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Assignment of unanchored scaffolds in genome of Brassica napus by RNA-seq analysis in a complete set of Brassica rapa-Brassica oleracea monosomic addition lines

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Journal of Integrative Agriculture 2019年第7期18卷 1541-1546页

作者： HUO Dong-ao ZHU Bin TIAN Gui-fu du xu-ye GUO Juan CAI Meng-xian School of Life Sciences Buckwheat Research CenterGuizhou Normal University

The economically valuable oil crop Brassica napus(AACC,2 n=38),which arose from interspecific hybridization between the diploid ancestors Brassica rapa(AA,2 n=20) and Brassica oleracea(CC,2 n=18),has a complex *** than 10% of the assembled sequences,most of which belong to the C subgenome,have not been anchored to the corresponding ***,a complete set of monosomic alien addition lines(MAALs,C1–C9) with each of the nine C-subgenome chromosomes added to the extracted A subgenome was obtained from the allotetraploid *** donor Oro,after the ancestral ***(RBR Oro) genome was *** MAALs effectively reduced the complexity of the *** ***,we determined the expression values of genes on unanchored scaffolds in the MAALs and RBR ***,multiple comparisons of these gene expression values were used to determine the affiliations of the nonanchored scaffolds on which the genes were *** total,54.68%(44.11 Mb) of the 80.67 Mb of non-anchored scaffolds belonging to the C subgenome were assigned to corresponding C *** work highlights the potential value of these MAALs in improving the genome quality of ***.

关键词： Brassica napus genome sequence RNA-seq monosomic alien addition lines scaffolds

Association analysis of grain traits with SSR markers between Aegilops tauschii and hexaploid wheat(Triticum aestivum L.)

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Journal of Integrative Agriculture 2015年第10期14卷 1936-1948页

作者： ZHAO Jing-lan WANG Hong-wei ZHANG Xiao-cun du xu-ye LI An-fei KONG Ling-rang State Key Laboratory of Crop Biology College of Agronomy Shandong Agricultural University Taishan Polytechnic

Seven important grain traits, including grain length（GL）, grain width（GW）, grain perimeter（GP）, grain area（GA）, grain length/width ratio（GLW）, roundness（GR）, and thousand-grain weight（TGW）, were analyzed using a set of 139 simple sequence repeat（SSR） markers in 130 hexaploid wheat varieties and 193 Aegilops tauschii accessions worldwide. In total, 1 612 alleles in Ae. tauschii and 1 360 alleles in hexaploid wheat（Triticum aestivum L.） were detected throughout the D genome. 197 marker-trait associations in Ae. tauschii were identified with 58 different SSR loci in 3 environments, and the average phenotypic variation value（R2） ranged from 0.68 to 15.12%. In contrast, 208 marker-trait associations were identified in wheat with 66 different SSR markers in 4 environments and the average phenotypic R2 ranged from 0.90 to 19.92%. Further analysis indicated that there are 6 common SSR loci present in both Ae. tauschii and hexaploid wheat, which are significantly associated with the 5 investigated grain traits（i.e., GA, GP, GR, GL, and TGW） and in total, 16 alleles derived from the 6 aforementioned SSR loci were shared by Ae. tauschii and hexaploid wheat. These preliminary data suggest the existence of common alleles may explain the evolutionary process and the selection between Ae. tauschii and hexaploid wheat. Furthermore, the genetic differentiation of grain shape and thousand-grain weight were observed in the evolutionary developmental process from Ae. tauschii to hexaploid wheat.

关键词： association analysis grain traits Aegilops tauschii Triticum aestivum SSR markers

Enantio-divergent C–H borylation with two different ligands from a single chiral source

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Science China Chemistry 2025年第1期68卷 226-232页

作者： Rongrong du Senmiao xu State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China College of Material Chemistry and Chemical Engineering Key Laboratory of Organosilicon Chemistry and Material TechnologyMinistry of EducationHangzhou Normal UniversityHangzhou 311121China University of Chinese Academy of Sciences Beijing 100049China

Reported here is an iridium-catalyzed enantio-divergent C(sp^(3))–H borylation ofα-gem-dimethyl groups of trifluoromethyl ketone-derived hydrazones to construct a series of acyclic all-carbon quaternary stereocenters with a high level of chiral *** different chiral bidentate boryl ligands from a common chiral source not only can switch the enantioselectivity but also are complementary in terms of the substrate scope.

