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Chinese Chemical Letters 2008年第5期19卷 589-591页

作者： Wei Zhang Tao Yang da ming huang Kui Jiao College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao 266042 China

A novel electrochemical DNA biosensor based on zinc oxide （ZnO） nanoparticles and multi-walled carbon nanotubes （MWNTs） for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite film modified glassy carbon electrode （MWNTs/ZnO/CHIT/GCE） was fabricated and DNA probes were immobilized on the electrode surface. The hybridization events were monitored by differential pulse voltammetry （DPV） using methylene blue （MB） as an indicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the transgenic corn, with a detection limit of 2.8× 10^-12 mol/L of target sequence.

关键词： ZnO nanoparticles Multi-walled carbon nanotubes Chitosan DNA sensor PAT gene

Molecular Genetic Variation in a Clonal Plant Population of Leymus chinensis （Trin.） Tzvel.

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Journal of Integrative Plant Biology 2005年第9期47卷 1055-1064页

作者： Yu-Sheng WANG Li-ming ZHAO Hua WANG Jie WANG da-ming huang Rui-Min HONG Xiao-Hua TENG Nakamura MIKI Grassland Research Station College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China The School of Management Tianjin University Tianjin 300071 China School of Economy-Management Tianjin University of Technology Tianjin 300191 China Department of Breast Surgery Cancer Institute Fu-Dan University Medical Center Shanghai 200032 China Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China

Randomly amplified polymorphic DNA （RAPD） analysis was used to investigate the genetic variation among populations, between populations, and within populations, relationships between genetic distance and geographic distance, and the molecular variation and population size. The effects of geographic and genetic distances, as well as of genetic differentiation and population size, on genetic variations of Leymus chinensis （Trin.） Tzvel. are discussed. The present study showed that there was significant RAPD variation between the Baicheng region population and the daqing region population, with a molecular variance of 6.35% （P 〈 0.04）, and for differentiation among area populations of the daqing region, with a molecular variance of 8.78% （P 〈 0.002）. A 21.06% RAPD variation among all 16 populations among two regions was found （P 〈 0.001）, as well as 72.59% variation within populations （P 〈 0.001）. Molecular variation within populations was significantly different among 16 populations.

关键词： clonal plant population genetic distance genetic variation geographic distance molecular variation.

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宽温低损耗单模光纤定向耦合器

光学学报 1989年第1期9卷 93-96页

作者：陆善达冯明和黄勇上海科学技术大学物理系

本文报道了用熔融法由常规的单模通信光纤制造宽温工作的低损耗单模光纤定向耦合器.工作波长为1.52μm,分束比为50%的封装器件;工作温度在-30～+70℃范围,分束比容差小于±5%,插入损耗40dB.工作波长的变化对分束比影响十分明显,但入射... 详细信息

本文报道了用熔融法由常规的单模通信光纤制造宽温工作的低损耗单模光纤定向耦合器.工作波长为1.52μm,分束比为50%的封装器件;工作温度在-30～+70℃范围,分束比容差小于±5%,插入损耗40dB.工作波长的变化对分束比影响十分明显,但入射光的偏振态对其无实际影响.

关键词：单模光纤定向耦合器熔融法

Surface micro-arrays enhancing electromagnetic interference shielding of polydimethylsiloxane/multiwalled carbon nanotubes nanocomposites

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Nano Research 2025年第1期18卷 508-519页

作者： Ke Jiang Zhi Chen Ji Yu Chen Zheng Shan huang ming Wang Chongqing Key Laboratory of Soft-Matter Material Manufacturing School of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China School of Mechanics Civil Engineering and ArchitecturesNorthwestern Polytechnical UniversityXi’an 710072China Jieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory(Rongjiang Laboratory) Jieyang 515200China

Herein,the electromagnetic shielding performance of surface concave-convex(SC)and zig-zag micro-arrays was studied by using a simulation prediction and a three-dimensional(3D)printing custom ***,surface stripe concaveconvex(SSC)and surface cylindrical concave-convex(SCC)micro-arrays with or without zig-zag micro-arrays are designed,and their shielding performance is simulated in multi-bands(C-,X-band).The multiwalled carbon nanotubes/polydimethylsiloxane composites(MWCNT/PDMS)with different SC structures and different electrical conductivity are molded in acrylonitrile-butadiene-styrene copolymer(ABS)molds which are printed by a 3D *** results show that the electromagnetic interference shielding effectiveness(EMI SE)of the samples can be enhanced by constructing the SC micro-arrays with zig-zag micro-arrays,and improving with the increase of conductivity and *** addition,the shielding mechanism of the SC-MWCNT/PDMS composites is investigated and discussed by an electromagnetic simulation.

