery The First Affiliated HospitalShihezi University School of MedicineShiheziXinjiangChina Collaborative Innovation Center of Hematology Soochow UniversitySuzhouJiangsuChina

Annulus fibrosus(AF)repair remains a challenge because of its limited self-healing ability.Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair.Although the unique and beneficial characteristics of decellularized extracellular matrix(ECM)in tissue repair have been demonstrated,the poor mechanical property of ECM hydrogels largely hinders their applications in tissue regeneration.In the present study,we combined polyethylene glycol diacrylate(PEGDA)and decellularized annulus fibrosus matrix(DAFM)to develop an injectable,photocurable hydrogel for AF repair.We found that the addition of PEGDA markedly improved the mechanical strength of DAFM hydrogels while maintaining their porous structure.Transforming growth factor-β1(TGF-β1)was further incorporated into PEGDA/DAFM hydrogels,and it could be continuously released from the hydrogel.The in vitro experiments showed that TGF-β1 facilitated the migration of AF cells.Furthermore,PEGDA/DAFM/TGF-β1 hydrogels supported the adhesion,proliferation,and increased ECM production of AF cells.In vivo repair performance of the hydrogels was assessed using a rat AF defect model.The results showed that the implantation of PEGDA/DAFM/TGF-β1 hydrogels effectively sealed the AF defect,prevented nucleus pulposus atrophy,retained disc height,and partially restored the biomechanical properties of disc.In addition,the implanted hydrogel was infiltrated by cells resembling AF cells and well integrated with adjacent AF tissue.In summary,findings from this study indicate that TGF-β1-supplemented DAFM hydrogels hold promise for AF repair.

关键词： Decellularized matrix Annulus fibrosus Hydrogel TGF-β1 Tissue repair

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Hematoma-like dynamic hydrogelation through natural glycopeptide molecular recognition for infected bone fracture repair

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Bioactive Materials 2023年第12期30卷 73-84页

作者： Shenghao Wang Wenbo He Huan Wang dachuan liu Miao Wang Huilin Yang Guoqing Pan Bin Li Orthopedic Institute Department of Orthopaedic SurgeryMedical 3D Printing CenterThe First Affiliated HospitalSchool of Biology&Basic Medical SciencesSuzhouMedical CollegeSoochow UniversitySuzhouJiangsu215006China Collaborative Innovation Center of Hematology Soochow UniversitySuzhouJiangsu215006China Institute for Advanced Materials School of Materials Science and EngineeringJiangsu UniversityZhenjiangJiangsu212013China

Infected bone fractures remain a major clinical challenge for orthopedic surgeons.From a tissue regeneration perspective,biomaterial scaffolds with antibacterial and osteoinductive activities are highly desired,while advanced materials capable of mimicking the pathological microenvironment during the healing process of infected tissues remain an area deserving more research.Hematoma,the gel-like blood coagulum,plays an essential role in bone fracture repair because of its ability to serve as a dynamic and temporary scaffold with cytokines for both pathogen elimination and tissue healing.In light of this,we designed a dynamic hydrogel with hematoma-like antimicrobial or reparative performance for infected bone fracture repair in this study.The proposed dynamic hydrogel network was based on the reversible recognition of a natural glycopeptide antibiotic vancomycin(Van)and its target dipeptide D-Ala-D-Ala(AA),which could serve as a hematoma-like scaffold for obliterating bacteria in the fracture region and promoting bone repair by introducing an endogenous osteogenic peptide(OGP).In vivo experiments demonstrated that the hydrogel could rapidly eradicate bacteria,improve bone regeneration and restore the local inflammatory microenvironment.Together,findings from this study imply that the use of hematoma-like dynamic hydrogel could lead to a biomimetic revolution in surgical strategies against susceptible bone fractures.

关键词： Dynamic biomaterial Molecular recognition Hematoma mimicking Infected fracture repair Inflammatory microenvironment restoration

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糖尿病视网膜病变进展的影响因素

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国际眼科杂志 2024年第6期24卷 943-949页

作者：赵颖刘大川首都医科大学宣武医院眼科北京市100053

目的:分析影响糖尿病视网膜病变(DR)进展的相关因素。方法:回顾性收集非增殖期糖尿病视网膜病变(NPDR)患者同时进行随访观察,将队列中由NPDR进展为增殖期糖尿病视网膜病变(PDR)的患者共77例作为队列中的疾病进展组,未进展为PDR的NPDR患... 详细信息

