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multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 881-892页

作者： Stéphanie Lynseh Carine Sita Bénao dabiré amana métuor Abdoul Karim Ouattara Rahimatou Yasmine Wendkouni Tiemtoré Nicolas Ouédraogo Blandine Ouédraogo Rhaina Olivia Badini Lionel Eliada Benoit Bambara Serge Sougué Jacques Simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center (CERBA) Department of Biochemistry-Microbiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso Department of Biochemistry-microbiology University of D&#233dougou D&#233dougou Burkina Faso National malaria Research and Training Centre (CNRFP) Ouagadougou Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AmC), 23.57% aztreoname (ATm) and 27.14% were resistant to imipenem (ImP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AmC, 39.53% to ATm and finally 47.28% were resistant to ImP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： multidrug-Resistant Chicken Ouagadougou Escherichia coli Salmonella spp. Antibiotic

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Resistance of Klebsiella to Imipenem by Production of Carbapenemase Gene bla_ImP at Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso

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International Journal of Clinical medicine 2023年第8期11卷 347-356页

作者： Blandine Ouédraogo Abdoul Karim Ouattara amana mètuor dabiré Rahimatou Yasmine Wendkuni Tiemtoré Serge Sougé Jacques Simporé Laboratoire de Biologie moléculaire et de Génétique (LABIOGENE) UFR-SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso Université Norbert Zongo Centre Universitaire de Manga Koudougou Burkina Faso Département de Biochimie-microbiologie Université de Dédougou Dédougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso

Objective: Class B carbapenemases are bacterial enzymes that catalyze the hydrolysis of β-lactam core antibiotics, except for monobactams. The objective of this study was to identify the carbapenemase gene blaImP in the genus Klebsiella at the Charles De Gaulle Pediatric University Hospital (CHUP-CDG) of Ouagadougou, Burkina Faso. methods: The study involved 17 bacterial strains responsible for human infection and isolated from various biological samples during the period from 2009 to 2013. The strains were tested for antimicrobial susceptibility to cefotaxime, ceftazidime and imipenem using the mueller-Hinton agar diffusion method. The carbapenemases resistance genes were detected by conventional PCR using specific primers at the molecular biology laboratory of CERBA/LABIOGENE, Ouagadougou, Burkina Faso. Results: The antibiotic susceptibility test showed high resistance of the 17 Klebsiella isolates tested to cephalosporins. A high cefotaxime-resistance rate (82.35%) and ceftazidime-resistance rate (88.23%) was found among the strains tested against 11.76% resistance rate for imipenem. Analysis of PCR products by gel electrophoresis revealed 4 strains (23.53%) with blaImP-type gene. Conclusion: Klebsiella is a well-known bacterium in clinical practice. The present study demonstrated the blaImP-type gene in cephalosporin-resistant strains of Klebsiella at CHUP-CDG. more effective monitoring and treatment solutions are needed to prevent the spread of these resistant strains.

关键词： Klebsiella Resistance blaImPGenes β-lactam Burkina Faso

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molecular Detection of Extended-Spectrum Beta Lactamase and Carbapenemase Genes in Escherichia coli and Salmonella spp. Isolated from Chickens Consumed Outdoors in Ouagadougou, Burkina Faso

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Journal of Biosciences and medicines 2024年第11期12卷 221-232页

作者： amana mètuor dabiré Stéphanie Lynseh Carine Sita Bénao Nicolas Ouédraogo Rahimatou Yasmine Wendkouni Tiemtoré Rhaina Olivia Badini Lionel Eliada Benoit Bambara Jacques Simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center Department of Biochemistry-Microbiology Joseph Ki-Zerbo University Ouagadougou Burkina Faso Department of Biochemistry-microbiology University of Ddougou Ddougou Burkina Faso Centre National de Recherche et de Formation sur le Paludisme Institut National de Sant Publique Ouagadougou Burkina Faso Laboratoire de biochimie et immunologie applique (LABIA) Joseph Ki-Zerbo University Ouagadougou Burkina Faso

