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A reference-guided TILLING by amplicon-sequencing platform supports forward and reverse genetics in barley

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Plant Communications 2022年第4期3卷 37-53页

作者： Congcong Jiang Miaomiao Lei Yu Guo Guangqi Gao lijie Shi Yanlong Jin Yu Cai Axel Himmelbach Shenghui Zhou Qiang He Xuefeng Yao Jinhong Kan Georg Haberer Fengying duan Lihui Li Jun Liu Jing Zhang Manuel Spannagl Chunming Liu Nils Stein Zongyun Feng Martin Mascher Ping Yang Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijingChina College of Agronomy Sichuan Agricultural UniversityChengduChina Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) SeelandGermany Institute of Botany Chinese Academy of SciencesBeijingChina Plant Genome and Systems Biology(PGSB) Helmholtz Center MunichGerman Research Center for Environmental HealthNeuherbergGermany

Barley is a diploid species with a genome smaller than those of other members of the Triticeae tribe,making it an attractive model for genetic studies in Triticeae *** recent development of barley genomics has created a need for a high-throughput platform to identify genetically uniform mutants for gene function *** this study,we report an ethyl methanesulfonate(EMS)-mutagenized population consisting of 8525M_(3) lines in the barley landrace“Hatiexi”(HTX),which we complement with a high-quality de novo assembly of a reference genome for this *** mutation rate within the population ranged from 1.51 to 4.09 mutations per megabase,depending on the treatment dosage of EMS and the mutation discrimination platform used for genotype *** implemented a three-dimensional DNA pooling strategy combined with multiplexed amplicon sequencing to create a highly efficient and cost-effective TILLING(targeting induced locus lesion in genomes)platform in *** were successfully identified from 72 mixed amplicons within a DNA pool containing 64 individual mutants and from 56 mixed amplicons within a pool containing 144 *** discovered abundant allelic mutants for dozens of genes,including the barley Green Revolution contributor gene Brassinosteroid insensitive 1(BRI1).As a proof of concept,we rapidly determined the causal gene responsible for a chlorotic mutant by following the MutMap strategy,demonstrating the value of this resource to support forward and reverse genetic studies in barley.

关键词： barley mutagenesis TILLING amplicon-seq genetics

Comparing Immunity Triggered by flg22 Derived from Nonpathogenic Pseudomonas fragi Sneb1990 and Pathogenic P. syrinage pv. tomato DC3000

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Comparing Immunity Triggered by flg22 Derived from Nonpathog...

植物寄生线虫生物防治中存在的问题及解决途径

中国植物病理学会2024年学术年会

作者： ZHAO Shuangling ZHU Xiaofeng FAM Haiyan LIU Xiaoyu WANG Yuanyuan YANG Ning CHEN lijie duan Yuxi College of Plant Protection Shenyang Agricultural University Nematology Institute of Northern China Shenyang Agricultural University College of Sciences Shenyang Agricultural University College of Biological Science and Technology Shenyang Agricultural University

Tomato（Solanum lycopersicum L.） is one of the most economically valuable vegetables for both the fresh and the processed vegetable ***-parasiticnematodes disease,caused by root-knot nematodes（Meloidogyne spp.）,has become endemic in many green-house and caused tremendous economic losses to the vegetable *** have a multilayer immune system to defend themselves against pathogen ***,it is of vital importance to investigate the biocontrol bacteria that stimulate the plant immune system to help improve the resistance of tomato to root-knot nematode *** previous results showed that biocontrol bacteria Pseudomonas fragi Sneb1990 could trigger defense-related gene expression changes in tomato,and the amount of nematode infestation after treatment with flg22 was significantly less than that of *** this study,comparison of the defense response of tomato to the flg22 derived from *** Sneb1990 and pathogenicPst DC3000,was performed by analyzing the expression of several defense-associated *** root tissue from root dip treatment with flg22,the expression of canonical defense-related genes was *** data suggested that flg22 specifically induced the expression of certain defense-associated genes in Solanum lycopersicum L.L402 tomato as early as 6 h,and up to 24 h（PR2,NPR1 and PR4） after *** only gene that did not show a significant expression in the first 72 h was *** the case of lignin synthesis related genes expression level,we observed that higher expression levels of PAL（by 5.2-fold） after flg22 treatment compared to the positive controls flg22 and the water *** experiment results may reveal the mechanism by which tomato rapidly responds to the flg22-triggered response and modulates plant immunity to reduce root-knot nematode infestation.