关键词： surface micro-arrays electrical properties electromagnetic interference(EMI)shielding three-dimensional(3D)printing

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辽宁省水田土壤硅素肥力状况的初步研究

土壤通报 2003年第6期34卷 543-547页

作者：张玉龙李军刘鸣达黄毅沈阳农业大学土地与环境学院辽宁沈阳110161

通过田间调查、田间试验和室内分析,研究了辽宁省不同类型水田土壤的硅素肥力状况,结果表明,醋酸-醋酸钠(pH4)缓冲液法测定的辽宁省水田土壤的有效硅含量不能准确反映土壤的供硅能力,淹水培养法的测定过程同水稻的生长过程相似,测定出... 详细信息

通过田间调查、田间试验和室内分析,研究了辽宁省不同类型水田土壤的硅素肥力状况,结果表明,醋酸-醋酸钠(pH4)缓冲液法测定的辽宁省水田土壤的有效硅含量不能准确反映土壤的供硅能力,淹水培养法的测定过程同水稻的生长过程相似,测定出的水浸态硅含量同硅肥的增产效果密切相关,因此,这种方法的测定结果可以用于评价水田土壤的供硅能力;田间试验结果表明,合理施用硅肥可提高水稻的产量,增产幅度和水浸态硅含量的关系可用CateandNelsen校验曲线进行描述;通过该曲线初步确定的辽宁省硅素丰缺的临界标准为水浸态硅含量80mg/kg;根据初步确定的临界标准,辽宁省绝大多数的水田土壤硅素缺乏,合理施用硅肥将成为辽宁省水稻产量提高的重要措施之一。

关键词：辽宁水田土壤硅素土壤肥力临界标准田间试验室内分析淹水培养法

系统性红斑狼疮患者中miR-410表达及其靶基因生物信息学分析

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中国免疫学杂志 2017年第6期33卷 884-888页

作者：张济欧大明黄丽芳欧阳琳邹礼衡南华大学附属第一医院风湿免疫科衡阳421001

目的:检测miR-410在系统性红斑狼疮患者(SLE)中的表达水平,运用生物信息学方法研究miR-410及其靶基因在SLE发生发展过程中的作用。方法:定量检测miR-410在SLE患者外周血单个核细胞中的表达水平,并通过miR-410序列分析,靶基因预测和Genec... 详细信息

目的:检测miR-410在系统性红斑狼疮患者(SLE)中的表达水平,运用生物信息学方法研究miR-410及其靶基因在SLE发生发展过程中的作用。方法:定量检测miR-410在SLE患者外周血单个核细胞中的表达水平,并通过miR-410序列分析,靶基因预测和Genecards数据库分析,进一步对其靶基因进行GO富集和KEGG Pathway分析。结果:miR-410在SLE患者中表达显著降低,其核苷酸序列在多物种间呈高度保守性。受miR-410调控且与SLE疾病相关的潜在靶基因包括FASLG、CSF2、IFNAR2、MAPK1、PLCG2、IL4等。GO分析发现miR-410的靶基因参与细胞生长增殖、程序性死亡、细胞分化、免疫系统发育等多个生物过程。KEGG Pathway分析发现miR-410的靶基因显著富集在肿瘤途径信号通路、细胞因子-细胞因子受体相互作用信号通路、胶质瘤信号通路、黑色素瘤信号通路、TGF-β和JAK-STAT信号通路等。结论:miR-410可能通过直接靶向作用其调控靶分子,影响SLE患者体内多条信号通路的网络调控,从而参与SLE疾病的发生和发展。

关键词： miR-410 SLE 靶基因 GO富集 KEGG信号通路

Success of intravenous immunoglobulin and steroids in managing severe COVID-19 following lung transplantation:A case report

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World Journal of Transplantation 2025年第1期15卷 178-184页

作者： Yen-Shou Kuo Kuan-Hsun Lin Ying-Yi Chen Yuan-ming Tsai Ti-Hui Wu Hsu-Kai huang Tsai-Wang huang Division of Thoracic Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical CenterTaipei 114202Taiwan

BACKGROUND Coronavirus disease 2019(COVID-19)pneumonia with severe septic shock and acute respiratory distress syndrome(ARDS)are critical illnesses for patients following *** immunoglobulin(IVIG)plays a role in both immune support and inflammation control,especially in immunocompromised *** case report describes the first successful experience using IVIG and pulse steroids to manage this critical condition following lung *** SUMMARY A 65-year-old male patient reported a history of chronic obstructive pulmonary disease and poor lung function and received bilateral sequential lung ***,he developed COVID-19 pneumonia,severe septic shock,and *** recovered from this critical condition after empirical antibiotics administration and veno-venous extracorporeal membrane oxygenation,in addition to IVIG and pulse *** IVIG is a valuable adjunct in managing severe sepsis in lung transplant recipients after COVID-19 *** aim,for the first time,to report the success of such a management approach for COVID-19 ARDS and sepsis in the post-lung transplant *** further investigations,this is a starting point for wider analysis of such an approach in this setting and consequently helps guide clinical practice for such a challenging patient population moving forward.

