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各向异性黏弹性介质伪谱法波场模拟

物理学报 2004年第12期53卷 4428-4434页

作者：杜启振石油大学(华东)地球资源与信息学院

当地震信号通过复杂地球介质时 ,地层除了表现为各向异性 ,还表现为内在的黏弹性特征因此 ,为准确描述地震波在地球介质中的传播特征 ,理想的地球介质模型应该能够模拟岩石的各向异性特征和衰减特征 .给出了各向异性黏弹性介质模型的... 详细信息

当地震信号通过复杂地球介质时 ,地层除了表现为各向异性 ,还表现为内在的黏弹性特征因此 ,为准确描述地震波在地球介质中的传播特征 ,理想的地球介质模型应该能够模拟岩石的各向异性特征和衰减特征 .给出了各向异性黏弹性介质模型的波动方程 ,推导了伪谱法波场正演模拟的递推公式 ,并利用伪谱法实现了地震波波场数值模拟 .

关键词：伪谱法黏弹性介质介质模型地球波场模拟地震波场各向异性递推公式推导波动方程

Immunofluorescent Labeling of Human HepG2 Cells with CdTe Quantum Dot Probe Conjugated with Anti-pan CK MAb

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Chemical Research in Chinese Universities 2011年第2期27卷 277-281页

作者： SUI Yu-jie ZHANG Gui-zhen WANG Qian WANG Ya-li WU Mei du zhen-wu ZHANG Jie JIANG Ri-hua Central Laboratory China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China Central Laboratory the Second Hospital of Jilin University Changchun 130041 P. R. China Department of Urinary Surgery China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China College of Chemistry Jilin University Changchun 130012 P. R. China

A relatively sensitive, specific, and photostable method for the detection of cytokeratin of cancer cells via conjugation with cadmium telluride quantum dots（CdTe QDs） was described. Water soluble CdTe QDs were conjugated to anti-pan-cytokeratin（CK） monoclonal antibody（MAb） through coupling reagent [1-ethyl-3-（3-dimethyla- mino propyl）carbodiimide, EDC] and the conjugates were purified by dialysis. The expression of pan CK protein in HepG2 cells was observed by immunocytochemistry and direct immunofluorescence via QDs-Ab conjugates respectively. Fluorescence intensity and photostability of QDs were compared with those of FITC（fluorescein isothiocyanate）. The results show that the QDs-Ab conjugates recognized specifically pan CK protein in HepG2 cells. Compared with FITC, CdTe QDs had higher brightness and photostability without obvious photobleaching under continuous exciting light illumination for 30 min and after the placement at room temperature for 3 d. The results indicate that conjugates of CdTe quantum dot with anti-pan CK MAb can be used for labeling cancer cells derived from epithelial tissues, which provides the basis for the detection of circulating tumor cells（CTCs）.

关键词： Quantum dot Pan-cytokeratin Immunofluorescence labeling Fluorescence photostability

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基于卷积神经网络的HTTP隧道检测

信息技术 2024年第7期48卷 160-166页

作者：宋松山黄文培李颖颖杜圳西南交通大学信息科学与技术学院成都611756 西南交通大学计算机与人工智能学院成都611756

超文本传输协议(Hyper Text Transfer Protocol,HTTP)隧道具有穿越防火墙和规避入侵检测系统识别的能力,给信息安全带来严重威胁。然而现阶段的HTTP隧道检测方法识别能力不足、难以应对特征复杂的HTTP隧道。文中分析了HTTP隧道数据包与... 详细信息

超文本传输协议(Hyper Text Transfer Protocol,HTTP)隧道具有穿越防火墙和规避入侵检测系统识别的能力,给信息安全带来严重威胁。然而现阶段的HTTP隧道检测方法识别能力不足、难以应对特征复杂的HTTP隧道。文中分析了HTTP隧道数据包与正常HTTP数据包之间的差别,针对目前HTTP隧道检测方法存在的不足,提出了一种仅需提取小部分流量数据的基于卷积神经网络的HTTP隧道检测方法。实验结果表明,基于卷积神经网络的HTTP隧道检测方法能有效识别网络中的HTTP隧道流量,检测精确率、召回率、F1分数均达到99%以上,且不需要人工选择大量的专家特征,对网络流量监管有重要意义。

