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In Vitro Angiogenic Behavior of HUVECs on Biomimetic SF/SA Composite Scaffolds

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Journal of Wuhan University of Technology(Materials Science) 2021年第3期36卷 456-464页

作者： KOMBO Omar Ramadhan WANG Xinyu SHEN Ying LIU Jiawei dong Xianzhen SHAO Qi LONG Yanpiao dong kuo BAKHET Shahd Fateh El-Rahman Elkhider LI Binbin State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Biomedical Materials and Engineering Research Center of Hubei Province Wuhan 430070China

The 60Fc and 70Fc SF/SA blend scaffolds were prepared to mimic the functions of the native ECM for skin *** Umbilical Vein Endothelial Cells(HUVECs)were used to examine the cell cytotoxicity,adhesion,growth factors secretion and the gene expression of associated angiogenic *** proliferation,adhesion and live-dead analyses showed that HUVECs could better attach,grow,and proliferate on the 70Fc scaffolds compared with 60Fc scaffolds and unmodified ***,the 70Fc scaffolds showed higher levels of specific angiogenic proteins and genes expression as *** study suggests that the involvement of higher composition of SF(about 70%)than that of SA on the blended scaffolds could be advantageous as it is more suitable to promote angiogenesis,which is potential for vascularization during skin repair.

关键词： vascularization scaffold HUVECs tissue engineering

Novel variants in DNAH6 cause male infertility associated with multiple morphological abnormalities of the sperm flagella(MMAF)and ICSI outcomes

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Asian Journal of Andrology 2024年第1期26卷 91-98页

作者： Zhong-Mei Shao Yu-Tong Zhu Meng Gu Sen-Chao Guo Hui Yu kuo-kuo Li dong-dong Tang Yu-Ping Xu Ming-Rong Lv Reproductive Medicine Center Department of Obstetrics and Gynecologythe First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Department of Obstetrics and Gynecology Fuyang Hospital of Anhui Medical UniversityFuyang 236112China NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract(Anhui Medical University) Hefei 230032China Key Laboratory of Population Health Across Life Cycle(Anhui Medical University) Ministry of Education of the People'sRepublicof ChinaHefei 230032China

Variations in the dynein axonemal heavy chain gene,dynein axonemal heavy chain 6(DNAH6),lead to multiple morphological abnormalities of the *** studies have reported that these deficiencies may result in sperm head ***,whether DNAH6 is also involved in human acrosome biogenesis remains *** purpose of this study was to investigate DNAH6 gene variants and their potential functions in the formation of defective sperm heads and ***-exome sequencing was performed on a cohort of 375 patients with asthenoteratozoospermia from the First Affiliated Hospital of Anhui Medical University(Hefei,China).Hematoxylin and eosin staining,scanning electron microscopy,and transmission electron microscopy were performed to analyze the sperm morphology and *** staining and Western blot analysis were conducted to examine the effects of genetic *** identified three novel deleterious variants in DNAH6 among three unrelated *** absence of inner dynein arms and radial spokes was observed in the sperm of patients with DNAH6 ***,deficiencies in the acrosome,abnormal chromatin compaction,and vacuole-containing sperm heads were observed in these patients with DNAH6 *** decreased levels of the component proteins in these defective structures were further confirmed in sperm from patients with DNAH6 variants using Western *** intracytoplasmic sperm injection(ICSl)treatment,the partner of one patient with a DNAH6 variant achieved successful ***,novel variants in DNAH6 genes that contribute to defects in the sperm head and flagella were identified,and the findings indicated Icsl as an effective clinical treatment for such patients.

关键词： asthenoteratospermia DNAH6 intracytoplasmic sperm injection male infertility

Immobilization of cross-linked In-doped Mo(O,S)2 on cellulose nanofiber for effective organic-compound degradation under visible light illumination

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Progress in Natural Science:Materials International 2021年第3期31卷 404-413页

作者： Hairus Abdullah Chi-Ning Hsu Hardy Shuwanto Noto Susanto Gultom Worku Lakew Kebede Chang-Mou Wu Chiu-Chun Lai Ri-Ichi Murakami Masayuki Hirota Antonio Norio Nakagaito dong-Hau kuo Department of Materials Science and Engineering National Taiwan University of Science and Technology Department of Textile Engineering Chinese Culture University New Material Research & Development Department Frontier Materials Laboratories Osaka Gas Chemicals Co. Ltd. Graduate School of Technology Industrial and Social Sciences Tokushima University