This study was carried out to assess the prevalence of resistance genes in strains of Escherichia coli and Salmonella spp. isolated from free-range chickens in Ouagadougou, where resistant bacteria can be transmitted to humans via faeces or contaminated meat. A total of 280 strains of Escherichia coli and 129 strains of Salmonella spp. resistant to at least one beta-lactam or carbapenem antibiotic were used in this study. PCR analyses revealed the presence of ESBL (extended spectrum beta lactamase) resistance genes in Escherichia coli isolates, with 3.21% (9/280) possessing the CTX-m (Cefotaximase) gene, 15.35% (43/280) had the SHV (Sulfhydril Variable) gene, and 11.42% (32/280) had carbapenemase resistance genes, more specifically ImP (Imipenemase metallo-beta-lactamase). As regards Salmonella spp. strains, only the presence of the SHV (Sulfhydril Variable) gene was identified in 2.32% (3/129) of isolates belonging to the ESBL family, while 26.35% (34/129) and 13.95% (18/129) of isolates respectively possessed the ImP (Imipenemase metallo-beta-lactamase) and NDm (New Delhi metallo-β-lactamase) genes, both of the carbapenemase type. The significant prevalence of resistance genes in bacterial strains isolated from chickens sold outdoors in Ouagadougou raises major public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： Bacteria Resistance Extended-Spectrum Beta Lactamases Carbapenemases Ouagadougou

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microbiological Quality of “Rabilé”, a Yeast Used for Fermentation of Dolo, a Local Beer in Dédougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 849-864页

作者： amana metuor dabiré Nicolas Ouédraogo Cheick Alassane Djibila Damis Yves Patrik Bouniounou molecular Biology and Genetics Laboratory (LABIOGENE) Universit&#233 Joseph Ki-Zerbo Ouagadougou Burkina Faso Training and Research Unit in Applied Sciences and Technologies Department of Biochemistry Microbiology University of Dedougou Dedougou Burkina Faso National Center for Research and Training on malaria (CNRFP) Ouagadougou Burkina Faso

Sorghum beer or dolo is part of the eating habits of part of the population of Dédougou because of its low price compared with industrial beers. Its production is an ancestral tradition that uses traditional equipment and gives dolo organoleptic properties that are not found in industrial beers. The production process involves several stages, including fermentation, which itself comprises natural lactic fermentation followed by alcoholic fermentation using traditional yeasts, which are not controlled in any way. The general aim of this study is to assess the microbiological quality of these fermentative yeasts in the town of Dédougou, in order to contribute to the health safety of the population and the promotion of these local beers. Twenty samples of fermenting yeast were analyzed according to ISO standards, to isolate enterobacteria, total and faecal coliforms according to standard procedures for isolating these micro-organisms. The isolated strains were identified using the API20E gallery. microbiological analyses revealed the presence of 51.17% enterobacteria, 45.38% total coliforms and 3.45% thermotolerant coliforms. We counted 40% Escherichia coli, 20% Enterobacter cloacae, 20% Klebsiella pneumoniae and 20% Klebsiella spp. All the strains detected are capable of surviving in hostile conditions and could harm the quality of the dolo, consumer health and cause real collective food poisoning in the town of Dédougou. This enabled us to assess the microbial quality of these yeasts and to propose more suitable measures for producing and preserving dolo under hygienic conditions to protect consumer health.

