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QTL analysis of flag leaf in barley (Hordeum vulgare L.) for morphological traits and chlorophyll content

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第12期9卷 938-943页

作者： da-wei xue Ming-can CHEN Mei-xue ZHOU Song CHEN Ying MAO Guo-ping ZHANG Agronomy Department Zhejiang University Hangzhou 310029 China College of Life and Environment Sciences Hangzhou Normal University Hangzhou 310036 China College of Agronomy Henan University of Science and Technology Luoyang 471003 China Tasmanian Institute of Agricultural Research and School of Agricultural Science University of Tasmania Kings Meadows TAS 7249 Australia

To understand genetic patterns of the morphological and physiological traits in flag leaf of barley, a double haploid （DH） population derived from the parents Yerong and Franklin was used to determine quantitative trait loci （QTL） controlling length, width, length/width, and chlorophyll content of flag leaves. A total of 9 QTLs showing significantly additive effect were detected in 8 intervals on 5 chromosomes. The variation of individual QTL ranged from 1.9% to 20.2%. For chlorophyll content expressed as SPAD value, 4 QTLs were identified on chromosomes 2H, 3H and 6H; for leaf length and width, 2 QTLs located on chromosomes 5H and 7H, and 2 QTLs located on chromosome 5H were detected; and for length/width, I QTL was detected on chromosome 7H. The identification of these QTLs associated with the properties of flag leaf is useful for barley improvement in breeding programs.

关键词： Barley (Hordeum vulgare L.) Double haploid (DH) Flag leaf Quantitative trait loci (QTL)

Optimization of Quenching Process for ZTAP/ High Chromium Cast Iron Composites

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Journal of Wuhan University of Technology(Materials Science) 2022年第6期37卷 1137-1142页

作者： WEI Hongming JIANG Yehua ZHANG Xiaozu JIA Yuanwei ZHOU Mojin xue da Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China

In order to solve the problem of cracking of ZTAP(Zirconia toughened alumina ceramic particles)reinforced HCCI(high chromium cast iron)matrix composites,the quenching process was *** reinforced HCCI matrix composites were prepared by infiltration method with gravity sand *** thermal expansion curves of HCCI and the composites were measured at different cooling rates by *** microstructure of the HCCI matrix and the composites were characterized by X-ray diffraction,light microscopy,SEM,ESD,and *** tested mechanical properties include Rockwell hardness and impact *** deformation differences of HCCI and the composite at different cooling rates were obtained according to the test results of thermal expansion coefficient curve and changes in microstructure and mechanical properties,and air cooling was the most favorable for the composites to have good hardness and not easy to *** cooling rate during air cooling is approximately equal to 21℃/s in this *** the quenching process was air cooling,the impact toughness and hardness of the composites are 3.7 J/cm^(2) and 61.8 HRC,respectively,and the deformation difference between the composites and HCCI was 20μm at 300℃.

关键词： composites ZTAP quenching cooling rate microstructure thermal expansion

Relationship between subgroups of central and lateral lymph nodemetastasis in clinically node-negative papillary thyroid carcinoma

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World Journal of Clinical Cases 2022年第12期10卷 3709-3719页

