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Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes

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Insect Science 2021年第5期28卷 1382-1398页

作者： Jian Hu Yan Du meng meng Yipei dong Jiewen Peng School of Agriculture Sun Yat-Sen UniversityGuangzhou510275China State key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhou510275China

Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune *** on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were *** and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and *** cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign *** results also suggested that granulocytes and plasmatocytes may express some different *** comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR *** genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.

关键词： cell sublines granulocyte hemocyte insect molecular marker plasmatocyte

Magnetron sputtered nickel oxide with suppressed interfacial defect states for efficient inverted perovskite solar cells

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Journal of Energy Chemistry 2025年第1期100卷 348-355页

作者： Guoqiang Ma Qin Tan Zhaoning Li Jingwei Xiu Jiafeng Wang Tianle Cheng dong He Qiang Sun Xuhang Ma Francesco Lamberti Zhubing He Department of Materials Science and Engineering Institute of Innovative Materials (I2M) Shenzhen Key Laboratory of Full Spectral Solar Electricity Generation (FSSEG)Southern University of Science and Technology (SUSTech) Core Research Facilities Southern University of Science and Technology Department of Chemical Sciences Department of Industrial Engineering University of Padova

Widely used spin-coated nickle oxide （NiOx） based perovskite solar cells often suffer from severe interfacial reactions between the NiOxand adjacent perovskite layers due to surface defect states,which inherently impair device performance in a long-term view,even with surface molecule *** this study,we developed high-quality magnetron-sputtered NiOxthin films through detailed process optimization,and compared systematically sputtered and spin-coated NiOxthin film surfaces from materials to *** sputtered NiOxfilms exhibit improved crystallinity,smoother surfaces,and significantly reduced Ni3+or Ni vacancies compared to their spin-coated ***,the interface between the perovskite and sputtered NiOxfilm shows a substantially reduced density of defect *** solar cells （PSCs） fabricated with our optimally sputtered NiOxfilms achieved a high power conversion efficiency （PCE） of up to 19.93%and demonstrated enhanced stability,maintaining 86.2% efficiency during 500 h of maximum power point tracking under one standard sun ***,with the surface modification using (4-(2,7-dibromo-9,9-dimethylacridin-10（9H）-yl)butyl)p hosphonic acid （DMAcPA）,the device PCE was further promoted to 23.07%,which is the highest value reported for sputtered NiOxbased PSCs so far.

关键词： Nickle oxide Magnetron sputter Defect Stability Perovskite solar cell

Adaptive vertical handoff algorithm in heterogeneous networks

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The Journal of China Universities of Posts and Telecommunications 2007年第1期14卷 22-25,44页

作者： XIE Sheng-dong WU meng Communication and Information Engineering Institute Nanjing University of Posts and Telecommunications Nanjing 210003 China

The integration of cellular network （CN） and wireless local area network （WLAN） is the trend of the next generation mobile communication systems, and nodes will handoff between the two kinds of networks. The received signal strength （RSS） is the dominant factor considered when handoff occurs. In order to improve the handoff efficiency, this study proposes an adaptive decision algorithm for vertical handoff on the basis of fast Fourier transform （FFT）. The algorithm makes handoff decision after analyzing the signal strength fluctuation which is caused by slow fading through FFT. Simulations show that the algorithm reduces the number of handoff by 35%, shortens the areas influenced by slow fading, and enables the nodes to make full use of WLAN in communication compared with traditional algorithms.