关键词： asymmetric catalysis C–H borylation all-carbon quaternary stereocenters synthetic methods

Cloning and characterization of a novel UDP-glycosyltransferase gene induced by DON from wheat

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Journal of Integrative Agriculture 2015年第5期14卷 830-838页

作者： MA Xin du xu-ye LIU Guo-juan YANG Zai-dong HOU Wen-qian WANG Hong-wei FENG De-shun LI An-fei KONG Ling-rang State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology College of AgronomyShandong Agricultural University

Fusarium head blight（FHB）,caused primarily by Fusarium graminearum,is a destructive disease of wheat throughout the ***,the mechanisms of host resistance to FHB are still largely ***（DON）produced by F graminearum which enhances the pathogen to spread could be converted into inactive form D3 G by UDP-glycosyltransferases（UGTs）.A DON responsive UGT gene,designated as TaUGT4,was first cloned from wheat in this *** putative open reading frame（ORF） of TaUGT4 was 1386 bp,encoding 461 amino acids ***4 was placed on chromosome 2D using a set of nulli-tetrasomic lines of wheat cultivar Chinese Spring（CS）.When fused with eGFP at C terminal,TaUGT4 was shown to localize in cytoplasm of the transformed tobacco *** transcriptional analysis revealed that TaUGT4 was strongly induced by F graminearum or DON in both of FHB-resistant cultivar Sumai 3 and susceptible cultivar Kenong 199,especially in Sumai 3 under DON *** increase of TaUGT4 expression was observed in Sumai 3 and Kenong 199 in response to salicylic acid（SA） *** interestingly,the transcripts level of TaUGT4 in Sumai 3 showed significantly higher than that in Kenong 199 after treated with methyl jasmonate（MeJA）.According to the expression patterns,TaUGT4 might lead to different effects between FHB-resistant genotype and susceptible genotype in the process against F graminearum *** had also been discussed in this paper that JA signaling pathway might play a significant role in the resistance against *** compared to SA signaling pathway.

关键词： wheat cultivar chromosome designated genotype blight tobacco responsive susceptible pathogen

Functional analysis of a wheat pleiotropic drug resistance gene involved in Fusarium head blight resistance

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Journal of Integrative Agriculture 2016年第10期15卷 2215-2227页

作者： WANG Gui-ping HOU Wen-qian ZHANG Lei WU Hong-yan ZHAO Lan-fei du xu-ye MA Xin LI An-fei WANG Hong-wei KONG Ling-rang State Key Laboratory of Crop Biology/College of Agronomy Shandong Agricultural University College of Life Science Guizhou Normal University College of Horticulture and Forestry Sciences Huazhong Agricultural University

The pleiotropic drug resistance（PDR） sub-family of adenosine triphosphate（ATP）-binding cassette（ABC） transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight（FHB） resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with Ae PDR7 and Bd PDR7. Subcellular localization analysis showed that Ta PDR7 was expressed on the plasmalemma. The quantitative real time PCR（RT-PCR） analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol（DON） in spike. When knocking down the transcripts of all Ta PDR7 members by barely stripe mosaic virus-induced gene silencing（BSMV-VIGS） system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that Ta PDR7 might play an important role in response to F. graminearum. Although salicylic acid（SA）, methyl jasmonate（Me JA） and abscisic acid（ABA） had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of Ta PDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid（IAA）.

关键词： PDR FHB VIGS

Pyroptosis,ferroptosis,and autophagy in spinal cord injury:regulatory mechanisms and therapeutic targets

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Neural Regeneration Research 2025年第10期20卷 2787-2806页

作者： Qingcong Zheng du Wang Rongjie Lin Weihong xu Department of Spinal Surgery the First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Arthritis Clinical and Research Center Peking University People’s HospitalBeijingChina Department of Orthopedic Surgery Fujian Medical University Union HospitalFuzhouFujian ProvinceChina

Regulated cell death is a form of cell death that is actively controlled by *** studies have shown that regulated cell death plays a key role after spinal cord *** and ferroptosis are newly discovered types of regulated cell deaths that have been shown to exacerbate inflammation and lead to cell death in damaged spinal ***,a complex form of cell death that is interconnected with various regulated cell death mechanisms,has garnered significant attention in the study of spinal cord *** injury triggers not only cell death but also cellular survival *** signaling pathways play pivotal roles in influencing the processes of both deterioration and repair in spinal cord injury by regulating pyroptosis,ferroptosis,and ***,this review aims to comprehensively examine the mechanisms underlying regulated cell deaths,the signaling pathways that modulate these mechanisms,and the potential therapeutic targets for spinal cord *** analysis suggests that targeting the common regulatory signaling pathways of different regulated cell deaths could be a promising strategy to promote cell survival and enhance the repair of spinal cord ***,a holistic approach that incorporates multiple regulated cell deaths and their regulatory pathways presents a promising multi-target therapeutic strategy for the management of spinal cord injury.

