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Saline conducted electric coagulation (SCEC):original experience in experimental hepatectomy

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第3期13卷 186-191页

作者： Guo-ping DING Li-ping CAO da-ren liu Ri-sheng QUE Department of General Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective: To evaluate the feasibility and superiority of a new coagulating and hemostatic method named "saline conducted electric coagulation (SCEC)". Methods: The Peng's multifunction operative dissector (PMOD) was modified to enable saline to effuse persistently out of its nib at a constant speed. In a group of six New Zealand rabbits, two hepatic lobes of each rabbits were resected respectively by SCEC and conventional electric coagulation (EC). The features of SCEC were recorded by photo and compared with conventional EC. After 7 d, the coagulating depth was measured in each residual hepatic lobe. Hepatic tissue was dyed by hematoxylin and eosin (HE) and studied under a microscope. Results: The coagulating depth increased with the continuation of SCEC time. Hepatectomies were performed successfully, no rabbit died in the perioperative period. The incisal surface of SCEC was gray-white with no red bleeding point. There was a thick solidified layer at the margin and a thin red-white intermittent layer between the solidified layer and normal hepatic tissue at the vertical section of SCEC. The mean coagulating depth of SCEC was 1.8 cm vs. 0.3 cm of conventional EC. Pathological examination showed a mild inflammatory reaction by SCEC. Conclusions: SCEC is a feasible and safe method for surgical hemostasis. As a new technique for liver resection, SCEC shows better coagulating effect and milder inflammatory reaction than conventional EC. Our study shows bloodless liver resection can also be performed by SCEC, especially for liver malignant tumor.

关键词： Saline Electric coagulation Hepatectomy Hemostasis Bloodless liver resection

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Phlegmonous gastritis after esophagectomy:A case report

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World Journal of Gastroenterology 2013年第8期19卷 1330-1332页

作者： Jun-Qiang Fan da-ren liu Chao Li Gang Chen Department of Thoracic Surgery Second Affiliated HospitalSchool of MedicineZhejiang University Department of General Surgery Second Affiliated HospitalSchool of MedicineZhejiang University

Phlegmonous gastritis is an unusual infection of the gastric wall,which is extremely rare and associated with a poor ***,we report the case of a 65-year-old male patient with a history of splenectomy,who had phlegmonous gastritis after *** tomography revealed a remarkably distended thoracic stomach,and the gastric wall was locally *** mucosa was red and white in color and significantly edematous on *** was successfully treated with a combination of antibiotics and povidone-iodide intraluminal *** addition to this case,the clinical presentations,imaging examinations as well as treatments of phlegmonous gastritis are discussed.

关键词： Phlegmonous gastritis Esophagectomy Splenectomy Computed tomography

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Hem-o-lok clip migration to the common bile duct after laparoscopic common bile duct exploration:A case report

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World Journal of Clinical Cases 2022年第19期10卷 6548-6554页

作者： da-ren liu Jin-Hong Wu Jiang-Tao Shi Huan-Bing Zhu Chao Li Department of Hepatobiliary Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009Zhejiang ProvinceChina

BACKGROUND Laparoscopic cholecystectomy(LC)and laparoscopic common bile duct exploration(LCBDE)has been widely used for management of gallbladder and common bile duct(CBD)***-operative clip migration is a rare complication of laparoscopic biliary surgery,which can serve as a nidus for stone formation and cause recurrent *** SUMMARY A 59-year-old female was admitted to hospital because of fever and acute right upper abdominal *** has a history of LC and had a LCBDE surgery 2 mo *** examination revealed tenderness in the upper quadrant of right *** tomography scan demonstrated a high-density shadow at the distal CBD,which was considered as migrated *** speculation was confirmed by endoscopic retrograde cholangiopancreatography examination,and two displaced Hem-o-lok clips were removed with a stone *** fever or abdominal pain presented after the *** addition to the case report,literature regarding surgical clip migration after laparoscopic biliary surgery was reviewed and *** Incidence of postoperative clip migration may be reduced by using clips properly and correctly;however,new methods should be explored to occlude cystic duct and *** a patient with a past history of LC or LCBDE presents with features of sepsis and recurrent upper quadrant pain,clip migration must be considered as one of the differential diagnosis.

