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Clathrin light chains negatively regulate plant immunity by hijacking the autophagy pathway

Plant Communications 2024年第8期5卷 172-188页

作者： Hu-Jiao Lan Jie Ran Wen-Xu Wang Lei Zhang Ni-Ni Wu Ya-Ting Zhao Min-Jun Huang Min Ni fen Liu Ninghui Cheng Paul A.Nakata jianwei pan Steven A.Whitham Barbara J.Baker jian-Zhong Liu Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants Zhejiang Normal UniversityJinhua 321004China Institute of Plant Genetics and Developmental Biology College of Chemistry and Life SciencesZhejiang Normal UniversityJinhua 321004China College of Life Sciences Lanzhou UniversityLanzhou 730000China Department of Plant Pathology and Microbiology Iowa State UniversityAmesIA 50011USA Department of Plant and Microbial Biology University of CaliforniaBerkeleyBerkeleyCA 94720USA Plant Gene Expression Center U.S.Department of Agriculture-Agricultural Research ServiceAlbanyCA 94706USA Lushan Botanical Garden Chinese Academy of SciencesJiujiang 332000China U.S.Department of Agriculture-Agricultural Research Service Children’s Nutrition Research CenterDepartment of PediatricsBaylor College of MedicineHoustonTX 77030USA

The crosstalk between clathrin-mediated endocytosis(CME)and the autophagy pathway has been reported in mammals;however,the interconnection of CME with autophagy has not been established in ***,we report that the Arabidopsis CLATHRIN LIGHT CHAIN(CLC)subunit 2 and 3 double mutant,clc2-1 clc3-1,phenocopies Arabidopsis AUTOPHAGY-RELATED GENE(ATG)mutants in both autoimmunity and nutrient ***,the autophagy pathway is significantly compromised in the clc2-1 clc3-1 ***,multiple assays demonstrate that CLC2 directly interacts with ATG8h/ATG8i in a domain-specific *** expected,both GFP-ATG8h/GFP-ATG8i and CLC2-GFP are subjected to autophagic degradation,and degradation of GFP-ATG8h is significantly reduced in the clc2-1 clc3-1 ***,simultaneous knockout of ATG8h and ATG8i by CRISPR-Cas9 results in enhanced resistance against Golovinomyces cichoracearum,supporting the functional relevance of the CLC2-ATG8h/8i *** conclusion,our results reveal a link between the function of CLCs and the autophagy pathway in Arabidopsis.

关键词： autophagy clathrin light chain immunity clathrin-mediated endocytosis senescence cell death

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广州西村窑与海上丝绸之路

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岭南文史 2024年第2期 54-60页

作者：潘剑芬梁东邓世昌纪念馆(广州市海珠博物馆) 不详

广州西村窑兴盛于北宋。宋代的广州是对外贸易的重要口岸,西村窑在生产技艺上采各名窑之长,产品以质朴、价廉的优势大量销往东南亚各国。但因其市场和产品定位没有随着时代变迁做出及时调整、工艺缺乏提高和创新、原材料供应失去优势,... 详细信息

广州西村窑兴盛于北宋。宋代的广州是对外贸易的重要口岸,西村窑在生产技艺上采各名窑之长,产品以质朴、价廉的优势大量销往东南亚各国。但因其市场和产品定位没有随着时代变迁做出及时调整、工艺缺乏提高和创新、原材料供应失去优势,以及失去特殊政策的支持、对外贸易的重心转移等多方面因素影响,导致南宋后西村窑逐步走向衰落。基于西村窑的历史贡献,传承、优化、重振西村窑,对广州推进“一带一路”建设和申报“海上丝绸之路”世界文化遗产,具有重要的现实价值和意义。

关键词：广州西村窑制瓷技艺海上丝绸之路

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2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-Glucoside modulates CHEK2 and CCND1 alternative splicing to inhibit MCF-7 cells proliferation

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Traditional Medicine Research 2024年第1期9卷 33-46页

作者： Hui Shen You-Zhi Zhang Peng-Yu Wang Shuo Zhang Huan pan Bei-Bei Liu Long-Sheng Xu jian-fen Shen Department of Central Laboratory The First Affiliated Hospital of Jiaxing UniversityJiaxing 314000China School of Pharmacy Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Hubei Engineering Research Center of Traditional Chinese Medicine of South Hubei Province Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China

