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Study on the Photosynthetic Characteristics of Six Varieties(Strains)in Chinese Chestnut

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Agricultural Biotechnology 2024年第1期13卷 9-14,57页

作者： lizhi FU yong YANG Xiaoxiao KANG Meng wang Jing LIU Xuan wang Dongsheng wang Liyang YU Hai’e zhang College of Horticultural Science and Technology Hebei Normal University of Science and TechnologyQinhuangdao 066004China Engineering Research Center of Chestnut Industrial Technology Ministry of EducationQinhuangdao 066004China Key Laboratory of Germplasm Mining and Innovative Utilization of Characteristic Horticulture Hebei ProvinceQinhuangdao 066004China Hebei Chestnut Industry Collaborative Innovation Center Qinhuangdao 066004China

[Objectives]This study was conducted to investigate the differences of photosynthetic physiological characteristics of different varieties(strains),which will provide a theoretical basis for high photosynthesis efficiency breeding and application in Chinese chestnut.[Methods]Six Chinese chestnut varieties of Castanea mollissima‘Yanbao’,***‘Yanqiu’,***‘Yanchang’,***‘Yanjia’,***‘Qianxi 37’,and ***‘Hybrid 22’were used as the *** the portable photosynthesis system Li-6400,we measured the photosynthetic characteristics and diurnal variation of leaf samples of six different chestnut varieties or *** fitted the light response curves and photosynthetic parameters using the leaf floating ***,we determined the chlorophyll content in the leaves using a UV-visible spectrophotometer.[Results]Among the six chestnut varieties or strains,‘Yanqiu’exhibited a significantly higher photosynthetic light saturation point(P LSP)compared to other five varieties,and‘Hybrid 22’ranked second,indicating that these two varieties had the strongest adaptation to high light *** photosynthetic light compensation point(P LCP)of‘Yanchang’was significantly higher than other five varieties,and"Qianxi 37"ranked second,indicating that these two varieties had the strongest adaptation to low light ***,they exhibited higher chlorophyll content and maintained good photosynthetic characteristics even in shaded environments with weak light ***‘Yanbao’and‘Yanjia’showed higher P LSP and lower P LCP,indicating that these two varieties have a wider range of adaptation to light *** were capable of efficiently utilizing light across a broader spectrum of intensities.‘Yanqiu’had the highest maximum net photosynthetic rate(P n,max)and the lowest dark respiration rate(R d),along with the highest chlorophyll *** indicated that‘Yanqiu’has strong photosynthetic capacity and organic mat

关键词： Chinese chestnut Diurnal variation of photosynthesis Light response model Light response curve Chlorophyll

Application of shallow high-resolution transient electromagnetic method( TEM) in geological investigation of debris flow

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Global Geology 2019年第2期22卷 91-97页

作者： LIU Chenyang zhang xiaopei du lizhi wang yong College of Construction and Engineering Jilin University

In the investigation of debris flow, the detection of the source area of the post-disaster debris flow is an important basis for evaluating the distribution of the debris flow accumulation layer and the subsequent control. In this paper, a shallow high-resolution TEM is used to detect the debris flow source area in Dashigou village, yongji County, Jilin Province. The purpose of this investigation is to determine the depth range of debris flow damage. The detection results show that there is an obvious low resistance zone at about 10 m depth along the survey line, which is in good agreement with the drilling data and the high density electrical detection. It is proved that the depth is the maximum impact depth of the debris flow. The practical engineering proves that the method has high resolution in shallow layer detection, high efficiency and convenience in field acquisition. The maximum detection depth range of this method is 30--40 m, which meets the requirements of high efficiency and accurate detection for regional debris flow source area, and has high practical application value.

关键词： shallow layer high resolution transient electromagnetic method debris flow

Application of TSD tunnel seismic prediction system in karst area

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Global Geology 2008年第4期11卷 246-250页

作者： lizhi du xiaopei zhang Jianjun NIU Xuege wang College of Construction Engineering Jilin University Changchun 130026 China China Northeast Municipal Engineering Design and Research Institute Changchun 130021 China

TSD is one of the classical methods of tunnel seismic prediction based on higher accuracy multi-wave multi-component *** working principle of the TSD and an application example of the TSD on tunnel prediction in Chongqing are introduced in this *** system has two ports for speed signal and acceleration signal,and the equipment is more portable and easy to *** to the application results we can conclude that the TSD prediction system is accurate and it has the wide application prospect in tunnel seismic detection.

