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Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism

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Journal of Pharmaceutical Analysis 2023年第12期13卷 1562-1576页

作者： Jiajun Chen Tian Li Dehua Huang Wenxia Gong Junsheng Tian Xiaoxia Gao Xuemei Qin guanhua du Yuzhi Zhou Modern Research Center for Traditional Chinese Medicine Shanxi UniversityTaiyuan030006China The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education Shanxi UniversityTaiyuan030006China The Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province Taiyuan030006China Institute of Materia Medica Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100050China

Chaigui granules(CG)are a compound composed of six herbal medicines with significant antidepressant ***,the antidepressant mechanism of CG remains *** the present study,we attempted to elucidate the antidepressant mechanism of CG by regulating purine metabolism and purinergic ***,the regulatory effect of CG on purine metabolites in the prefrontal cortex(PFC)of chronic unpredictable mild stress(CUMS)rats was analyzed by ultra high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)targeted quantitative ***,purinergic receptors(P2X7 receptor(P2X7R),A1 receptor(A1R)and A2A receptor(A2AR))and signaling pathways(nod-like receptor protein 3(NLRP3)inflammasome pathway and cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)pathway)associated with purine metabolism were analyzed by western blotting and enzyme-linked immunosorbent assay(ELISA).Besides,antidepressant mechanism of CG by modulating purine metabolites to activate purinergic receptors and related signaling pathways was dissected by exogenous supplementation of purine metabolites and antagonism of purinergic receptors in *** in vivo study showed that the decrease in xanthine and the increase in four purine nucleosides were closely related to the antidepressant effects of ***,purinergic receptors(P2X7R,A1R and A2AR)and related signaling pathways(NLRP3 inflammasome pathway and cAMP-PKA pathway)were also significantly regulated by *** results of exogenous supplementation of purine metabolites and antagonism of purinergic receptors showed that excessive accumulation of xanthine led to activation of the P2X7R-NLRP3 inflammasome pathway,and the reduction of adenosine and inosine inhibited the A1R-cAMP-PKA pathway,which was significantly ameliorated by ***,CG could promote neuroprotection and ultimately play an antidepressant role by inhibiting the xanthine-P2X7R-NLRP3 inflammasome pathway and activating the adenosine/inosi

关键词： Chaigui granules Traditional Chinese medicine Antidepressant mechanism Purine metabolism Purinergic signaling

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Tumorigenic bacteria in colorectal cancer:mechanisms and treatments

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Cancer Biology & Medicine 2022年第2期19卷 147-162页

作者： Sha Li Jinyi Liu Xiangjin Zheng Liwen Ren Yihui Yang Wan Li Weiqi Fu Jinhua Wang guanhua du The State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing 100050China Key Laboratory of Drug Target Research and Drug Screen Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China

Colorectal cancer(CRC)is the third most common and the second most fatal *** recent years,more attention has been directed toward the role of gut microbiota in the initiation and development of *** bacterial species,such as Fusobacterium nucleatum,Escherichia coli,Bacteroides fragilis,Enterococcus faecalis,and Salmonella *** been associated with CRC,based upon sequencing studies in CRC patients and functional studies in cell culture and animal *** bacteria can cause host DNA damage by genotoxic substances,including colibactin secreted by pks+Escherichia coli,*** toxin(BFT)produced by Bacteroides fragilis,and typhoid toxin(TT)from *** bacteria can also indirectly promote CRC by influencing host-signaling pathways,such as E-cadherin/β-catenin,TLR4/MYD88/NF-κB,and SMO/RAS/p38 ***,some of these bacteria can contribute to CRC progression by helping tumor cells to evade the immune response by suppressing immune cell function,creating a proinflammatory environment,or influencing the autophagy *** with the classical antibacterial drugs,metronidazole or erythromycin,the antibacterial active ingredients,M13@Ag(electrostatically assembled from inorganic silver nanoparticles and the protein capsid of bacteriophage M13),berberine,and zerumbone,were found to inhibit tumorigenic bacteria to different *** this review,we described progress in elucidating the tumorigenic mechanisms of several CRC-associated bacteria,as well as progress in developing effective antibacterial *** bacteria have been shown to be active in the oncogenesis and progression of CRC,and some antibacterial compounds have shown therapeutic potential in bacteria-induced *** bacteria may be useful as biomarkers or therapeutic targets for CRC.

