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表达绿色荧光蛋白的乳酸杆菌在鸡胃肠道中的分布

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免疫学杂志 2007年第2期23卷 126-130页

作者：庾庆华董莎萌朱伟云杨倩南京农业大学农业部动物生理生化重点开放实验室南京210095

目的构建含有绿色荧光蛋白基因(green fluorescent protein gene,gfp)的质粒,电转化从鸡胃肠中分离的乳酸杆菌,并口服接种小鸡表达绿色荧光蛋白(green fluorescent protein,GFP)的重组乳酸杆菌,观察该乳酸杆菌在胃肠道中的分布。方法在... 详细信息

目的构建含有绿色荧光蛋白基因(green fluorescent protein gene,gfp)的质粒,电转化从鸡胃肠中分离的乳酸杆菌,并口服接种小鸡表达绿色荧光蛋白(green fluorescent protein,GFP)的重组乳酸杆菌,观察该乳酸杆菌在胃肠道中的分布。方法在获得稳定表达绿色荧光蛋白的德氏乳杆菌乳亚种D17(D17-GFP)菌株后,口服接种20日龄的鸡,于接种后1.5、6、12、24、48和72h分别取胃肠内容物接种培养并计数,同时取胃、空肠、回肠、盲肠、肝和脾脏做冰冻切片在荧光显微镜下观察D17-GFP的分布。结果D17-GFP在接种鸡6h后,胃肠道的内容物中可发现D17-GFP,在42h时细菌数达到最大,并且3d后菌量仍维持在1×104CFU/g左右。在荧光显微镜下发现D17-GFP主要存在于肠道的内容物和黏液中。结论GFP在德氏乳杆菌乳亚种D17中得到了稳定的表达,重组D17-GFP可在鸡胃肠道的黏膜处定植。

关键词：乳酸杆菌绿色荧光蛋白胃肠道分布

Progress of research in the application of ultrasound technology for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第10期20卷 2823-2837页

作者： Qiuquan Cai Lianghui meng Meina Quan Ling Wang Jing Ren Chenguang Zheng Jiajia Yang dong Ming Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjinChina Innovation Center for Neurological Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Brain-Computer Interaction and Human-Machine Fusion Haihe Laboratory Tianjin UniversityTianjinChina Tianjin Key Laboratory of Brain Science and Neural Engineering Tianjin UniversityTianjinChina

Alzheimer’s disease is a common neurodegenerative disorder defined by decreased reasoning abilities,memory loss,and cognitive *** presence of the blood-brain barrier presents a major obstacle to the development of effective drug therapies for Alzheimer’s *** use of ultrasound as a novel physical modulation approach has garnered widespread attention in recent *** a safe and feasible therapeutic and drug-delivery method,ultrasound has shown promise in improving cognitive *** article provides a summary of the application of ultrasound technology for treating Alzheimer’s disease over the past 5 years,including standalone ultrasound treatment,ultrasound combined with microbubbles or drug therapy,and magnetic resonance imaging-guided focused ultrasound *** is placed on the benefits of introducing these treatment methods and their potential *** found that several ultrasound methods can open the blood-brain barrier and effectively alleviate amyloid-βplaque *** believe that ultrasound is an effective therapy for Alzheimer’s disease,and this review provides a theoretical basis for future ultrasound treatment methods.

关键词： Alzheimer’s disease blood-brain barrier drugs magnetic resonance imaging-guided focused ultrasound microbubbles scanning ultrasound ultrasound ultrasound stimulation

High-resolution imaging through dynamic scattering media: Iterative optimization based on polarimetric characteristics

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Science China Technological Sciences 2025年第1期 82-84页

作者： meng XIANG Kai DENG Tianyu WANG Jinpeng LIU Xue dong Teli XI Fei LIU Xiaopeng shaO School of Optoelectronic Engineering Xidian University Xi'an Key Laboratory of Computational Imaging National Key Laboratory of Infrared Detection Technologies

High-resolution imaging through randomly dynamic scattered fields and highly scattered walls is an extensively sought-after capability with potential applications in various fields such as underwater imaging, biomedical imaging, and seeing through fog. Numerous methods have been proposed to unscramble object information from degraded scattered images, resulting in considerable improvements in image contrast in degraded scenarios [1].