关键词： TSD tunnel seismic prediction karst area

Application of ultrasonic CT method in nondestructive detection of interior defects in large scale concrete structural member of bridge

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Global Geology 2008年第4期11卷 218-221页

作者： xiaopei zhang Jianhui QIU Jianjun NIU lizhi du Xuege wang College of Construction Engineering Jilin university Changchun 130026 China China Northeast Municipal Engineering Design and Research Institute Changchun 130021 China China Northeast Municipal Engineering Design and Research Institute Changchun 130021 China

The ultrasonic computed tomography （USCT） method is derived from the basic principles of X-ray section scanning. This method records the arriving times of ultrasonic wave between the probes and the sources to ealculate the elastic wave velocity values in the section using the arrival times. Through analyzed the distribution Of elastic wave velocity in aim area, the information of the strength and the homogeneity of the investigated zone could be got indirectly. The authors introduced the operational principle of USCT and a practical case of using this method to detect the interior defects in large scale concrete structural member. Compared with other exploration methods, this method is more efficient and accurate.

关键词： Ultrasonic CT concrete structural member nondestructive detection

A failure criterion for shale considering the anisotropy and hydration based on the shear slide failure model

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International Journal of Mining Science and Technology 2023年第4期33卷 447-462页

作者： Qiangui zhang Bowei Yao Xiangyu Fan yong Li Nicholas Fantuzzi Tianshou Ma Yufei Chen Feitao Zeng Xing Li lizhi wang Petroleum Engineering School Southwest Petroleum UniversityChengdu 610500China Department of Civil Geological and Mining EngineeringÉcole Polytechnique de MontréalMontreal H3T 1J4Canada State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu 610500China School of Geoscience and Technology Southwest Petroleum UniversityChengdu 610500China State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400030China Department of Civil ChemicalEnvironmental and Materials EngineeringUniversity of BolognaBologna 40136Italy Department of Civil and Mineral Engineering University of TorontoToronto M5S 1A4Canada

A failure criterion fully considering the anisotropy and hydration of shale is essential for shale formation stability ***,a novel failure criterion for hydration shale is developed by using Jaeger’s shear failure criterion to describe the anisotropy and using the shear strength reduction caused by clay minerals hydration to evaluate the *** failure criterion is defined with four parameters in Jaeger’s shear failure criterion(S_(1),S_(2),a andφ),three hydration parameters(k,ω_(sh)andσ_(s))and two material size parameters(d and l0).The physical meanings and determining procedures of these parameters are *** accuracy and applicability of this failure criterion are examined using the published experimental data,showing a cohesive agreement between the predicted values and the testing results,R^(2)=0.916 and AAREP(average absolute relative error percentage)of 9.260%.The error(|D_(p)|)is then discussed considering the effects ofβ(angle between bedding plane versus axial loading),moisture content and confining pressure,presenting that|Dp|increases whenβis closer to 30°,and|D_(p)|decreases with decreasing moisture content and with increasing confining ***,|D_(p)|is demonstrated as being sensitive to S1and being steady with decrease in the data set whenβis 0°,30°,45°and 90°.

关键词： Shale Failure criterion Mechanical strength Shear slide failure Anisotropy Hydration

红色板栗和绿色板栗叶片光合特性及色素含量比较

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中国果树 2024年第6期 62-67页

作者：杨涌勾荣鑫付丽枝王萌王东升张海娥河北科技师范学院园艺科技学院秦皇岛066004 板栗产业技术教育部工程研究中心河北省特色园艺种质挖掘与创新利用重点实验室河北省板栗产业协同创新中心

以红色板栗红栗2号和绿色板栗燕宝为材料,通过Li-6400便携式光合仪测定叶片光合参数日变化以及红色板栗与绿色板栗的光响应曲线,同时使用紫外可见分光光度计测定叶片色素含量,探究红色板栗与绿色板栗叶片光合特性差异。结果表明:两种颜... 详细信息

以红色板栗红栗2号和绿色板栗燕宝为材料,通过Li-6400便携式光合仪测定叶片光合参数日变化以及红色板栗与绿色板栗的光响应曲线,同时使用紫外可见分光光度计测定叶片色素含量,探究红色板栗与绿色板栗叶片光合特性差异。结果表明:两种颜色板栗净光合速率日变化有明显区别,绿色板栗净光合速率为双峰形曲线,13:00时出现“光合午休”现象,而红色板栗为单峰形,无明显“光合午休”现象;红色板栗气孔导度、蒸腾速率、水分利用率日均值均大于绿色板栗,而胞间二氧化碳浓度日均值小于绿色板栗。光响应曲线结果表明,红色板栗有更高的最大净光合速率、光补偿点、暗呼吸速率、光饱和点与表观量子效率,与绿色板栗差异显著。对叶片色素含量进行分析,红色板栗与绿色板栗类胡萝卜素含量无明显差异,但是叶绿素含量和花色素苷含量差异显著。相关性分析结果表明,光响应特征参数中最大净光合速率、光饱和点与花色素苷含量有极显著正相关性,而光补偿点、暗呼吸速率与叶绿素含量呈现极显著或显著负相关。

关键词：板栗光合日变化光响应曲线花色素苷叶绿素

The seismic CT method in measuring rock bodies

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Applied Geophysics 2006年第3期3卷 192-195页

作者： du lizhi zhang xiaopei Niu Jianjun wang Xuege Feng Guolei College of Construction Engineering Jilin University Changchun 130026 China College of Resource and Environment Jilin University Changchun 130026 China

The seismic computed tomography （CT） method is derived from the basic principles of X-ray section scanning first applied in medical science. The method records P-wave arrivals between shots and receivers in separate boreholes. Using the velocity information from 2D seismic P-wave arrival inversion, we can determine the distribution of velocity in rock and soil bodies. This paper introduces a practical case of using the seismic CT method for detecting the structure of the rocks for tunnel engineering and to utilize SIRT algorithms for doing first arrival time iterative inversion. Compared with other exploration methods, it is more efficient and accurate.

