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Urgent one-stage endoscopic treatment for choledocholithiasis related moderate to severe acute cholangitis: A propensity scorematched analysis

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World Journal of Gastroenterology 2024年第15期30卷 2118-2127页

作者： yang Zhou Yin-Qiu Zhang Shuai-Jing Huang yan Liang Xiao Liang Masoom Wali ya-dong feng Department of Gastroenterology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210009Jiangsu ProvinceChina Department of Gastroenterology BenQ Medical CenterThe Affiliated BenQ Hospital of Nanjing Medical UniversityNanjing 210019Jiangsu ProvinceChina

BACKGROUND During emergency endoscopic retrograde cholangiopancreatography(ERCP),the safety and feasibility of performing one-stage endoscopic treatment for patients with acute cholangitis(AC)due to choledocholithiasis are *** To investigate the safety and feasibility of one-stage endoscopic treatment for moderate to severe *** We enrolled all patients diagnosed with moderate to severe cholangitis due to common bile duct stones from January 2019 to July *** outcomes were compared in this study between patients who underwent ERCP within 24 h and those who underwent ERCP 24 h later,employing a propensity score(PS)*** primary outcomes were intensive care unit(ICU)admission rates,ICU length of stay,and duration of antibiotic *** In total,we included 254 patients and categorized them into two groups based on the time elapsed between admission and intervention:The urgent group(≤24 h,n=102)and the elective group(>24 h,n=152).Ninety-three pairs of patients with similar characteristics were selected by PS *** urgent ERCP group had more ICU admissions(34.4%vs 21.5%,P=0.05),shorter ICU stays(3 d vs 9 d,P<0.001),fewer antibiotic use(6 d vs 9 d,P<0.001),and shorter hospital stays(9 d vs 18.5 d,P<0.001).There were no significant differences observed in adverse events,in-hospital mortality,recurrent cholangitis occurrence,30-d readmission rate or 30-d *** Urgent one-stage ERCP provides the advantages of a shorter ICU stay,a shorter duration of antibiotic use,and a shorter hospital stay.

关键词： Acute cholangitis Endoscopic retrograde cholangiopancreatography One-stage treatment Optimal time

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MRI影像组学无创预测脑胶质母细胞瘤MGMT启动子甲基化状态的价值

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放射学实践 2024年第4期39卷 449-454页

作者：马千昂卢俊董亚锋曲金荣郑州大学附属肿瘤医院/河南省肿瘤医院医学影像科河南郑州450003 首都医科大学北京友谊医院放射科北京100050

目的:探讨MRI影像组学模型术前预测脑胶质母细胞瘤(GBM)O 6-甲基鸟嘌呤-DNA甲基转移酶启动子甲基化(MGMT-PM)状态的价值。方法:回顾性分析2018年1月-2021年10月在本院经病理证实的130例脑GBM患者的临床资料和MRI图像(ADC和对比增强3D-T ... 详细信息