关键词： Laparoscopic cholecystectomy Laparoscopic common bile duct exploration Surgical clip Postoperative migration Case report

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Systematic review of laparoscopy-assisted versus open gastrectomy for advanced gastric cancer

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 468-478页

作者： Long-yun YE da-ren liu Chao LI Xiao-wen LI Ling-na HUANG Sheng YE Yi-xiong ZHENG Li CHEN Department of Surgery the Second Affiliated HospitalSchool of MedicineZhejiang University

Objective:The study compared laparoscopy-assisted gastrectomy(LAG) with open gastrectomy(OG) in the management of advanced gastric cancer(AGC).Methods:Literature search was performed in the Medline,Embase,and Cochrane Library databases to identify control studies that compared LAG and OG for AGC.A meta-analysis was conducted to examine the surgical safety and oncologic adequacy,using the random-effect ***:Seven eligible studies including 815 patients were *** was associated with less blood loss,less use of analgesics,shorter time of flatus and periods of hospital stay,but longer time of *** incidence of most complications was similar between the two ***,LAG was associated with a lower rate of pulmonary infection(odds ratio(OR) 0.19;95% confidence interval(CI) 0.05 to 0.68;Prences were noted in terms of the number of harvested lymph nodes(weighted mean difference(WMD) 1.165;95% CI-2.000 to 4.311;P>0.05),overall mortality(OR 0.65;95% CI 0.39 to 1.10;P>0.05),cancer-related mortality(OR 0.64;95% CI 0.32 to 1.25;P>0.05),or recurrence(OR 0.62;95% CI 0.33 to 1.16;P>0.05).Conclusions:LAG could be performed safely for AGC with adequate lymphadenectomy and has several short-term advantages compared with conventional *** differences were found in long-term ***,these results should be validated in large randomized controlled studies(RCTs) with sufficient follow-up.

关键词： Gastric cancer Laparoscopic gastrectomy Meta-analysis Mortality Recurrence

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On entire solutions of some Fermat type differential-difference equations

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Applied Mathematics(A Journal of Chinese Universities) 2024年第1期39卷 69-88页

作者： LONG Jian-ren QIN da-zhuan School of Mathematical Sciences Guizhou Normal UniversityGuiyang 550025China

On one hand,we study the existence of transcendental entire solutions with finite order of the Fermat type difference *** the other hand,we also investigate the existence and growth of solutions of nonlinear different... 详细信息

关键词： entire solutions differential-difference equations existence finite order

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Regulating ion transport behaviors toward dendrite-free potassium metal batteries:recent advances and perspectives

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Rare Metals 2024年第4期43卷 1435-1460页

作者： Lu-Kang Zhao Xuan-Wen Gao Tian-Zhen ren da Wang da-Wei Wang Zhao-Meng liu Hong Chen Wen-Bin Luo School of Metallurgy Northeastern UniversityShenyang110819China School of Chemical Engineering The University of New South WalesSydneyNSW2052Australia

With abundant potassium resources and high capacity,potassium metal batteries(PMBs)present a compelling option for the next generation of energy storage ***,PMBs suffer from an unstable anode interface caused by uncontrolled dendrite growth,which results in unsatisfactory cyclability and safety *** investigations suggest that significant progress has been made in enhancing the interfacial stability of *** various effective strategies for stabilizing interfaces can ultimately be attributed to the regulation of the sluggish ion transfer kinetics and irregular deposition,i.e.,the arrangement of ion transport behaviors at the *** modulation of ions transport rate and ions deposition directions makes it possible to obtain a dendrite-free and smooth deposition ***,the influencing factors and action mechanism of K^(+)interface transport behaviors are discussed to understand the nature of material design for constructing stable anode interfaces,including regulating the solvation and desolvation structures,accelerating K^(+)transport kinetics and controlling K^(+)deposition *** addition,the deficiencies and prospects of the research on electrolyte,separators and designed electrode involved in the manufacturing and testing and ion transport process of PMBs are *** review is expected to provide some possible directions for constructing dendrite-free interfaces in advanced PMBs-related research and offer significant insights for prospective experimental research and commercial applications.

关键词： Ion transport rate Ion transport direction Interface Potassium metal batteries Dendrites growth

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Influence of up-regulation of Notch ligand DLL4 on biological behaviors of human gastric cancer cells

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World Journal of Gastroenterology 2013年第28期19卷 4486-4494页

作者： Guo-Gang Li Lan Li Chao Li Long-Yun Ye Xiao-Wen Li da-ren liu Qi Bao Yi-Xiong Zheng da-Peng Xiang Li Chen Jian Chen Department of Surgery Second Affiliated Hospital College of Medicine Zhejiang University Department of Gastroenterology FirstAffiliated Hospital College of Medicine Zhejiang University