关键词： Pseudomonas fragi Sneb 1990 flg22 plant immunity root-knot nematodes

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植物保护 2006年第4期32卷 4-9页

作者：杨宁段玉玺陈立杰沈阳农业大学植保学院植物线虫学研究室沈阳110161

根据国内外研究现状,针对在植物寄生线虫生物防治中存在的问题,提出了可行的解决办法———获得具有抗药性的线虫生防菌;同时,阐述了选择所兼容化学药剂的原则,客观地分析了线虫抗药性生防菌的意义及问题,并如何对其进一步研究、开发和... 详细信息

根据国内外研究现状,针对在植物寄生线虫生物防治中存在的问题,提出了可行的解决办法———获得具有抗药性的线虫生防菌;同时,阐述了选择所兼容化学药剂的原则,客观地分析了线虫抗药性生防菌的意义及问题,并如何对其进一步研究、开发和利用进行了展望。

关键词：线虫生物防治抗药性生防菌有害生物综合防治

我国能源消费结构变化趋势及对西部能源资源开发的影响

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中国矿业 2007年第1期16卷 14-16页

作者：段海峰王立杰中国矿业大学(北京)管理学院北京100083

日益严重的环境压力,迫使中国努力改善能源消费结构,而能源结构朝着清洁高效方向转化的客观实际,又必将给西部能源开发带来深刻影响:受生态、运输、市场、环境等多重制约,虽然西部煤炭资源丰富,但西部能源开发仍应以油气为开发重点;从... 详细信息

日益严重的环境压力,迫使中国努力改善能源消费结构,而能源结构朝着清洁高效方向转化的客观实际,又必将给西部能源开发带来深刻影响:受生态、运输、市场、环境等多重制约,虽然西部煤炭资源丰富,但西部能源开发仍应以油气为开发重点;从国家总体能源开发战略上看,油气资源的开发应该走“先海洋、后西部”的战略,将西部作为中国的能源战略储备基地;在开放西部油气市场上要审慎,在油气资源极其匮乏的条件下,保护资源应重于开发;“走出去”战略也切忌一哄而上;优先开发可再生能源,但要量力而行;另外,西部不仅要开发清洁能源,更应该成为中国使用清洁能源的典范。

关键词：西部能源消费结构资源开发

妊娠期新型冠状病毒肺炎的中医认识及辨证与治疗要点

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中医杂志 2020年第19期61卷 1666-1670页

作者：范丽洁杜惠兰段梅河北中医学院河北省石家庄市050200 河北省中西医结合生殖疾病协同创新中心

介绍中医对妊娠期新型冠状病毒肺炎的认识及证治特点。指出妊娠期阴亏肺燥、气滞气逆、湿郁痰停是其主要病因;正虚邪陷、疫毒阻肺、闭而不宣是主要病机。并以《妊娠期新型冠状病毒肺炎中医药治疗专家建议(试行)》为基础,结合《新型冠状... 详细信息

介绍中医对妊娠期新型冠状病毒肺炎的认识及证治特点。指出妊娠期阴亏肺燥、气滞气逆、湿郁痰停是其主要病因;正虚邪陷、疫毒阻肺、闭而不宣是主要病机。并以《妊娠期新型冠状病毒肺炎中医药治疗专家建议(试行)》为基础,结合《新型冠状病毒感染的肺炎诊疗方案(试行第七版)》对妊娠期新型冠状病毒肺炎的辨证与治疗要点进行解读和探析。强调治疗妊娠期新型冠状病毒肺炎时应治病与安胎并举,尽可能规避妊娠禁忌药物的使用,如病情需要应用,应严格掌握用药剂量及时间,确保母胎安全。

关键词：新型冠状病毒肺炎妊娠辨证论治禁忌药物

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辽宁省水稻品种种子携带干尖线虫的调查

植物保护 2008年第3期34卷 95-99页

作者：段旭光段玉玺王胜君陈立杰沈阳农业大学植物保护学院植物线虫学研究室沈阳110161

对辽宁省主要水稻品种的种子上携带水稻干尖线虫(Aphelenchoides besseyi)的群体数量进行了调查,结果表明沈稻3、辽粳294、旱禾、沈农389、1437、辽盐188、辽盐34等26个品种的种子上携带有水稻干尖线虫。胚乳发育正常的饱粒种子中带虫... 详细信息

对辽宁省主要水稻品种的种子上携带水稻干尖线虫(Aphelenchoides besseyi)的群体数量进行了调查,结果表明沈稻3、辽粳294、旱禾、沈农389、1437、辽盐188、辽盐34等26个品种的种子上携带有水稻干尖线虫。胚乳发育正常的饱粒种子中带虫率、线虫虫量最高,空粒种子带虫率最低。

关键词：水稻水稻干尖线虫种子带虫量

Genome-wide Identification of Gretchen Hagen 3 Genes in Glycine max:Identification,Structure Analysis and Response to Biotic and Abiotic Stress

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Genome-wide Identification of Gretchen Hagen 3 Genes in Glyc...

