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Effect of moxibustion on expression profile of miRNAs in Tripterygium glycoside-induced decreased ovarian reserve

Journal of Traditional Chinese Medicine 2024年第4期44卷 745-752页

作者： SHEN Jie YIN Yaoli LI Hongxiao LU Ge ZHU Yaoyao QIN Yantong JIN Xun CHENG Jie SHEN meihong College of Acupuncture and Tuina Nanjing University of Chinese MedicineNanjing 210023China

OBJECTIVE:To explore the possible regulatory mechanism of microRNA(miRNA)in moxibustion treatment for decreased ovarian reserve(DOR).METHODS:The DOR model was constructed by intragastrical Tripterygium glycoside suspension administration,and moxibustion therapy was simultaneously *** morphological ovarian changes were observed by hematoxylin and eosin *** miRNA expression profile was detected by RNA sequencing,and bioinformatics analysis was *** software 3.6.1 was used to establish a regulatory network and differentially expressed miRNAs were verified by reverse transcription quantitative polymerase chain reaction(RT-qPCR).RESULTS:Decreased number of mature follicles,increased atresia follicles,and abnormal granulosa cell morphology were observed in the model group compared with the control *** moxibustion group demonstrated increased mature follicles,decreased atretic follicles,and significantly decreased abnormal morphology of granulosa cells compared with the model ***,RNA sequencing results manifested significantly up-regulated miRNA expressions(miR-92b-3p,miR-26-5p_R+1_1ss10TC,miR-206-3p,miR-9993b-3p_1ss6GA,miR-7857-3p_R-1,miR-219a-2-3p_1ss10GC,miR-3968-p5_1ss10AT,and PC-5p-6478_1795)and down-regulated miR-664-2-5p_R+1 in the model group,compared with the control group,and the moxibustion group reversed abnormal disorder levels of these ***,these differentially expressed miRNAs were mainly involved in the phosphatidylinositol-3-kinase/protein kinase B signaling pathway and nuclear factor erythropoietin-2-related factor 2/heme oxygenase 1 signaling ***,network and RT-qPCR verification revealed miR-9993b-3p_1ss6GA as the most critical ***:This experiment proved the effectiveness of moxibustion in improving the ovarian reserve of rats by regulating miRNA expression,especially miR-9993b-3p_1ss6GA.

关键词： ovarian reserve moxibustion microRNAs mechanics

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禽类卵母细胞发育观察方法的改进

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中国家禽 2009年第2期31卷 51-52页

作者：杜美红蒋斌李赞东北京市理化分析测试中心北京100089 中国农业大学生物学院北京100094

以往关于卵母细胞的发育研究通常采用局部卵巢组织切片来研究卵母细胞的生长发育情况，随着细胞生物学的发展和体外培养技术的进步，通过体外细胞培养对卵母细胞生长发育研究的进一步深入，对于卵母细胞结构尤其是其生长发育中一些基因... 详细信息

以往关于卵母细胞的发育研究通常采用局部卵巢组织切片来研究卵母细胞的生长发育情况，随着细胞生物学的发展和体外培养技术的进步，通过体外细胞培养对卵母细胞生长发育研究的进一步深入，对于卵母细胞结构尤其是其生长发育中一些基因表达的产物和调控激素类物质的作用成为人们研究的热点之一。本研究提供了一种观察体外培养卵母细胞发育进程的新方法。

关键词：卵母细胞发育观察方法体外细胞培养禽类生长发育细胞生物学组织切片培养技术

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Inhalable iron redox cycling powered nanoreactor for amplified ferroptosis-apoptosis synergetic therapy of lung cancer

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Nano Research 2024年第6期17卷 5435-5451页

作者： Linjing Wu Wenhao Wang Mengqin Guo Fangqin Fu Wenhua Wang Tszching Sung meihong Zhang Ziqiao Zhong Chuanbin Wu Xin Pan Zhengwei Huang College of Pharmacy Jinan UniversityGuangzhou 511436China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China

