检索结果-南通市图书馆

A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2024年第2期9卷 209-216页

作者： Yangyang li Cen li Yishan Fu Quan Zhang Jianing Ma Jingwen Zhou Jianghua li Guocheng du Song liu National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing Jiangnan UniversityWuxi214122China Science Center for Future Foods Jiangnan UniversityWuxi214122China School of Biotechnology Jiangnan University1800 Lihu RoadWuxi214122China Jiangsu Provisional Research Center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Life Sciences/Institute of Agro-bioengineeringGuizhou UniversityGuiyang550025China Dalian Research Institute of Petroleum and Petrochemicals SINOPECDalian116000China School of Chemical Engineering Dalian University of TechnologyDalian116000China

Aspergillus niger is a highly versatile fungal strain utilized in industrial *** expression levels of recombinant genes in *** can be enhanced by increasing the copy ***,given the prolonged gene editing cycle of ***,a“one-step”strategy facilitating the simultaneous integration of recombinant genes into multiple genomic loci would provide a definitive *** our previous study,a visual multigene editing system(VMS)was designed to knock out five genes,employing a tRNA-sgRNA array that includes the pigment gene albA and the target *** upon this system,hybrid donor DNAs(dDNAs)were introduced to establish a clustered regularly interspaced short palindromic repeats(CRISPR)-based multiplex integration ***,a CRISPR-Cas9 homology-directed repair(CRISPR-HDR)system was constructed in *** by co-transforming the CRISPR-Cas9 plasmid(with a highly efficient sgRNA)and the dDNA,resulting in precise integration of recombinant xylanase gene xynA into the target loci(theβ-glucosidase gene bgl,the amylase gene amyA,and the acid amylase gene ammA).Subsequently,the length of homology arms in the dDNA was optimized to achieve 100%editing efficiency at each of the three gene *** achieve efficient multiplex integration in ***,the CRISPR plasmid pLM2 carrying a sgRNA-tRNA array was employed for concurrent double-strand breaks at multiple loci(bgl,amyA,ammA,and albA).Hybrid dDNAs were then employed for repair,including dDNA1-3(containing xynA expression cassettes without selection markers)and dDNAalbA(for albA knockout).Among the obtained white colonies(RLM2′),23.5%exhibited concurrent replacement of the bgl,amyA,and ammA genes with xynA(three copies).Notably,the xynA activity obtained by simultaneous insertion into three loci was 48.6%higher compared to that obtained by insertion into only the bgl ***,this multiple integration toolkit successfully enhanced the expression of endogenous pectinase pelA and

关键词： Multiplex integration Aspergillus niger CRISPR-Cas9 homologous direct repair White colony

Iterative Learning Control for a Class of linear Continuous-time Switched Systems with Fixed Initial Shifts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Communications in Mathematical Research 2018年第4期34卷 309-328页

作者： xu guang-zhao fu qin du li-li wu jian-rong yu peng-fei li yong School of Mathematics and Physics Suzhou University of Science and TechnologySuzhouJiangsu215009 不详

This paper deals with the problem of iterative learning control for a class of linear continuous-time switched systems in the presence of a fixed initial shift. Here, the considered switched systems are operated during a finite time interval repetitively. According to the characteristics of the systems, a PD-type learning scheme is proposed for such switched systems with arbitrary switching rules, and the corresponding output limiting trajectories under the action of the PD-type learning scheme are given. Based on the contraction mapping method, it is shown that this scheme can guarantee the outputs of the systems converge uniformly to the output limiting trajectories of the systems over the whole time interval. Furthermore, the initial rectifying strategies are applied to the systems for eliminating the effect of the fixed initial shift. When the learning scheme is applied to the systems, the outputs of the systems can converge to the desired reference trajectories over a pre-specified interval. Finally, simulation examples illustrate the effectiveness of the proposed method.

关键词： iterative learning control switched system PD-type learning scheme fixed initial shift output limiting trajectory

Prescreening of large-effect markers with multiple strategies improves the accuracy of genomic prediction

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第5期23卷 1634-1643页

作者： Keanning li Bingxing An Mang liang Tianpeng Chang Tianyu Deng lili du Sheng Cao Yueying du Hongyan li lingyang Xu Lupei Zhang Xue Gao Junya li Huijiang Gao Laboratory of Molecular Biology and Bovine Breeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China College of Computer and Information Engineering Tianjin Agricultural UniversityTianjin 300384China College of Animal Science and Technology Qingdao Agricultural UniversityQingdao 266109China Tongliao Animal Agriculture Development Service Center Tongliao 028000China

Presently,integrating multi-omics information into a prediction model has become a ameliorate strategy for genomic selection to improve genomic prediction ***,we set the genomic and transcriptomic data as the training population data,using BSLMM,TWAS,and eQTL mapping to prescreen features according to |β_(b)|>0,top 1%of phenotypic variation explained(PVE),expression-associated single nucleotide polymorphisms(eSNPs),and egenes(false discovery rate(FDR)lidation population,*** results suggested that both GBLUP-Fix and GFBLUP models could improve the accuracy of longissimus dorsi muscle(LDM),water holding capacity(WHC),shear force(SF),and pH in Huaxi cattle on average from 2.14 to 8.69%,especially the improvement of GFBLUP-TWAS over GBLUP was 13.66%for *** methods also captured more genetic variance than *** study confirmed that multi-omics-assisted large-effects loci prescreening could improve the accuracyofgenomic prediction.