关键词：超文本传输协议网络隧道入侵检测信息安全卷积神经网络

Fluorosis Caused Cellular Apoptosis and Oxidative Stress of Rat Kidneys

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Chemical Research in Chinese Universities 2013年第2期29卷 263-269页

作者： SONG Yang WANG Jin-cheng XU Hui du zhen-wu ZHANG Gui-zhen SELIM Hamid Abdu LI Guang-sheng WANG Qing GAO Zhong-li Department of Orthopedics P. R. China Central Laboratory China-Japan Union Hospital of Jilin UniversityChangchun 130033 P. R. China Pathological Laboratory Institute of Endemic Disease of Jilin University Changchun 130021 P. R. China

As the strongest electronegative element, fluorine can stimulate the production of superoxide radicals in cells. In view of the important roles of kidneys in bone metabolism, the authors analyzed the quantitative pathomor- phological characteristics of renal damage and the potential cellular apoptosis and oxidative stress mechanisms in rats treated with excessive fluoride. Wistar rats were exposed to 50 mg F （110.5 mg NaF）/L, 100 mg F-（221.0 mg NaF）/L and 150 mg F-（331.5 mg NaF）/L in drinking water for 70 and 140 d, respectively. Microscope with image analysis was used to quantitate pathomorphological changes in renal tissues of the rats. Reactive oxygen species（ROS）, the cell cycle and apoptosis of renal cells were measured by flow cytometry and TUNEL technique（terminal deoxynuc- leotidyl transferase duTP nick end labeling）, respectively. The ion concentrations in serum and renal functional pa- rameters were detected by automatic biochemical analyzer. Quantitative analysis results demonstrate the expanded Bowman＇s space of glomerulus and obvious dilatation of renal tubule. TUNEL technique revealed that NBT/BCIP （nitro blue tetrazolium/5-bromo-4-chloro-3＇-indolylphosphate, p-toluidine salt）-staining positive apoptotic cells se- lectively located in medullocortical junction areas. The data suggest that renal damage in chronic fluorostic rats is associated with the cellular apoptosis and oxidative stress.

关键词： Fluorosis Apoptosis Oxidative stress

电针联合等速训练对THA术后髋关节生物力学的影响

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中华老年骨科与康复电子杂志 2024年第2期10卷 103-110页

作者：孙阳杨帅贾晋瑄杜震周凌峰魏贤振上海中医药大学附属市中医医院康复医学科 200071 上海交通大学医学院附属瑞金医院康复医学科 200025 上海中医药大学附属市中医医院医务处 200071 上海中医药大学附属市中医医院骨伤科 200071

目的探究电针联合等速肌力训练对全髋关节置换术后患者髋关节生物力学的影响。方法选取全髋关节置换术后患者72例,采用完全随机化方法分为观察组和对照组各36例(观察组脱失1例)。观察组采用电针联合等速肌力训练疗法,对照组采用传统康... 详细信息

目的探究电针联合等速肌力训练对全髋关节置换术后患者髋关节生物力学的影响。方法选取全髋关节置换术后患者72例,采用完全随机化方法分为观察组和对照组各36例(观察组脱失1例)。观察组采用电针联合等速肌力训练疗法,对照组采用传统康复训练疗法。于术后3个月时对所有患者进行等速肌力测定并记录各项数据。结果共72例患者获得随访,随访时间3个月。其中男性22例,女性49例,平均年龄(75.17±10.49)岁。术后3个月时,观察组患者健侧伸肌群PT[(79.11±7.11)N·m,t=2.589,P=0.012],患侧屈肌群PT[(73.23±9.40)N·m,t=2.158,P=0.034]、PTA[(73.00±18.58)°,t=3.020,P=0.004]、TW[(113.80±39.75)J,t=2.395,P=0.019],伸肌群PT[(67.09±8.69)N·m,t=3.035,P=0.003]、PT/BW[(108.27±23.50)%N·m/Kg,t=2.252,P=0.028],健侧内收肌群AP[(14.09±3.72)W,t=0.239,P=0.020]、患侧外展肌群PT[(61.43±15.02)N·m,t=4.158,P=0.000]、PT/BW[(98.25±26.25)%N·m/Kg,t=3.721,P=0.000]、PTA[(29.11±11.06)°,t=2.011,P=0.048]、TW[(121.29±33.21)J,t=6.204,P=0.000],内收肌群PT[(48.54±15.92)N·m,2.768,P=0.007]、PT/BW[(78.70±28.93)%N·m/Kg,t=2.531,P=0.014]、TW[(50.43±15.16)J,t=7.186,P=0.000],以上指标均具有统计学差异(P<0.05);患侧与健侧指标比较,除观察组屈肌群TW[(113.80±39.75)J,t=5.258,P=0.264]、外展肌群TW[(121.29±33.21)J,t=2.467,P=1.552]、内收肌群TW[(50.43±15.16)J,t=7.176,P=0.089],对照组伸肌群PTA[(85.42±16.00)°,t=3.099,P=5.599]、伸肌群TW[(122.33±35.41)J,t=2.965,P=0.157]外,其他指标差异均有统计学意义(P<0.05)。结论通过分析髋关节生物力学特征证实,电针联合IST有助于改善THA术后患者髋周各肌群肌力,增强髋关节稳定性,值得临床广泛推广使用。