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Enhanced solid-electrolyte interface efficiency for practically viable hydrogen-air fuel cell systems

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Journal of Energy Chemistry 2025年第1期100卷 356-368页

作者： Venkitesan Sakthivel dong Jin Yoo Graduate School Department of Energy Storage/Conversion Engineering (BK21 FOUR) Hydrogen and Fuel Cell Research Center Jeonbuk National University Jeonju Department of Life Science Jeonbuk National University

Proton exchange membrane fuel cells （PEMFCs） provide an appealing sustainable energy system,with the solid-electrolyte membrane playing a crucial role in its overall ***,sulfonated poly（1,4-phenylene ether-ether sulfone）(SPEES),an aromatic hydrocarbon polymer,has garnered considerable attention as an alternative to Nafion ***,the long-term durability and stability of SPEES present a significant *** this context,we introduce a potential solution in the form of an additive,specifically a core–shell-based amine-functionalized iron titanate （A–Fe2TiO5）,which holds promise for improving the lifetime,proton conductivity,and power density of SPEES in *** modified SPEES/A–Fe2TiO5composite membranes exhibited notable characteristics,including high water uptake,enhanced thermomechanical stability,and oxidative ***,the SPEES membrane loaded with 1.2 wt%of A–Fe2TiO5demonstrates a maximum proton conductivity of 155 mS cm-1,a twofold increase compared to the SPEES membrane,at 80°C under 100%relative humidity （RH）.Furthermore,the 1.2 wt%of A–Fe2TiO5/SPEES composite membranes exhibited a maximum power density of 397.37 mW cm-2and a current density of 1148 mA cm-2at 60°C under 100%RH,with an opencircuit voltage decay of 0.05 m V/h during 103 h of continuous *** study offers significant insights into the development and understanding of innovative SPEES nanocomposite membranes for PEMFC applications.

关键词： Acid-base Hybrid membrane Proton conductivity Polymer electrolyte fuel cell Single-cell performance

Small modifications of endo-functionalized cavities lead to large changes in molecular interaction in water

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Science China Chemistry 2025年第1期 369-376页

作者： Song-meng Wang Hao Nian Yan-Fang Wang Li-Shuo Zheng Yu-Tao Zheng Yi-Wei dong Liping Huang Xiaoping Wang Wei Jiang Liu-Pan Yang Department of Chemistry Southern University of Science and Technology School of Chemistry and Pharmaceutical Sciences State Key Laboratory for Chemistry and Molecular Engineering of Medicinal ResourcesGuangxi Normal University School of Pharmaceutical Science Hengyang Medical School University of South China

Studying molecular recognition in aqueous environments is crucial for understanding biological processes. Herein, we present a series of N-doped endo-functionalized anthracene-based cavities by introducing inward-directed pyridine units. The subtle modifications can significantly affect the binding properties of these receptors, which is similar to the mutations in biological systems. Combining theoretical calculations and molecular recognition experiments demonstrated that these “mutations” have an impact on the hydrophobicity and charge distribution of the hosts, consequently significantly influencing molecular *** is crucial for understanding the intricate mechanisms of molecular-level interactions in biological receptors.

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Relative contribution of genetic and environmental factors to determination of wing morphs of the brown planthopper Nilaparvatalugens

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Insect Science 2023年第1期30卷 208-220页

作者： Chao Zhang meng-sha Mao Xiang-dong Liu Department of Entomology Nanjing Agricultural UniversityNanjingChina

Wing dimorphism is a fascinating feature of the ability of insects to adapt to *** brown planthopper(BPH)Nilaparvata lugens,a serious pest of rice,can switch between the long-and short-winged *** has been known that environmental factors can affect the wing morph of ***,it is still unclear whether the effect of environment is dependent on BPH genetic backgrounds or *** the present study,we established the pure-bred lineages of short-and long-winged BPHs via multigenerational selection,and we found that survival and fecundity were similar between these 2 *** morphs of the pure-bred lineages were almost fully dependent on genetics,but independent of the environmental factors,nymphal density and rice plant stage,2 key factors affecting BPH wing *** the unselected BPH population,short-and longwinged morphs were produced depending on those 2 environmental factors,indicating the contribution of environment to wing *** the wing-selected lineages,4 developmental regulated genes of wing,NllnRI,NllnR2,NIAkt,and NIFoxo were expressed stably in the short-winged adults,but almost silenced in the long-winged ***,all these genes were expressed normally with a similar level in both the short-and long-winged adults in an unselected population except *** pure-bred lineages of long-and short-winged morphs exhibited different expression patterns of wing development-regulated genes,suggesting the genetic determination of wing *** of environmental factors on wing morphs occurred only in the genetic mix population.

