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The Relation Between Endogenous Hormones and Late-Germination in Buds of Avrolles Apple

Agricultural Sciences in China 2009年第5期8卷 564-571页

作者： QIN dong WANG Jin-zheng GUO jian-min ZHAI Heng State Key Laboratory of Crop Biology/College of Horticulture Science and Engineering Shandong Agricultural University Tai＇an 271018 P.R.China Shandong Research Institution of Pomology Tai＇an 271000 P.R. China

In order to provide the physiological bases for selecting late-germination cultivars that can avoid late frost damage, the very late-germination variety Avrolles （Malus domestica） was used to study the relation between the dynamic changes and balance of endogenous hormones and germination time. The concentrations of endogenous GA3, ABA, IAA, and ZR were determined in buds of Avrolles and Judeline （Malus domestica） from dormancy releasing to germination by capillary electrophoresis. The dynamic changes of endogenous hormones concentration in buds of Avrolles and Judeline were similar; but the magnitude and time of the change were significantly different between the two varieties, especially for GA3. GA3 concentration increased with dormancy releasing, then decreased, and increased again before germination in the two varieties. GA3 concentration in Avrolles was 1.72 times that in Judeline at the first peak, the gap increased to 2.22 times at germination. ZR concentration exhibited a continuous increase trend, but it decreased sharply before germination. ZR accumulation in Avrolles took 36 days longer than in Judeline, the peak value was 44% higher than in Judeline. Before germination, ZR concentration in Avrolles was 2.12 times that in Judeline. The differences between IAA and ABA concentration were relatively small in the two varieties, while the ratios of GA3/ABA and （GA3 ＋ IAA ＋ ZR）/ABA in Avrolles were 2.08 and 1.58 times those in Judeline, respectively. The germination of apple bud was regulated by the endogenous hormones. For the late-germination apple Avrolles, its germination requires higher concentration of GA3 and ZR, which leads to the high ratios of GA3/ABA and （GA3＋ IAA＋ ZR）/ABA.

关键词： Malus domestica cv. Avrolles Malus domestica cv. Judeline late-germination endogenous hormone

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Bi同位素链上原子核α衰变性质的理论研究

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原子核物理评论 2010年第2期27卷 136-139页

作者：王永佳张鸿飞王艳召董建敏蔡凌志李君清左维兰州大学核科学与技术学院甘肃兰州730000 中国科学院近代物理研究所甘肃兰州730000

采用结团模型(clustermodel)计算了从10752Te到292116共443个核素α衰变的半衰期,所得结果与实验值符合得很好,显示了结团模型可以成功地应用于研究原子核α衰变的性质。同时研究了Bi同位素链上奇A核α衰变的半衰期,计算结果与已有的实... 详细信息

采用结团模型(clustermodel)计算了从10752Te到292116共443个核素α衰变的半衰期,所得结果与实验值符合得很好,显示了结团模型可以成功地应用于研究原子核α衰变的性质。同时研究了Bi同位素链上奇A核α衰变的半衰期,计算结果与已有的实验值的偏差一般在3倍以内,进而对实验上未知α衰变半衰期的原子核的性质进行了预言。这有助于将来在实验室探测与鉴别这些原子核以及研究它们的α衰变性质。理论与实验的比较将加深人们对原子核结构的认识。

关键词：结团模型 α衰变半衰期

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杓唇石斛化学成分研究

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中成药 2022年第11期44卷 3517-3521页

作者：杨宗玉张瑜杨进罗婉丽张茂生王刚孙成新董敏健肖世基遵义医科大学药学院贵州遵义563000

目的研究杓唇石斛Dendrobium moschatum(Buch.-Ham.)Sw.的化学成分。方法杓唇石斛90%甲醇提取物运用各种柱色谱和制备液相色谱分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离纯化得到11个化合物,分别为香豆素(1)、... 详细信息

目的研究杓唇石斛Dendrobium moschatum(Buch.-Ham.)Sw.的化学成分。方法杓唇石斛90%甲醇提取物运用各种柱色谱和制备液相色谱分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离纯化得到11个化合物,分别为香豆素(1)、2,5-二羟基-4-甲氧基-9,10-二氢菲(2)、4-甲氧基菲-2,5-二醇(3)、ozoroalide(4)、(9 Z,11E)-13-羟基-9,11-十八碳二烯酸(5)、5-羟基-3-甲氧基-联苄(6)、4,4′-二羟基-3,3′,5-三甲氧基联苄(7)、山药素Ⅲ(8)、邻羟基苯丙酸甲酯(9)、邻羟基苯丙酸乙酯(10)、2-乙酰氧基苯丙酸甲酯(11)。结论化合物2~11为首次从杓唇石斛中分离得到,其中化合物4、5、9、10为首次从兰科植物中分离得到,化合物11为新的天然产物。