关键词：电针等速肌力训练全髋关节置换术生物力学

MicroRNA-mediated Silencing of RhoC Inhibits Tumor Invasion and Increases Chemosensitivity to Paclitaxel in SKOV3 Cells in vitro

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Chemical Research in Chinese Universities 2011年第1期27卷 70-74页

作者： PAN Ying du zhen-wu LENG Wei-chun ZHOU Jia-wen WANG Ying-jian SHENG Min-jia WANG Jun-rong ZHANG Gui-zhen China-Japan Union Hospital of Jilin University Changchun 130031 P. R. China

RhoC is a member of the Ras-homologous family of genes which are implicated in tumorigenesis and tumor progression. Up-regulation of RhoC is associated with tumor progression in ovarian carcinoma and RhoC is significantly correlated with the invasive capability of ovarian cancer cell lines in vitro, We developed a system that blocks RhoC in the human ovarian cancer SKOV3 cells using specific MicroRNA（miRNA） interference. By transfecting SKOV3 cells with the plasmid vector to express specific MiRNA that targets human RhoC, we were able to establish a stable clone in which RhoC expression was significantly downregulated. This resulted in the decreased invasive potential of SKOV3 cells as well as increased chemosensitivity to paclitaxel. RhoC involves in invasion and chemosensitivity of SKOV3, indicating that RhoC may be a promising therapeutic target for ovarian cancer.

关键词： RhoC gene miRNA SKOV3 cell Cell invasion Paclitaxel

人体成分分析在住院精神疾病患者营养诊断中的应用研究

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河北医科大学学报 2024年第8期45卷 968-973页

作者：宋世明胡环宇杜红珍李增宁河北医科大学第一医院临床营养科河北省营养与健康重点实验室河北石家庄050031

目的探讨人体成分分析在住院精神疾病患者营养诊断中的作用。方法纳入住院精神疾病患者100例为病例组,同时招募与病例组年龄、性别等相匹配的健康者100例为对照组。利用生物电阻抗技术进行人体成分检测,并对比分析各组的检测结果。结果... 详细信息

目的探讨人体成分分析在住院精神疾病患者营养诊断中的作用。方法纳入住院精神疾病患者100例为病例组,同时招募与病例组年龄、性别等相匹配的健康者100例为对照组。利用生物电阻抗技术进行人体成分检测,并对比分析各组的检测结果。结果病例组男性与女性的身体质量指数(21.05 vs.24.30;18.40 vs.23.30)、蛋白质(9.40 vs.10.70;6.85 vs.7.95)、无机盐(3.02 vs.3.74;2.48 vs.2.94)、骨矿物质含量(2.46 vs.3.10;2.05 vs.2.47)、上臂围度(28.85 vs.31.10;25.05 vs.28.60)、相位角(4.95 vs.6.00;4.70 vs.5.19)、身体细胞量(24.65 vs.35.45;24.50 vs.26.45)、细胞内水分比率(0.61 vs.0.62;0.61 vs.0.62)、去脂体重(38.25 vs.53.95;38.05 vs.40.75)、骨骼肌质量(20.45 vs.30.30;20.30 vs.22.10)、无脂肪质量指数(14.39 vs.18.80;14.57 vs.15.70)、骨骼肌质量指数(7.58 vs.10.50;7.78 vs.8.55)及身体脂肪量(11.45 vs.15.60;12.60 vs.17.95)分别低于对照组(P<0.05),而细胞外水分比率(0.39 vs.0.38;0.39 vs.0.38)高于对照组(P<0.05);病例组与对照组中,男性与女性的相位角与细胞内水分、细胞内外水分比率、蛋白质、骨骼肌质量及身体细胞量均呈正相关(P<0.05)。结论病例组男性与女性的人体成分分析结果较对照组差,因此可用于住院精神疾病患者的营养诊断。