Relatively small amounts of In-doped Mo(O,S)2(IMS) catalysts(10%, 20%, and 30%) were deposited on cellulose nanofiber(CNF) by cross-linking them with functional groups of siloxane and epoxy to form CNF-IMS hybrid composites. The as-prepared hybrid composites were characterized and tested their performances toward the photo degradations of cationic(MB and Rh B) and anionic(MO) dyes. As indium was doped into Mo(O,S)2lattice to form solid-solution, the charge transfer and photocarrier separation during the catalytic reaction were simultaneously enhanced as probed with electrochemical impedance spectroscopy and photoluminescence measurements, respectively. To ensure the catalyst on CNF was well deposited and recyclable, the hybrid composite was evaluated with a reusability experiment to show the stability performance in degrading organic dyes. It was found the environmentally friendly CNF-IMS hybrid composite was relatively stable during the reusability experiment, indicating no catalyst powder was leached out during the photocatalytic reaction. The photoreaction mechanism was convinced by radical-scavenging experiments to show that hydroxyl and superoxide played essential roles for the organic dye degradations in visible-light illuminated conditions. The cross-linked organic/inorganic hybrid catalyst showed a prospective visible light active material not only to solve the environmental issue due to the leaching of nanoparticles but also to lower the post-treatment/recycling cost of photocatalyst in industrial application.

关键词： Cellulose nanofiber In-doped Mo(O,S) 2 Cross-linking In 2 (O,S) 3 Organic dye degradation

Natural variation of GmFNSII-2 contributes to drought resistance by modulating enzyme activity in soybean

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The Crop Journal 2024年第2期12卷 529-539页

作者： Huihui Gao Pengcheng Wei Yongzhe Gu Pengbin Tang Yifan Shen Lei Yang Linxin dong Haowei Zheng kuo Shu Mayamiko Masangano Bin dong Long Miao Jiajia Li Lijuan Qiu Xiaobo Wang School of Agronomy Anhui Agricultural UniversityHefei 230036AnhuiChina State Key Laboratory of Crop Gene Resources and Breeding The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

As an essential crop that provides vegetable oil and protein,soybean(Glycine max(L.)Merr.)is widely planted all over the ***,the scarcity of water resources worldwide has seriously impacted on the quality and yield of *** address this,exploring excellent genes for improving drought resistance in soybean is *** this study,we identified natural variations of GmFNSII-2(flavone synthase II)significantly affect the drought resistance of *** sequence analysis of GmFNSII-2 in 632 cultivated and 44 wild soybeans nine haplotypes were *** full-length allele GmFNSII-2^(C),but not the truncated allele GmFNSII-2^(A) possessing a nonsense nucleotide variation,increased enzyme *** research found that GmDREB3,known to increase soybean drought resistance,bound to the promoter region of GmFNSII-2^(C).GmDREB3 positively regulated the expression of GmFNSII-2^(C),increased flavone synthase abundance and improved the drought ***,a singlebase mutation in the GmFNSII-2^(C) promoter generated an additional drought response element(CCCCT),which had stronger interaction strength with GmDREB3 and increased its transcriptional activity under drought *** frequency of drought-resistant soybean varieties with Hap 1(Pro:GmFNSII-2^(C))has increased,suggesting that this haplotype may be selected during soybean *** summary,GmFNSII-2^(C) could be used for molecular breeding of drought-tolerant soybean.

关键词： Drought stress Flavone synthase GmFNSII-2 Natural variation Drought response element

Effect of Temperature Dependence on Electrical Characterization of <i>p-n</i>GaN Diode Fabricated by RF Magnetron Sputtering

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Materials Sciences and Applications 2015年第9期6卷 809-817页

作者： Thi Tran Anh Tuan dong-Hau kuo Cheng-Che Li Guan-Zhang Li Department of Materials Science and Engineering National Taiwan University of Science and Technology Taiwan School of Basic Sciences Tra Vinh University Tra Vinh Vietnam

The p-n junction GaN diodes were all fabricated by sputtering technique with cermet targets for p- and n-type GaN and metal targets for electrodes. The interface of these p-n junction GaN diodes examined by high-resolution transition electron microscopy was clear and distinguishable. Lattice images identified the complete dissolution of Mg into the Ga site. At the room temperature, the diode had the turn-on voltage of 2.2 V, the leakage current of 2.2 × 10–7 A, the breakdown voltage of –6 V, the barrier height of 0.56 eV, ideality factor of 5.0 by I (current)-V (voltage) test and 5.2 derived from the Cheungs’ method, and series resistance of 560 Ω. These electrical properties were investigated at different testing temperatures from room temperature to 200°C. The temperature dependence in the I-V characteristics of the p-n diodes can be successfully explained on the basis of thermionic-emission mode.