关键词： Dolo Yeast Rabilé microbiological Quality Bacteria

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Frequency of Antibiotic Resistance of Escherichia coliand Klebsiella pneumoniaeby Production of TOHO-Type β-Lactamases at Saint Camille Hospital, Ouagadougou (Burkina Faso)

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Advances in microbiology 2021年第12期11卷 713-722页

作者： Serge Sougué amana mètuor-dabiré Djénéba Ouermi Yasmine Rahimatou Wend-Kouni Tiemtoré Bénao Stéphanie Lynseh Carine Sita Théodora mahoukèdè Zohoncon Yasmine Aminata Bangré Elie Kabré Jacques Simporé Department of Biochemistry and microbiology Faculty of Applied Science and Technology Biomolecular Research Centre (CERBA/LABIOGENE) UFR-SVT Joseph KI-ZERBO University Ouagadougou Burkina Faso National Public Health Laboratory of Ouagadougou Ouagadougou Burkina Faso University of Dedougou Dedougou Burkina Faso Saint Thomas Aquinas University (USTA) Saint Camille Hospital of Ouagadougou Ouagadougou Burkina Faso

Extended-spectrum β-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEm, blaCTX-m and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-m subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in Escherichia coli and Klebsiella pneumoniae in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by Escherichia coli and Klebsiella pneumoniae using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value Escherichia coli and 18 (46.2%) Klebsiella pneumoniae. molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in Escherichia coli and Klebsiella pneumoniae.

关键词： Antibiotic Resistance Bacteria ESBL Genes TOHO

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Assessment of radioactivity levels in some cement produced locally in Iraq

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Radiation Detection Technology and methods 2021年第4期5卷 633-640页

作者： maitham S.amana Najwa J.Jubier mohammed J.R.Aldhuhaibat A.A.Salim Department of Physics College of ScienceUniversity of WasitKutWasitIraq Laser Center&Physics Department Faculty of ScienceUniversiti Teknologi MalaysiaJohor bahruJohorMalaysia

Purpose Due to the rapid expansion of the construction industries,cement production is ever-increasing worldwide and maintaining the qualities of the local cement became vital for safe and durable *** this regard,the radiometric assessment of cement plays a significant *** The specific activities of the naturally occurring radionuclides present in ten Portland cement samples that can cause possible health hazards were determined using a gamma-ray spectrometer equipped with(3×3)inch NaI(Tl)detector with 1024 *** and conclusion The measured activity concentrations of Ra-226,Th-232,and K-40 were found in the range of 12.38–55.75(average of 31.29 Bq/kg),6.29–57.54(average of 15.97 Bq/kg),and 112.63–266.37(average of 168.24 Bq/kg),*** recorded average activity concentration of these radionuclides in the cement was below the stipulated world standard by UNSCEAR(Sources and Effects of Ionizing Radiation,United Nations Scientific Committee on the Effects of Atomic Radiation UNSCEAR 2000 Report to the General Assembly,with Scientific Annexes,vol.I,2000).The obtained mean activity concentration of Raeq in the cement(67.101 Bq/kg)was much lower than the recommended guideline of 370 Bq/*** addition,the mean value of the calculated absorbed dose rate(59.947 nGy·h^(−1)),annual effective dose(0.220 mSv·y^(−1)),activity concentration index(0.240),alpha index(0.156),excess lifetime cancer risk(0.771×10^(−3))external(0.084)and internal(0.300)hazard index of the studied samples were much below the recommended safety *** is affirmed that the cement sold in the local market can safely be used for building constructions.

关键词： Cement Concentration Absorbed dose Alpha index Health hazards

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非角膜地形图引导PRK联合角膜胶原交联术治疗圆锥角膜（英文）

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国际眼科杂志 2019年第3期19卷 358-362页

作者： Suzan amana Rattan Seif Alshamarti Khalil m Al-Salem mahir kadhim mutashar 巴格达大学 Al kindy医学院伊拉克158001 Alqadisiyah大学医学院伊拉克258001 mutah大学医学院眼科约旦alkarak361710

目的:评估非角膜地形图引导圆锥角膜患者行光折变角膜切除术(PRK)和角膜胶原交联术(CXL)的视力、屈光度和临床疗效。方法:术后1mo, 3mo, 6mo and 12mo对34例患者未矫正视力(UDVA)和矫正距离视力(CDVA),平、陡角膜测量读数以及并发症进... 详细信息