关键词：精神病性障碍人体成分分析相位角

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Effect of Regulation of HSV-tk Gene Expression and Tumor Killed Activity with a Single Tetracycline-regulatable Plasmid Vector on HeLa Cells

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Chemical Research in Chinese Universities 2009年第5期25卷 677-680页

作者： WANG Qian du zhen-wu MA Qing-shan ZHANG Yu-cheng WU Xiao-dong YANG Shao-juan WANG Ya-li ZHANG Gui-zhen Central Laboratory China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Department of Urinary Surgery China-Japan Union Hospital Jilin University Changchun 130033 P. R. China School of Pharmaceutical Sciences The First Hospital Jilin University Changehun 130021 P. R. China Department of Pediatrics The First Hospital Jilin University Changehun 130021 P. R. China

To construct a single tetracycline-regulatable plasmid vector based on the double tetracycline-regulatable plasmid vector system for regulating HSV-tk gene expression so as to effectively kill HeLa cells. Two tetracycline operator（TetO2） was cloned into pcDNA3.1 and a cassette was made for a cytomegalovirus-type 2 tetracycline operator（CMV-TetO2） promoter, and the obtained vector was named pcDNA3.1-CMV-TetO2. Herpes simplex virus thymidine kinase（HSV-tk） gene and tetracycline repressor（TR） gene were cloned into pcDNA3.1-CMV-TetO2 and the two genes were linked with internal ribosome entry site（IRES） to gain a vector named pcDNA3.1-CMV-TetO2- HSV-tk-IRES-TR. The HeLa cells were stablly transfected with pcDNA3.1-CMV-TetO2-HSV-tk-IRES-TR plasmid. The expression of HSV-tk and TR were detected by RToPCR, the tumorcidal activity of HSV-tk/GCV was determined by MTT assay. In Hela cells transfected with the above plasmid vector, HSV-tk gene and TR gene can be expressed lowly and the concentration of GCV producing a 50% decrease in cell viability was about 50 lag/mL without adding deoxycycline; in contrast, the expessions of HSV-tk gene and TR gene increased significantly and the concentration of GCV producing a 50% decrease in cell viability was about 5 μg/mL with adding deoxycycline. Therefore tetracycline can regulate the expression and tumorcidal activity of HSV-tk gene in HeLa cells with this single plasmid vector.

关键词： HSV-tk gene Tetracycline HeLa cells Gene expression

Involvement of Plasma Membrane Ca^(2+)/H^+ Antiporter in Cd^(2+) Tolerance

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Rice science 2012年第2期19卷 161-165页

作者： SHEN Guo-ming du Qi-zhen WANG Jiang-xin School of Food Science and Biotechnology Zhejiang Gongshang University 149 Jiaogong Road Hangzhou 310012 China Center for Biosignature Discovery Automation Arizona State University Tempe AZ 85287 USA

Cation exchangers （CAXs） belong to the cation/Ca2＋exchanger superfamily which have been extensively investigated in plant tonoplasts over the last decade. Recently, the roles of CAXs involved in heavy metal accumulation and tolerance in plants have been studied for phytoremediation and food security. In this mini review, we summarize the roles of the Ca2＋/H＋ antiporter in Ca2＋ signal transduction, maintaining ion homeostasis and sequestering heavy metals into the vacuole. Moreover, we present a possible role of the plasma membrane Ca2＋/H＋ antiporter in heavy metal detoxification.

关键词： Ca2+/H+ antiporter Cd2+ detoxification heavy metal plasma membrane rice