关键词： vertical handoff signal strength slow fading fast Fourier transform

Phase-field modeling of epitaxial growth with the Ehrlich-Schwoebel barrier: Model validation and application

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Science China(Technological Sciences) 2015年第4期58卷 753-762页

作者： dong Xiang Lei XING Hui sha sha CHEN Chang Le NIU Li Wei WANG Jian Yuan JIN Ke Xin shanxi Key Laboratory for Condensed Matter Structure and Properties Department of Applied Physics Northwestern Polytechnical University

In this paper,we introduce different forms of mobility into a quantitative phase-field model to produce arbitrary Ehrlich-Schwoebel(ES)*** studies were carried out in the one-side step-flow model,which showed that the original mobility not only induces the ES effect,but also leads to larger numerical instability with increase of the step ***,another modified form of the ES barrier is proposed,and is found to be more suitable for large-scale *** applications were performed on the wedding-cake structure,coarsening and coalescence of islands and spiral *** results show that the ES barrier exhibits more significant kinetic effects at the larger deposition rates by limiting motions of atoms on upper steps,leading to aggregation on the top layers,as well as the roughening of growing surfaces.

关键词： epitaxial growth phase-field method Ehrlich-Schwoebel barrier kinetics interfacial

Targeted energy transfer of a parallel nonlinear energy sink

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Applied Mathematics and Mechanics(English Edition) 2019年第5期40卷 621-630页

作者： Yimin WEI sha WEI Qianlong ZHANG Xinjian dong Zhike PENG Wenming ZHANG State Key Laboratory of Mechanical System and Vibration Shanghai Jiao Tong University

A parallel nonlinear energy sink(NES) is proposed and analyzed. The parallel NES is composed of a vibro-impact(VI) NES and a cubic NES. The dynamical equation is given, and the essential analytical investigation is carried out to deal with the cubic nonlinearity and impact nonlinearity. Multiple time-scale expansion is introduced, and the zeroth order is derived to give a rough outline of the system. The underlying Hamilton dynamic equation is given, and then the optimal stiffness is expressed. The clearance is regarded as a critical factor for the VI. Based on the periodical impact treatment by analytical investigation, the relationships of the cubic stiffness, the clearance, and the zeroth-order attenuation amplitude of the linear primary oscillator(LPO) are obtained.A cubic NES under the optimal condition is compared with the parallel NES. Harmonic signals, harmonic signals with noises, and the excitation generated by a second-order?lter are considered as the potential excitation forces on the system. The targeted energy transfer(TET) in the designed parallel NES is shown to be more e?cient.

关键词： targeted energy transfer (TET) nonlinear energy sink (NES) nonlinear vibration absorber impact vibration reduction

Application of Molecular Markers Linking to Cytoplasmic Male Sterile Loci to Assist Maintainer Line Selection and Their Selection Efficiency in Welsh Onion (Allium fistulosum L.)

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Agricultural Sciences in China 2010年第11期9卷 1571-1576页

作者： GAI Shu-peng meng Xiang-dong College of Life Science Qingdao Agricultural University Qingdao 266109 P.R.China College of Horticulture Shandong Agricultural University Tai ＇an 271018 P.R. China

Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the breeding of cytoplasmic male sterility in welsh onion has a little progress due to the limitation of its biological characteristic and traditional selection approach. To study the feasibility and the efficiency of utilizing marker assisted selection for male sterile lines in welsh onion, one SCAR marker, SCS13, and one RAPD marker, S2002400, which could distinguish between N and S cytoplasm in several welsh onion cultivars, were identified. The two markers were then confirmed by Southern blotting, and used to screen the N or S cytoplasm of individual plants in seven welsh onion cultivars in this study. Male sterile and fertile plants were evaluated by aceto-carmine dying. The frequency of N-cytoplasmic plants and maintainer genotype was calculated in the seven open populations of welsh onion. The minimum number of plants needed to identify a maintainer was evaluated to be 95% reliable. Results showed that 20 to 80% decrease of crosses and self-crosses for identifying a maintainer genotype could be achieved by the marker-assisted selection compared with traditional selection method. It was proved that the molecular markers could precisely identify cytoplasmic types individually, performed by one generation of cross and two generations of testcrosses and self-crosses. Finally, several maintainer genotype plants were selected with the help of the two markers in the seven cultivars. The screened markers could assist and accelerate sterile and maintainer lines selection with less labor and cost.