作者： Jing Zhou da-xue Li Han Gao Xin-Liang Su Department of Thyroid and Breast Surgery Chongqing Health Center for Women and ChildrenChongqing 401120China Department of Thyroid and Breast Surgery First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Lymph node metastasis(LNM) of papillary thyroid carcinoma(PTC) has a certain regularity and occurs first to the central lymph node and then to the lateral lymph node. The pathway of PTC LNM can guide surgical prophylactic lymph node dissection(LND) for clinical *** To investigate the relationship between subgroups of central LNM and lateral LNM in unilateral clinically node-negative PTC(cN0-PTC).METHODS data were collected for 1089 PTC patients who underwent surgical treatment at the Department of Endocrine and Breast Surgery of the First Hospital of Chongqing Medical University from January 2016 to December 2017. A total of 388 unilateral cN0-PTC patients met the inclusion criteria and were enrolled in this study. The clinical and pathological data for these 388 patients who underwent total thyroidectomy + central LND + lateral LND were retrospectively analyzed. The relationship between the central LNM and lateral LNM subgroups was *** The coincidence rate of cN0-PTC was only 30.0%.Optimal scaling regression analysis showed that sex(57.1% vs 42.9%, P = 0.026), primary tumor size(68.8% vs 31.2%, P = 0.008), tumor location(59.7% vs 40.3%, P = 0.007), extrathyroid extension(ETE)(50.6% vs 49.9%, P = 0.046), and prelaryngeal LNM(57.1% vs 42.9%, P = 0.004) were significantly associated with ipsilateral level-II LNM. Their importance levels were 0.122, 0.213, 0.172, 0.110, and 0.227, respectively. Primary tumor size(74.6% vs 30.2%, P = 0.016), pretracheal LNM(67.5% vs 32.5%, P < 0.001), and paratracheal LNM(71.4% vs 28.6%, P < 0.001) were significantly associated with ipsilateral level-Ⅲ LNM. Their importance levels were 0.120, 0.408, and 0.351, respectively. Primary tumor size(72.1% vs 27.9%, P = 0.003), ETE(70.4% vs 29.6%, P = 0.016), pretracheal LNM(68.3% vs 31.7%, P=0.001), and paratracheal LNM(80.8% vs 19.2%, P < 0.001) were significantly associated with ipsilateral level-IV LNM. Their importance levels were 0.164, 0.146, 0.216, a

关键词： Papillary thyroid carcinoma Lymph node metastasis Clinically node-negative Prophylactic lymph node dissection Prelaryngeal

Description of adsorption of hydrophobic organic compounds on sediment using multi-component adsorption model

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Journal of Environmental Sciences 2002年第2期14卷 195-203页

作者： QUAN Xie LIU Zhen yu xue da ming ZHAO Ya zhi YANG Feng lin School of Environmental Science and Technology Dalian University of TechnologyDalian 116012China.

A chemical sequential separation procedure for sediment has been developed for the adsorptive investigation of hydrophobic organic compounds(HOCs) including four fractions: carbonate, hydrous metallic oxide(ferric oxide, manganese oxide and alumina), clay and organic matter. Adsorption isotherms of these hydrophobic solute probes, such as hexachloroethane, lindane and 1,2,4,5 tetrachlorobenzene were measured for model sorbents, model and natural sediment, and the latter of which was pretreated with the simplified sequential separation method. The linear and Langmuir models are applied to correlate the experimental data of humic substance and other model sorbents respectively. Multi component Adsorptive Model (MCAM) was used to simulate adsorption isotherms of model and natural sediment. The results reveal that(1) the separation efficiencies of carbonate, organic matter, ferric oxide, manganese oxide and alumina are 98.1%, 72.5%, 82.6%, 93.5% and 83.3%, respectively; (2) except for removing metallic oxide, the external structure of sediment is not changed greatly after separation; (3) the MCAM correlates the data of adsorption isotherm rather well with the maximal relative deviations of 9.76%, 6.78% and 9.53% for hexachloroethane, lindane and 1,2,4,5 tetrachlorobenaze in model sediment, respectively. The MCAM can clearly give expression to the different adsorptive mechanisms for HOCs in organic and inorganic matter, though the experimental data in each component are not very accurate due to the sequential separation efficiency.

关键词： hydrophobic organic compounds sediment adsorption model

Genotypic and Environmental Variations of Arabinoxylan Content and Endoxylanase Activity in Barley Grains

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Journal of Integrative Agriculture 2013年第8期12卷 1489-1494页

作者： ZHANG Xiao-qin xue da-wei WU Fei-bo ZHANG Guo-ping Agronomy Department Zhejiang University College of Life and Environmental Sciences Hangzhou Normal University

Arabinoxylan （AX） content in barley grains is an important quality determinant when barley is used as raw material of malt or beer production. The cultivar and environmental variations of total arabinoxylan （TAX）, water extractable arabinoxylan （WEAX） and endoxylanase activity （EA） were investigated using eight barley cultivars growing at seven locations with diverse environmental conditions. The results showed that both barley cultivar and location significantly affected the TAX, WEAX and EA levels, but the variations of TAX content and EA were mainly attributed to cultivar, while the impact of location on WEAX content was greater than that of cultivar. Correlation analysis indicated that TAX was significantly correlated to WUAX.