目的:探讨MRI影像组学模型术前预测脑胶质母细胞瘤(GBM)O 6-甲基鸟嘌呤-DNA甲基转移酶启动子甲基化(MGMT-PM)状态的价值。方法:回顾性分析2018年1月-2021年10月在本院经病理证实的130例脑GBM患者的临床资料和MRI图像(ADC和对比增强3D-T 1WI)。其中,MGMT-PM阳性组(PM率≥8%)58例,MGMT-PM阴性组(PM率<8%)72例。按7:3的比例将所有患者随机分为训练集(91例)和验证集(39例)。由2位放射科医师独立在ADC和CE-3D-T 1WI图像上逐层勾画ROI,获得病灶的全域容积感兴趣区(VOI),分别提取851个组学特征。然后,采用最小绝对收缩和选择算法(LASSO)进行特征降维,将保留下来的特征与其对应的系数进行线性组合,构建影像组学模型并计算每例患者的影像组学评分(Radscore),得到Radscore ADC、Radscore CE-T 1WI和Radscore联合三组评分。采用ROC曲线评估各组学模型的诊断效能,将最优模型的Radscore和临床特征(年龄、性别)纳入logistic回归分析构建预测MGMT-PM状态的临床-组学综合模型,并绘制其诺模图。采用ROC曲线评价综合模型的预测效能,并采用校准曲线和决策曲线分别评估此模型的校准度和临床实用价值。结果:在训练集中,Radscore联合预测MGMT-PM状态的AUC为0.872,优于单一序列(Radscore ADC:AUC=0.798,P<0.05;Radscore CE-T 1WI:AUC=0.840,P<0.05);在验证集中得到了一致的结论。在影像组学模型中加入临床特征后,可提高预测效能,临床-组学综合模型的AUC、敏感度和特异度分别为0.904、92.50%和78.43%。校准曲线显示临床-组学综合预测模型在训练集和验证集中预测概率与实际概率之间的差异均无统计学意义(P=0.051、0.284)。决策曲线分析表明综合预测模型具有一定的临床实用价值。结论:MRI影像组学模型有助于术前无创性预测GBM的MGMT启动子甲基化状态,多序列结合及引入临床特征能提高模型的预测效能。

关键词：脑肿瘤胶质母细胞瘤 MGMT启动子甲基化磁共振成像影像组学

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Elimination of grapevine fleck virus and grapevine rupestris stem pitting-associated virus from Vitis vinifera 87-1 by ribavirin combined with thermotherapy

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Journal of Integrative Agriculture 2021年第9期20卷 2463-2470页

作者： HU Guo-jun dong ya-feng ZHANG Zun-ping FAN Xu-dong REN Fan Research Institute of Pomology Chinese Academy of Agricultural SciencesXingcheng 125100P.R.China

Vitis vinifera 87-1 plants infected by grapevine fleck virus(GFkV) and grapevine rupestris stem pitting-associated virus(GRSPaV) were used as the plant materials for virus elimination treatment. This study evaluated the effects of ribavirin at different concentrations(15 and 25 μg mL^(–1);R15 and R25, respectively), thermotherapy(37°C;T), and the combination of ribavirin and thermotherapy(R15+T and R25+T) on eliminating viruses from grapevine plants in vitro. Both R15 and R25 had phytotoxic effects and weakened plant growth. Thermotherapy positively affected the growth of grapevine plants. Plant height was significantly greater in T, R15+T, and R25+T than in CK, R15 and R25. The proportion of dead plants after T, R15+T, and R25+T was 51.4, 11.4, and 8.6%, respectively. The survival rates of regenerated plants after all treatments were >68.0%. Ribavirin concentration and treatment time were related to the regeneration of shoot tips and elimination efficiencies of the two viruses. The survival rates of plants after R15+T for 30, 40, and 50 days were 97.3, 90.7, and 74.4%, respectively. The elimination rates of GRSPaV from plants in the three time quantum were 55.6, 84.6, and 93.8%, respectively. The elimination rate of GFkV was 23.9% higher in R25(35/44) than in R15(25/45), and that of GRSPaV was 7.0% higher in R25 than in R15. The combination of thermotherapy and chemotherapy was found to have a positive effect on the eradication of GFkV and GRSPaV, and R25+T for 50 days was able to completely eliminate the two viruses from in vitro grapevines.

关键词： in vitro grapevine grapevine fleck virus(GFk V) grapevine rupestris stem pitting-associated virus(GRSPaV) chemotherapy thermotherapy virus elimination

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Molecular characterization of Apple necrotic mosaic virus identified in crabapple(Malus spp.) tree of China

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Journal of Integrative Agriculture 2019年第3期18卷 698-701页

作者： HU Guo-jun dong ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang Research Institute of Pomology Chinese Academy of Agricultural Sciences