关键词： Alliumfistulosum L. CMS (cytoplasmic male sterility) markers-assisted selection molecular marker efficiency

Uncertainty quantification for stochastic dynamical systems using time-dependent stochastic bases

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Applied Mathematics and Mechanics(English Edition) 2019年第1期40卷 63-84页

作者： Jinchun LAN Qianlong ZHANG sha WEI Zhike PENG Xinjian dong Wenming ZHANG State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong University

A novel method based on time-dependent stochastic orthogonal bases for stochastic response surface approximation is proposed to overcome the problem of significant errors in the utilization of the generalized polynomial chaos(GPC) method that approximates the stochastic response by orthogonal polynomials. The accuracy and effectiveness of the method are illustrated by different numerical examples including both linear and nonlinear problems. The results indicate that the proposed method modifies the stochastic bases adaptively, and has a better approximation for the probability density function in contrast to the GPC method.

关键词： uncertainty quantification stochastic response surface approximation timedependent orthogonal bases polynomial chaos

Estimation method for random sonic fatigue life of thin-walled structure of a combustor liner based on stress probability distribution

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航空动力学报 2011年第4期26卷 727-734页

作者： sha Yun-dong GUO Xiao-peng LIAO Lian-fang XIE Li-juan Liaoning Key Laboratory of Digital Technology Simulation and Test Technique College of Power and Energy Engineering Shenyang Aerospace University Shenyang 110136 China Shenyang Aeroengine Research Institute Aviation Industry Corporation of China Shenyang 110015 China

As to the sonic fatigue problem of an aero-engine combustor liner structure under the random acoustic loadings,an effective method for predicting the fatigue life of a structure under random loadings was ***,the probability distribution of Von Mises stress of thin-walled structure under random loadings was studied,analysis suggested that probability density function of Von Mises stress process accord approximately with two-parameter Weibull *** formula for calculating Weibull parameters were *** on the Miner linear theory,the method for predicting the random sonic fatigue life based on the stress probability density was developed,and the model for fatigue life prediction was *** an example,an aero-engine combustor liner structure was *** power spectrum density(PSD) of the vibrational stress response was calculated by using the coupled FEM/BEM(finite element method/boundary element method) model,the fatigue life was estimated by using the constructed *** considering the influence of the wide frequency band,the calculated results were *** analysis shows that the estimated results of sonic fatigue of the combustor liner structure by using Weibull distribution of Von Mises stress are more conservative than using Dirlik distribution to some *** results show that the methods presented in this paper are practical for the random fatigue life analysis of the aeronautical thin-walled structures.

关键词： thin-walled structure sonic fatigue life estimation probability density function(PDF) power spectrum density(PSD)

Thermal comfort assessment in civil aircraft cabins

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Chinese Journal of Aeronautics 2014年第2期27卷 210-216页

作者： Pang Liping Qin Yue Liu dong Liu meng School of Aviation Science and Engineering Beihang University

Aircraft passengers are more and demanding in terms of thermal comfort. But it is not yet easy for aircraft crew to control the environment control system （ECS） that satisfies the thermal comfort for most passengers due to a number of causes. This paper adopts a corrected predicted mean vote （PMV） model and an adaptive model to assess the thermal comfort conditions for 31 investigated flights and draws the conclusion that there does exist an uncomfortable thermal phe- nomenon in civil aircraft cabins, especially in some short-haul continental flights. It is necessary to develop an easy way to predict the thermal sensation of passengers and to direct the crew to con- trol ECS. Due to the assessment consistency of the corrected PMV model and the adaptive model, the adaptive model of thermal neutrality temperature can be used as a method to predict the cabin optimal operative temperature. Because only the mean outdoor effective temperature ET＊ of a departure city is an input variable for the adaptive model, this method can be easily understood and implemented by the crew and can satisfy 80-90% of the thermal acceptability levels of passen- gers.

关键词： Adaptive model of thermalneutrality temperature Civil aircraft cabin Mean outdoor effectivetemperature Optimal operativetemperature PM~ Thermal comfort assessment