关键词： seismic computed tomography P-wave velocity travel time inversion.

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高灵敏度感应式磁传感器测试研究

地球物理学报 2019年第10期62卷 3760-3771页

作者：王勇佴磊牛建军张晓培杜立志吉林大学建设工程学院

本文着重对研制的感应式磁传感器的本底噪声和灵敏度特性进行测试研究,在平行噪声测试方法(NPI)基础上设计了针对本研究的磁传感器噪声测试方法,测试结果表明:MC-30的噪声水平1Hz时为0.3pT/Hz,10Hz时为0.03pT/Hz,100Hz时为10fT/Hz,1kHz... 详细信息

本文着重对研制的感应式磁传感器的本底噪声和灵敏度特性进行测试研究,在平行噪声测试方法(NPI)基础上设计了针对本研究的磁传感器噪声测试方法,测试结果表明:MC-30的噪声水平1Hz时为0.3pT/Hz,10Hz时为0.03pT/Hz,100Hz时为10fT/Hz,1kHz时为3fT/Hz,灵敏度为215mV/nT;MC-50的噪声水平0.01Hz时为10pT/Hz,1Hz时为0.05pT/Hz,10Hz时为10fT/Hz,100Hz时为0.5fT/Hz,灵敏度为1359mV/nT.除此之外,通过野外试验对比及工程应用验证了MC-30和MC-50具备良好的测量精度及野外工作性能,能够满足实际电磁勘探任务的需要.

关键词：感应式磁场传感器噪声灵敏度测试

Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG

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Molecular Plant 2019年第7期12卷 1027-1036页

作者： Zhaohui Zhong Simon Sretenovic Qiurong Ren Lijia Yang Yu Bao Caiyan Qi Mingzhu Yuan Yao He Shishi Liu xiaopei Liu Jiaheng wang Lan Huang Yan wang Dibin Baby David wang Tao zhang Yiping Qi yong zhang Department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of 曰ectronic Science and Technology of China Room 216 Main Building No. 4 Section 2 North Jianshe Road Chengdu 610054 China Department of Plant Science and Landscape Architecture University of Maryland College Park MD 20742 USA Jiangsu Key Laboratory of Crop Genetics and Physiology Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of Education Yangzhou University Yangzhou 225009 China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou University Yangzhou 225009 China Indian Institute of Science Education and Research Timpati Andhra Pradesh 517507 India Seven Lakes High School Katy TX 77494 USA Institute for Bioscience and Biotechnology Research University of Maryland Rockville MD 20850 USA

Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising potential in improving targeting specificity and broadening the targeting range. In this study, we evaluated these Cas9 variants in the model and crop plant, rice. We first tested xCas9-3.7, the most effective xCas9 variant in mammalian cells, for targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates. xCas9 exhibited nearly equivalent editing efficiency to wild-type Cas9 (Cas9-WT) at most canonical NGG PAM sites tested, whereas it showed limited activity at non-canonical NGH (H = A, C, T) PAM sites. High editing efficiency of xCas9 at NGG PAMs was further demonstrated with C to T base editing by both rAPOBECI and PmCDAI cytidine deaminases. With mismatched sgRNAs, we found that xCas9 had improved targeting specificity over the Cas9-WT. Furthermore, we tested two Cas9-NG variants, Cas9-NGv1 and Cas9-NG, for targeting NGN PAMs. Both Cas9-NG variants showed higher editing efficiency at most non-canonical NG PAM sites tested, and enabled much more efficient editing than xCas9 at AT-rich PAM sites such as GAT, GAA, and CAA. Nevertheless, we found that Cas9-NG variants showed significant reduced activity at the canonical NGG PAM sites. In stable transgenic rice lines, we demonstrated that Cas9-NG had much higher editing efficiency than Cas9-NGv1 and xCas9 at NG PAM sites. To expand the base-editing scope, we developed an efficient C to T base-editing system by making fusion of Cas9-NG nickase (D10A version), PmCDAI, and UGI. Taken together, our work benchmarked xCas9 as a high-fidelity nuclease for targeting canonical NGG PAMs and Cas9-NG as a preferred variant for targeting relaxed PAMs for plant genome editing.

关键词： xCas9 Cas9-NG high fidelity non-canonical PAM base editing