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The expression of the new epididymal luminal protein of PDZ domain containing 1 is decreased in asthenozoospermia

Asian Journal of Andrology 2018年第2期20卷 154-159页

作者： A-Juan Liang Gui-Shuan wang Ping Ping Shuang-Gang Hu Yu Lin yi Ma Zheng-Zheng {2. Han-Shu wang Fei Sun International Peace Maternity and Child Health Hospital Shanghai Key Laboratory for Reproductive Medicine Institute of Embryo-Fetal Original Adult Disease School of Medicine Shanghai Jiao Tong University Shanghai 200030 China Shanghai key laboratory for Assisted Reproduction and Reproductive Genetics Center for Reproductive Medicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200135 China Medical School Institute of Reproductive Medicine Nantong University Nantong 226001 China

Spermatozoa are not mature until they transit the epididymis where they acquire motility and the ability to fertilize an egg through sequential modifications. The epididymis has three functional regions, caput, corpus, and cauda, and the luminal proteins of the epididymis play important roles in the above modifications. However, the proteins with differential enrichment between the caput and cauda are still largely unknown. To reveal the functions of the caput and cauda during sperm maturation, luminal proteins from caput and cauda of mice were analyzed by isobaric tag for relative and absolute quantitation （iTRAQ）. Overall, 12. differentially enriched proteins were found, of which 46 were caput enriched and 82.were cauda enriched. Bioinformatic analysis showed that lipid metabolism was active in the caput; while anion- and cation-binding activity and phosphorus and organophosphate metabolism were active in the cauda. A new epididymal luminal protein, the caput-enriched PDZ domain containing 1 （Pdzkl）, also named Na^＋/H^＋ exchange regulatory cofactor 3 （NHERF3）, which plays a critical role in cholesterol metabolism and carnitine transport, was found in the lipid metabolism. Western blotting and immunofluorescence analyses showed that Pdzkl was expressed in the epididymis but not in the testis, and localized at the middle piece of the sperm tail. Pdzkl protein level was also reduced in the spermatozoa in case of asthenozoospermic patients compared with that in normozoospermic men, suggesting that Pdzkl may participate in sperm maturation regulation and may be associated with male infertility. These results may provide new insights into the mechanisms of sperm maturation and male infertility.

关键词： asthenozoospermia epididymis fertility PDZ domain containing 1 sperm

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A Scaled Underwater Launch System Accomplished by Stress Wave Propagation Technique

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chinese Physics Letters 2011年第2期28卷 136-139页

作者： WEI Yan-Peng wang yi-Wei FANG Xin HUANG Chen-Guang {2. Zhu-Ping key laboratory of Hydrodynamics and Ocean Engineering Institute of MechanicsChinese Academy of SciencesBeijing 100190 The State key laboratory of Nonlinear Mechanics Institute of MechanicsChinese Academy of SciencesBeijing 100190

A scaled underwater launch system based on the stress wave theory and the slip Hopkinson pressure bar(SHPB)technique is developed to study the phenomenon of cavitations and other hydrodynamic features of high-speed submerged *** present system can achieve a transient acceleration in the water instead of long-time acceleration outside the *** projectile can obtain a maximum speed of 30m/s in about 2.0μs by the SHPB *** cavitation characteristics in the stage of acceleration and deceleration are captured by the high-speed camera.

关键词： acceleration cavitation system

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Bariatric surgery induces alterations in effective connectivity between the orbitofrontal cortex and limbic regions in obese patients

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Science China(Information sciences) 2020年第7期63卷 49-59页

作者： Shijun duan Gang JI Guanya LI Yang HU Wenchao ZHANG Jia wang Dardo TOMASI Nora D.VOLKOW Yongzhan NIE Guangbin CUI Gene-Jack wang yi ZHANG Department of Radiology Tangdu Hospital Fourth Military Medical University State key laboratory of Cancer Biology National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases Fourth Military Medical University Center for Brain Imaging School of Life Science and Technology Xidian University laboratory of Neuroimaging National Institute on Alcohol Abuse and Alcoholism

