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Genomic analysis of a pure culture of magnetotactic bacterium Terasakiella ***-1

Journal of Oceanology and Limnology 2021年第6期39卷 2097-2106页

作者： haijian du Wenyan ZHANG Wei LIN Hongmiao PAN Tian XIAO Long-Fei WU College of Life Science Shandong UniversityQingdao 266237China CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China Key Laboratory of Earth and Planetary Physics Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Institutions of Earth Science Chinese Academy of SciencesBeijing 100029China France-China Joint Laboratory for Evolution and Development of Magnetotactic Multicellular Organisms Chinese Academy of SciencesBeijing 100029China Aix-Marseille University CNRSLCBMarseille F-13402France

Magnetotactic bacteria(MTB)display magnetotaxis ability because of biomineralization of intracellular nanometer-sized,membrane-bound organelles termed *** having been discovered more than half a century,only a few representatives of MTB have been isolated and cultured in the *** this study,we report the genomic characterization of a novel marine magnetotactic spirillum strain SH-1 belonging to the genus Terasakiella that was recently isolated.A gene encoding haloalkane dehalogenase,which is involved in the degradation of chlorocyclohexane,chlorobenzene,chloroalkane,and chloroalkene,was ***-1 genome contained cysCHI and soxBAZYX genes,thus potentially capable of assimilatory sulfate reduction to H_(2)S and using thiosulfate as electron donors and oxidizing it to *** of SH-1 also contained genes encoding periplasmic dissimilatory nitrate reductases(napAB),assimilatory nitrate reductase(nasA)and assimilatory nitrite reductases(nasB),suggesting that it is capable of gaining energy by converting nitrate to *** pure culture of Terasakiella ***-1 together with its genomic results off ers new opportunities to examine biology,physiology,and biomineralization mechanisms of MTB.

关键词： magnetotactic bacteria magnetotaxis pure culture comparative genomic analysis

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Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China

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Acta Oceanologica Sinica 2017年第2期36卷 94-104页

作者： du haijian ZHANG Rui ZHANG Wenyan XU Cong CHEN Yiran PAN Hongmiao ZHOU Ke WU Long-fei XIAO Tian Key Laboratory for Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China College of Resources and Environment Qingdao Agriculture University Qingdao 266109 China Aix Marseille Université CNRS LCB UMR 7257 Institut de Microbiologie de la Méditerranée Marseille F-13402 France University of Chinese Academy of Sciences Beijing 100049 China

Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake（China）, where their abundance reached 103–104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy（HRTEM） analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16 S r RNA gene sequence analysis, we found fourteen operational taxonomic units（OTUs） which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the *** with known axenic and uncultured marine magnetotactic bacteria, the 16 S r RNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to96.2%（〈97%）. The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.

关键词： magnetotactic bacteria magnetosome biodiversity Yuehu Lake intertidal sediments

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电动汽车新型超级电容能量管理系统设计

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电子技术应用 2011年第6期37卷 77-80页

作者：杜海健苏谢祖颜钢锋浙江大学电气工程学院浙江杭州310027

设计了一种应用于电动汽车的新型能量管理系统,分析了基于超级电容的双向DC-DC变换器原理。在分析电动汽车运行特性的基础上设计了该能量管理系统的控制策略。系统在电机控制部分采用相电流闭环控制,在双向DC-DC变换器部分采用电压、电... 详细信息

设计了一种应用于电动汽车的新型能量管理系统,分析了基于超级电容的双向DC-DC变换器原理。在分析电动汽车运行特性的基础上设计了该能量管理系统的控制策略。系统在电机控制部分采用相电流闭环控制,在双向DC-DC变换器部分采用电压、电流双闭环控制。

关键词：能量回馈控制策略双向DC—DC UCC3803A 双闭环

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Efficient fixation of CO_(2) to cyclic carbonates and oxazolidinones with multi-hydroxyl bis-(quaternary ammonium) ionic liquids as catalysts under mild conditions

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中南民族大学学报（自然科学版） 2025年第1期44卷 1-8页

作者： BAI Yumeng YANG haijian School of Environmental Engineering and Chemistry Luoyang Institute of Science and TechnologyLuoyang 471023Henan China College of Chemistry and Materials Science South-Central Minzu UniversityWuhan 430074China

A series of multi-hydroxyl bis-(quaternary ammonium)ionic liquids(Ils1‒7)was prepared as bifunctional catalysts for the chemical fixation of CO_(2).All these ionic liquid compounds were efficient for the catalytic synthesis of cyclic carbonates and oxazolidinones via the cycloaddition reactions between CO_(2) and epoxides or aziridines with excellent yield and high selectivity in the absence of co-catalyst,metal and *** to the synergistic effects of hydroxyl groups and halogen anion,the cycloaddition reactions proceeded smoothly either at atmospheric pressure or room *** selectivity for substituted oxazolidinones at 5-and 4-positions can be tuned via changing the reaction ***,possible mechanisms including the activation of both CO_(2) and epoxide or aziridines were proposed based on the literatures and experimental results.

