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1株具有抑制AchE活性的螃蟹内生真菌的鉴定及发酵条件优化

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中国食品学报 2013年第11期13卷 191-197页

作者：王凤舞青岛农业大学食品科学与工程学院山东青岛266109

目的:对螃蟹中分离的1株具有较高抑制AchE活性的内生真菌Pt-2进行菌种鉴定,并优化其发酵条件。方法:将Pt-2菌株进行形态学和分子生物学鉴定,并以发酵液AchE抑制率为活性指标,采用单因素和正交试验优化发酵时间、接种量、装液量、培养温... 详细信息

目的:对螃蟹中分离的1株具有较高抑制AchE活性的内生真菌Pt-2进行菌种鉴定,并优化其发酵条件。方法:将Pt-2菌株进行形态学和分子生物学鉴定,并以发酵液AchE抑制率为活性指标,采用单因素和正交试验优化发酵时间、接种量、装液量、培养温度和碳源、氮源等发酵条件。结果:经鉴定,Pt-2菌为枝孢菌属,命名为Cladosporium ***-2;其最佳发酵条件:发酵时间9 d、接种量5%、装液量200 mL(三角瓶容量500 mL)、培养温度25℃,以碳源为葡萄糖,氮源为酵母膏。在此条件下,发酵液提取物的乙酰胆碱酯酶抑制率较高,为76.32%。结论:螃蟹内生真菌具有较好的抑制AchE活性,为抗老年痴呆天然药物的开发提供了新的来源。

关键词：菌株鉴定枝孢菌属螃蟹内生真菌乙酰胆碱酯酶条件优化

Inhibitory Effect of Recombinant Endostatin on Angiogenesis and Tumor Growth of Hepatoma

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Journal of Huazhong university of science and technology(Medical sciences) 2003年第3期23卷 223-226页

作者：黎培员冯作化张桂梅张慧薛胜利黄波林菊生 institute of Liver Diseases Department of Biochemistry and molecular Biology

To study the influence of recombinant endostatin on angiogenesis and tumor growth of mice H22 hepatoma, tumor models were constructed by injecting H22 hepatoma cells into the leg muscle of mice Recombinant endostatin was produced by gene engineering in E coli The recombinant protein was injected subcutaneously to treat transplanted hepatoma faraway The weight of tumors was measured, and the changes of necrosis of tumor cells and vessel density were observed by immunohistochemistry The results suggested that the growth of hepatoma models transplanted in the muscle of legs was suppressed by recombinant endostatin The density of vacularity was decreased, but the necrosis of tumor cells increased The inhibitory effect of recombinant endostatin on angiogenesis and tumor growth of hepatoma was not affected after chemotherapy

关键词： endostatin tumor therapy anti-angiogenesis

Highly Efficient and Economical Baculovirus Expression System for Preparing Human Papillomavirus Type16 Virus-like Particle

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 548-552页

作者： Jin ZHENG, Jun MA, Xiao-feng YANG, Hong-Li LIU, Hong-Wei CHENG, Lu-Sheng SI, and Yi-Li WANGThe Key Laboratory of Biomedical Information engineering of Ministry of Education, institute of Cancer Research,School of Life science and technology, Xi’an Jiaotong university, Xi’an 710061, china The Key Laboratory of Biomedical Information engineering of Ministry of Education Institute of Cancer Research School of Life Science and Technology Xi’an Jiaotong University