关键词： Colorectal cancer microbiota tumorigenic mechanism genotoxicity cancer pathways tumor immunity

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Theoretical and experimental cocrystal screening of temozolomide with a series of phenolic acids, promising cocrystal coformers

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Chinese Chemical Letters 2024年第5期35卷 407-417页

作者： Hongmei Yu Baoxi Zhang Meiju Liu Cheng Xing Guorong He Li Zhang Ningbo Gong Yang Lu guanhua du Beijing Key Laboratory of Polymorphic Drugs Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Beijing City Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

The virtual cocrystal screening approach based on molecular electrostatic potential surface(MEPS)maps is a fast and feasible computational method to estimate the probability of cocrystal formation by calculating the difference in the interaction site pairing energies of monomers and that of their assemblies prior to experimental *** this paper,we report 12 cocrystal forms of temozolomide with mono-,di-,and trihydroxy benzoic acids,namely,3-hydroxy-,2,4-dihydroxy-,2,5-dihydroxy-,2,6-dihydroxy-,3,4-dihydroxy-,and 3,4,5-trihydroxy-benzoic acids,as well as benzoic acid,as pharmaceutical coformers for the first time.10 single crystals out of the 12 cocrystal forms were obtained and unequivocally determined by single-crystal X-ray diffraction,which clarified spatial arrangements,molecular conformations,and supramolecular *** further gains some insights into the sites of hydrogen bonding interactions for exploring combination patterns in these *** stability of TMZ was successfully achieved by cocrystallization with these acids.

关键词： Virtual Cocrystal Temozolomide Supramolecular synthon Phenolic acid Stability

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Author correction to‘3-O-Acetyl-11-keto-bboswellic acid ameliorated aberrant metabolic landscape and inhibited autophagy in glioblastoma’[Acta Pharm Sin B 10(2020)301-312]

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3783-3784页

作者： Wan Li Liwen Ren Xiangjin Zheng Jinyi Liu Jinhua Wang Tengfei Ji guanhua du The State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Laboratory of Drug Target Research and Drug Screen Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China

The authors apologize for two inadvertent mistakes in Fig.5:the image of immunofluorescence of LC3B in the Vehicle group(Fig.5c)and the image of transmission electron microscope in the AKBA 10 mmol/L group(Fig.5f)were provided *** corrected Fig.5 is shown *** original data of these figures have been provided to the Editorial Office,and the corresponding authors or the Editorial Office can be contacted for original data access.

关键词： correction Office image

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Salvianolic acid A alleviates renal injury in systemic lupus erythematosus induced by pristane in BALB/c mice

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Acta Pharmaceutica Sinica B 2017年第2期7卷 159-166页

作者： Yihuang Lin Yu Yan Huifang Zhang Yucai Chen Yangyang He Shoubao Wang Lianhua Fang Yang Lv guanhua du State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular Diseases Beijing Key Laboratory of Drug Targets Identification and Drug Screening Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College Beijing Key Laboratory of Polymorphic Drugs Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

The purpose of this study was to investigate the effects of salvianolic acid A (SAA) in systemic lupus erythematosus (SLE) induced by pristane in BALB/c mice. Lupus mice were established by confirming elevated levels of autoantibodies and IL-6 after intraperitoneal injection of pristane. Mice were then treated with daily oral doses of SAA for 5 months in parallel with mice treated with prednisone and aspirin as positive controls. The levels of autoantibodies were monitored at monthly intervals and nephritic symptoms observed by hematoxylin and eosin(H&E) and periodic acid-Schiff (PAS) staining. Western blot analysis of renal tissue was also employed. SAA treatment caused a significant reduction in the levels of anti-Sm autoantibodies and reduced renal histopathological changes and pathological effects. SAA treatment also significantly inhibited the phosphorylation of IKK, I kappa B and NF kappa B in renal tissues of lupus mice. In conclusion, the results suggest that SAA alleviates renal injury in pristane-induced SLE in BALB/c mice through inhibition of phosphorylation of IKK, I kappa B and NF kappa B. (C) 2017 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Salvianolic acid A SLE Renal injury Autoantibodies Pristane BALB/c mice NF kappa B