目的:分析影响糖尿病视网膜病变(DR)进展的相关因素。方法:回顾性收集非增殖期糖尿病视网膜病变(NPDR)患者同时进行随访观察,将队列中由NPDR进展为增殖期糖尿病视网膜病变(PDR)的患者共77例作为队列中的疾病进展组,未进展为PDR的NPDR患者共115例作为队列中的观察组进行巢式病例对照研究,比较NPDR与PDR两组一般资料及实验室指标;以一般资料及实验室指标作为自变量,PDR作为结局变量,对DR进展的影响因素进行回归分析;最终对DR进展的影响因素进行诊断试验评价。结果:PDR组较NPDR组年龄更小(P=0.001)、糖尿病病程更长(P=0.01)。实验室指标对比:PDR组中糖化血红蛋白(HbA1c)(P=0.001)、血尿素氮(BUN)(P=0.003)、血沉(ESR)(P<0.001)及同型半胱氨酸(HCY)(P=0.001)明显高于NPDR组,平均红细胞血红蛋白(MCH)(P=0.043)、平均红细胞血红蛋白浓度(MCHC)(P=0.002)明显低于NPDR组。影响DR进展的因素:HbA1c(OR=1.587,P<0.001),BUN(OR=1.456,P=0.008),MCH(OR=0.540,P=0.038),ESR(OR=1.122,P=0.005),HCY(OR=1.838,P=0.002)。采用ROC曲线分析确定DR进展影响因素的最佳诊断分界点:HbA1c为8.18%,BUN为5.46 mmol/L,ESR为8.93 mm/h, HCY为13.95μmol/L。结论:HbA1c、BUN、MCH、ESR、HCY是DR进展的影响因素,其中HbA1c、BUN、ESR、HCY是DR进展的危险因素,MCH是DR进展的保护因素。

关键词：糖尿病视网膜病变进展影响因素

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眼科住院医师规范化培训中神经眼科专业临床教学方法初探

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中国毕业后医学教育 2024年第3期8卷 191-193页

作者：李臻刘大川首都医科大学宣武医院眼科北京100053

神经眼科是一门新兴的交叉学科,其临床教学工作在国内刚刚起步。神经眼科因涉及多个学科,存在学习起点较高、学习曲线较长、教学难度较大的特点。基于此,临床教学工作中从基础入手、循序渐进,加强疾病诊断思路培养,采用神经眼科病例库... 详细信息

神经眼科是一门新兴的交叉学科,其临床教学工作在国内刚刚起步。神经眼科因涉及多个学科,存在学习起点较高、学习曲线较长、教学难度较大的特点。基于此,临床教学工作中从基础入手、循序渐进,加强疾病诊断思路培养,采用神经眼科病例库教学等多种教学方式,引入思政元素等方法,全面提升眼科住院医师岗位胜任力,获得了良好的教学效果。

关键词：神经眼科临床教学住院医师规范化培训课程思政岗位胜任力

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不同术式治疗退行性下睑内翻的临床效果分析

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中国中医眼科杂志 2024年第3期34卷 232-235,241页

作者：袁玮李臻刘大川首都医科大学宣武医院北京100053

目的分析2种不同术式治疗退行性下睑内翻的临床疗效。方法选取2017年3月—2020年9月在首都医科大学宣武医院诊治的退行性下睑内翻患者84例(86只眼)。按照随机数字表法分为加固组41例(42只眼)和前徙组43例(44只眼)。加固组予下睑缩肌加... 详细信息

目的分析2种不同术式治疗退行性下睑内翻的临床疗效。方法选取2017年3月—2020年9月在首都医科大学宣武医院诊治的退行性下睑内翻患者84例(86只眼)。按照随机数字表法分为加固组41例(42只眼)和前徙组43例(44只眼)。加固组予下睑缩肌加固联合睑板前轮匝肌缩短术;前徙组予下睑缩肌前徙联合外侧睑板条固定术。比较2组的手术时间、术后眼睑瘀血持续时长、复发率和临床疗效。结果(1)2组术前下睑前方牵拉试验、下睑侧方牵拉试验和下睑缩肌松弛试验阳性率比较,差异均无统计学意义(P>0.05)。(2)手术时间:加固组手术时间低于前徙组,差异有统计学意义(t=32.444,P=0.000)。(3)术后眼睑瘀血持续时长:加固组术后眼睑瘀血持续时长低于前徙组,差异有统计学意义(t=5.544,P=0.000)。(4)复发率和临床疗效:2组复发率和临床疗效比较,差异均无统计学意义(P>0.05),且术后均未出现严重并发症。结论下睑缩肌加固联合睑板前轮匝肌缩短术和下睑缩肌前徙联合外侧睑板条固定术均是治疗退行性下睑内翻的有效方法。但下睑缩肌加固联合睑板前轮匝肌缩短术具有手术步骤简单、手术用时短、术后眼睑瘀血轻等优点。

关键词：眼睑内翻退行性下睑缩肌眼轮匝肌外侧睑板条联合手术

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雷珠单抗联合半量睫状体光凝术治疗新生血管性青光眼的效果观察

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中国临床医生杂志 2024年第4期52卷 400-403页

作者：边俊杰张磊刘大川首都医科大学宣武医院眼科北京100053

目的探讨雷珠单抗联合半量睫状体光凝术治疗新生血管性青光眼(NVG)的效果观察。方法选取2021年1月至2023年1月在首都医科大学宣武医院治疗的NVG患者110例(110眼)作为研究对象。在组间基线特征匹配的原则上,按照简单随机数表法将入组NVG... 详细信息