To improve the existing human papillomavirus type16 (HPV16) virus-like particle (VLP) preparation, a highly efficient, economical and timesaving system was established. Sf-9 cells were infected with recombinant baculovirus containing the target gene encoding HPV16L1 protein with 6×His tag, and harvested 72 h postinfection (p.i.) at 27 °C. The ProBondTM purification system was used for protein purification. The molecular weight of expressed HPV16L1 protein was 58 kD as revealed by SDS-PAGE, and confirmed by Western blot. The purity of denatured and native HPVL1 proteins that were prepared were 91.9% and 71.5%, respectively, which corresponded to a yield of 2.26 mg denatured protein and 1.84 mg native protein per 2×107 cells. The proteins were further analyzed by mouse erythrocyte hemagglutination assay and hemagglutination inhibition assay, and there effects on VLP formation were also visualized by transmission electron microscopy. Results showed that the native protein purified was biologically active as natural HPVL1 protein, inducing the murine erythrocyte agglutination and VLP formation. In addition, the purified recombinant HPV16L1 native protein with 6×His tag could self-assemble into virions in vitro. Hopefully, the present expression and purification system is promising to be convenient, timesaving and economical for preparation of HPV16 VLP vaccine.

关键词： human papillomavirus HPV16L1 protein purification virus-like particle (VLP)

Axial enantiomers with donor-regulated TADF feature for multicolor circularly polarized electroluminescence

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science china chemistry 2024年第6期67卷 2039-2045页

作者： Chen-Hao Guo Wen-Long Zhao Ke-Ke Tan Wei-Chen Guo Liheng feng Chuan-feng Chen Meng Li School of chemistry and Chemical engineering Shanxi UniversityTaiyuan030006China Beijing National Laboratory for molecular sciences CAS Key Laboratory of Molecular Recognition and FunctionInstitute of ChemistryChinese Academy of SciencesBeijing100190China university of Chinese Academy of sciences Beijing100049China

This study optimized the TADF feature of axial chiral enantiomers through precise donor engineering,and multicolor CP-TADFwas achieved in the axial chiral *** pairs of axial enantiomers with donor-regulated TADF feature,namely(R/S)-TPCBD,(R/S)-DPCBD,and(R/S)-DPACBD,were synthesized by introducing carbazole donors with different substituents onto biphenyl cyanide *** the electron-donating ability of donors increases,the emission of these axial enantiomers ranges from 455,476 to 552 nm,their singlet-triplet energy gaps(ΔE_(ST))gradually decrease from 0.30,0.22 to 0.02 e V,accompanied by an increasement in the transition rate(k_(RISC))of RISC process,and the k_(RISC)of DPACBD could reach up to 7.16×10^(5)s^(-1).These axial enantiomers also exhibit mirror-image CD and circularly polarized luminescence(CPL)***,OLEDs based on TPCBD,DPCBD,and DPACBD as emitter were then fabricated,which displayed blue,green,and orange electroluminescence with EQE_(max)of 13.0%,16.4%,and 25.0%,*** results also exhibited a phenomenon of device efficiency increasing with the enhancement of donor ***,the CP-OLEDs using(R/S)-TPCBD,(R/S)-DPCBD and(R/S)-DPACBD as emitters displayed intense CPEL signals with g_(EL)values of 3.4×10^(-3)/-4.1×10^(-3),3.2×10^(-3)/-3.1×10^(-3)and2.3×10^(-3)/-2.1×10^(-3),*** convenient molecular engineering of donor regulation in the same molecular skeleton,CP-TADF materials with multicolor CPEL and improved device performance could be conveniently achieved.

关键词： axial chirality thermally activated delayed fluorescence circularly polarized luminescence electroluminescence organic light-emitting diodes

对氨基苯酚在玻碳电极上的电化学行为研究及测定

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当代化工 2003年第4期32卷 191-193页

作者：孙伟张琦韩军英青岛科技大学化学与分子工程学院山东青岛266042 山东教育学院山东济南250003

研究了对氨基苯酚 (PAP)在玻碳电极上的电化学行为。对氨基苯酚在玻碳电极上能够发生可逆的氧化还原反应 ,其单扫和多扫循环伏安图保持一致 ,在电极上的反应是扩散控制的过程。用示差脉冲伏安法对其测定条件进行了优化 ,在最佳测定pH 8.... 详细信息