Obese subjects show enhanced brain responses in motivation and reward neurocircuitry encompassing sensory and somatic integration-interception, motivation-reward(striatal), emotion, and memory processes, which attenuate frontal region activation during food cues. Bariatric surgery(BS) is the only reliable treatment for morbid obesity. Unfortunately, it is unknown how BS affects neurocircuitry after weight loss. We aimed to examine effects of BS on the basal activity of brain areas involved in reward and motivation processing, emotion, memory, and gut-brain interaction. We combined resting-state fMRI with amplitude of low-frequency fluctuation(ALFF) and Granger causality analysis(GCA) to assess interactions between regions within the frontal-mesolimbic circuitry in 16 obese subjects(OB) and 2. normal-weight(NW) *** OB group was studied at baseline and 1 month post BS. Comparisons between OB and NW, and preand post BS showed significant differences in ALFF in areas involved in drive(caudate, orbitofrontal cortex(OFC)), arousal(thalamus), and conditioning/memory(amygdala, hippocampus)(P < 0.05, FDR correction). GCA revealed that in the OB group, the OFC had greater connectivity to limbic regions(amygdala,hippocampus, and medial thalamus) and the caudate. Post BS, the connectivity of the OFC to limbic regions decreased, whereas the connectivity from the amygdala and hippocampus to the caudate and thalamus was enhanced, particularly in subjects with lower body mass index(BMI). OFC activation in the OB group was associated with BMI prior to surgery, and changes in OFC post surgery were associated with alterations in BMI. Overall, the functional connectivity of the OFC was significantly decreased. As it is important for salience attribution and connected to limbic brain regions involved with emotional reactivity and conditioning after BS, its significant association with BMI changes indicates the contribution of OFC changes to the improved control of eating behavi

关键词： fMRI Granger causality analysis obesity bariatric surgery frontal-mesolimbic

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Enterohemorrhagic Escherichia coli O157:H7 — Xuzhou City, Jiangsu Province, China, 2.01–2.2.

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China CDC weekly 2023年第14期5卷 311-314页

作者： Wenwen Zhu Hui Guo Jingjing Xu Weiwei Wu Yanmin yi Jiao wang Ran {2. Jing Tong Yangguang Du Xuzhou Municipal Center for Disease Control and Prevention Xuzhou CityJiangsu ProvinceChina Xuzhou Municipal Tongshan District Center for Disease Control and Prevention Xuzhou CityJiangsu ProvinceChina State key laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Xuzhou Municipal Center for Public Health Inspection Xuzhou CityJiangsu ProvinceChina

Escherichia coli(***)that produce Shiga toxin or verocytotoxin is commonly known as Shiga toxinproducing ***(STEC)or verocytotoxin-producing ***(VTEC).Hemorrhagic enteritis(HC),caused by foodborne zoonotic pathogen enterohemorrhagic ***(EHEC)O157:H7,was first reported in the United States in 1982.and has since caused outbreaks of human infections in various countries such as Canada,Japan,and *** China,the pathogen was isolated from the feces of patients with diarrhea in Xuzhou City,Jiangsu Province in *** later caused an outbreak in Xuzhou and adjacent areas from 1999 to 2.00(1).Measures that included investigation of diarrhea patients,identification of the pathogen,and clearing epidemiological characteristics were implemented to control the spread of the ***,a surveillance program was reformulated in 2.01 in Tongshan District,Xuzhou City based on the national O157:H7 surveillance program and the reality of Jiangsu *** program included 18 towns for diarrhea patients and food surveillance,and 3 major animal farming towns for animal *** program has led to changes in the detection rates and virulence genes of the *** this study,we analyzed O157:H7 surveillance data from 2.01 to 2.2. to predict possible transmission risks.