连作和轮作对大豆胞囊线虫群体数量及土壤线虫群落结构的影响

中国植物病理学会2023年学术年会

作者： YANG Xiaowen LIU Ting ZHU Xiaofeng FAN Haiyan LIU Xiaoyu XUAN Yuanhu WANG Yuanyuan YANG Ning CHEN lijie duan Yuxi College of Plant Protection Shenyang Agricultural University Nematology Institute of Northern China Shenyang Agricultural University College of Sciences Shenyang Agricultural University College of Biological Science and Technology Shenyang Agricultural University

Gretchen Hagen 3 genes conjugate excess of hormones with amino acids to maintain endogenous hormone *** patterns of GH3 genes have been characterized in many crops but not in *** this work, we identified members of GH3 family and analyzed the phylogenetic,gene duplication, structure, domain, conserved motifs and cis-elements in promoter region response to stresses.A comprehensive expression study under different stresses revealed that stress responsible GH3 genes clustered in Group-Ⅱ.Notably, five genes belong to Group-Ⅱ are involved in cold stress response,three genes in Group-Ⅱ-a are induced by dehydration but five in Group-Ⅱ-b are down, likes *** also observed the differential expression pattern of GH3 genes in soybean under pathogen infection, indicating Group-Ⅱ were induced by ***, we detected expression of Group-Ⅱ in soybean under soybean cyst nematode infection, suggested five genes in Group-Ⅱ-a are suppressed at 10 *** analysis presented here would help in the investigation of the function of GH3 genes in soybean during stress conditions.

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植物保护学报 2007年第4期34卷 347-352页

作者：陈立杰朱艳刘彬段玉玺沈阳农业大学植物保护学院线虫研究室沈阳110161

大豆胞囊线虫Heterodera glycinesIchinohe病害是大豆生产中的毁灭性病害,对世界大豆生产造成重大损失。采用根染色法和常规土壤线虫分类鉴定技术研究了连作和轮作两种耕作方式下大豆胞囊线虫群体数量和土壤线虫群落结构的变化。结果表... 详细信息

大豆胞囊线虫Heterodera glycinesIchinohe病害是大豆生产中的毁灭性病害,对世界大豆生产造成重大损失。采用根染色法和常规土壤线虫分类鉴定技术研究了连作和轮作两种耕作方式下大豆胞囊线虫群体数量和土壤线虫群落结构的变化。结果表明,连作使大豆田土壤中胞囊和根系上各龄期胞囊线虫数量显著增加(95.6→335.5),而轮作积累很少(10.3→31.8),感病品种中J2的侵入数量决定了大豆胞囊线虫形成胞囊的数量(22.8→95.6,129.4→335.5)。玉米-玉米-大豆的轮作方式使胞囊积累最少(10.3)。轮作大豆田土壤线虫总数高于连作,主要由于轮作大豆田土壤线虫的优势属Helicotylenchus和优势营养类群植物寄生线虫PP的相对丰度高于连作,但大豆胞囊线虫的相对丰度正相反,连作(33.65%)高于轮作(0.91%),说明Heterodera glycines可以作为土壤线虫的关键属种用以指示土壤发病的严重程度。研究中重要的优势属和关键属种cp值均为3,说明其繁殖和抗干扰能力比较强。研究证明土壤线虫优势营养类群、优势属种和关键属种可以有效地指示农业土壤健康状况和大豆田的大豆胞囊线虫病害的发生程度。

关键词：连作轮作大豆胞囊线虫土壤线虫群落结构

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饲料中有机磷农药残留的检测

饲料工业 2009年第9期30卷 36-38页

作者：段伟伟杨丽杰霍贵成东北农业大学乳品重点实验室黑龙江省哈尔滨市150030

为了更好地认识和评价饲料中的有机磷农药残留情况,从而保证畜产品的生产安全性,实验利用DB-1701毛细管色谱柱分析技术,建立了在不同形态饲料中的4种有机磷农药的残留检测方法。结果表明:饲料中的有机磷农药使用乙腈作为萃取剂,经过匀... 详细信息

为了更好地认识和评价饲料中的有机磷农药残留情况,从而保证畜产品的生产安全性,实验利用DB-1701毛细管色谱柱分析技术,建立了在不同形态饲料中的4种有机磷农药的残留检测方法。结果表明:饲料中的有机磷农药使用乙腈作为萃取剂,经过匀浆萃取或超声萃取,在0.45μm有机滤膜上抽滤净化,在配有FPD检测器的Agilent气象色谱仪上检测,获得了满意结果。该方法测定了当前应用比较广泛的4种有机磷农药,回收率为72%～94%,RSD为2.05%～8.96%,方法的最小检出量为0.01～0.05μg/g。

关键词：饲料有机磷农药检测