关键词： chemical conversion of CO_(2) multi-hydroxyl bis-(quaternary ammonium) ionic liquids

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经验模态分解法在地震资料处理中的应用试验

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地震地磁观测与研究 2012年第2期33卷 40-45页

作者：梁亮王蓓杜海舰上海采油技术服务分公司中国上海200030

通过对多种连续小波基的平稳序列时频分析,提出经验模态的非平稳序列的时频分析方法,并在地震资料处理解释应用中作探索性试验。试验结果表明:对地震记录道长为5 s和2 ms采样资料,经验模态分解后可获得6—7个固有模态函数单分量,分解的... 详细信息

通过对多种连续小波基的平稳序列时频分析,提出经验模态的非平稳序列的时频分析方法,并在地震资料处理解释应用中作探索性试验。试验结果表明:对地震记录道长为5 s和2 ms采样资料,经验模态分解后可获得6—7个固有模态函数单分量,分解的第一个分量C1(t)具有较宽的频率成分,可作为地震属性的主分量。

关键词：非平稳序列时频分析经验模态分解固有模态函数地震资料

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吉非替尼下调肿瘤抑制素M抑制博莱霉素诱导的小鼠肺纤维化

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免疫学杂志 2016年第4期32卷 305-309页

作者：郑林鑫郑林鑫麦玉梅杜海坚李理李伟峰南方医科大学研究生学院 510515 广州军区广州总医院呼吸内科 510010

目的研究肿瘤抑制素M(OSM)及其下游通路在吉非替尼干预的肺纤维化小鼠中的改变,探讨OSM及下游通路在肺纤维化中的作用。方法 36只SPF级昆明小鼠随机分为3组:正常组(生理盐水气管内雾化)、博莱霉素组(博莱霉素3 mg/kg气管内雾化)、吉非... 详细信息

目的研究肿瘤抑制素M(OSM)及其下游通路在吉非替尼干预的肺纤维化小鼠中的改变,探讨OSM及下游通路在肺纤维化中的作用。方法 36只SPF级昆明小鼠随机分为3组:正常组(生理盐水气管内雾化)、博莱霉素组(博莱霉素3 mg/kg气管内雾化)、吉非替尼处理组(博莱霉素气管内雾化后,每天吉非替尼20 mg/kg灌胃)。实验第14天收集肺标本,肺组织行HE与Masson染色;RT-PCR法检测α-SMA、OSM m RNA表达水平;Western blot法检测α-SMA、OSM、ERK1/2、P-ERK1/2、P38、P-P38蛋白表达水平。结果博莱霉素组小鼠肺组织病理损伤较正常组小鼠明显加重、胶原沉积明显增加、炎症损伤评分及纤维化评分明显增加(P<0.05),α-SMA、OSM m RNA及蛋白表达水平明显升高(P<0.05),p-ERK1/2、p-P38蛋白表达水平明显升高(P<0.05),吉非替尼组小鼠上述指标均较博莱霉素组明显降低(P<0.05)。结论吉非替尼能显著缓解博莱霉素诱导的小鼠肺纤维化,其机制可能与抑制OSM m RNA及蛋白的表达及其下游P38和ERK1/2的磷酸化密切相关。

关键词：吉非替尼肿瘤抑制素M(OSM) 博莱霉素肺纤维化

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特发性肺间质纤维化的药物治疗研究及新进展

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国际呼吸杂志 2016年第1期36卷 69-73页

作者：郑林鑫杜海坚李伟峰广州军区广州总医院呼吸内科 510010

特发性肺间质纤维化（IPF）发病率高，药物治疗手段非常有限，预后不良。近10年来国内外学者开展了许多有关IPF药物治疗的临床研究，不断推动着IPF治疗指南的发展。现对近10年来有关IPF药物治疗的大型临床研究作一综述，介绍其研究成果... 详细信息

特发性肺间质纤维化（IPF）发病率高，药物治疗手段非常有限，预后不良。近10年来国内外学者开展了许多有关IPF药物治疗的临床研究，不断推动着IPF治疗指南的发展。现对近10年来有关IPF药物治疗的大型临床研究作一综述，介绍其研究成果及对未来的展望。