关键词： O157:H7 Xuzhou Canada

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利拉鲁肽对棕榈酸诱导心肌细胞损伤的保护作用

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天津医科大学学报 2024年第5期30卷 410-414,444页

作者：张子钊段军滢高怡王凯张帆张跃李广平天津市心血管病离子与分子机能重点实验室天津医科大学第二医院心脏科天津心脏病学研究所天津300211

目的:探讨利拉鲁肽(lira)对棕榈酸(PA)所致H9C2.鼠心肌细胞脂毒性损伤的干预效果及其相关机制。方法:在不同浓度的PA(0、50、100、150、2.0、300μmol/L)和不同时间(6、12.2. h)的条件下分别刺激H9C2.鼠心肌细胞(n=3),建立心肌细胞... 详细信息

目的:探讨利拉鲁肽(lira)对棕榈酸(PA)所致H9C2.鼠心肌细胞脂毒性损伤的干预效果及其相关机制。方法:在不同浓度的PA(0、50、100、150、2.0、300μmol/L)和不同时间(6、12.2. h)的条件下分别刺激H9C2.鼠心肌细胞(n=3),建立心肌细胞的脂毒性损伤模型,使用CCK8检测确定使H9C2.胞脂毒性损伤较大、细胞存活数最多的刺激条件。将成功建立模型的H9C2.胞分为对照组(CON)、CON+Lira(1000 nmol/L)组、CON+PA(150μmol/L)组和PA(150μmol/L)+Lira(1000 nmol/L)组进行油红O染色(n=3),检测细胞内脂质含量,以明确PA(150μmol/L)对心肌细胞有无脂毒性。将H9C2.胞根据不同的PA浓度(0、50、100、150、2.0、300μmol/L)分为6个组(CON、PA1、PA2.PA3、PA4和PA5)(n=3),按照不同的Lira浓度分为6组:CON组、PA组(150μmol/L)、CON+Lira3(1000 nmol/L)组、PA(150μmol/L)+Lira1(100 nmol/L)组、PA(150μmol/L)+Lira2.500 nmol/L)组和PA(150μmol/L)+Lira3(1000 nmol/L)组(n=3),用蛋白印迹测定凋亡相关蛋白的表达量及其与PA、Lira浓度的关系。结果:使用CCK8检测并最终确定PA为150μmol/L、刺激12.h的条件下,H9C2.胞的脂毒性损伤较大、细胞存活数最多。与CON组相比,PA组(150μmol/L)的细胞活性明显下降、脂滴明显增加(t=34.53,P2.32.3.307,均P2.白表达明显减少(F=7.618,P2.白表达明显增加(F=7.104,P2.32.7.104,均P2.肌细胞12.h后可造成心肌细胞脂毒性损伤模型,lira可以改善甚至逆转PA诱导的H9C2.肌细胞脂毒性损伤,其机制可能与抑制炎症、抑制细胞凋亡有关。

关键词：利拉鲁肽 H9C2.胞脂毒性心肌保护凋亡蛋白

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Natural killer cells are activated and play a protective role against ZIKA virus infection in mice

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Science Bulletin 2017年第14期62卷 982-984页

作者： Xuefeng duan Shihua Li Gary Wong Dongfang wang Haoyu wang Jiao Lu Yuhai Bi Xuancheng Lu yi Shi Jinghua Yan Min Fang George Fu Gao CAS key laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences University of chinese academy of sciences institute of Health sciences Anhui University laboratory Animal Center Chinese Center for Disease Control and Prevention (China CDC) International College University of Chinese Academy of Sciences National institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (China CDC) Savaid Medical School University of Chinese Academy of Sciences Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of Sciences

Zika virus(ZIKV)is a mosquito-borne Flavivirus that was responsible for an epidemic in the Americas during 2.15–2.16[1].ZIKV infections in adults have been linked to Guillain-Barrésyndrome[2.3],as well as abnormalities such as abortion,stillbirth,and microcephaly in some cases[4].In addition,we have reported that ZIKV infect testicular stem-like peritubular

关键词：病毒感染细胞活性保护作用杀伤自然小鼠对齐

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A Critical Time-Window for the Selective Induction of Hippocampal Memory Consolidation by a Brief Episode of Slow-Wave Sleep

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Neuroscience Bulletin 2018年第6期34卷 1091-1099页

作者： yi Lu Zheng-Gang Zhu Qing-Qing Ma Yun-Ting Su Yong Han Xiaodong wang Shumin {2. Yan-Qin Yu Department of Neurobiology Institute of NeuroscienceNational Health Commission and Chinese Academy of Medical Sciences Key Laboratory of Medical NeurobiologyZhejiang University School of Medicine