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Pharmacokinetic study of gallocatechin-7-gallate from Pithecellobium clypearia Benth. in rats

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Acta Pharmaceutica Sinica B 2016年第1期6卷 64-70页

作者： Chao Li Xiaowei Song Junke Song Xiaocong Pang Zhe Wang Ying Zhao Wenwen Lian Ailin Liu guanhua du Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing Key Laboratory of Drug Target and Screening Research State Key Laboratory of Bioactive Substance and Function of Natural Medicines

The pharmacokinetic profile of gallocatechin-7-gallate (J10688) was studied in rats after intravenous administration. Male and female Sprague-Dawley (SD) rats received 1, 3, and 10 mg/kg (i.v.) of J10688 and plasma drug concentrations were determined by a high performance liquid chromatography-mass spectrometry (LC-MS) method. The pharmacokinetic software Data Analysis System (Version 3.0) was used to calculate the pharmacokinetic parameters. For different i.v. doses of J10688, the mean peak plasma concentration (C-0) values ranged from 11.26 to 50.82 mg/L, and mean area under the concentration -time curve (ALC(0-i)) values ranged from 1.75 to 11.80 (mg ' h/L). J10688 lacked dose dependent pharmacokinetic properties within doses between 1 and 10 mg/kg, based on the power model. The method developed in this study was sensitive, precise, and stable. The pharmacokinetic properties of J10688 in SD rats were shown to have rapid distribution and clearance values. These pharmacokinetic results may contribute to an improved understanding of the pharmacological actions of J10688. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Galloeatechin-7-gallata LC-MS Pharmacokinetics Dose proportionality Non-compartment mode

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The biology,function,and applications of exosomes in cancer

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Acta Pharmaceutica Sinica B 2021年第9期11卷 2783-2797页

作者： Jinyi Liu Liwen Ren Sha Li Wan Li Xiangjin Zheng Yihui Yang Weiqi Fu Jie Yi Jinhua Wang guanhua du The State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing 100050China Key Laboratory of Drug Target Research and Drug Screen Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Department of Clinical Laboratory Peking Union Medical College HospitalBeijing 100730China

Exosomes are cell-derived nano vesicles with diameters from 30 to 150 nm,released upon fusion of multi vesicular bodies with the cell *** can transport nucleic acids,proteins,and lipids for intercellular communication and activate signaling pathways in target *** cancers,exosomes may participate in growth and metastasis of tumors by regulating the immune response,blocking the epithelial-mesenchymal transition,and promoting *** are also involved in the development of resistance to chemotherapeutic *** in liquid biopsies can be used as non-invasive biomarkers for early detection and diagnosis of *** of their amphipathic structure,exosomes are natural drug delivery vehicles for cancer therapy.

关键词： Exosomes Tumor immunity Tumor metastasis Drug resistance Biomarkers Drug delivery

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Anti-inflammatory Effects and Mechanisms of Rhein, an Anthraquinone Compound, and Its Applications in Treating Arthritis: A Review

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Natural Products and Bioprospecting 2020年第6期10卷 445-452页

作者： Hongjuan Wang Dezhi Yang Li Li Shiying Yang guanhua du Yang Lu Beijing Key Laboratory of Polymorphic Drugs Center of Pharmaceutical PolymorphsInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Drug Targets Identification and Drug Screening National Center for Pharmaceutical ScreeningInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Inflammation is a defensive response of living tissues to damaging agents,which exists in two forms,acute inflammation and chronic inflammation,and chronic inflammation is closely related to ***,the commonly prescribed anti-inflammatory medications are greatly limited by high incidence of gastrointestinal erosions in the clinical ***,a bioactive constituent of anthraquinone,exhibits excellent anti-inflammatory activities and therapeutic effects on arthritis with less gastrointestinal *** there are numbers of studies on anti-inflammatory effects and mechanisms of rhein in the last few decades,to the best of our knowledge,only a few review articles pay attention to the interactive relationships of rhein on multiple inflammatory signaling pathways and cellular processes from a comprehensive ***,we summarized anti-inflammatory effects and mechanisms of rhein and its practical applications in the treatment of arthritis,thereby providing a reference for its basic researches and clinical applications.