Fenton reaction centered ferroptosis-apoptosis synergetic therapy has emerged as a promising tumor elimination ***,the low intracellular Fenton level and accumulation of therapeutics at the lesion site greatly limit the efficacy of ferroptosis *** overcome these two bottlenecks,an inhalable metal polyphenol network(MPN)-hybrid liposome,encoded as LDG,was proposed for enhancing the intracellular Fenton reaction level by co-delivering the ferroptosis inducer dihydroartemisinin(DHA)and the ferrous ion(Fe2+)donor *** synthesized LDG had excellent nebulization performance which significantly improved the accumulation in the lungs,about 8.2 times of intravenous *** terms of anticancer mechanisms,MPN raised the intracellular level of Fe2+by constructing iron cycling in the weakly acidic environment of *** by Fe2+,DHA with peroxide-bridge structure underwent a high level of Fenton-like reaction,promoted the production of intracellular reactive oxygen species(ROS)and induced strong ferroptosis while cooperating with *** exhibited extraordinary antitumor ability in an orthotopic lung tumor model,whose tumor inhibition efficiency was 1.53(P=0.0014)and 1.32(P=0.0183)times of the LG group(liposomes coated with gallic acid(GA)-Fe MPN)and LD group(liposomes loaded with DHA),respectively,showing the strongest anticancer *** conclusion,the constructed MPN-hybrid liposomes could be a potent custom nanoplatform for pulmonary delivery and underscored the great potential of ferroptosis-apoptosis synergetic therapy.

关键词： lung cancer pulmonary delivery ferroptosis metallic polyphenol network

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Protective eff ect and mechanism of nanoantimicrobial peptide ND-C14 against Streptococcus pneumoniae infection

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World Journal of Emergency Medicine 2024年第1期15卷 28-34页

作者： Yangyang Guo Yaqi Sun Hongxia Wu Jian Lu Yuan Lin Jiaqing Zhu meihong Lai Meiqi Zhang Jun Wang Jungang Zheng Department of Intensive Care Medicine Hangzhou Traditional Chinese Medicine Hospital Affi liated to Zhejiang Chinese Medical UniversityHangzhou 310021China National Children’s Regional Medical Center National Clinical Research Center for Child HealthChildren’s HospitalZhejiang University School of MedicineHangzhou 310052China Department of Spleen and Stomach Diseases Hangzhou Traditional Chinese Medicine Hospital Affi liated to Zhejiang Chinese Medical UniversityHangzhou 310021China Department of Ultrasonic Medicine the First Affi liated Hospital of Ningbo UniversityNingbo 315010China Department of Anesthesiology the First Affi liated Hospital of Ningbo UniversityNingbo 315010China Zhejiang University School of Medicine Hangzhou 310058China The First People’s Hospital of Yuexi County Yuexi 616650China

BACKGROUND:Streptococcus pneumoniae(***)is a common pathogen that causes bacterial ***,with increasing bacterial resistance,there is an urgent need to develop new drugs to treat *** *** with a 14-carbon saturated fatty acid(ND-C14)is a novel nanoantimicrobial peptide designed by modifying myristic acid at the C-terminus of humanα-defensin 5(HD5)via an amide ***,it is unclear whether ND-C14 is effective against lung infections caused by ***:In vitro,three groups were established,including the control group,and the HD5 and ND-C14 treatment groups.A virtual colony-count assay was used to evaluate the antibacterial activity of HD5 and ND-C14 against *** morphological changes of *** treated with HD5 or ND-C14 were observed by scanning electron *** vivo,mice were divided into sham,vehicle,and ND-C14 treatment *** in the sham group were treated with 25μL of phosphate-buffered saline(PBS).Mice in the vehicle and ND-C14 treatment groups were treated with intratracheal instillation of 25μL of bacterial suspension with 2×108 CFU/mL(total bacterial count:5×10^(6) CFU),and then the mice were given 25μL PBS or intratracheally injected with 25μL of ND-C14(including 20μg or 50μg),*** rates were evaluated in the vehicle and ND-C14 treatment *** burden in the blood and bronchoalveolar lavage fluid were *** lung histology of the mice was assessed.A propidium iodide uptake assay was used to clarify the destructive eff ect of ND-C14 against ***:Compared with HD5,ND-C14 had a better bactericidal eff ect against *** because of its stronger ability to destroy the membrane structure of *** in *** vivo,ND-C14 significantly delayed the death time and improved the survival rate of mice infected with ***-C14 reduced bacterial burden and lung tissue ***,ND-C14 had a membrane per

关键词： Streptococcus pneumoniae Antimicrobial peptides Humanα-defensin 5 Infection

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High-stability double-layer polymer-inorganic composite electrolyte fabricated through ultraviolet curing process for solid-state lithium metal batteries

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Frontiers of Materials Science 2024年第2期18卷 117-128页

作者： Xinghua Liang Pengcheng Shen Lingxiao Lan Yunmei Qin Ge Yan meihong Huang Xuanan Lu Qiankun Hun Yujiang Wang Jixuan Wang Guangxi Key Laboratory of Automobile Components and Vehicle Technology Guangxi University of Science&TechnologyLiuzhou 545006China Guangxi Automobile Group Co. Ltd.Liuzhou 545006China School of Automotive Engineering Guangdong Polytechnic of Industry and CommerceGuangzhou 510510China