关键词： arabinoxylan barley (Hordeum vulgare L.) cultivar endoxylanase environment

Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broilers in an experimental necrotic enteritis model

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Animal Nutrition 2017年第4期3卷 399-405页

作者： Guang-da xue Shu-Biao Wu Mingan Choct Robert A.Swick Animal Science School of Environmental and Rural SciencesUniversity of New England

Subclinical necrotic enteritis(NE) causes devastating economic losses in the broiler chicken industry,especially in birds raised free of in-feed antibiotics. Prebiotics are potential alternatives to in-feed antibiotics. Yeast cell wall extract(YCW) derived from Saccharomyces cerevisiae is a prebiotic with known immune modulating effects. This study examined the effects of YCW and antibiotics(AB) during subclinical NE on broiler growth performance, intestinal lesions, humoral immune response and gut microflora metabolites. The study employed a 2 × 3 factorial arrangement of treatments. Factors were:NE challenge(yes or no) and feed additive(control, AB, or YCW). Each treatment was replicated in 8 floor pens with 15 birds per pen. Challenged birds had higher feed conversion ratio(FCR) than unchallenged birds on d 35(P < 0.05). Dietary inclusion of AB decreased FCR regardless of challenge(P < 0.05) on d 24 and 35. Inclusion of YCW reduced serum interleukin-1(IL-1) concentration in NE challenged birds(P < 0.01) and increased immunoglobulin(Ig) G(P < 0.05) and Ig M(P < 0.05) levels compared to other dietary treatments regardless of challenge. Yeast cell wall extract increased formic acid concentration in cecal contents during challenge and increased butyric acid concentration in unchallenged birds on d *** study indicates YCW suppressed inflammatory response, promoted generation of immunoglobulin and increased short chain fatty acid production suggesting potential benefits to bird health.

关键词： Subclinical necrotic enteritis Yeast cell wall extract Immune response Short chain fatty acid Broiler

Effect of six-stitch pancreaticojejunostomy on pancreatic fistula:A propensity score-matched comparative cohort study

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 277-283页

作者： Zhao-Lin Zeng Yan Sun da xue Pi-Li Liu Wang-Ming Chen Lei Zhang Department of General Surgery the Second Affiliated Hospital of Harbin Medical UniversityHarbin 150086China

Background:Clinically relevant postoperative pancreatic fistula(CR-POPF)is the most common and severe complication after pancreaticoduodenectomy(PD).Despite the development of numerous anastomotic surgical techniques to minimize CR-POPF,more than 30%of patients who undergo PD develop ***,we propose a novel pancreaticojejunostomy(PJ)technique and evaluate its efficacy and safety compared to traditional ***:This retrospective study enrolled 164 consecutive patients who underwent PJ after PD between January 2012 and June *** them,78(47.6%)underwent traditional PJ and 86(52.4%)underwent sixstitch *** primary outcome was CR-POPF at 1-month follow-up defined according to the revised 2016 International Study Group on Pancreatic Fistula *** adjust for baseline differences and selection bias,patients were matched by propensity scores,which left 63 patients with traditional PJ and 63 with six-stitch ***:Compared to patients who underwent traditional PJ(mean age 56.2±9.4 years),patients who underwent six-stitch PJ(mean age 57.4±11.4 years)had a lower CR-POPF *** risk of CR-POPF among patients who underwent six-stitch PJ was decreased by 81.7%after adjustment for age,sex,body mass index,and disease severity compared to patients who underwent traditional ***,the surgery time was reduced from 29 min for traditional PJ to 15 min for six-stitch PJ(P<0.001).Adverse effects such as abdominal fluid collection,abdominal bleeding,and wound infection were similar between two ***:Six-stitch PJ may be an effective and efficient PJ technique for patients who undergo PD surgery.