Apple necrotic mosaic virus(ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction(RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple(ApNMV-Hai) and two isolates from apple(ApNMV-Hua and-Qu) were determined. The sizes of genomic RNA1, 2 and 3 of the three isolates differed from those of the previously reported isolate ApNMV-P126 from Japanese apple, especially RNA3. Compared with the nucleotide(nt) sequence of RNA3 in isolate P126, those in the Hai and Qu isolates were 7 and 33 nt shorter, respectively, and that of isolate Hua was 7 nt longer. Alignment analyses showed that these differences in size were mainly due to differences in the lengths of the 5′ untranslated region(UTR) and the UTR region between the ORFs encoding the movement protein and the coat protein. In the phylogenetic trees constructed using the full genomic sequences of RNA1, 2 and 3, the isolate Hai clustered into a group with the isolate Qu in the RNA1 tree, but formed an individual branch in the RNA2 and 3 trees. Three recombination events were identified in the nucleotide sequences of RNA1 and 2 among the isolates ApNMV-Hai,-Hua, and-Qu. This is the first report of the full genome sequence of ApNMV in crabapple.

关键词： crabapple mosaic symptom Apple necrotic mosaic virus molecular characterization

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Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China

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Journal of Integrative Agriculture 2020年第3期19卷 768-774页

作者： FAN Xu-dong ZHANG meng-yan ZHANG Zun-ping REN Fang HU Guo-jun dong ya-feng National Center for Eliminating Viruses from Deciduous Fruit Tree Research Institute of PomologyChinese Academy of Agricultural Sciences

A total of 288 grapevine samples of 61 different grapevine cultivars,collected from 22 provinces and regions,were analyzed by reverse transcription-PCR(RT-PCR) for the presence of grapevine fabavirus(GFabV).PCR detection results showed the incidences of GFabV were 12.8%(30/235) and 48.1%(25/52) in the asymptomatic and symptomatic vines,*** genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein *** analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined *** 1,2,and 4 comprised only Chinese *** article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China.

关键词： GFabV RT-PCR polyprotein gene phylogenetic analysis

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Hepatic microenvironment underlies fibrosis in chronic hepatitis B patients

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World Journal of Gastroenterology 2020年第27期26卷 3917-3928页

作者： Qun-yan yao ya-dong feng Pei Han feng yang Guang-Qi Song Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 201332China Shanghai Institute of Liver Diseases Shanghai 201332China Otsuka Shanghai Research Institute Shanghai 201318China Department of Pancreatic Surgery Pancreatic Disease InstituteHuashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China

BACKGROUND Chronic hepatitis B virus(HBV)infection is a leading cause of liver morbidity and mortality *** fibrosis resulting from viral infection-associated inflammation and direct liver damage plays an important role in disease management and *** mechanisms underlying the contribution of the liver microenvironment to fibrosis in HBV patients are not fully *** is an absence of effective clinical treatments for liver fibrosis progression;thus,establishing a suitable in vitro microenvironment in order to design novel therapeutics and identify molecular biomarkers to stratify patients is urgently *** To examine a subset of pre-selected microenvironment factors of chronic HBV patients that may underlie fibrosis,with a focus on fibroblast *** We examined the gene expression of key microenvironment factors in liver samples from patients with more advanced fibrosis compared with those with less severe *** also used the human stellate cell line LX-2 in the in vitro *** different recombinant cytokines and growth factors or their combination,we studied how these factors interacted with LX-2 cells and pinpointed the crosstalk between the aforementioned factors and screened the most important *** Of the secreted factors examined,transforming growth factor(TGF)-β1,interleukin(IL)-1βand tumor necrosis factor(TNF)-αwere increased in patients with advanced *** found that besides TGF-β1,IL-1βcan also induce a profibrotic cascade by stimulating the expression of connective tissue growth factor and platelet-derived growth factor(PDGF)in LX-2 ***,the proinflammatory response can be elicited in LX-2 cells following treatment with IL-1βand TNF-α,suggesting that stellate cells can respond to proinflammatory *** combining IL-1βand TGF-β1,we observed not only fibroblast activation as shown byαlpha-smooth muscle actin and PDGF induction,but also the inflammatory response