关键词：杓唇石斛化学成分结构鉴定

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Suxiao Jiuxin Pills Prevent Ventricular Fibrillation from Inhibiting L-type Calcium Currents CaV1.2 in vivo and in vitro

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Chinese Journal of Integrative Medicine 2023年第2期29卷 108-118页

作者： QI jian-yong KANG dong-yuan YU Juan ZHANG min-zhou Acute Myocardial Infarction Key Laboratory of Chinese Medicine in Guangzhou the 2nd Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Province Hospital of Chinese MedicineGuangzhou 510006China Intensive Care Research Team of Traditional Chinese Medicine the 2nd Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Province Hospital of Chinese MedicineGuangzhou 510006China Animal Center the 2nd Affiliated Hospital of Guangzhou University of Chinese MedicineGuangdong Province Hospital of Chinese MedicineGuangdong Province Academy of Chinese MedicineGuangzhou 510006China

Objective: To investigate whether Suxiao Jiuxin Pills(SJP), a Chinese herbal remedy, is an anti-ventricular fibrillation(VF) agent. Methods: VF was induced by isoproterenolol(ISO) intraperitoneal injection followed by electrical pacing in mice and rabbits. The effects of SJP on the L-type calcium channel current(CaV1.2), voltage-dependent sodium channel current(INa), rapid and slow delayed rectifier potassium channel current(IKr and IKs, respectively) were studied by whole-cell patch-clamp method. Computer simulation was implemented to incorporate the experimental data of SJP effects on the CaV1.2 current into the action potential(AP) and pseudo-electrocardiography(pseudo-ECG) models. Results: SJP prevented VF induction and reduced VF durations significantly in mice and rabbits. Patch-clamp experiments revealed that SJP decreased the peak amplitude of the CaV1.2 current with a half maximal concentration(IC50) value of 16.9 mg/L(SJP-30 mg/L, –32.8±6.1 pA;Verapamil, –16.2±1.8 pA;vs. control, –234.5±16.7 pA, P0.05). Computer simulation showed that SJP-reduced CaV1.2 current shortened the AP duration,transiting VF into sinus rhythm in pseudo-ECG. Conclusion: SJP reduced VF via inhibiting the CaV1.2 current with in vivo, in vitro, and in silico studies, which provide experimental basis for SJP anti-VF clinical application.

关键词： Suxiao Jiuxin Pills ventricular fibrillation patch clamp simulation CaV1.2

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Pharmacological targeting cGAS/STING/NF-κB axis by tryptanthrin induces microglia polarization toward M2 phenotype and promotes functional recovery in a mouse model of spinal cord injury

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Neural Regeneration Research 2025年第11期20卷 3287-3301页

作者： Ziwei Fan Mengxian Jia jian Zhou Zhoule Zhu Yumin Wu Xiaowu Lin Yiming Qian Jiashu Lian Xin Hua jianhong dong Zheyu Fang Yuqing Liu Sibing Chen Xiumin Xue Juanqing Yue minyu Zhu Ying Wang Zhihui Huang Honglin Teng Department of Orthopedics(Spine Surgery) the First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina School of Pharmacy Hangzhou Normal UniversityHangzhouZhejiang ProvinceChina Department of Neurology The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Pathology Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina Department of Clinical Research Center Affiliated Hangzhou First People’s HospitalSchool of MedicineWestlake UniversityHangzhouZhejiang ProvinceChina

The M1/M2 phenotypic shift of microglia after spinal cord injury plays an important role in the regulation of neuroinflammation during the secondary injury phase of spinal cord *** of shifting microglia polarization from M1(neurotoxic and proinflammatory type)to M2(neuroprotective and anti-inflammatory type)after spinal cord injury appears to be *** possesses an anti-inflammatory biological ***,its roles and the underlying molecular mechanisms in spinal cord injury remain *** this study,we found that tryptanthrin inhibited microglia-derived inflammation by promoting polarization to the M2 phenotype in *** promoted M2 polarization through inactivating the cGAS/STING/NF-κB ***,we found that targeting the cGAS/STING/NF-κB pathway with tryptanthrin shifted microglia from the M1 to M2 phenotype after spinal cord injury,inhibited neuronal loss,and promoted tissue repair and functional recovery in a mouse model of spinal cord ***,using a conditional co-culture system,we found that microglia treated with tryptanthrin suppressed endoplasmic reticulum stress-related neuronal *** together,these results suggest that by targeting the cGAS/STING/NF-κB axis,tryptanthrin attenuates microglia-derived neuroinflammation and promotes functional recovery after spinal cord injury through shifting microglia polarization to the M2 phenotype.