目的:评估非角膜地形图引导圆锥角膜患者行光折变角膜切除术(PRK)和角膜胶原交联术(CXL)的视力、屈光度和临床疗效。方法:术后1mo, 3mo, 6mo and 12mo对34例患者未矫正视力(UDVA)和矫正距离视力(CDVA),平、陡角膜测量读数以及并发症进行评估。结果:共34例患者平均年龄为23.3±4.0岁。UDVA和CDVA显著提高,且术后1a恢复平稳。通过超过1a的定期随访,T检验显示术前术后值有显著不同(Pmo达到稳定状态,与非角膜地形图引导PRK相比,地形图引导的唯一优势可能是通过Fourier术后分析,在某些患者中,球镜和柱镜轴位漂移。

关键词：圆锥角膜屈光手术屈光性角膜磨镶术角膜胶联疗法非角膜地形图引导屈光性角膜切除术近视散光

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Phytochemical and Antifungal Activity of Leaf Extracts of Prosopis africana and Anacardium occidentale against macrophomina Root Rot of Sesamum indicum L. in Benue State, Central Nigeria

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Journal of Geoscience and Environment Protection 2018年第7期6卷 66-76页

作者： matthew Elaigwu Hyacinth Ocheigwu Apeh Oluma amana Onekutu Department of Integrated Science College of Education Oju Benue State Nigeria Department of Biological Sciences University of Agriculture Makurdi Benue State Nigeria

This study investigated the antifungal activity of leaf extracts of Prosopis africana and Anacardium occidentale against macrophomina phaseolina, the causal agent of root rot of Sesamum indicum L. Phytochemical analysis of the two plants showed the presence of alkaloids, saponins, tannins, flavonoids and anthraquinones in petroleum ether, ethyl acetate, methanol and water extracts. The effectiveness of the two medicinal plants viz: P. africana and A. occidentale was tested against the causative agent of root rot of Sesamum indicum L. The effect of plant leaf extracts on mycelia growth of the test organism shows that both P. africana and A. anacardium reduced the mycelia growth significantly as compared to the control (plate, 2, 3, 4). The antifungal property of P. africana and A. occidentale makes these plants of potential interest for the control of the fungi macrophomina phaseolina.

关键词： Phytochemical Antifungal Screening Prosopis africana Anacardium occidentale Leaf Extracts macrophomina Phaseolina (Tassi) Goid

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前房注射0.1%莫西沙星联合结膜下注射曲安奈德预防超声乳化术后感染（英文）

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国际眼科杂志 2018年第10期18卷 1757-1762页

作者： Suzan amana Rattan Najah Kadhum mohammad mahir Kadhim mutashar 巴格达大学Al-kindy医学院手术室伊拉克巴格达AL-Nahdhasquare10001 巴格达大学医学院手术室伊拉克巴格达BabAL-Moadhamsquare10001 Al-Wazyrea 眼专科私立医院伊拉克巴格达10001

目的:评估前房注射0.1%莫西沙星联合结膜下注射4 mg曲安奈德预防白内障超声乳化术后感染和炎症的疗效,并与局部滴药组比较。方法:共纳入1000例,平均年龄38～70岁,全部行超声乳化术的患者分为两组,两组在年龄、术前眼压(IOP)和中心黄斑厚... 详细信息