Background:In our previous study,we observed a synergistic effect of 2,3,5,4’-Tetrahydroxystilbene-2-O-b-D-glucoside combined with adriamycin to induce apoptosis in MCF-7 breast cancer ***,the underlying mechanisms of epigenetic modifications,such as alternative splicing,have not been *** this study,we aimed to investigate the mechanism by which THSG inhibits MCF-7 cell proliferation using full-length transcriptome ***:First,cell viability was examined using the methyl thiazolyl tetrazolium method and full-length transcriptome sequencing was performed to identify genes and *** Ontology and Kyoto Encyclopedia of Genes and Genomes were used to identify the principal pathways and targets of *** cytometry analysis of cell cycle distribution was ***,the analysis of alternative splicing and domains of the key proteins was *** polymerase chain reaction and western blotting were performed for ***:THSG showed significant cytotoxic activity in MCF-7 ***-length transcriptome sequencing revealed differential alternative splicing with 173 upregulated and 263 downregulated *** analysis identified distinct differential expression of genes(CHEK2-211 and CCND1-201)involved in the cell cycle in the THSG-treated ***,alternative splicing types of CHEK2(mutually exclusive exon)and CCND1(intron retention).We found that THSG downregulated mRNA expression,as confirmed by quantitative polymerase chain reaction ***,protein structural analysis revealed that THSG treatment led to the generation of CHK2-211,which was the result of a mutation in the amino acid residues(GLU-150,ASN-151)of the CHEK2 domain(VAL-150,GLY-151).and CyclinD1-201 were obtained when an amino acid(ASP-267)in the domain was lost in ***,molecular docking analysis demonstrated that the domains of key proteins could bind THSG more effectively,with no difference in

关键词： THSG breast cancer full-length transcriptome sequencing alternative splicing

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“刚”“柔”并济的新型技术创新风控模式的构建——基于中科金财的探索性案例研究

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管理案例研究与评论 2024年第2期17卷 173-187页

作者：吕晨王健李莉李芬芳潘辉天津城建大学经济与管理学院天津300384 南开大学商学院天津300071

快速变化的环境加剧了科技型企业的技术创新风险,企业需要构建兼具快变“弹性”和管控“刚性”的新型技术创新风控模式。将不完全契约理论嵌入传统风控理论,通过对中科金财独特的风控模式展开情境化研究,解锁了科技型企业构建“刚”“... 详细信息

快速变化的环境加剧了科技型企业的技术创新风险,企业需要构建兼具快变“弹性”和管控“刚性”的新型技术创新风控模式。将不完全契约理论嵌入传统风控理论,通过对中科金财独特的风控模式展开情境化研究,解锁了科技型企业构建“刚”“柔”并济风控模式的过程。具体而言,首先,企业应下沉风控单元,形成“虚拟小微企业”,加快反应速度,获取风控“弹性”,同时通过业绩认证保持必要的财务“刚性”;其次,构建“多层联盟”的风控结构,一方面通过动态调整增加结构“弹性”,另一方面则通过新规则稳固内部关系,既保证管控“刚性”,又实现对创新失败的容错“弹性”;最后,构建了“虚拟小微企业+多层联盟”的新型技术创新风控模式。基于技术创新的本质特征发展了风控理论,同时对不完全契约理论做出了一定的贡献。

关键词：技术创新风控模式不完全契约理论容错信号

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基于激光点云的盆景可视化建模方法研究

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地矿测绘 2024年第3期40卷 47-50页

作者：张华葛亚建吕敏潘坚英王雪芬刘宇浙江省测绘科学技术研究院浙江杭州311100 浙江省国土勘测规划有限公司浙江杭州310030 杭州西湖风景名胜区花港管理处浙江杭州310013

盆景作为中华传统艺术的优秀代表之一,具有很强的观赏性,但定期修剪和整形均潜藏着不可逆的隐患,因此诞生了虚拟盆景整形修剪的概念,而盆景可视化模型的重建则是实现虚拟修剪的重要前提。为此,利用手持式三维激光扫描仪获取盆景点云数据... 详细信息

盆景作为中华传统艺术的优秀代表之一,具有很强的观赏性,但定期修剪和整形均潜藏着不可逆的隐患,因此诞生了虚拟盆景整形修剪的概念,而盆景可视化模型的重建则是实现虚拟修剪的重要前提。为此,利用手持式三维激光扫描仪获取盆景点云数据,并依靠点云的邻域点分布特征完成枝叶点判断,最终成功构建出盆景的可视化模型,为后续盆景虚拟修剪提供了数据基础。