关键词： Microenvironment Liver fibrosis Chronic hepatitis B Human stellate cell Interleukin-1β

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A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types

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Journal of Integrative Agriculture 2020年第7期19卷 1834-1841页

作者： REN Fang ZHANG Zun-ping FAN Xu-dong HU Guo-jun ZHANG Meng-yan dong ya-feng National Center for Eliminating Viruses from Deciduous Fruit Trees Research Institute of PomologyChinese Academy of Agricultural SciencesXingcheng 125100P.R.China

To develop a rapid and high-sensitivity method for detection of grapevine virus E(GVE),a SYBR Green based real-time fluorescence quantitative RT-PCR method(RT-qPCR)was *** method could be used to detect GVE specifically,and the sensitivity was about 100 times greater than conventional *** excellent linear correlation(R=0.997)and a high amplification efficiency(E=97.5%)were obtained from the standard curve of this *** tests revealed that the coefficients of variation in the intra-and inter-assay results were 0.31-1.03%and 0.82--262%,respectively,indicating a good *** RT-qPCR method could be used to detect GVE in a wide range of grapevine sample *** detection rates of RT-qPCR for nearly all sample types from different positions and seasons were higher than conventional *** detection rates in spring,summer,autumn and winter increased *** in autumn and winter were best for detection,and the detection rates of most samples were 80-100%,which were 10 to 40%higher than conventional *** general,old petioles and branches were the best tissues for GVE *** detection rates of these samples in each season were all 100%,which were 20 to 40%higher than conventional *** second highest rates were in the old leaf,with detection rates for RT-qPCR of 80-100%in all seasons,which were 20 to 40%higher than conventional *** could be difficultly detected in young leaves by conventional RT-PCR,and the detection rates were only 0-50%,while by RT-qPCR the rates could increase to 0--80%.A total of 33 out of 363 samples(belonging to 68 cultivars)from 20 regions in China were detected to be positive by RT-qPCR(9.1%),which was more than twice the rate of the conventional RT-PCR(3.9%).

关键词： grapevine grapevine virus E detection RT-qPCR conventional RT-PCR

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Effect of pre-culture on virus elimination from in vitro apple by thermotherapy coupled with shoot tip culture

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Journal of Integrative Agriculture 2018年第9期17卷 2015-2023页

作者： HU Guo-jun dong ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang LI Zheng-nan Research Institute of Pomology Chinese Academy of Agricultural Sciences

We evaluated the role of pre-culture on survival rate of in vitro apple plants treated by thermotherapy. Two apple cultivars, Malusxdomestica cv. Pink Lady and Huafu, were used in the experiment and both have widely grown in China and infected with Apple chlorotic leafspot virus （ACLSV） and Apple stem grooving virus （ASGV）. Results in growth and virus titer of apple plants did not exhibit clear trends during five different periods of pre-culture. Whilst, pre-culture increased the survival rate of the two cultivars during thermotherapy. The survival rate of plants pre-cultured for 13 d （P-13d） was 14 and 51% higher than that of P-ld plants for Pink Lady and Huafu, respectively. Moreover, pre-culture positively influenced regeneration of Huafu plants. The average survival rate of plants regenerated from P-ld and P-4d was 20% lower than that regenerated from P-7d, P-10d, and P-13d. The efficiency of virus eradication was determined by reverse-transcription PCR with two primer pairs for each virus, and the detection results showed that pre-culture scarcely affected apple virus elimination. Despite the fact that the two viruses were hardly detected at 5 d of thermotherapy, no virus-free plants were found in the two cultivars of regenerated apple plantlets after 30-d treatment.