关键词： p-n GaN Diode I-V Measurement Barrier Height Leakage Current Cheungs’ Method

Material and Device Developments of p/n Ⅲ-Nitrde Thin Films made by Low-Temperature Sputtering

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Material and Device Developments of p/n Ⅲ-Nitrde Thin Films...

振动场下FFF热熔喷头内熔体表观黏度的理论研究

第二届海峡两岸功能材料科技与产业峰会（2015）

作者： dong-Hau kuo Thi Tran Anh Tuan Cheng-Che Li Wei-Chun Yen Department of Materials Science and Engineering "National Taiwan University of Science and Technology"

GaN and In GaN layers have been deposited by metal-organic vapor deposition(MOCVD)and molecular beam *** MOCVD,In GaN films grown at 550-900 oC use trimethylindium(TMIn),trimethylgallium(TMGa),and NH as reaction precursors and N as carrier *** high capital cost and process temperature and the utilization of dangerous precursors are the major concerns of MOCVD process.p-Ga N films grown at 1000

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东北大学学报（自然科学版） 2021年第3期42卷 395-400页

作者：姜世杰陈丕峰孙明宇董天阔东北大学机械工程与自动化学院辽宁沈阳110819

热熔喷头内部熔体的表观黏度是影响熔丝制造成型(FFF)产品机械性能的关键因素之一.本文首先提出利用振动改善FFF热熔喷头内部熔体表观黏度的方法,即对喷头施加纵向的正弦振动力场;然后针对施加振动后热熔喷头内部熔体的表观黏度进行了... 详细信息

热熔喷头内部熔体的表观黏度是影响熔丝制造成型(FFF)产品机械性能的关键因素之一.本文首先提出利用振动改善FFF热熔喷头内部熔体表观黏度的方法,即对喷头施加纵向的正弦振动力场;然后针对施加振动后热熔喷头内部熔体的表观黏度进行了理论建模,并利用已有文献中的实验结果验证了理论模型的正确性;最后,利用所建模型探讨了施加不同频率振动后熔体表观黏度的变化规律,阐明了相应机理.研究表明:熔体表观黏度的理论与实验结果吻合度较好,理论模型正确;对热熔喷头施加振动场后,熔体表观黏度有效值随着振动频率的增大而逐渐降低.

关键词：熔丝制造成型热熔喷头振动表观黏度理论模型

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论短波通信在消防救援中的重要作用

数字技术与应用 2020年第8期38卷 38-39,42页

作者：董括天津市消防救援总队天津301600

在实际的消防救援活动中,消防应急通信技术与救援效率具有直接联系,其在救援过程中发挥着十分重要的作用。本文首先分析了短波通信的传播方式及原理,阐述了消防救援中短波通信的重要作用,论述了消防救援中短波通信的具体运用,以供参考。

关键词：短波通信消防救援

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一种基于共享度模型的改进Rete算法

自动化学报 2017年第9期43卷 1571-1579页

作者：孙新严西敏尚煜茗欧阳童董阔北京理工大学计算机学院北京市海量语言信息处理与云计算应用工程技术研究中心北京100081

专家系统是人工智能领域的重要分支,其中知识表示和知识推理是专家系统的重要组成部分.Rete算法是一种高效的模式匹配算法,能够解决专家系统中推理效率的问题,但是Rete算法在构建Rete网络和推理过程中存在空间和性能方面问题.本文采取... 详细信息

专家系统是人工智能领域的重要分支,其中知识表示和知识推理是专家系统的重要组成部分.Rete算法是一种高效的模式匹配算法,能够解决专家系统中推理效率的问题,但是Rete算法在构建Rete网络和推理过程中存在空间和性能方面问题.本文采取有穷自动机理论的思想,阐述了Rete算法中的模式共享度和节点共享度模型,提出了一种Rete网络构建和推理算法来降低Rete网络的复杂度,提升Rete网络推理的速度.最后实验结果表明,本算法能够降低网络复杂度,提升推理速度.

关键词：专家系统 Rete算法有穷自动机推理机