关键词： Rhein Anthraquinone Anti-inflammatory Arthritis

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Antihyperuricemic effect of mangiferin aglycon derivative J99745 by inhibiting xanthine oxidase activity and urate transporter 1 expression in mice

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Acta Pharmaceutica Sinica B 2018年第2期8卷 306-315页

作者： Zhizhen Qin Shoubao Wang Yihuang Lin Ying Zhao Shengqian Yang Junke Song Tao Xie Jinlong Tian Song Wu guanhua du Beijing Key Laboratory of Drug Target and Screening Research Department of New Drug R&D Institute of Materia Medica Chinese Academy of Medical Science and Peking Union Medical College

A mangiferin aglycon derivative J99745 has been identified as a potent xanthine oxidase(XOD) inhibitor by previous in vitro study. This study aimed to evaluate the hypouricemic effects of J99745 in experimental hyperuricemia mice, and explore the underlying mechanisms. Mice were orally administered 600 mg/kg xanthine once daily for 7 days and intraperitoneally injected 250 mg/kg oxonic acid on the 7 th day to induce hyperuricemia. Meanwhile, J99745(3, 10, and 30 mg/kg), allopurinol(20 mg/kg) or benzbromarone(20 mg/kg) were orally administered to mice for 7 days. On the 7 th day,uric acid and creatinine in serum and urine, blood urea nitrogen(BUN), malondialdehyde(MDA) content and XOD activities in serum and liver were determined. Morphological changes in kidney were observed using hematoxylin and eosin(H&E) staining. Hepatic XOD, renal urate transporter 1(URAT1), glucose transporter type 9(GLUT9), organic anion transporter 1(OAT1) and ATP-binding cassette transporter G2(ABCG2) were detected by Western blot and real time polymerase chain reaction(PCR). The results showed that J99745 at doses of 10 and 30 mg/kg significantly reduced serum urate, and enhanced fractional excretion of uric acid(FEUA). H&E staining confirmed that J99745 provided greater nephroprotective effects than allopurinol and benzbromarone. Moreover, serum and hepatic XOD activities and renal URAT1 expression declined in J99745-treated hyperuricemia mice. In consistence with the ability to inhibit XOD, J99745 lowered serum MDA content in hyperuricemia mice. Our resultssuggest that J99745 exerts urate-lowering effect by inhibiting XOD activity and URAT1 expression, thus representing a promising candidate as an anti-hyperuricemia agent.

关键词： Antihyperuricemic effect Mangiferin aglycon Derivative Xanthine oxidase Urate transporter 1

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Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway

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Acta Pharmaceutica Sinica B 2021年第11期11卷 3465-3480页

作者： Xiangjin Zheng Wan Li Huanli Xu Jinyi Liu Liwen Ren Yihui Yang Sha Li Jinhua Wang Tengfei Ji guanhua du The State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Key Laboratory of Drug Target Research and Drug Screen Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Department of Pharmacology Capital Medical UniversityBeijing 100069China

Glioblastoma multiforme(GBM)in the central nervous system is the most lethal advanced glioma and currently there is no effective treatment for *** of sinomenine,an alkaloid from the Chinese medicinal plant,Sinomenium acutum,showed that it had inhibitory effects on several kinds of ***,we synthesized a sinomenine derivative,sino-wcj-33(SW33),tested it for antitumor activity on GBM and explored the underlying ***33 significantly inhibited proliferation and colony formation of GBM and reduced migration and invasion of U87 and U251 *** also arrested the cell cycle at G2/M phase and induced mitochondria-dependent *** gene enrichment analysis and pathway validation showed that SW33 exerted anti-GBM effects by regulating PI3 K/AKT and AMPK signaling pathways and significantly suppressed tumorigenicity with no obvious adverse effects on the ***33 also induced autophagy through the PI3 K/AKT/mTOR and AMPK/mTOR ***,SW33 appears to be a promising drug for treating GBM effectively and safely.

关键词： SW33 GBM G2/M phase Apoptosis Autophagy mTOR Anti-inflammation Safety

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