关键词： apple in vitro culture pre-culture thermotherapy virus elimination

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Molecular Chain Flexibility and Dielectric Loss at High-Frequency:Impact of Ester Bond Arrangement in Poly(ester imide)s

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Chinese Journal of Polymer Science 2024年第8期42卷 1122-1133页

作者： ya-dong Li Hong Li Lu-Kun feng feng Bao Ming-Liang Wang Cai-Zhen Zhu Zhao-Hui Zheng Xiao-Bin Ding Jian Xu Chengdu Institute of Organic Chemistry Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100081China Institute of Low-Dimensional Materials Genome Initiative College of Chemistry and Environmental EngineeringShenzhen UniversityShenzhen 518060China

The evolution of high-frequency communication has accentuated the significance of controlling dielectric properties in polymer ***,it has been theorized that rigid molecular chains lead to lower dielectric ***,the validity of this proposition at high frequencies remains *** scrutinize the correlation between chain flexibility and dielectric properties,we synthesized six poly(ester imide)s(PEIs)with systematically varied molecular chain flexibilities by modifying the ester's substitution on the aromatic *** introduction of ester bonds bestowed all PEI films with a low dielectric dissipation factor(D_(f)),ranging from 0.0021 to 0.0038 at 10 GHz in dry *** dry D_(f)displayed a pattern consistent with volume polarizability(P/V).Unexpectedly,PI-mmm-T,featu ring the most flexible molecular chain,exhibited the lowest dielectric loss under both dry(0.0021@10 GHz)and hygroscopic(0.0029@10 GHz)***,the observed increase in D_(f)after humidity absorption suggests that the high dielectric loss of PEI in applications may be attributed to its hygroscopic *** simulations and characterization of the aggregation structure revealed that the smaller cavities within flexible molecular chains,after close stacking,impede the entry of water *** sacrificing high-temperature resistance,the precursor exhibited enhanced solubility properties and could be processed into high-quality *** research unveils new insights into the relationship between flexibility and highfrequency dielectric loss,offering innovative perspectives on synthesizing aromatic polymers with exceptional dielectric properties.

关键词： 5G communications Poly(ester imide)s Molecular chain flexibility Dielectric properties Humidity absorption

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Mechanical performance of 22SiMn2TiB steel welded with lowtransformation- temperature filler wire and stainless steel filler wire

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Journal of Iron and Steel Research International 2024年第4期31卷 967-981页

作者： Zi-dong Lin Kai-jie Song Zhen Sun Zi-qian Zhu Xue-feng Zhao Constantinos Goulas Wei ya Xing-hua Yu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Design Production and Management Faculty of Engineering Technology.University of TwenteEnschede 7500 AETheNetherlands Rotterdam Additive Manufacture Fieldlab(RAMLAB) Rotterdam 3089 JWTheNetherlands

TX-80 low-transformation-temperature(LTT)welding wire was used to replace the traditional ER 307Si welding wire to realize the connection of 22SiMn2TiB armor steel in manual overlay *** previously existing issues,such as welding cracks,large welding deformation,and severe welding residual stress,were solved to ensure good strength and ductility *** particular,with the same welding conditions,TX-80 LTT wire eliminates welding *** reduces the welding deformation no matter the base pretreatment of pre-setting angle or no pre-setting *** comparison,it was found that the microstructure at the TX-80 weld is mainly composed of martensite and a small amount of retained *** contrast,the microstructure of the ER 307Si weld consists of a large amount of austenite and a small amount of skeleton-like *** variation trend of residual stress and microhardness from the weld to the base were investigated and compared with the mechanical properties of base *** TX-80 and the ER 307Si tensile samples elongation is 6.76%and 6.01%,while the ultimate tensile strengths are 877 and 667 MPa,*** average impact toughness at room temperature of the ER 307Si weld is 143.9 J/cm^(2),much higher than that of the TX-80 weld,which is only 36.7 J/cm^(2).The relationship between impact and tensile properties with microstructure species and distribution was *** addition,the fracture surface of the tensile and the impact samples was observed and *** dimples,fewer pores,larger radiation zone,and shear lips of TX-80 samples indicate better tensile ductility and worse impact toughness than those of ER 307Si weld.

关键词： Low-transformation-temperature material 22SiMn2TiB steel Welding deformation Residual stress Martensitic transformation Mechanical property

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