研究了对氨基苯酚 (PAP)在玻碳电极上的电化学行为。对氨基苯酚在玻碳电极上能够发生可逆的氧化还原反应 ,其单扫和多扫循环伏安图保持一致 ,在电极上的反应是扩散控制的过程。用示差脉冲伏安法对其测定条件进行了优化 ,在最佳测定pH 8.4的Tris缓冲溶液中 ,PAP的浓度与峰电流在 1.0× 10 -7～ 1.0× 10 -4mol/L范围内呈线性关系。

关键词：对氨基苯酚玻碳电极伏安法

Prescreening of large-effect markers with multiple strategies improves the accuracy of genomic prediction

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Journal of Integrative Agriculture 2024年第5期23卷 1634-1643页

作者： Keanning Li Bingxing An Mang Liang Tianpeng Chang Tianyu Deng Lili du Sheng Cao Yueying du Hongyan Li Lingyang Xu Lupei Zhang Xue Gao Junya LI Huijiang Gao Laboratory of molecular Biology and Bovine Breeding Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Shaanxi Key Laboratory of molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China College of Computer and Information engineering Tianjin Agricultural UniversityTianjin 300384China College of Animal science and technology Qingdao Agricultural UniversityQingdao 266109China Tongliao Animal Agriculture Development Service Center Tongliao 028000China

Presently,integrating multi-omics information into a prediction model has become a ameliorate strategy for genomic selection to improve genomic prediction ***,we set the genomic and transcriptomic data as the training population data,using BSLMM,TWAS,and eQTL mapping to prescreen features according to |β_(b)|>0,top 1%of phenotypic variation explained(PVE),expression-associated single nucleotide polymorphisms(eSNPs),and egenes(false discovery rate(FDR)1),where these loci were set as extra fixed effects(named GBLUP-Fix)and random effects(GFBLUP)to improve the prediction accuracy in the validation population,*** results suggested that both GBLUP-Fix and GFBLUP models could improve the accuracy of longissimus dorsi muscle(LDM),water holding capacity(WHC),shear force(SF),and pH in Huaxi cattle on average from 2.14 to 8.69%,especially the improvement of GFBLUP-TWAS over GBLUP was 13.66%for *** methods also captured more genetic variance than *** study confirmed that multi-omics-assisted large-effects loci prescreening could improve the accuracyofgenomic prediction.

关键词： multi-omics data features prescreening eQTL mapping Huaxi cattle genomic selection

A robust viscoelastic surfactant tolerating 20% HCl up to 150℃ for oil well stimulation

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Petroleum science 2024年第3期21卷 1865-1874页

作者： Ji Wang Ning Qi Hong-Yao Yin Yu-Jun feng Polymer Research institute State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu610065SichuanPR.China Tianfu Yongxing Laboratory New Theory and Technology of CO_(2) Capture Research CenterChengdu610065SichuanPR China School of Petroleum engineering China University of Petroleum(East China)Qingdao266580ShandongPR China

Viscoelastic surfactants(VES)are often used as viscous diverters in acidizing stimulation to prolong the acid consumption time and maximize zonal coverage of the acid for improving well ***,the ceiling temperature of commercial VES cannot exceed 120℃in practical use because of the poor thermal stability and fragile molecular structure,hindering their implementation in hightemperature oil reservoirs,i.e.,≥150℃.Here we synthesized a novel C22-tailed diamine,N-erucaminopropyl-N,N-dimethylamine(EDPA),and examined comparatively its rheological behavior,assemblies morphology and molecular stability in 20 wt%HCl with a commercial VES,erucyl dimethyl amidopropyl betaine(EDAB).The feasibility of EDPA for acidizing stimulation was assessed by acid etching of carbonate rock with its HCl solution at 150℃.Rheological results showed that the 2.5 wt%EDPA—20 wt%HCl solution maintains stable viscosity of 90 m Pa s at 150℃for 60 min,while that of 2.0 wt%EDAB HCl solution is just 1 m Pa s under identical conditions.1H NMR spectra and cryo-TEM observations revealed that the chemical structure and self-assembled architectures of EDPA remained intact in such context,but the EDAB suffered from degradation due to the hydrolysis of the amide group,accounting for the poor heat-resistance and *** reaction rate of 2.5 wt%EDPA HCl solution with carbonate rock was one order of magnitude lower than that of 20 wt%HCl solution at 150℃,underpinning the potential of EDPA to be used in the high-temperature reservoirs *** work improved the thermal tolerance of VES in highly concentrated HCl solution,paving a feasible way for the acidization of high-temperature reservoir environments(~150℃).

关键词： Viscoelastic surfactants Thermal stability Self-diverting acid Wormlike micelles Acidizing stimulation

新型查尔酮衍生物抗乳腺癌活性的3D-QSAR模型构建及分子设计

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南京理工大学学报 2024年第2期48卷 248-252页

作者：陈艳冯惠冯长君堵锡华徐州工程学院材料与化学工程学院江苏徐州221018

为了获得较高抗乳腺癌活性的新型化合物,对18个新型查尔酮衍生物抗乳腺癌活性(pIC_(50))进行了三维定量构效关系(3D-QSAR)研究。其中14个化合物作为训练集用于构建3D-QSAR模型,其余化合物(含模板分子)作为测试集对所建模型进行验证。所... 详细信息

为了获得较高抗乳腺癌活性的新型化合物,对18个新型查尔酮衍生物抗乳腺癌活性(pIC_(50))进行了三维定量构效关系(3D-QSAR)研究。其中14个化合物作为训练集用于构建3D-QSAR模型,其余化合物(含模板分子)作为测试集对所建模型进行验证。所建3D-QSAR模型的交叉验证系数R^(2)_(CV)为0.569,非交叉验证系数R^(2)为0.974,说明所建模型具有良好的稳定性和预测能力。该模型中立体场、静电场对pIC_(50)的贡献分别为58.8%和41.2%,表明影响该类化合物抗肿瘤活性的主要因素是取代基的疏水性、空间位阻和电荷分布。通过对模型的分析,设计了5个具有较高pIC_(50)的新化合物,有待通过后续医学实验加以验证。

关键词：新型查尔酮衍生物抗乳腺癌活性三维定量构效关系分子设计

Enhancing the peroxidase-like activity of MoS_(2)-based nanozymes by introducing attapulgite for antibacterial application and sensitive detection of glutathione

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Nano Research 2024年第8期17卷 7415-7426页

作者： feng feng Yihe Zhang Xiao Zhang Bin Mu Jiahe Zhang Wenjie Qu Wangshu Tong Minmin Liang Qi An Zhanjun Guo Lu Zhao engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid WastesNational Laboratory of Mineral MaterialsSchool of Materials Science and TechnologyChina University of Geosciences(Beijing)Beijing 100083China Key Laboratory of Clay Mineral Applied Research of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Experimental Center of Advanced Materials School of Materials science and engineering School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China

Nanozymes are next-generation of nanomaterials with enzyme-like *** particular,nanozymes with peroxidase(POD)-like activity have been utilized in various fields,including antibacterial,detection,degradation,***,their extensive applications were limited by their low catalytic activity ***,we have presented a composite nanozyme based on attapulgite(ATP)(Fe-ATP-MoS_(2)(FAM)),which exhibited enhanced POD-like activity(185.33 U·mg^(−1)),4.25 times higher than that of Fe-MoS_(2)(FM)(43.63 U·mg^(−1)).The density functional theory(DFT)calculations indicated that the addition of ATP increased the electron density of metal centers(Mo and Fe).More importantly,Michaelis–Menten kinetics revealed that the introduction of ATP significantly enhanced the binding affinities of substrates through the pores of ATP,forming a highly concentrated substrate microenvironment and thus promoting its POD-like ***,from molecular size and kinetic analysis,we proposed that the changes in substrate size before and after oxidation also significantly affected its Michaelis-constant(Km)***,we utilized FAM in the applications of highly effective antibacterial application and sensitive detection of glutathione(GSH).In conclusion,this work provides a novel approach for designing a highly efficient nanozyme based on natural mineral composites.

关键词： attapulgite peroxidase electron density affinity antibacterial