关键词： multi-omics data features prescreening eQTL mapping Huaxi cattle genomic selection

Synergy effect of regulated li-plating and functional solid electrolyte interphase on graphite anodes

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第9期17卷 8077-8085页

作者： Panpan Wang Baojia Xia Jianling li State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 201800China

The growth of li dendrites poses potential safety hazard to lithium-ion batteries(liBs),and eliminating li dendrites thoroughly stills face tough difficulties ***,regulating li-plating is a critical optimization-direction to address the ***,a“graphite-li hybrid”anode with high reversibility is realized under the constant-capacity lithiation(CCL).Within CCL,the uniform distribution of li-plating on the graphite surface is successfully *** evolution in different states of solid electrolyte interphase(SEI)is investigated in detail to study the interaction between the potentials and impedance during the process of liintercalation and *** the potential below 0 V and the state of charge(SOC)of 110%relative to the theoretical capacity,the F-rich SEI with high stability is constructed to hinder the emergency of li dendrites and maintain the intact structure of graphite anode under long *** cell presents more than 100%Coulombic efficiency(CE)with the 900 cycles,demonstrating the reversible li-plating and the utilization of *** the CCL half-cell provides a good cycling performance and specific capacity of 900 cycles at 0.5 C,it is attributed to the synergy effect of stable inorganic-rich SEI and regulated active li-plating.

关键词： "graphite-li hybrid"anode li-plating reversibility solid electrolyte interphase(SEI)

Insights into effects of thermotolerant nitrifying and sulfur-oxidizing inoculants on nitrogen-sulfur co-metabolism in sewage sludge composting

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第10期144卷 76-86页

作者： Tingting Hou Yujie Zhou Rongchun du Jiali liu Weiguang li Shumei Zhang Muzi li Junhong Chu liqiang Meng Institute of Microbiology Heilongjiang Academy of SciencesHarbin 150010China Hebei University of Environmental Engineering Hebei Key Laboratory of Agroecological SafetyQinhuangdao 066102China School of Environment Harbin Institute of TechnologyHarbin 150090China

In this study,high temperature thermotolerant nitrifying bacteria(TNB)and high temperature thermotolerant sulfide oxidizing bacteria(TSOB)were obtained from compost samples and inoculated into sewage sludge(SS)*** effects of inoculation on physical and chemical parameters,ammonia and hydrogen sulfide release,nitrogen form and sulfur compound content change and physical-chemical properties during nitrogen and sulfur conversion were *** results showed that inoculation of TNB and TSOB increased the temperature,pH,OM degradation,C/N ratio and germination index(GI)of *** with the control treatment(CK),the addition of inoculants reduced the release of NH3 and H2S,and transformed them into nitrogen and sulfur compounds,the hydrolysis of polymeric ferrous sulfate was promoted,resulting in relatively high content of sulfite and *** the same time,the physical and chemical properties of SS have a strong correlation with nitrogen and sulfur compounds.

关键词： Sewage sludge Composting Inoculation Thermotolerant nitrifying bacteria Thermotolerant sulfurized oxidizing bacteria

Considerations of advanced aqueous batteries recycling:A perspective

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第6期93卷 46-54,I0002页

作者： Yun Zhao Yuqiong Kang Hao du Feiyu Kang Baohua li Institute of Materials Research Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055GuangdongChina

*** Battery technology,serving as a basis for a rechargeable society,has developed rapidly over a century[1].The progress can be roughly divided into three stages,with the initial two mainly focusing on aqueous battery *** to 1859,research primarily focused on the conceptualization of batteries and the development of primary(non-rechargeable)cells[2].In the subsequent150 years,rechargeable battery technology emerged。

关键词： battery Battery primarily

Enhancing thermodynamic stability of single-crystal Ni-rich cathode material via a synergistic dual-substitution strategy

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第1期88卷 428-436,I0010页

作者： Jixue Shen Hui li Haoyu Qi Zhan lin Zeheng li Chuanbo Zheng Weitong du Hao Chen Shanqing Zhang School of Metallurgy Engineering Jiangsu University of Science and TechnologyZhangjiagang 215600JiangsuChina School of Metallurgy and Environment Central South UniversityChangsha 410083HunanChina Guangdong Laboratory of Chemistry and Fine Chemical Industry Jieyang Center Jieyang 515200GuangdongChina Institute of Sustainable Transformation School of Chemical Engineering and Light IndustryGuangdong University of TechnologyGuangzhou 510006GuangdongChina Zhejiang Provincial Key Laboratory of Advanced Chemical Engineering Manufacture Technology College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027ZhejiangChina Centre for Catalysis and Clean Energy School of Environment and ScienceGold Coast CampusGriffith UniversityGold Coast 4222QueenslandAustralia