Although extensively studied, the exact role of sleep in learning and memory is still not very clear. Sleep deprivation has been most frequently used to explore the effects of sleep on learning and memory, but the results from such studies are inevitably complicated by concurrent stress and distress. Furthermore, it is not clear whether there is a strict time-window between sleep and memory consolidation. In the present study we were able to induce time-locked slow-wave sleep(SWS) in mice by optogenetically stimulating GABAergic neurons in the parafacial zone(PZ), providing a direct approach to analyze the influences of SWS on learning and memory with precise time-windows. We found that SWS induced by light for 30 min immediately or 15 min after the training phase of the object-in-place task significantly prolonged the memory from 30 min to 6 h. However, induction of SWS 30 min after the training phase did not improve memory, suggesting a critical time-window between the induction of a brief episode of SWS and learning for memory *** of a gentle touch to the mice during light stimulation to prevent SWS induction also failed to improve memory, indicating the specific role of SWS,but not the activation of PZ GABAergic neurons itself, in memory consolidation. Similar influences of light-induced SWS on memory consolidation also occurred for Y-maze spatial memory and contextual fear memory, but not for cued fear memory. SWS induction immediately before the test phase had no effect on memory performance, indicating that SWS does not affect memory retrieval. Thus, by induction of a brief-episode SWS we have revealed a critical time window for the consolidation of hippocampusdependent memory.

关键词： Parafacial zone Slow-wave sleep Memory consolidation Hippocampus Optogenetics

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Identification of novel molecular markers of mastitis caused by Staphylococcus aureus using gene expression profiling in two consecutive generations of chinese Holstein dairy cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 155-171页

作者： Di wang Lei Liu Serafino M.A.Augustino Tao duan Thomas J.Hall David E.MacHugh Jinhuan Dou {2. Zhang Yachun wang {2.ng Yu key laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture&National Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China UCD School of Agriculture and Food Science University College DublinDublin D04 V1W8Ireland Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Department of Animal Nutrition and Feed Science College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China UCD Conway institute of Biomolecular and Biomedical Research University College DublinDublin D04 V1W8Ireland

Background: Mastitis in dairy cows caused by Staphylococcus aureus is a major problem hindering economic growth in dairy farms worldwide. It is difficult to prevent or eliminate due to its asymptomatic nature and long persistence of infection. Although transcriptomic responses of bovine mammary gland cells to pathogens that cause mastitis have been studied, the common responses of peripheral blood leukocytes to S. aureus infection across two consecutive generations of dairy cattle have not been ***: In the current study, RNA-Seq was used to profile the transcriptomes of peripheral blood leukocytes sampled from S. aureus-infected mothers and their S. aureus-infected daughters, and also healthy non-infected mothers and their healthy daughters. Differential gene expression was evaluated as follows: 1) S. aureus-infected cows versus healthy non-infected cows(S vs. H, which include all the mothers and daughters), 2. S. aureus-infected mothers versus healthy non-infected mothers(SM vs. HM), and 3) S. aureus-infected daughters versus healthy noninfected daughters(SMD vs. HMD).Results: Analysis of all identified expressed genes in the four groups(SM, SMD, HM, and HMD) showed that EPOR,IL9, IFNL3, CCL2., IL2. were exclusively expressed in both the HM and HMD groups, and that they were significantly(P 1.2. were detected in the three comparisons, respectively. DEGs with P 2.were used for functional enrichment analyses. For the S vs. H comparison, DEGs detected included CCL2., IL13 and MMP3, which are associated with the IL-17 signaling pathway. In the SM vs. HM and SMD vs. HMD comparisons, five(BLA-DQB, C1 R, C2. FCGR1 A, and KRT10) and six(BLA-DQB, C3 AR1, CFI, FCAR, FCGR3 A, and LOC10498484) genes, respectively, were involved in the S. aureus infection ***: Our stud

关键词： Dairy cow Disease resistance Mastitis Peripheral blood leukocyte Staphylococcus aureus Transcriptome Two generations

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金虎杂交斑三倍体、二倍体生长、发育比较及形态性状差异分析

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海洋与湖沼 2024年第5期55卷 1302-1311页