关键词：特发性肺间质纤维化药物治疗临床研究

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The Research on the Relationship between Corporate Social Responsibility and Common Prosperity

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Frontiers of Business Research in China 2022年第4期16卷 371-393页

作者： Li haijian du Shuang Institute of Quantitative&Techno-logical Economics Chinese Academy of Social SciencesBeijing 100732China China Laboratory of Economic and Social Development and Intelligent Governance Beijing 100732China

Corporate social responsibility(CSR)and common prosperity have the consistency of philosophy and *** the process of delivering CSR,enterprises can integrate their tangible and intangible assets to realize a positive cycle of sustainable development and common *** can also open up space for both commercial and social value by integrating altruism with self-interest,public welfare with utilitarianism,cost with capital,and today’s development with tomorrow’s ***,the unification of commercial value and social value can extend pathways to common prosperity and improve common *** way of the super CSR,enterprises can spread the wealth of their culture and values to society through material products,ethical products,and intergenerational inheritance based on the high compatibility of enterprise high-dimensional culture,enterprise craftsmanship,and enterprise *** can thus play a leading role in promoting the ethical wealth of the whole of *** and private enterprises should maximize their respective advantages in promoting common prosperity of society for realizing the common progress of the people,and should strike a balance between efficiency and fairness.

关键词： corporate social responsibility(CSR) commercial value social value common prosperity

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Nucleation mechanisms of dynamic recrystallization in Inconel 625 superalloy deformed with different strain rates

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Rare Metals 2012年第3期31卷 215-220页

作者： Guo, Qingmiao Li, Defu Peng, haijian Guo, Shengli Hu, Jie du, Peng Nonferrous Metals Processing Division General Research Institute for Nonferrous Metals Beijing 100088 China

The effects of strain rates on the hot working characteristics and nucleation mechanisms of dynamic recrystallization (DRX) were studied by optical microscopy and electron backscatter diffraction (EBSD) technique. Hot compression tests were conducted using a Gleeble-1500 simulator at a true strain of 0.7 in the temperature range of 1000 to 1150 °C and strain rate range of 0.01 to 10.00 s?1. It is found that the size and volume fraction of the DRX grains in hot-deformed Inconel 625 superalloy firstly decrease and then increase with increasing strain rate. Meanwhile, the nucleation mechanism of DRX is closely related to the deformation strain rate due to the deformation thermal effect. The discontinuous DRX (DDRX) with bulging of original grain boundaries is the primary nucleation mechanism of DRX, while the continuous DRX (CDRX) with progressive subgrain rotation acts as a secondary nucleation mechanism. The twinning formation can activate the nucleation of DRX. The effects of bulging of original grain boundaries and twinning formation are firstly gradually weakened and then strengthened with the increasing strain rate due to the deformation thermal effect. On the contrary, the effect of subgrain rotation is firstly gradually strengthened and then weakened with the increasing strain rate.

关键词： nucleation mechanisms dynamic recrystallization Inconel 625 superalloy deformation strain rate

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Fracture evolution characteristics of sandstone containing double fissures and a single circular hole under uniaxial compression

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International Journal of Mining Science and Technology 2017年第3期27卷 499-505页

作者： Chen Minliang Jing Hongwen Ma Xiujun Su haijian du Mingrui Zhu Tantan State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining & Technology Xuzhou 221116 China Tianjin Traffic Science Research Institute Tianjin 300074 China JSTI GROUP Nanfing 210000 China

The uniaxial compression experiments on the sandstone samples containing double fissures and a single circular hole were carried out by using electro-hydraulic servo universal testing machine to investigate the effect of rock bridge angle β and fissure angle α on mechanical properties and evolution characteristics of *** results show that the peak strength,peak strain and elastic modulus of defected specimens decrease comparing with those for intact sample,and show a decreased trend firstly and then increase with β changing from 0° to 90°.The peak strength and elastic modulus achieve the minimum value as the rock bridge angle is 60°,while the peak strain reaches the minimum value with the rock bridge angle of 45°.The crack initiation of tested rock samples occurs firstly in stress concentration areas at tips of prefabricated fissures under uniaxial compression,and then propagates constantly and coalescences with the prefabricated *** secondary cracks initiate and propagate as well until buckling failure *** rock bridge angle has a great influence on crack initiation,coalescence,final failure mode,crack initiation stress and transfixion *** peak strength varies significantly,while the elastic modulus and peak strain change slightly,and the failure modes are also different due to the influence of fissure angle.

关键词： Double fissures A single circular hole Strength characteristics Crack propagation Failure mode

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