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Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses

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Journal of Integrative Agriculture 2024年第7期23卷 2391-2406页

作者： Qianqian Wang Jie Wang Lei Zhang Xiaoxiao duan lijie Zhu Youming Zhang Yan Li Fuxiao Liu College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China College of Veterinary Medicine Inner Mongolia Agricultural UniversityHuhhot 010018China Shandong New Hope Liuhe Group Co. Ltd.Qingdao 266100China Qingdao Center for Animal Disease Control&Prevention Qingdao 266199China State Key Laboratory of Microbial Technology Shandong UniversityQingdao 266237China

Senecavirus A(SVA)has a positive-sense,single-stranded RNA *** 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)that have been demonstrated to be well ***,it is still unclear whether each stem-loop subdomain,such as a single stem or loop,is also highly *** clarify this issue in the present study,a set of 29 SVA cDNA clones were constructed by site-directed mutagenesis(SDM)on the *** SDM-modified scenarios included:(1)stem-formed complementary sequences exchanging with each other;(2)loop transversion;(3)loop transition;and(4)point *** cDNA clones were separately transfected into cells for rescuing viable viruses,whereas only four SVAs of interest could be recovered,and were genetically stable during 20 *** progeny grew significantly slower than the other three *** dual-luciferase reporter assay showed that none of the SDM-modified IRESes significantly inhibited the IRES *** previous study indicated that a single motif from any of the ten stem structures,if completely mutated,would cause the failure of virus ***,our present study revealed three stem structures,whose individual complementary sequences could exchange with each other to rescue sequence-modifying ***,one apical loop was demonstrated to have the ability to tolerate its own full-length transition,also having no impact on the recovery of sequence-modifying *** present study suggested that not every stem-loop structure was strictly conserved in its conformation,while the full-length IRES itself was well *** provides a new research direction on interaction between the IRES and many factors.

关键词： SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

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谷子米粒吸水率离心沥水测定方法研究

山西农业大学学报（自然科学版） 2014年第4期34卷 315-319页

作者：张义茹段黎杰陆洁侯蕊马芳芳李萍李红英山西农业大学农学院山西太谷030801 农业部黄土高原作物基因资源与种质创制重点实验室山西太原030031 新绛县农业委员会山西新绛043100

连续称重法测定种子或米粒的吸水率,一般采用滤纸吸除种子或米粒表面游离水。谷子米粒由于颗粒小,采用滤纸法容易粘米并损坏米粒,测定速度慢,效率低。本研究采用离心方法,将离心管底部打孔,设置不同转速梯度离心3min。以晋谷21为材料,... 详细信息

连续称重法测定种子或米粒的吸水率,一般采用滤纸吸除种子或米粒表面游离水。谷子米粒由于颗粒小,采用滤纸法容易粘米并损坏米粒,测定速度慢,效率低。本研究采用离心方法,将离心管底部打孔,设置不同转速梯度离心3min。以晋谷21为材料,设定米粒饱和吸水、不饱和吸水实验。研究确定了谷子米粒吸水率离心沥水测定方法的最佳方案为300rpm离心3min。

关键词：米粒吸水率离心沥水

基于InVEST模型的吉林省生态系统服务评估研究

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绿色科技 2021年第24期23卷 130-133,142页

作者：孟彩红王媛庄雨适苏伟李昂段丽杰孙同博徐国梅吉林环境科学研究院吉林长春130012

生态系统服务功能准确评估,是实现资源健康、可持续发展,更好地发挥生态服务功能的重要保障。InVEST模型具有定量评价准确率高、评价过程和结果空间表达清晰、情景模拟趋势预测功能强等特点,已广泛应用于生态系统服务功能评价的许多方... 详细信息

生态系统服务功能准确评估,是实现资源健康、可持续发展,更好地发挥生态服务功能的重要保障。InVEST模型具有定量评价准确率高、评价过程和结果空间表达清晰、情景模拟趋势预测功能强等特点,已广泛应用于生态系统服务功能评价的许多方面。概述了InVEST模型的生态系统服务功能评估的获取以及方法,系统评述了在应用InVEST模型对研究区生态系统的碳储量、水源涵养、生境质量、防风固沙、土壤保持等服务功能进行评估方面取得的研究进展,总结了各类应用中的启示,提出了利用InVEST模型开展生态系统服务功能评估的研究方向。

关键词：生态系统服务功能遥感 InVEST模型吉林省

Trace Element and Sulfur Isotope Compositions of Gold-bearing Pyrite from the Zhangjiawa Iron Skarn Deposit,Laiwu District:Implications for Sources of Early Cretaceous Gold Mineralization in the North China Craton

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Acta Geologica Sinica(English Edition) 2021年第5期95卷 1771-1774页