Electrolyte interface resistance and low ionic conductivity are essential issues for commercializing solid-state lithium metal batteries(SSLMBs).This work details the fabrication of a double-layer solid composite electrolyte(DLSCE)for *** composite comprises poly(vinylidene fluoride-co-hexafluoropropylene)(PVDF-HFP)and poly(methyl methacrylate)(PMMA)combined with 10 wt.%of Li_(6.4)La_(3)Zr_(1.4)Ta_(0.6)O_(12)(LLZTO),synthesized through an ultraviolet curing *** ionic conductivity of the DLSCE(2.6×10^(-4) S·cm^(-1))at room temperature is the high lithium-ion transference number(0.57),and the tensile strength is 17.8 *** this DLSCE was assembled,the resulted LFP/DLSCE/Li battery exhibited excellent rate performance,with the discharge specific capacities of 162.4,146.9,93.6,and 64.0 mA·h·g^(-1) at 0.1,0.2,0.5,and 1 C,***,the DLScE demonstrates remarkable stability with lithium metal batteries,facilitating the stable operation of a Li/Li symmetric battery for over 200 h at both 0.1 and 0.2 mA-cm^(-2).Notably,the formation of lithium dendrites is also effectively inhibited during *** work provides a novel design strategy and preparation method for solid composite electrolytes.

关键词： electrochemicalreliability lithium metal battery lithium-ion transference number double-layer solid composite electrolyte

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国产分析仪器发展现状与对策分析——以国产离子色谱仪为例

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中国科技资源导刊 2012年第5期44卷 103-106页

作者：高越杜美红北京市科学技术研究院北京100089 北京市理化分析测试中心北京100089

为探讨我国科学仪器现状及未来发展，本文以国产科学仪器离子色谱仪为例，通过对其输液系统、进样系统、抑制系统、检测系统等各组成部分性能进行分析，客观了解并认识国产科学仪器整体发展水平，揭示国产科学仪器目前存在的核心问题，... 详细信息

为探讨我国科学仪器现状及未来发展，本文以国产科学仪器离子色谱仪为例，通过对其输液系统、进样系统、抑制系统、检测系统等各组成部分性能进行分析，客观了解并认识国产科学仪器整体发展水平，揭示国产科学仪器目前存在的核心问题，提出加强产一学一研一用相结合模式，加强科学仪器基础研究，开展国产科学仪器应用示范是国产科学仪器未来发展必然之路。

关键词：国产科学仪器化学分析仪器离子色谱仪科学仪器研发性能应用示范

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国产科学仪器在食品安全检测中的应用现状

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现代科学仪器 2008年第5期25卷 3-6页

作者：杜美红陈舜琮北京市理化分析测试中心北京100089

国产科学仪器发展迅速、应用广泛。本文主要从5个方面综述了国产光谱仪、色谱仪、质谱仪、电化学仪、生命科学仪器等科学仪器在食品安全检测中的应用现状与发展趋势,同时,指出了国产科学仪器在资金投入、技术和质量等方面还存在着一些问... 详细信息

国产科学仪器发展迅速、应用广泛。本文主要从5个方面综述了国产光谱仪、色谱仪、质谱仪、电化学仪、生命科学仪器等科学仪器在食品安全检测中的应用现状与发展趋势,同时,指出了国产科学仪器在资金投入、技术和质量等方面还存在着一些问题,最后对国产科学仪器将来的发展方向提出了展望。

关键词：国产仪器食品安全光谱仪色谱仪电化学仪器转基因仪器

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链路及节点多属性融合的低轨卫星网络路由算法

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计算机工程与应用 2024年第18期60卷 266-274页

作者：孙正阳杜晔李桂领黎妹红北京交通大学智能交通数据安全与隐私保护技术北京市重点实验室北京100044 北京交通大学计算机与信息技术学院北京100044 教育部教育考试院北京100084

针对低轨卫星网络面对破坏性事件系统性能损失多、通信延迟和丢包率高的问题,提出了基于多属性的低轨卫星网络多路径路由算法。在采用虚拟拓扑方案仿真的低轨卫星星座中,计算源节点与目标节点之间的多条可行路径,该算法融合卫星间链路... 详细信息