AIM: To investigate the potential roles of Delta-like ligand 4 (DLL4) on the biological behavior of gastric cancer cells and its molecular mechanisms. METHODS: A recombinant eukaryotic expression vector containing human DLL4 gene was constructed and transfected into the human gastric cancer cell line SGC7901. Clones with up-regulated DLL4 were selected and amplified. The effect of DLL4 up-regulation on gastric cancer cell growth was assessed using cell growth assay. The migration and invasion were assessed using a transwell migration assay and matrigel invasion assay. Matrix metalloproteinases were detected using the zymogram technique. Cells were implanted subcutaneously into male BALB/c nu/nu mice. Tumor volumes were then calculated and compared. DLL4 staining in the implanted tumor was performed using immunohistochemistry technique. RESULTS: Growth curves over a six-day time course showed significantly promoted cell proliferation of SGC7901 cells with up-regulated DLL4. DLL4 up-regulation in SGC7901 cells promoted the migration (205.4 ± 15.2 vs 22.3 ± 12.1, P < 0.05) and invasion (68.8 ± 5.3 vs 18.2 ± 6.0, P < 0.05) in vitro and tumorigenicity in vivo (2640.5 ± 923.6 mm 3 vs 1115.1 ± 223.8 mm 3 , P < 0.05). Furthermore, significantly increased mRNA level and increased secretion of matrix metalloproteinase-2 (MMP-2) proenzyme were observed in SGC7901 cells with up-regulated DLL4. However, increased MMP-9 mRNA level but decreased extracellular MMP-9 proenzyme level was observed. CONCLUSION: Our observations indicated a mechanism by which activation of DLL4-mediated Notch signaling promotes the expression and secretion of MMP-2 proenzyme and influences the progress of gastric cancer.

关键词： Gastric cancer Delta-like ligand 4/Notch Matrix metalloproteinase Migration Invasion

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Global Solvability for a Predator-Prey Model with Prey-Taxis and Rotational Flux Terms

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Chinese Annals of Mathematics,Series B 2024年第2期45卷 297-318页

作者： Guoqiang ren Bin liu School of Mathematics and Statistics Huazhong University of Science and Technology Hubei Key Laboratory of Engineering Modeling and Scientific Computing Huazhong Universityof Science and Technology

In this paper the author investigates the following predator-prey model with prey-taxis and rotational?ux terms■in a bounded domain with smooth *** presents the global existence of generalized solutions to the model... 详细信息

关键词： Predator-prey Prey-taxis Giopal existence Kotational flnux

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Anatomic isolated caudate lobectomy: Is it possible to establish a standard surgical flow?

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World Journal of Gastroenterology 2017年第41期23卷 7433-7439页

作者： Yun Jin Liang Wang Yuan-Quan Yu Dong-Er Zhou da-ren liu Jun-Jie Yang Shu-You Peng Jiang-Tao Li Department of General Surgery The Second Affiliated Hospital College of MedicineZhejiang University Department of General Surgery Xinchang People’s Hospital

AIM To establish the surgical flow for anatomic isolated caudate lobe resection. METHODS The study was approved by the ethics committee of the Second Affiliated Hospital Zhejiang University School of Medicine(SAHZU). From April 2004 to July 2014, 20 patients were enrolled who underwent anatomic isolated caudate lobectomy at SAHZU. Clinical and postoperative pathological data were analyzed. RESULTS Of the total 20 cases, 4 received isolated complete caudate lobectomy(20%) and 16 received isolated partial caudate lobectomy(80%). There were 4 caseswith the left approach(4/20, 20%), 6 cases with the right approach(6/20, 30%), 7 cases with the bilateral combined approach(7/20, 35%), 3 cases with the anterior approach(3/20, 15%), and the hanging maneuver was also combined in 2 cases. The median tumor size was 5.5 cm(2-12 cm). The median intraoperative blood loss was 600 m L(200-5700 m L). The median intra-operative blood transfusion volume was 250 m L(0-2400 m L). The median operation time was 255 min(110-510 min). The median post-operative hospital stay was 14 d(7-30 d). The 1-and 3-year survival rates for malignant tumor were 88.9% and 49.4%, respectively. CONCLUSION Caudate lobectomy was a challenging procedure. It was demonstrated that anatomic isolated caudate lobectomy can be done safely and effectively.

关键词： Caudate lobectomy Surgical flow Anatomic liver resection

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Computational Strategies and Algorithms for Inferring Cellular Composition of Spatial Transcriptomics data

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Genomics, Proteomics & Bioinformatics 2024年第3期22卷 1-9页

作者： Xiuying liu Xianwen ren Changping Laboratory Beijing 102206China

Spatial transcriptomics technology has been an essential and powerful method for delineating tissue architecture at the molecular ***,due to the limitations of the current spatial techniques,the cellular information cannot be directly measured but instead spatial spots typically varying from a diameter of 0.2 to 100µm are ***,it is vital to apply computational strategies for inferring the cellular composition within each spatial *** main objective of this review is to summarize the most recent progresses in estimating the exact cellular proportions for each spatial spot,and to prospect the future directions of this field.

关键词： Spatial transcriptomics Single-cell sequencing Cellular composition Spot deconvolution Cell type decomposition

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