目的:评估前房注射0.1%莫西沙星联合结膜下注射4 mg曲安奈德预防白内障超声乳化术后感染和炎症的疗效,并与局部滴药组比较。方法:共纳入1000例,平均年龄38～70岁,全部行超声乳化术的患者分为两组,两组在年龄、术前眼压(IOP)和中心黄斑厚度(CmT)方面无显著差异,分别为P=0.6,P=0.9和P=0.8。对照组患者500例,术后1mo每日4次局部应用0.5%盐酸莫西沙星和0.1%地塞米松滴眼液。研究组在手术结束时,前房注入0.1%莫西沙星0. 4 m L联合4 mg曲安奈德结膜下注射。随访时间为术后1d、1wk、1mo和3mo。检查4次前房反应,测量3次眼压,最后一次测量CmT。结果:该临床试验研究中两组人群分别应用不同的预防方法,两组无眼内炎病例发生。通过两样本t检验,术后1d,研究组前房细胞显著低于对照组,而术后1wk、1mo和3mo两组间无统计学差异。在第3mo随访时,两组间眼压和中心黄斑厚度无明显差异。对照组炎症发生率为9.6%,研究组为4%。对照组在1mo时眼压升高≥10 mmHg患者占2. 4%,显著高于研究(0.8%)。结论:白内障术后联合应用前房内莫西沙星和结膜下曲安奈德可有效预防感染和炎症,并且大多数(480例)患者不需其它任何外用药物,这为患者节省了成本,并帮助降低无法实施局部用药的病人并发症的几率与依从性差的患者术后不良药物反应。

关键词：盐酸莫西沙星曲安奈德超声乳化

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Effect of Light Emitting Diodes (LEDs) on Growth, mineral Composition, and Nutritional Value of Wheat & Lentil Sprouts

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1117-1128页

作者： Abdul momin amana Khatoon Wajahat Khan Dilsat Bozdoğan Konuşkan muhammad mudasar Aslam muhammad Jamil Shafiq Ur Rehman Baber Ali Alevcan Kaplan Sana Wahab muhammad Nauman Khan Sezai Ercisli mohammad Khalid Al-Sadoon Department of Botany Kohat University of Science&TechnologyKohat26000Pakistan Department of Food Engineering Faculty of AgricultureHatay Mustafa Kemal UniversityHatay31000Türkiye Department of Botany University of Science&TechnologyBannu28100Pakistan Department of Biotechnology and Genetic Engineering Kohat University of Science&TechnologyKohat26000Pakistan Department of Biology University of HaripurKhyber Pakhtunkhwa22620Pakistan School of Science Western Sydney UniversityPenrithNew South Wales2151Australia Department of Crop and Animal Production Sason Vocational SchoolBatman UniversityBatman72060Türkiye Department of Plant Sciences Quaid-i-Azam UniversityIslamabad45320Pakistan Department of Botany Islamia College PeshawarPeshawar25120Pakistan Department of Horticulture Agricultural FacultyAtaturk UniversityErzurum25240Türkiye HGF Agro Ata TeknokentErzurumTR-25240Türkiye Department of Zoology College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia

Sprouts are ready-to-eat and are recognized worldwide as functional components of the human *** advances in innovative agricultural techniques could enable an increase in the production of healthy *** use of light-emitting diode(LED)in indoor agricultural production could alter the biological feedback loop,increasing the functional beneﬁts of plant foods such as wheat and lentil sprouts and promoting the bioavailability of *** effects of white(W),red(R),and blue(B)light were investigated on the growth parameters and nutritional value of wheat and lentil *** the laboratory,seeds were sown under three different LED treat-ments:white,red,and blue light,while normal incandescent light served as a *** seed germina-tion improved by 18.34%and 12.67%for wheat and 18.34%and 12.67%for lentil sprouts under LED treatments R and B,*** increase in total soluble protein and sugar by 33.4%and 9.23%in wheat and by 31.5%and 5.87%in lentils was observed under the R LED *** C concentrations in wheat and lentils were signiﬁcantly increased by R LED compared to all other *** parameters,including potassium and sodium concentrations,were signiﬁcantly increased under red and blue light compared to the control;white light,on the other hand,signiﬁcantly decreased all these *** to the experimental data,red and blue LED light could be beneﬁcial in the production of functional wheat and lentil sprouts with high nutrient concentrations.

关键词： Growth parameters LED nutritional value sprouts total soluble proteins

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