关键词： environmental effect gene expression genetic determination Nilaparvata lugens pure-bred lineage wing dimorphism

Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong Chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

An Artificial Intelligence-Assisted Flexible and Wearable Mechanoluminescent Strain Sensor System

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NANO-MICRO LETTERS 2025年第1期17卷 1-15页

作者： dong, Yan An, Wenzheng Wang, Zihu Zhang, dongzhi China Univ Petr East China Coll Control Sci & Engn Qingdao 266580 Peoples R China

The sandwich-structured flexible mechanoluminescent sensor (SFLC) film shows great application potential as wireless wearable strain sensor and encryption ***-level integration of SFLC film with deep learning-based artificial intelligence enables fast and accurate interpretation of color data to strain values with automatic correction of errors caused by varying color *** smart glove wearable sensor based on the SFLC film combined with deep learning neural network enables fast and accurate hand gesture recognition. The complex wiring, bulky data collection devices, and difficulty in fast and on-site data interpretation significantly limit the practical application of flexible strain sensors as wearable devices. To tackle these challenges, this work develops an artificial intelligence-assisted, wireless, flexible, and wearable mechanoluminescent strain sensor system (AIFWMLS) by integration of deep learning neural network-based color data processing system (CDPS) with a sandwich-structured flexible mechanoluminescent sensor (SFLC) film. The SFLC film shows remarkable and robust mechanoluminescent performance with a simple structure for easy fabrication. The CDPS system can rapidly and accurately extract and interpret the color of the SFLC film to strain values with auto-correction of errors caused by the varying color temperature, which significantly improves the accuracy of the predicted strain. A smart glove mechanoluminescent sensor system demonstrates the great potential of the AIFWMLS system in human gesture recognition. Moreover, the versatile SFLC film can also serve as a encryption device. The integration of deep learning neural network-based artificial intelligence and SFLC film provides a promising strategy to break the "color to strain value" bottleneck that hinders the practical application of flexible colorimetric strain sensors, which could promote the development of wearable and flexible strain sensors from laboratory research to

关键词： Mechanoluminescent Strain sensor Flexible Deep learning Wireless

5,7,2',4',5'-Pentamethoxyflavanone regulates M1/M2 macrophage phenotype and protects the septic mice

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Chinese Journal of Natural Medicines 2019年第5期17卷 363-371页

作者： FENG Li-Li XU Li-sha GUO meng-meng HUANG Wei ZHU Jia-Zhen KONG Ling-dong WU Xu-dong XU Qiang State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University

Flavonoids have been reported to exert protective effect against many inflammatory diseases, while the underlying cellular mechanisms are still not completely known. In the present study, we explored the anti-inflammation activity of 5, 7, 2', 4', 5'-pentamethoxyflavanone(abbreviated as Pen.), a kind of polymethoxylated flavonoid, both in vitro and in vivo experiments. Pen. was showed no obvious toxicity in macrophages even at high dosage treatment. Our results indicated that Pen. significantly inhibited both mR NA and protein level of proinflammatory cytokines, IL-1β, IL-6, TNF-α and iN OS, which was characteristic expressed on M1 polarized macrophages. These effects of Pen. were further confirmed by diminished expression of CD11c, the M1 macrophage surface marker. Further researches showed that the mechanism was due to that Pen. downregulated the activity of p65, key transcription factor for M1 polarization. On the other hand, Pen. also enhanced M2 polarization with upregulation of anti-inflammatory factors and increase of M2 macrophage surface markers, which lead to the balance of M1 and M2 macrophages. Moreover, in vivo research verified that Pen. treatment alleviated LPS-induced sepsis in mice by increasing survival rate, decreasing inflammatory cytokines and improving lung tissue damage. In summary, our results suggested that Pen. modulated macrophage phenotype via suppressing p65 signal pathway to exert the anti-inflammation activity.

关键词： Macrophage polarization Anti-inflammation NFκB Sepsis 5,7,2',4',5'-Pentamethoxyflavanone(Pen.)