作者： duan Zhuang FU Yangang LI Chunjia ZHAO Shaorui GAO Mingbo GAO Jilei JIN lijie LI Siyuan NGO Dac Xuan Institute of Geophysical and Geochemical Exploration Chinese Academy of Geological SciencesLangfangHebei 065000China Key Laboratory for Geochemical Exploration Technology Ministry of Natural ResourcesLangfangHebei 065000China No.1 Institute of Geology and Mineral Resources of Shandong Province Jinan 250100China Shandong Engineering Laboratory for High-Grade Iron Ore Exploration and Exploitation Jinan 250100China School of Earth Resources China University of GeosciencesWuhan 430074China Department of Mineral Resource Prospecting and Exploration Hanoi University of Mining and GeologyHanoi 100000Viet Nama

Objective The Zhangjiawa iron deposit is the largest-tonnage and representative iron skarn deposit,with proven reserves of 290 Mt at an average of 46%Fe(up to>65%),in the Laiwu district,eastern North China Craton(NCC)(Figs.1 a,b;duan and Li,2017).Iron mineralization at Zhangjiawa is mainly hosted along a northern contact zone between the Kuangshan dioritic intrusion and Middle Ordovician carbonate rocks that host numerous evaporite intercalations(Figs.1b,c).

关键词： Cretaceous Ordovician carbonate

Epidemic Characteristics and Spatio-Temporal Patterns of HFRS in Qingdao City,China,2010-2022

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Biomedical and Environmental Sciences 2024年第9期37卷 1015-1029页

作者： Ying Li Runze Lu Liyan Dong Litao Sun Zongyi Zhang Yating Zhao Qing duan lijie Zhang Fachun Jiang Jing Jia Huilai Ma Chinese Field Epidemiology Training Program Chinese Center for Disease Control and PreventionBeijing 100050China National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Qingdao Municipal Center for Disease Control and Prevention Qingdao 266024ShandongChina Jiaozhou Center for Disease Control and Prevention Qingdao 266399ShandongChina Institute for Infectious Disease Control and Prevention Shandong Center for Disease Control and PreventionJinan 250013ShandongChina Office of Epidemiology(Technical Guidance Office for Patriotic Health Work) Chinese Center for Disease Control and PreventionBeijing 102206China

Objective This study investigated the epidemic characteristics and spatio-temporal dynamics of hemorrhagic fever with renal syndrome(HFRS)in Qingdao City,*** Information was collected on HFRS cases in Qingdao City from 2010 to *** epidemiologic,seasonal decomposition,spatial autocorrelation,and spatio-temporal cluster analyses were *** A total of 2,220 patients with HFRS were reported over the study period,with an average annual incidence of 1.89/100,000 and a case fatality rate of 2.52%.The male:female ratio was 2.8:1.75.3%of patients were aged between 16 and 60 years old,75.3%of patients were farmers,and 11.6%had both“three red”and“three pain”*** HFRS epidemic showed two-peak seasonality:the primary fall-winter peak and the minor spring *** HFRS epidemic presented highly spatially heterogeneous,street/township-level hot spots that were mostly distributed in Huangdao,Pingdu,and *** spatio-temporal cluster analysis revealed three cluster areas in Qingdao City that were located in the south of Huangdao District during the fall-winter *** The distribution of HFRS in Qingdao exhibited periodic,seasonal,and regional characteristics,with high spatial clustering *** typical symptoms of“three red”and“three pain”in patients with HFRS were not obvious.

关键词： Hemorrhagic fever with renal syndrome Epidemic characteristics Spatio-temporal distribution

1961-2006年湖南省霾现象的变化特征

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气候变化研究进展 2007年第5期3卷 260-265页

作者：廖玉芳吴贤云潘志祥赵福华祝燕德段丽洁李超李艳崔巍李秋林湖南省气候中心长沙410007 湖南省气象局长沙410007

利用1961-2006年湖南省97个市(县)地面气象观测资料,分析了霾现象的变化特征,结果表明:20世纪70年代以来,湖南年霾日急剧增多,极值不断被刷新,强度增强,重度霾出现范围增大。统计分析表明:46a来湖南年平均风速线性减小,年降水日数波动... 详细信息

利用1961-2006年湖南省97个市(县)地面气象观测资料,分析了霾现象的变化特征,结果表明:20世纪70年代以来,湖南年霾日急剧增多,极值不断被刷新,强度增强,重度霾出现范围增大。统计分析表明:46a来湖南年平均风速线性减小,年降水日数波动性减少,年平均日最小相对湿度≤70%的日数增多。气候变化、城市化进程加快、大气污染物排放量增加是引起霾出现频率增加的可能原因。

关键词：霾气候变化污染城市化

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p53 upregulated by HIF-la promotes hypoxiainduced G2/M arrest and renal fibrosis in vitro and in vivo

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Journal of Molecular Cell Biology 2019年第5期11卷 371-382页

作者： Limin Liu Peng Zhang Ming Bai lijie He Lei Zhang Ting Liu Zhen Yang Menglu duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun Department of Nephrology Xijing Hospital Fourth Military Medical University Xi'an 710032 China State Key Laboratory of Cancer Biology Fourth Military Medical University Xi'an 710032 China Department of Radiation Oncology Navy General Hospital Beijing 100048 China Department of Medicine Mayo Clinic College of Medicine and Mayo Graduate School Rochester MN 55905 USA

Hypoxia plays an important role in the genesis and progression of renal *** underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis.