作者：邓远苏金枝李振通黎琳琳邱弈树丁小雨段鹏飞王心怡王林娜刘阳马文辉赵侠李胜忠田永胜海水养殖生物育种与可持续产出全国重点实验室中国水产科学研究院黄海水产研究所山东青岛266071 新疆农业大学生命科学学院新疆乌鲁木齐830052 中国水产科学研究院海南创新研究院海南三亚572000 莱州明波水产有限公司山东莱州261400

为了解金虎杂交斑三倍体的生长与形态特征,利用金虎杂交斑三倍体(JH-3N)和二倍体群体(JH-2.),通过对比养殖试验分析了两种不同倍性杂交石斑鱼的生长性能,同时采用多元统计分析方法对JH-3N、JH-2.及母本棕点石斑鱼(EF)和父本蓝身大斑石斑... 详细信息

为了解金虎杂交斑三倍体的生长与形态特征,利用金虎杂交斑三倍体(JH-3N)和二倍体群体(JH-2.),通过对比养殖试验分析了两种不同倍性杂交石斑鱼的生长性能,同时采用多元统计分析方法对JH-3N、JH-2.及母本棕点石斑鱼(EF)和父本蓝身大斑石斑鱼(ET)的形态特征进行统计分析。结果显示,经90 d对比养殖后,JH-3N的终末体质量较JH-2.提高9.59%。性腺组织学观察表明,JH-3N卵巢与同期JH-2.相比发育明显滞后。经卡方检验显示,JH-3N与JH-2.、父本和母本的7个可数性状都没有显著性差异。单因素方差分析显示,4个石斑鱼群体除M_(14)(臀鳍前端基部至尾鳍长)外,其他18个比例性状差异显著(P2.3%的3个主成分,差异主要体现在鱼体的背腹轴方向。判别分析显示,4个石斑鱼群体的判别准确率达100%,综合判别率为100%。研究结果表明,JH-3N具有生长速度更快、性腺发育滞后的优良性状,表型性状与JH-2.相比具有显著性差异,具备在生产上推广应用的前景,研究结果为金虎杂交斑倍性育种及种群鉴定提供了丰富的生物学基础数据。

关键词：金虎杂交斑三倍体生长速度形态特征多元统计

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金虎杂交斑三倍体血细胞特征及染色体核型分析

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海洋与湖沼 2024年第2期55卷 509-516页

作者：邓远李振通苏金枝黎琳琳邱弈树丁小雨段鹏飞王心怡王林娜刘阳马文辉赵侠李胜忠田永胜新疆农业大学生命科学学院新疆乌鲁木齐830052 农业农村部海洋渔业可持续发展重点实验室、中国水产科学研究院黄海水产研究所山东青岛266071 中国水产科学研究院海南创新研究院海南三亚572000 莱州明波水产有限公司山东莱州261400

为了解三倍体金虎杂交斑(棕点石斑鱼Epinephelus fuscoguttatus♀×蓝身大斑石斑鱼***♂)的细胞遗传特性。以静水压休克诱导的三倍体金虎杂交斑为研究对象,通过细胞DNA含量测定鉴定出三倍体金虎杂交斑、对三倍体金虎杂交斑进行红细胞大... 详细信息

为了解三倍体金虎杂交斑(棕点石斑鱼Epinephelus fuscoguttatus♀×蓝身大斑石斑鱼***♂)的细胞遗传特性。以静水压休克诱导的三倍体金虎杂交斑为研究对象,通过细胞DNA含量测定鉴定出三倍体金虎杂交斑、对三倍体金虎杂交斑进行红细胞大小、形态的比较及染色体核型分析。结果显示,三倍体金虎杂交斑的DNA含量与二倍体金虎杂交斑的DNA含量的比值为1.45︰1。三倍体金虎杂交斑血细胞长轴、短轴分别为二倍体的1.34、1.14倍(P2.07和1.54倍(P2.、1.05、1.2.、1.57倍(P2.=48=2.m+46t,NF=50,三倍体金虎杂交斑的染色体数目为72.核型为3n=72.1m+2.m+69t,NF=75,未发现有性染色体。实验结果为石斑鱼杂交种多倍体育种提供了丰富生物学基础数据。

关键词：金虎杂交斑红细胞形态流式细胞术三倍体二倍体染色体核型

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