关键词：点云盆景三维激光扫描技术可视化模型

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中风醒脑液通过抑制神经元铁死亡发挥抗脑出血损伤的作用机制研究

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中国中药杂志 2024年第17期49卷 4723-4733页

作者：李孟英郭建文邵翀羽泮智勇陈天杭周科峰周惠芬万海同浙江中医药大学第一附属医院浙江杭州310006 浙江中医药大学生命科学学院浙江杭州310053 浙江中医药大学基础医学院浙江杭州310053 全省中医心脑血管病重点实验室浙江杭州310053 广州中医药大学第二附属医院省部共建中医湿证国家重点实验室广东广州510120 广州中医药大学第二附属医院脑病中心广东广州510120

中风醒脑液在临床上已被用于治疗脑出血。然而,目前对中风醒脑液的药理作用尚不明确。探讨中风醒脑液的药理作用对指导脑血管疾病的治疗至关重要。向大鼠右侧尾状核注射Ⅶ型胶原酶建立体内脑出血模型。将SD大鼠随机分成5组,用Bederson... 详细信息

中风醒脑液在临床上已被用于治疗脑出血。然而,目前对中风醒脑液的药理作用尚不明确。探讨中风醒脑液的药理作用对指导脑血管疾病的治疗至关重要。向大鼠右侧尾状核注射Ⅶ型胶原酶建立体内脑出血模型。将SD大鼠随机分成5组,用Bederson评分法评估大鼠术后的神经功能缺损情况。核磁共振成像(MRI)用于评估脑出血的体积。苏木精-伊红(HE)染色观察脑组织病理改变,透射电镜(TEM)观察脑组织超微结构变化。流式细胞术测定活性氧(ROS)水平。免疫荧光双染法检测脑组织血肿周围神经元中铁转运蛋白标记物谷胱甘肽过氧化物酶4(GPX4)的表达。Western blot法检测脑出血后RNA解旋酶和ATP酶1(UPF1)、铁泵蛋白(FPN)、酰基辅酶A连接酶4(ACSL4)、环氧化酶2(COX2)、GPX4、NADPH氧化酶1(NOX1)和胱氨酸/谷氨酸逆向转运蛋白(SLC7A11)的表达。与模型组相比,中风醒脑液治疗5 d后大鼠神经功能障碍明显减轻,血肿体积减小,脑出血病理损伤减轻;与此同时,Fe^(2+)和氧化应激水平显著降低,抑制铁死亡的蛋白表达量显著增加。综上,中风醒脑液可通过显著抑制神经元铁死亡改善大鼠脑出血的预后,为中风醒脑液的临床应用提供了有价值的指导。中风醒脑液的抗铁死亡作用可能与促进膜转运蛋白SLC7A11和FPN的表达有关。

关键词：脑出血铁死亡中风醒脑液神经元死亡

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Risk Assessment of MOAH and MOSH in Infants and Young Children

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Biomedical and Environmental Sciences 2019年第2期32卷 130-133页

作者： ZHU Lei ZHANG Hong CHEN Yan fen pan Jing Jing LIU Ai Dong pan feng ZHANG jian Bo ZHONG Huai Ning China National Centre for Food Safety Risk Assessment Guangdong Inspection and Quarantine Technology Centre

Mineral oil hydrocarbons(MOH) in food may come from both contamination and various intentional uses in food production. MOH is widely used in food contact materials and food additives. It comprises complex mixtures, i... 详细信息

关键词： In Risk Assessment Young Children

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Comparative genomic study of gastric epithelial cells co-cultured with Helicobacter pylori

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World Journal of Gastroenterology 2012年第48期18卷 7212-7224页

作者： fen Wang Li-Dan Luo jian-Hua pan Li-Hua Huang Hong-Wei Lv Qin Guo Can-Xia Xu Shou-Rong Shen Department of Gastroenterology The Third Xiangya HospitalCentral South University Department of Gastroenterology Hunan Provincial Hospital