关键词： autophagy cell death ferroptosis inflammation pathological mechanisms pyroptosis regulated cell death regulatory pathways spinal cord injury therapeutic targets

Let-7 microRNA targets BmCentrin to modulate the development and functionality of the middle silk gland in the silkworm,Bombyx mori

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Insect Science 2025年第1期32卷 95-114页

作者： Yankun xu Na du Lili xu Lu Zhao Ting Fan Tianqi Wei Qian Pu Shiping Liui State Key Laboratory of Resource Insects Southwest UniversityChongqingChina

The silk gland of the silkworm Bombyx mori serves as a valuable model for investigating the morphological structure and physiological functions of *** studies have demonstrated the notable regulatory role of let-7 microRNA in the silk gland,but its specific molecular mechanism remains to be elucidated across different segments of this *** this study,we further investigated the functional mechanism of let-7 in the middle silk gland(MSG).The MSG of a let-7 knockout strain was analyzed using a combined proteomic and metabolomic technique,revealing the enrichment of differential proteins and metabolites in the DNA synthesis and energy metabolism *** was identified as a novel target gene of let-7 in the MSG,and its downregulation inhibited the proliferation of BmN4-SID1 cells,which is exactly opposite to the role of let-7 in these ***/Cas9 genome editing and transgenic technologies were employed to manipulate BmCentrin in the *** of BmCentrin led to severe MSG atrophy,whereas the overexpression of BmCentrin resulted in beaded *** measurements of these knockout or overexpression strains revealed significant changes in the expression levels of sericin protein genes,the weight of the cocoon and the mechanical properties of the *** the biological role of BmCentrin in the silk gland offers valuable insights for elucidating the molecular mechanisms by which let-7 controls silk gland development and silk protein synthesis in the silkworm.

关键词： BmCentrin Bombyx mori CRISPR/Cas9 genome editing let-7 microRNA middle silk gland transgenic overexpression

The burden of upper motor neuron involvement is correlated with the bilateral limb involvement interval in patients with amyotrophic lateral sclerosis:a retrospective observational study

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Neural Regeneration Research 2025年第5期20卷 1505-1512页

作者： Jieying Wu Shan ye Xiangyi Liu Yingsheng xu Dongsheng Fan Department of Neurology Peking University Third HospitalBeijingChina Beijing Municipal Key Laboratory of Biomarker and Translational Research in Neurodegenerative Diseases BeijingChina Key Laboratory for Neuroscience National Health Commission/Ministry of EducationPeking UniversityBeijingChina

Amyotrophic lateral sclerosis is a rare neurodegenerative disease characterized by the involvement of both upper and lower motor *** bilateral limb involvement significantly affects patients'daily lives and may lead them to be confined to ***,the effect of upper and lower motor neuron impairment and other risk factors on bilateral limb involvement is *** address this issue,we retrospectively collected data from 586 amyotrophic lateral sclerosis patients with limb onset diagnosed at Peking University Third Hospital between January 2020 and May 2022.A univariate analysis revealed no significant differences in the time intervals of spread in different directions between individuals with upper motor neuron-dominant amyotrophic lateral sclerosis and those with classic amyotrophic lateral *** used causal directed acyclic graphs for risk factor determination and Cox proportional hazards models to investigate the association between the duration of bilateral limb involvement and clinical baseline characteristics in amyotrophic lateral sclerosis *** factor analyses revealed that higher upper motor neuron scores(hazard ratio[HR]=1.05,95%confidence interval[CI]=1.01–1.09,P=0.018),onset in the left limb(HR=0.72,95%CI=0.58–0.89,P=0.002),and a horizontal pattern of progression(HR=0.46,95%CI=0.37–0.58,P<0.001)were risk factors for a shorter interval until bilateral limb *** results demonstrated that a greater degree of upper motor neuron involvement might cause contralateral limb involvement to progress more quickly in limb-onset amyotrophic lateral sclerosis *** findings may improve the management of amyotrophic lateral sclerosis patients with limb onset and the prediction of patient prognosis.

关键词： amyotrophic lateral sclerosis bilateral limb involvement Cox proportional hazards regression model horizontal spread restricted cubic spline analysis time interval upper motor neuron vertical spread

Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru ye Liyan Zhong Yashuang xu Junhui Ren Yi Liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission