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Synergistic effects of multiple rotors and hydrogen-bond interactions lead to sensitive near-infrared viscosity probes for live-cell microscopy

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Science China Chemistry 2023年第8期66卷 2329-2338页

作者： dongyang li Tianruo Shen Xiaoqi Xue Weijie Chen Wenjun Tao Weijie Chi Sheng Hua liu Ying Tan Xiaogang liu Jun Yin National Key Laboratory of Green Pesticide International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan430079China Science Mathematics and Technology ClusterSingapore University of Technology and DesignSingapore487372Singapore State Key Laboratory of Chemical Oncogenomics Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen518055China Department of Chemistry School of ScienceHainan UniversityHaikou570228China

Changes in cellular viscosity are associated with various physiological processes and pathological *** study these cellular processes and functions,highly sensitive fluorescent probes that detect subtle changes in viscosity are urgently needed but remain *** this study,we present a series of viscosity-responsive near-infrared(NIR)fluorescent probes based on styrene-coated boron dipyrromethene(BODIPY).The probe modified with dimethylaminostyrene and piperazine at the two terminals of the BODIPY scaffold showed extremely high viscosity sensitivity values(x,around 1.54),with excellent performance for detecting viscosity below 20 c *** outstanding property is attributed to the synergistic effects of multiple rotatable bonds and hydrogen-bond ***,this probe has been successfully deployed to monitor viscosity changes in various cellular compartments(i.e.,cytoplasm)and processes(such as during autophagy).This work provides a rational molecular design strategy to construct fluorescent probes with high viscosity sensitivity for exploring cell functions.

关键词： fluorescent probe high viscosity sensitivity multiple rotors hydrogen bonds bioimaging

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Effects of soil metal(loid)s pollution on microbial activities and environmental risks in an abandoned chemical smelting site

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Journal of Environmental Sciences 2024年第9期143卷 60-70页

作者： Hao li Jun Yao Jianli liu Ning Min Geoffrey Sunahara dongyang Men Robert Duran School of Water Resources and Environment Research Center of Environmental Science and EngineeringChina University of Geosciences(Beijing)Beijing 100083China Department of Natural Resource Sciences McGill University21111Lakeshore DriveSte-Anne-de-BellevueQuebecH9X 3V9Canada Equipe Environnement et Microbiologie MELODY groupUniversitéde Pau et des Pays de l’AdourE2S-UPPAIPREM UMR CNRS 5254BP 115564013 Pau CedexFrance

Abandoned chemical smelting sites containing toxic substances can seriously threaten and pose a risk to the surrounding ecological *** samples were collected from different depths(0 to 13 m)and analyzed for metal(loid)s content and fractionation,as well as microbial *** potential ecological risk indices for the different soil depths(ordered from high to low)were:1 m(D-1)>surface(S-0)>5 m(D-5)>13 m(D-13)>9 m(D-9),ranging between 1840.65-13,089.62,and representing extremely high environmental risks,of which Cd(and probably not arsenic)contributed to the highest environmental risk.A modified combined pollution risk index(MCR)combining total content and mobile proportion of metal(loid)s,and relative toxicities,was used to evaluate the degree of contamination and potential environmental *** the near-surface samples(S-0 and D-1 layers),the MCR considered that As,Cd,Pb,Sb,and Zn achieved high and alarming degrees of contamination,whereas Fe,Mn,and Ti were negligible or low to moderate pollution *** microcalorimetry and enzymatic activity measurements of contaminated soil samples were used to assess the microbial metabolic activity *** analysis elucidated the relationship between metal(loid)s exchangeable fraction or content and microbial activity characteristics(plic activity in the D-1 layer was low presumably due to heavy metal *** activity indicators and microcalorimetric growth rate(k)measurements were considered sensitive indicators to reflect the soil microbial activities in abandoned chemical smelting sites.

关键词： Abandoned chemical smelting sites Microbial activities Modified combined pollution risk Microcalorimetry Enzymatic activities

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青砧1号自根砧与不同苹果品种嫁接的苗木生长表现

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落叶果树 2024年第2期56卷 70-73页

作者：陈培琴张国庆仇智虎朱斌李东阳宁夏林业技术推广总站宁夏银川750000 宁夏林权服务与产业发展中心宁夏银川750000 宁夏林草局场站管理处宁夏银川750000

对引进的青砧1号自根砧容器苗进行培育1年,春季以青砧1号做基砧,分别与米奇拉、瑞阳、瑞雪、秦脆、威海金、烟富6、烟富10、烟优、水晶秦冠、美味、众成1号、鲁丽、瑞香红等13个苹果品种进行嫁接,对培育的青砧1号矮化自根砧苹果苗木的... 详细信息

对引进的青砧1号自根砧容器苗进行培育1年,春季以青砧1号做基砧,分别与米奇拉、瑞阳、瑞雪、秦脆、威海金、烟富6、烟富10、烟优、水晶秦冠、美味、众成1号、鲁丽、瑞香红等13个苹果品种进行嫁接,对培育的青砧1号矮化自根砧苹果苗木的根系、地上部进行观测研究。结果表明:青砧1号自根苗在宁夏地区栽培成活率高,嫁接的13个苹果品种青砧1号矮化自根砧苹果苗成活率均较高,亲和性好,苗木根系发达,分枝能力强,生长健壮。嫁接培育1年苗出圃质量分级均达到3级以上标准。

关键词：青砧1号自根苗苹果品种砧穗组合生长表现

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Impact assessment of genetically modified herbicide-tolerant cotton on arthropod communities

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Journal of Cotton Research 2022年第2期5卷 164-172页

作者： li dongyang ZHU Xiangzhen ZHANG Kaixin WANG li ZHANG Rui liANG Chengzhen LUO Junyu CUI Jinjie State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000 China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China.

Background:Cotton(Gossypium spp.)is one of the most important economic crops worldwide,and its production plays an important role in the economy of many *** modified herbicide-tolerant(GMHT)crops,which were developed to minimize the losses caused by weeds,have gradually become the most widely adopted genetically modified crops in the world due to their economic and environmental ***,the potential ecological and environmental risks of GMHT crops have attracted extensive attention and *** communities form a promine nt part of the biodiversity of agroecosystems and are important indicators of environmental *** the effects of GMHT crops on the diversity of arthropod communities is necessary to ensure the safety of GMHT ***:In this 2-year study,we investigated the potential impact of GMHT crops on arthropod *** GMHT cotton variety GGK2 with glyphosate tolerance and its near-isogenic non-GMHT variety K312 were used for the experimental *** Shannon diversity index(H),Simpson diversity index(D),Pielou evenness index(J),and principal co-ordinates analysis(PCoA)of the Bray-Curtis distance were used to evaluate the population dynamics and biodiversity of arthropods in cotton *** significant differences were found between GGK2 and K312 in their total abundance of arthropod communities,and biodiversity indexes on most sampling *** arthropod composition in the GGK2 and K312 plots was *** elates had a significant effect on biodiversity indexes,whereas no clear tendencies related to cotton variety or cotton variety x sampling elates interaction were *** addition,PCoA revealed high similarity between the arthropod communities in the plots of the GMHT cotton variety GGK2 and its near-isogenic variety ***:There was no obvious difference in abundance,diversity indexes of arthropod communities between GMHT cotto n variety GGK2 and its near-isogenic var

关键词： GMHT crops Cotton Arthropod communities Biodiversity Diversity index PCoA

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Supporting IrO_(x) nanosheets on hollow TiO_(2) for highly efficient acidic water splitting

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Nano Research 2024年第8期17卷 6903-6909页

作者： Ge Yu Ruilong li Yanmin Hu Xingen lin Ze lin dongyang Wu Gongming Wang Xun Hong Center of Advanced Nanocatalysis(CAN) Department of Applied ChemistryUniversity of Science and Technology of ChinaHefei 230026China

The efficiency of proton exchange membrane water electrolysis(PEM-WE)for hydrogen production is heavily dependent on the noble metal iridium-based ***,the scarcity of iridium limits the large-scale application of *** address this issue,it is promising to select an appropriate support because it not only enhances the utilization efficiency of noble metals but also improves mass transport under high ***,we supported amorphous IrO_(x) nanosheets onto the hollow TiO_(2) sphere(denoted as IrO_(x)),which demonstrated excellent performance in acidic electrolytic water ***,the annealed IrO_(x)catalyst at 150℃in air exhibited a mass activity of 1347.5 A·gIr^(−1),which is much higher than that of commercial IrO_(2) of 12.33 A·gIr^(−1) at the overpotential of 300 mV for oxygen evolution reaction(OER).Meanwhile,the annealed IrO_(x) exhibited good stability for 600 h operating at 10 mA·cm^(−2).Moreover,when using IrO_(x) and annealed IrO_(x) catalysts for water splitting,a cell voltage as low as 1.485 V can be achieved at 10 mA·cm^(−2).The cell can continuously operate for 200 h with negligible degradation of performance.

关键词： oxygen evolution reaction hydrogen evolution reaction amorphous IrO_(x) hollow TiO_(2) proton exchange membrane water electrolysis

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Electromagnetic anisotropic homogeneous model for eddy-current field in single-phase wound core

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High Voltage 2024年第1期9卷 172-181页

作者： lijun Zhou Woyang li Yingyi Xia Jiawei Chen Chenqingyu Zhang Xiaohu Cai Lei Guo dongyang Wang School of Electrical Engineering Southwest Jiaotong UniversityChengduChina

The analysis of the eddy-current field in the wound core widely used in the field of transformer energy conservation is taken as the *** the homogenisation idea to consider the unique geometry of the wound core and features of its equivalent multi-stage circular cross-section magnetic boundary,a homogeneous model consisting of a columnar material with continuous homogeneous electromagnetic anisotropy is established by deriving the Maxwell equations of the magnetic quasi-static field in the columnar coordinate ***,a homogeneous fine element model for the eddycurrent field in the wound core is established and the accuracy of the model has been verified by the test *** result shows that the homogeneous model can be effectively used for the analysis of the eddy-current field in the wound core,and the error of calculating the eddy-current loss under different excitation conditions is less than 6%under the premise of extremely saving the engineering calculation cost,which will help improve the operational performance of the wound core and contribute to the energysaving goal of the high-voltage equipment.

关键词： anisotropic coordinate excitation

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Asymmetric frequency multiplexing topological devices based on a floating edge band

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Photonics Research 2024年第6期12卷 1201-1212页

作者： JIAJUN MA CHUNMEI OUYANG YUTING YANG dongyang WANG HONGYI li li NIU YI liU QUAN XU YANFENG li ZHEN TIAN JIAGUANG HAN WEIli ZHANG Center for Terahertz Waves and College of Precision Instrument and Optoelectronics Engineering and Key Laboratory of Optoelectronics Information and TechnologyTianjin UniversityTianjin 300072China School of Materials Science and Physics China University of Mining and TechnologyXuzhou 221116China Optoelectronics Research Centre University of SouthamptonSouthampton SO171BJUK Guangxi Key Laboratory of Optoelectronic Information Processing School of Optoelectronic EngineeringGuilin University of Electronic TechnologyGuilin 541004China School of Electrical and Computer Engineering Oklahoma State UniversityStillwaterOklahoma 74078USA

Topological photonics provides a platform for robust energy transport regardless of sharp corners and ***,the frequency multiplexing topological devices have attracted much attention due to the ability to separate optical signals by wavelength and hence the potential application in optical communication *** frequency multiplexing topological devices are generally based on the slow light ***,the resulting static local spatial mode or finely tuned flat band has zero-group velocity,making it difficult for both experimental excitation and channel ***,we propose and experimentally demonstrate an alternative prototype of asymmetric frequency multiplexing devices including a topological rainbow and frequency router based on floating topological edge mode(instead of localized ones);hence the multiple wavelength channels can be collectively excited with a point source and efficiently routed to separate output *** channel separation in our design is achieved by gradually tuning the band gap truncation on a topological edge band over a wide range of frequencies.A crucial feature lies in that the topological edge band is detached from bulk states and floating within the upper and lower photonic band *** interestingly,due to the sandwiched morphology of the edge band,the top and bottom band gaps will each truncate into transport channels that support topological propagation towards opposite directions,and the asymmetrical transportation is realized for the frequency multiplexing topological devices.

关键词： tuning floating hence

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Dynamics and diversity of symbiotic bacteria in Apolygus lucorum at different developmental stages

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Journal of Cotton Research 2023年第1期6卷 37-47页

作者： XUE Hui ZHU Xiangzhen WANG li ZHANG Kaixin li dongyang JI Jichao NIU lin GAO Xueke LUO Junyu CUI Jinjie State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Zhengzhou Research Base State Key Laboratory of Cotton Biology/Zhengzhou UniversityZhengzhou 450001HenanChina Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory/College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina

Background Apolygus lucorum is a worldwide omnivorous pest damaging a range of crops and causing great economic *** bacteria living in insects play a key role in the nutrition,physiology,and behavior of ***,we present an experiment using Illumina HiSeq sequencing targeting the V3–V4 regions of bacteria’s 16S rRNA throughout the entire life cycle of *** The first and second instar nymphs have the largest alpha diversity compared with other life stages of the *** phyla Proteobacteria(72.29%),Firmicutes(15.24%),Actinobacteria(7.76%)exhibit the largest relative abundance in all developmental ***(23.97%)and Lactococcus(10.62%)are the two genera with the high-est relative *** relative abundance of Erwinia in the nymph stage is significantly greater than the adult stage,and the relative abundance of Lactococcus in 6-day-old and 9-day-old adult females is higher compared with adult *** These results reveal that microbial community composition and relative abundance shift dynamically at different life stages,implying that different bacterial phyla and genera may have specific roles in specific life stages such as metabolism,nutrition absorption,detoxification,and *** study reveals for the first time the community composition and ecological dynamics of symbiotic bacteria throughout the life stages of ***,and thus may provide insight to new strategies for pest control.

关键词： Symbiotic bacteria Apolygus lucorum life cycle Pest control Community composition Relative abundance Community richness

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Comparative transcriptional analysis and identification of hub genes associated with wing differentiation of male in Aphis gossypii

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Journal of Cotton Research 2022年第3期5卷 243-260页

作者： HUANGFU Ningbo SHI Qingyu CHEN Lulu MA Xiaoyan ZHANG Kaixin li dongyang WANG li ZHU Xiangzhen JI Jichao LUO Junyu CUI Jinjie Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China College of Agronomy Xinjiang Agricultural UniversityUrumqi 830052China.

Background:Aphis gossypii Glover(Hemiptera:Aphididae),a worldwide polyphagous phloem-feeding agricultural pest,has three wing morphs(winged parthenogenetic female,gynopara,and male)in the life *** exclusive males could fly from summer hosts to winter hosts,which are essential for gene exchanges of cotton aphid populations from different hosts or ***,the molecular mechanism of wing differentiation of male in *** remains ***:Morphological observation of male *** showed that there is no distinct difference in the external morphologies of the 1st and 2nd instar *** obvious differentiation of wing buds started in the 3rd instar nymph and was visible via naked eyes in the 4th instar nymphal stage,then adult male emerged with full *** to morphological dynamic changes,the development of wings in males were divided into four stages:preliminary stage(the 1st instar to 2nd instar),prophase(the 3rd instar),metaphase(the 4th instar),anaphase(the 5th instar).Results of feeding behavior monitoring via EPG(electrical penetration graph)technology indicated that although the male cotton aphids had strong desire to feed(longer duration of C 55.24%,F 5.05%and Pd waves 2.56%),its feeding efficiency to summer host cotton was low(shorter E13.56%and E2 waves 2.63%).Dynamic transcriptome analysis of male aphid at 5 different developmental periods showed that in the 3rd instar nymph,the number of up-regulated DEGs was significant increased,and time-course gene transcriptional pattern analyses results also showed that numerous genes categorized in clusters 3,5,and 8 had the highest expressed levels,which were consistent with morphological changes of wing *** results indicate that the 3rd instar nymph is the critical stage of wing bud differentiation in ***,through pathway enrichment analysis of DEGs and WGCNA,it revealed that the neuroactive ligand-receptor interaction,Ras signaling pathway,dopaminergic synaps

关键词： Aphis gossypii EPG Wing bud differentiation Dynamic transcriptome WGCNA

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药物减轻心肌缺血再灌注损伤机制研究的进展

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兰州大学学报（医学版） 2024年第4期50卷 88-94页

作者：邢阳冷玉芳赵潇李东阳兰金辉刘振李东斌王玉常利娅赵紫岑兰州大学第一临床医学院甘肃兰州730000 兰州大学第一医院麻醉手术科甘肃兰州730000 西安交通大学第二附属医院消化内科陕西西安710004 吉林大学白求恩第三临床医学院吉林长春130000

心肌缺血再灌注损伤常见于心肌梗塞、心肌缺血等,其发病率、死亡率高,且缺乏防治措施。积极探究防治心肌缺血再灌注损伤是临床研究的热点,近5年有关药物减轻心肌缺血再灌注损伤及其机制的研究较多,也取得了一些进展。本文着重介绍近5年... 详细信息

心肌缺血再灌注损伤常见于心肌梗塞、心肌缺血等,其发病率、死亡率高,且缺乏防治措施。积极探究防治心肌缺血再灌注损伤是临床研究的热点,近5年有关药物减轻心肌缺血再灌注损伤及其机制的研究较多,也取得了一些进展。本文着重介绍近5年关于药物通过抑制铁死亡、细胞凋亡、坏死性凋亡、细胞焦亡、氧化应激、钙超载、自噬调控、内质网应激、能量代谢等机制减轻心肌缺血再灌注损伤的实验研究和临床观察,及利用纳米技术、冠状动脉内给药技术等防治心肌缺血再灌注损伤的新成果,为心肌缺血再灌注损伤防治和研究提供理论依据。

关键词：药物心肌缺血再灌注器官保护细胞凋亡自噬氧化应激

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