Nickel(Ni)-rich cathode materials have become promising candidates for the next-generation electrical vehicles due to their high specific ***,the poor thermodynamic stability(including cyclic performance and safety performance or thermal stability)will restrain their wide commercial ***,a single-crystal Ni-rich li Ni_(0.83)Co_(0.12)Mn_(0.05)O_(2) cathode material is synthesized and modified by a dual-substitution strategy in which the high-valence doping element improves the structural stability by forming strong metal–oxygen binding forces,while the low-valence doping element eliminates high li^(+)/Ni^(2+)*** a result,this synergistic dual substitution can effectively suppress H2-H3 phase transition and generation of microcracks,thereby ultimately improving the thermodynamic stability of Ni-rich cathode ***,the dual-doped Ni-rich cathode delivers an extremely high capacity retention of 81%after 250 cycles(***/li+)in coin-type half cells and 87%after 1000 cycles(***/li^(+))in pouch-type full cells at a high temperature of 55℃.More impressively,the dual-doped sample exhibits excellent thermal stability,which demonstrates a higher thermal runaway temperature and a lower calorific *** synergetic effects of this dual-substitution strategy pave a new pathway for addressing the critical challenges of Ni-rich cathode at high temperatures,which will significantly advance the high-energy-density and high-safety cathodes to the subsequent commercialization.

关键词： Ni-rich cathode Single crystalline dual-substitution strategy High-temperature cathode li-ion batteries

Nano-frictional mechano-reinforcing porous nanowires scaffolds

在线全文

学校读者我要写书评

暂无评论

Friction 2024年第5期12卷 968-980页

作者： licheng HUA Conghu HU Jingkang ZHANG Jin li Chenjie GU Bin HUANG Guangyong li Jianke du Wanlin GUO Smart Materials and Advanced Structures Laboratory School of Mechanical Engineering&MechanicsNingbo UniversityNingbo315211China Key Laboratory for Intelligent Nano Materials and Devices of Ministry of Education Nanjing University of Aeronautics and AstronauticsNanjing210016China Key Laboratory of Impact and Safety Engineering Ministry of EducationSchool of mechanical Engineering and MechanicsNingbo UniversityNingbo315211China Department of Microelectronics and Engineering Ningbo UniversityNingbo315211China

Artificial biomaterials with dynamic mechano-responsive behaviors similar to those of biological tissues have been drawing great *** this study,we report a TiO_(2)-based nanowire(TiO_(2)NWs)scaffolds,which exhibit dynamic mechano-responsive behaviors varying with the number and amplitude of nano-deformation *** is found that the elastic and adhesive forces in the TiO_(2)NWs scaffolds can increase significantly after multiple cycles of *** nanofriction experiments show the triboelectric effect of increasing elastic and adhesive forces during the nano-deformation cycles of TiO_(2)NWs *** properties allow the TiO_(2)NW scaffolds to be designed and applied as intelligent artificial biomaterials to simulate biological tissues in the future.

关键词： TiO_(2)NWs scaffolds nano-deformation mechano-reinforcing triboelectric effect intelligent artificial biomaterials

Robust chemo-mechanical stability of additives-free SiO_(2)anode realized by honeycomb nanolattice for high performance li-ion batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第10期35卷 442-446页

作者： Xinpin Pan Yongjian Cui Zhe Wang Bowen li Hailong Wang Jian Hao Feng li Jing li Advanced Energy Storage Materials Lab School of Materials and New EnergyNingxia UniversityYinchuan 750021China School of Chemistry and Chemical Engineering Ningxia UniversityYinchuan 750021China

Silicon-based anodes including Si,Si Oxand SiO_(2)could deliver ultra-large capacities,but degrade fast owing to huge volume change and low ***,large amounts of elastic binder and conductive additives were composited with nanosized silicon-based materials to yield reasonable cycling stability,which nevertheless not only decrease specific capacity but also induce inhomogeneous lithiation/delithiation as well as uneven stress *** nanolattice has exhibited superior mechanical properties which could be ideal structure for silicon-based anodes,but yet faces challenges in integration of chemical reactivity,conductivity and mechanical ***,we fabricate artificial SiO_(2)honeycomb nanolattice consisting of numerous nanoscale SiO_(2)cells interconnected by through-holes,and conformal coating of highly graphitic carbon on the nanolattice is achieved through in situ catalytic ***,the nanolattice is firmly bonded on Cu substrate through atomic interdiffusion irrespective of surface *** unique structure allows fast charge transportation and homogeneous lithiation/delithiation throughout the micron-meter nanolattice,which results in excellent stability and large reversible capacity over 500 cycles at 1 A/*** results highlight design and constructing artificial nanolattice can be an effective way to prevent chemo-mechanical degradation of silicon-based anode materials.

关键词： li-ion batteries Nanolattice SiO_(2)anode Stability Conductivity