关键词： Duct-to-mucosa Pancreaticoduodenectomy Pancreaticojejunostomy Clinically relevant postoperative pancreatic fistula

The role of supplemental glycine in establishing a subclinical necrotic enteritis challenge model in broiler chickens

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Animal Nutrition 2017年第3期3卷 266-270页

作者： Guang-da xue Shu-Biao Wu Mingan Choct Robert A.Swick Department of Animal Science School of Environmental and Rural Sciences University of New England

Subclinical necrotic enteritis(NE) is an economically important disease in the broiler industry. With the move towards removal of antibiotics from feeds, solutions to control subclinical NE are desperately being sought. Dietary glycine has been shown to promote proliferation of Clostridium perfringens(Cp) and may thus be useful to include in a NE challenge model. A study was conducted to evaluate the effect of increased dietary glycine levels on subclinical NE. A 2 × 2 × 2 factorial arrangement of treatments was carried out using day-old male Ross 308 chicks(n = 624) allocated to 48 floor pens with 8 treatments of 6 replicates with 11 birds per treatment. Factors were: Cp challenge(C-or C+), Eimeria spp. challenge(Eor E+), and dietary glycine in the grower diet(0 or 10 g/kg). Birds had higher FCR when challenged with Eimeria(P < 0.01) or Cp(P < 0.05) on d 24 or Cp on d 35 but FCR was lower when fed glycine on d 24(P < 0.01). Supplementation of glycine reduced feed intake on d 24 and increased weight gain on d 35(P < 0.05). A Cp × Eimeria × glycine interaction(P < 0.05) showed a higher jejunal lesion scores in birds challenged with a combination of Cp and glycine compared with those with Eimeria and glycine or the unchallenged birds. Lesion score interactions between Cp and glycine(P < 0.05) in the ileum and Cp and Eimeria in the duodenum(P < 0.05) and ileum(P < 0.05) illustrated higher lesion scores in birds challenged with Cp without Eimeria or glycine compared to those not challenged with Cp. This study suggests that using glycine can partially replace Eimeria in a subclinical NE challenge model in promoting the intestinal lesions but not impairing chicken performance.

关键词： Subclinical necrotic enteritis Glycine Lesion score Performance Broiler

Genetic Analysis and Gene-Mapping of Two Reduced-Culm-Number Mutants in Rice

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Journal of Integrative Plant Biology 2006年第3期48卷 341-347页

作者： Hua Jiang Long-Biao Guo da-Wei xue da-Li Zeng Guang-Heng Zhang Guo-Jun Dong Ming-Hong Gu Qian Qian State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China Agricultural College Yangzhou University Yangzhou 225006 China

In the present study, In order to systematically dissect the genetic mechanism of rice （Oryza satlva L.） tilling for the super rice ideotype and the model system of branching development, two ethyl methane suifonate-induced rice reduced-culm-number（rcn） mutants from the progeny of Nippobare （O. satlva ssp. japonica）, namely rcn8 and rcn9, were used. Their maximum tillers were both less than 4. in addition, rcn9 had another major feature of rust-spotted leaves. Allelic tests between these two mutants and seven other recessive few-tiller mutants revealed that they were previously unknown loci. Genetic analysis showed that the rcn traits were all controlled by a pair of different recessive genes, designated as RCN8and RCNg, respectively. Two F2 populations derived from crosses between the rcn8 or rcn9 mutants and 93-11 were constructed. Linkage analysis using two rcn F2 mapping populations with published simple sequence repeat markers demonstrated that the RCN8 and RCN9 genes were mapped on the long arm of chromosome 1 （119.6 cM） and the short arm of chromosome 6 （63.6 cM）, respectively. The results of the present study are beneficial to map-based cloning and functional analysis of the RCN8 and RCN9 genes.

关键词： gene-mapping reduced-culm-number rice simple sequence repeat marker.