关键词： renal tubulointerstitial fibrosis hypoxia cell cycle (G2/M) arrest p53 HIF-lα TGF-β

International standard of Traditional Chinese Medicine techniques:clinical guidelines for dysmenorrhea(2022)

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Journal of Traditional Chinese Medicine 2024年第6期44卷 1277-1287页

作者： LIANG Xiao ZHANG Li CHEN Junlu DU Yuhan JIANG Min FAN lijie MA Huirong duan Yancang SONG Xuping WANG Xiaohui DU Huilan Institute of Integrated Traditional Chinese and Western Medicine Hebei University of Chinese MedicineShijiazhuang 050091China College of Integrated Traditional Chinese and Western Medicine Hebei University of Chinese MedicineShijiazhuang 050091China Shanxi University of Chinese Medicine Xianyang 712046China Collaborative Innovation Center of Integrated Chinese and Western Medicine on Reproductive Disease Shijiazhuang 050091China Hebei Key Laboratory of Integrative Medicine on Liver-kidney Patterns Shijiazhuang 050091China School of Public Health Lanzhou UniversityLanzhou 730000China

Dysmenorrhea is a common gynecological condition that is further divided into two categories, namely primary and secondary dysmenorrhea. Traditional Chinese Medicine(TCM) gives good clinical results for the treatment of dysmenorrhea based on the basic principles of holistic view and syndrome differentiation. However, presently, the clinical practice guidelines in TCM for dysmenorrhea treatment are required. The guideline is formulated in strict accordance with the principles of evidence-based medicine with reference to the General Principles for the Compilation of International Guidelines for Diagnosis and Treatment of Traditional Chinese Medicine Syndromes and General Principles for the Compilation of International Clinical Practice Guidelines for Traditional Chinese Medicine Diseases and accordance with the principles and methods of The Appraisal of Guidelines for Research and Evaluation in China(AGREE-China) and World Health Organization guidelines. This guideline includes clinical issues by literature search and questionnaire survey, and eight background questions and 23 prospect questions were finally created. Based on the evidence retrieval, synthesis, consideration of patient preferences and values, intervention costs, advantages, and disadvantages, recommendations are created using the Delphi method and expert consensus method. This guideline clarified the definition, diagnosis, and TCM syndrome classification for dysmenorrhea, and recommends different TCM treatments based on the level of evidence, including Chinese herbal medicine, Chinese patent medicine, acupuncture, and other auxiliary therapies. The present guideline is an international clinical practice guideline for the treatment of dysmenorrhea using TCM. This guideline will provide substantive evidence and standardized guidance for the treatment of dysmenorrhea using TCM, improve the quality and safety of medical services, and standardize diagnosis and treatment plans for dysmenorrhea.

关键词： medicine Chinese traditional endometriosis adenomyosis dysmenorrhea practice guideline

Responses of Nematode Communities to Inorganic Fertilizer Disturbance in a Farmland Ecosystem

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Pedosphere 2002年第3期12卷 193-200页

作者： LIANGWENJU CHENlijie 等 InstituteofAppliedEcology theChineseAcademyofSciencesChenyang110016（China） LaboratoryofPlantNematology ShenyangAgriculturalUniversityShenyang110161（China）

Nematode communities were monitored in a field study at depths of 0～10 cmand 10～20 cm throughout a corn (Zea mays L.) growing season in the black soil region of NortheastChina. Plots were cropped corn with (IF) and without (NF) nitrogen fertilization. Fifteen familiesand 16 genera were observed in the nematode suspensions. Cephalobidae, Rhabditidae, Aphelenchus,Filenchus, and Helicotylenchus were found to be dominant genera/families in both plots at bothdepths. Significant differences were found between treatments and corn growing stages in the totalnumbers of nematodes during the study period. The total numbers of nematodes at the 0～10 cm depthwere higher in the inorganic fertilizer plot than in the control plot (no fertilizer applications)across jointing, filling, and mature stages. Bacterivores were the most abundant trophic groupsunder two treatments and at both depths, and the relative abundance averaged 42.8%.Omuivores-predators at both depths were lower in the inorganic fertilizer plot than in the controlplot during the study period, excluding corn jointing stage. Maturity index (MI) and plant parasiteindex (PPI) were used to detect the differences between the two treatments, MI and PPI wereeffective in distinguishing differences in nematode communities during corn growing season. The roleof nematode communities as bioindicators of ecosystem disturbance due to fertilization is noted.

关键词： bioindicator disturbance inorganic fertilizer maturity index nematodecommunity