关键词： COVID-19 Septic shock Intravenous immunoglobulin Lung transplantation Post-transplant complications Case report

Enhancing postoperative pain control by surgically-initiated rectus sheath block in abdominal aortic aneurysm open repair: A case report

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World Journal of Clinical Cases 2025年第6期13卷 41-47页

作者： Kuan-Hua Chen ming-Yuan Kang Yi-Ting Chang Sheng-Yang huang Yung-Szu Wu Center for Cardiovascular Disease Taichung Veterans General HospitalTaichung 407219TaiwanChina Department of Anesthesiology Taichung Veterans General HospitalTaichung 407219TaiwanChina Division of Pediatric Surgery Department of SurgeryTaichung Veterans General HospitalTaichung 407219TaiwanChina School of Medicine National Yang Ming Chiao Tung UniversityTaipei 112304TaiwanChina Department of Post-Baccalaureate Medicine National Chung Hsing UniversityTaichung 402202TaiwanChina

BACKGROUND Abdominal aortic aneurysm(AAA)repair often involves significant postoperative pain,traditionally managed with systemic opioids,which can cause undesirable side *** case report explores the novel use of a surgically-initiated rectus sheath block with a catheter-over-needle assembly for pain management in AAA *** SUMMARY A 67-year-old female with hypertension and previous aortic dissection underwent elective open repair of an infrarenal AAA,which had grown from 3.4 cm to 4.3 cm over 14 months.A rectus sheath block was initiated surgically for postoperative pain *** patient reported low pain scores and did not require systemic intravenous opioids,enabling early ambulation and discharge on postoperative day seven without *** preventing complications of systemic opi-oids,the method indicating a promising direction for postoperative pain management in major vascular *** Surgically-initiated rectus sheath block as a valuable tool for managing postoperative pain in AAA repair.

关键词： Abdominal aortic aneurysm Postoperative pain management Rectus sheath block Surgical anesthesia Opioid-sparing techniques Case report

Role of nutrition in diabetes mellitus and infections

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World Journal of Clinical Cases 2025年第3期13卷 1-8页

作者： Xue-Lu Yu Li-Yun Zhou Xiao huang Xin-Yue Li ming-Ke Wang Ji-Shun Yang Naval Medical Center of PLA Naval Medical UniversityShanghai 200052China

In this editorial,we have commented on the article that has been published in the recent issue of World Journal of Clinical *** authors have described a case of unilateral thyroid cyst and have opined that the acute onset of infection may be linked to diabetes mellitus(DM).We have focused on the role of nutrition in the association between DM and *** with DM are at a high risk of infection,which could also be attributed to nutrition-related *** interventions for patients with diabetes are mainly based on a low-calorie diet,which can be achieved by adhering to a low-carbohydrate ***,dietary fiber supplementation is recommended to maintain the diversity of the gut ***,high-quality protein can prevent the increased risk of infection due to *** of vitamins C,vitamins A,vitamins D,and folic acid improves blood sugar control and facilitates immune *** deficiencies augment the risk of infection,but the relationship with diabetes is mostly U-shaped and a good intake should be maintained.

关键词： Diabetes mellitus Infection Macronutrients Vitamins Minerals

Experimental investigation on the failure mechanism of a rock landslide controlled by a steep-gentle discontinuity pair

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Journal of Mountain Science 2019年第6期16卷 1258-1274页

作者： huang da ZHONG Zhu GU Dong-ming State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400044China School of Civil and Transportation Engineering Hebei University of TechnologyTianjin 300401China

A type of rock landslide is very common in practical engineering, whose stability is mainly controlled by the rock bridge between the steep tensile crack at the crest and the low-inclination weak discontinuities at the toe(namely, ligament is the term for the locking section in the slope). To obtain a deeper understanding into the failure process of this kind of landslide, twenty-four physical slope models containing a steep-gentle discontinuity pair(a steep crack in the upper part and a low-inclination discontinuity in the lower part) were tested by applying vertical loads at the crests. The results indicate that the inclination angle of the ligament(θ) has great influence on the failure and stability of this type of rock slope. With the change of θ, three failure patterns(five subtypes) concerning the tested slopes can be observed, i.e., tensile failure of the ligament(Type 1), tension-shear failure of the ligament(Type 2) and two-stage failure of the main body(Type 3). The failure process of each failure mode presents five stages in terms of crack development, vertical load, horizontal/vertical displacements and strains in the ligaments. The specific range of the ligament angle between different failure patterns is summarized. The discussion on the failure resistances and ductility of different failure patterns, and the guiding significances of the experimental findings to the stability evaluation and the reinforcement were conducted.

关键词： Rock landslide Failure pattern Failure evolution Locking section Crack coalescence