目的探讨雷珠单抗联合半量睫状体光凝术治疗新生血管性青光眼(NVG)的效果观察。方法选取2021年1月至2023年1月在首都医科大学宣武医院治疗的NVG患者110例(110眼)作为研究对象。在组间基线特征匹配的原则上,按照简单随机数表法将入组NVG患者分为观察组与对照组,各55例。对照组行半量睫状体光凝术,观察组在其基础上加用雷珠单抗治疗每月1次,连续3次。比较两组新生血管消退时间、治疗成功率、术后并发症及6个月复发率,治疗后1个月、3个月和6个月最佳矫正视力(BCVA)和眼压恢复情况,及治疗前、治疗3个月后血清检测指标[血清白细胞介素-1β(IL-1β)、IL-6和血管内皮生长因子水平(VEGF)]。结果观察组患者新生血管消退时间及成功率优于对照组(P0.05);两组患者眼压均比治疗前明显降低,且观察组各时间点更低,差异有统计学意义(P0.05)。结论雷珠单抗联合半量睫状体光凝术治疗NVG成功率较高,可以有效改善眼压和炎症水平。

关键词：新生血管性青光眼雷珠单抗半量睫状体光凝术视力眼压

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形成性评价在眼科住院医师规范化培训中的应用

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继续医学教育 2024年第5期38卷 107-110页

作者：方薇高欣然刘大川首都医科大学宣武医院眼科北京100053

眼科住院医师规范化培训是毕业后医学教育的重要组成部分,首都医科大学宣武医院眼科不断改进对住院医师的管理及教学方法,在住院医师规范化培训专题讲座、教学查房和病例讨论等教学活动中,引入形成性评价,以《眼科培训细则》及推荐教材... 详细信息

眼科住院医师规范化培训是毕业后医学教育的重要组成部分,首都医科大学宣武医院眼科不断改进对住院医师的管理及教学方法,在住院医师规范化培训专题讲座、教学查房和病例讨论等教学活动中,引入形成性评价,以《眼科培训细则》及推荐教材为指导,确定学习及评价范围,将每月进行的教学活动内容形成理论知识测试卷,对研究对象进行闭卷考试。试卷评阅后逐一反馈给住院医师,同时对每道题完成情况进行分析,反馈给相关授课教师。通过一段时间的尝试,发现住院医师对教学活动中知识点的掌握情况明显提升。对参与的教师和住院医师进行问卷调查显示,均对此培养方式有较高的满意度。在眼科住院医师规范化培训教学活动理论授课中应用形成性评价,可以调动住院医师参加教学活动的积极性和主动性,提高专注度和自主学习的意愿,促进课后复习及相关文献书籍的学习,有助于理论知识的掌握,提高学习成绩。同时此法有助于教师及时调整授课内容和方法,提高教学效率,更好地达到教学目的。

关键词：形成性评价住院医师规范化培训眼科教学专题讲座教学查房病例讨论

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Simulating multiple urban land use changes by integrating transportation accessibility and a vector-based cellular automata:a case study on city of Toronto

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Geo-Spatial Information Science 2022年第3期25卷 439-456页

作者： Xiaocong Xu dachuan Zhang Xiaoping liu Jinpei Ou Xinxin Wu School of Geography and Planning Guangdong Key Laboratory for Urbanization and Geo-simulationSun Yat-Sen UniversityGuangzhouChina Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) ZhuhaiChina

The accessibility provided by the transportation system plays an essential role in driving urban growth and urban functional land use changes.Conventional studies on land use simulation usually simplified the accessibility as proximities and adopted the grid-based simulation strategy,leading to the insufficiencies of characterizing spatial geometry of land parcels and simulating subtle land use changes among urban functional types.To overcome these limita-tions,an Accessibility-interacted Vector-based Cellular Automata(A-VCA)model was proposed for the better simulation of realistic land use change among different urban functional types.The accessibility at both local and zonal scales derived from actual travel time data was considered as a key driver of fine-scale urban land use changes and was integrated into the vector-based CA simulation process.The proposed A-VCA model was tested through the simulation of urban land use changes in the City of Toronto,Canada,during 2012-2016.A vector-based CA without considering the driving factor of accessibility(VCA)and a popular grid-based CA model(Future Land Use Simulation,FLUS)were also implemented for compar-isons.The simulation results reveal that the proposed A-VCA model is capable of simulating fine-scale urban land use changes with satisfactory accuracy and good morphological feature(kappa=0.907,figure of merit=0.283,and cumulative producer’s accuracy=72.83%±1.535%).The comparison also shows significant outperformance of the A-VCA model against the VCA and FLUS models,suggesting the effectiveness of the accessibility-interactive mechanism and vector-based simulation strategy.The proposed model provides new tools for a better simula-tion of fine-scale land use changes and can be used in assisting the formulation of urban and transportation planning.

关键词： Urban land use change simulation transportation accessibility Vector-based cellular automata(VCA)

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