AIM:To identify genes potentially involved in Helicobacter pylori(***)-induced gastric ***:GES-1 cells were co-cultured with *** strains isolated from patients with gastric carcinoma(GC,n = 10) or chronic gastritis(CG,n = 10) for in vitro proliferation and apoptosis assays to identify the most and least virulent *** two strains were cagA-genotyped and used for further in vivo carcinogenic virulence assays by infecting Mongolian gerbils for 52 wk,respectively;a broth free of *** was lavaged as *** profiles of GES-1 cells cocultured with the most and least virulent strains were determined by microarray *** most differentially expressed genes were further verified using quantitative real-time polymerase chain reaction in GES-1cells infected with the most and least virulent strains,and by immunohistochemistry in *** positive CG,precancerous diseases,and GC biopsy specimens in an independent ***:GC-derived *** strains induced a potent proliferative effect in GES-1 cells in co-culture,whereas CG-derived strains did *** most(from a GC patient) and least(from a CG patient) virulent strains were cagA-positive and negative,*** week 52,CG,atrophy,metaplasia,dysplasia,and GC were observed in 90.0%,80.0%,80.0%,90%,and 60.0%,respectively,of the animals lavaged with the most virulent ***,only mild CG was observed in 90% of the animals lavaged with the least virulent *** microarray analysis,800 differentially expressed genes(49 up-and 751 down-regulated),involving those associated with cell cycle regulation,cell apoptosis,cytoskeleton,immune response,and substance and energy metabolisms,were identified in cells co-cultured with the most virulent strain as compared with those co-cultured with the least virulent *** six most differentially expressed genes(with a betweenness centrality of 0.1-0.2) were identified among the significant differential gene prof

关键词： Helicobacter pylori Gastric carcinoma Proliferation Genomic profiles

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Plasma exchange-centered artificial liver support system in hepatitis B virus-related acute-onchronic liver failure:a nationwide prospective multicenter study in China

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Hepatobiliary & pancreatic Diseases International 2016年第3期15卷 275-281页

作者： Jia-Jia Chen jian-Rong Huang Qian Yang Xiao-Wei Xu Xiao-Li Liu Shao-Rui Hao Hui-fen Wang Tao Han Jing Zhang jian-He Gan Zhi-Liang Gao Yu-Ming Wang Shu-Mei Lin Qing Xie Chen pan Lan-Juan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases and Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesFirst Affiliated HospitalZhejiang University School of Medicine Liver Failure Therapy and Research Center 302 Hospital of People’s Liberation Army Department of Hepatology Third Central Clinical College of Tianjin Medical University Artificial Liver Center You’an HospitalCapital Medical University Department of Infectious Diseases First Affiliated Hospital of Suzhou University Department of Infectious Diseases Third Affiliated Hospital of Sun Yat-Sen University Institute of Infectious Diseases Southwest HospitalThird Military Medical University Department of Infectious Diseases First Affiliated Hospital of Xi’an Jiaotong University Department of Infectious Diseases Ruijin HospitalShanghai Jiaotong University School of Medicine Department of Infectious Diseases Infectious Diseases Hospital Affiliated to Fujian Medical University

BACKGROunD： Plasma exchange （PE）-centered artificial liver support system reduced the high mortality rate of hepa titis B virus （HBV）-related acute-on-chronic liver failure （ACLF）. But the data were diverse in different medical centers. The present prospective nationwide study was to evaluate the effects of PE on patients with HBV-ACLF at different stages.

关键词： liver failure artificial liver support plasma exchange acute-on-chronic liver failure

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多维目标单元教学设计和实施——以篮球体前变向换手运球为例

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体育学刊 2019年第3期26卷 115-120页

作者：潘建芬北京教育学院体育与艺术教育学院

为理解多维目标单元教学设计和实施,以篮球体前变向换手运球单元教学为例进行分析。研究表明:体育课程使命赋予多维目标单元教学的可能性,多维目标单元教学符合运动习得规律,能够把学生作为一个有健全人格的完整之人来培养;常态篮球课... 详细信息

为理解多维目标单元教学设计和实施,以篮球体前变向换手运球单元教学为例进行分析。研究表明:体育课程使命赋予多维目标单元教学的可能性,多维目标单元教学符合运动习得规律,能够把学生作为一个有健全人格的完整之人来培养;常态篮球课教学缺乏技术之上的目标设计,缺乏对学生的关照,主要体现为缺乏篮球教学设计的多维行动目标;篮球体前变向换手运球蕴含“认知-技能-品德”的多维目标,要有重点、有机兼顾、螺旋式上升地设计进阶目标;依据组合技术要领、体育中考需要和基本技术,进行篮球体前变向换手运球单元教学重难点目标确定及进阶安排。

关键词：体育教学多维目标单元教学设计与实施篮球体前变向换手运球

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