针对低轨卫星网络面对破坏性事件系统性能损失多、通信延迟和丢包率高的问题,提出了基于多属性的低轨卫星网络多路径路由算法。在采用虚拟拓扑方案仿真的低轨卫星星座中,计算源节点与目标节点之间的多条可行路径,该算法融合卫星间链路属性和相邻卫星节点属性构建路径的传输成本模型,自适应调整两类属性对传输成本的影响权重,每颗卫星将路径传输成本的归一化结果作为通过该路径转发报文的概率,并独立进行分布式的路由选择。仿真结果表明,与经典的低轨卫星网络多路径路由算法相比,无论网络负载较大发生拥塞时,还是卫星遭受网络攻击或出现故障时,该算法性能下降缓慢,丢包率和平均端到端延迟更低,且具有良好的负载均衡能力,有效增强了路由的可用性。

关键词：低轨卫星网络多路径路由链路属性卫星属性负载均衡

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Enhanced reversible hydrogen storage properties of wrinkled graphene microflowers confined LiBH_(4) system with high volumetric hydrogen storage capacity

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Materials Reports(Energy) 2024年第1期4卷 79-88页

作者： Zhenglong Li Kaicheng Xian Hao Chen Mingxia Gao Shanqing Qu meihong Wu Yaxiong Yang Wenping Sun Chao Gao Yongfeng Liu Xin Zhang Hongge Pan Institute of Science and Technology for New Energy Xi'an Technological UniversityXi'an710021PR China State Key Laboratory of Silicon and Advanced Semiconductor Materials Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang ProvinceSchool of Materials Science and EngineeringZhejiang UniversityHangzhou310027PR China Department of Polymer Science and Engineering MOE Key Laboratory of Macromolecular Synthesis and FunctionalizationZhejiang UniversityHangzhou310027PR China

LiBH_(4)with high hydrogen storage density,is regarded as one of the most promising hydrogen storage ***,it suffers from high dehydrogenation temperature and poor reversibility for practical *** is effective in achieving low dehydrogenation temperature and favorable ***,graphene can serve as supporting materials for LiBH_(4)catalysts and also destabilize LiBH_(4)via interfacial ***,graphene has never been used alone as a frame material for nanoconfining LiBH_(4).In this study,graphene microflowers with large pore volumes were prepared and used as nanoconfinement framework material for LiBH_(4),and the nanoconfinement effect of graphene was *** loading 70 wt%of LiBH_(4) and mechanically compressed at 350 MPa,8.0 wt% of H2 can be released within 100 min at 320C,corresponding to the highest volumetric hydrogen storage density of 94.9 g H2 L^(-1)ever *** to the nanoconfinement of graphene,the rate-limiting step of dehydrogenation of nanoconfined LiBH_(4) was changed and its apparent activation energy of the dehydrogenation(107.3 kJ mol^(-1))was 42%lower than that of pure LiBH_(4).Moreover,the formation of the intermediate Li_(2)B_(12)H_(12) was effectively inhibited,and the stable nanoconfined structure enhanced the reversibility of LiBH_(4).This work widens the understanding of graphene's nanoconfinement effect and provides new insights for developing high-density hydrogen storage materials.

关键词： Hydrogen storage LiBH_(4) Nanoconfinement Graphene High capacity

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免疫磁球性能影响因素

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食品与生物技术学报 2015年第5期34卷 501-506页

作者：杜美红代凤英许迪莘张淼孙永军左嘉北京市理化分析测试中心北京100089 中国科学院过程工程研究所北京100190 北京市科学器材公司北京100010

利用化学键法制备沙门氏菌捕获富集用免疫磁球,通过平板培养计数法确定其对目标菌的捕获率,对影响免疫磁球性能的磁分离时间、目标菌捕获率、特异性及敏感性等主要因素进行了研究。结果表明,免疫磁球在不同的磁分离时间内对目标菌的捕... 详细信息

利用化学键法制备沙门氏菌捕获富集用免疫磁球,通过平板培养计数法确定其对目标菌的捕获率,对影响免疫磁球性能的磁分离时间、目标菌捕获率、特异性及敏感性等主要因素进行了研究。结果表明,免疫磁球在不同的磁分离时间内对目标菌的捕获率不同,在一定的的磁分离时间内捕获率达到最高;磁球与抗体的投料比影响免疫磁球的捕获性能,磁球200μg、抗体25μg的投料制备的免疫磁球能获得40%以上的捕获效率,具有一定的灵敏性(100 cfu/m L的目标菌捕获率为50%以上);免疫磁球的粒径影响其捕获性能,大粒径(1 000 nm)免疫磁球目标菌捕获率小于小粒径(180 nm)免疫磁球目标菌捕获率;免疫磁球对单增李斯特菌、金黄色葡萄球菌、志贺氏菌非特异性在10%以下,在一定程度上影响免疫磁球的特异性捕获。

关键词：免疫磁球捕获率磁分离时间特异性敏感性

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