关键词： cGAS/STING functional recovery microglia neuroinflammation neuroprotection nuclear factor-κB polarization spinal cord injury tryptanthrin

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基于摆锤法的成熟期油菜角果炸荚因素试验分析

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农机化研究 2025年第4期47卷 180-185页

作者：李港张敏魏德欣江东农业农村部南京农业机械化研究所南京210014

为了降低油菜联合收割机拨禾轮拨禾过程对角果打击造成的炸荚损失,采用摆锤法模拟拨禾轮拨禾对油菜角果冲击过程,对影响拨禾过程中的炸荚因素进行测试分析,并对角果损伤变化形式及过程进行分析。试验结果表明:摆锤冲击速度3.5 m/s、夹... 详细信息

为了降低油菜联合收割机拨禾轮拨禾过程对角果打击造成的炸荚损失,采用摆锤法模拟拨禾轮拨禾对油菜角果冲击过程,对影响拨禾过程中的炸荚因素进行测试分析,并对角果损伤变化形式及过程进行分析。试验结果表明:摆锤冲击速度3.5 m/s、夹持果柄位置距连接点2 mm是区分角果抗打击炸荚的最优参数;油菜角果炸荚不仅与冲击速度、果柄夹持位置有关,而且与油菜品种、冲击件材料类型、角果受力面、含水率、角果在植株上的分布位置等都有关系;冲击速度不超过1.5 m/s时,角果损伤以角果果柄发生塑性变形为主;冲击速度为2.5~4.5 m/s时,角果损伤以果皮破裂分离、果柄弹性变形为主。研究结果可为油菜拨禾轮设计和工作参数选择提供参考。

关键词：油菜角果炸荚率冲击拨禾轮摆锤法

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Measurements of Wind Loads on Side-by-Side Semi-Submersibles in A Wind Tunnel

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China Ocean Engineering 2021年第4期35卷 531-543页

作者： dong Qing GUO Xiao-xian YANG jian-min LU Hai-ning HUANG Longwei State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University(SJTU)Shanghai 200240China SJTU Yazhou Bay Institute of Deepsea Technology Sanya 572000China

The multi-body system has been a popular form for offshore operations in terms of high *** wind effects are crucial which directly affect the relative positions of floating bodies and operating *** this study,the aerodynamic characteristics for two coupled semi-submersibles were analyzed in a wind tunnel to fill the gaps in literature related to the wind sheltering on offshore *** influences of separation distance were also *** to the results,substantial shielding effects were observed and wind forces on the shielded vessel decreased dramatically:a reduction in the transverse force could be up to 74%.Moreover,the longitudinal wind load was amplified by the platform abreast in a side-by-side *** expected,the interference level became more pronounced with a decreasing separation *** cases in which wind interaction decayed rapidly with distance,logarithmic functions were preferable for describing the relationship between *** linear fitting was reasonable for the transverse wind force when there was still evident sheltering at a quite large *** length of shielding area was another important factor that there was approximately a linear relationship between it and the shielding level for two platforms in close proximity at various wind attack *** on the two parameters,a preliminary wind loads estimation method considering shielding effects was *** approach can aid the industry to have a qualitative assessment of wind sheltering especially at early stages.

关键词： wind loads side-by-side shielding effect wind tunnel test

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左乳内动脉旁路内逆向血流的影响因素研究

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山西医科大学学报 2005年第5期36卷 620-623页

作者：赵向东姚建民马旭东杨碧波北京军区总医院心肺血管中心一区北京100700 北京大学第三医院心脏外科

目的研究冠状动脉旁路移植术(CABG)中左前降支(LAD)自身狭窄程度、有效侧支循环及左室前壁及前间壁有无活动异常对左乳内动脉(LIMA)旁路内逆向血流(RF)形成的影响。方法对CABG手术治疗中建立LIMA-LAD旁路的133例患者的LIMA旁路进行分析... 详细信息

目的研究冠状动脉旁路移植术(CABG)中左前降支(LAD)自身狭窄程度、有效侧支循环及左室前壁及前间壁有无活动异常对左乳内动脉(LIMA)旁路内逆向血流(RF)形成的影响。方法对CABG手术治疗中建立LIMA-LAD旁路的133例患者的LIMA旁路进行分析。按3种不同影响因素对比分成6组,测量各组患者LIMA-LAD旁路平均RF速度,分析诸因素对RF形成的各自影响程度。结果狭窄程度不同的两组间RF速度平均值统计学差异显著(P0.05)。结论来自LAD近端的自身血流可显著影响CABG术后LIMA-LAD旁路的瞬时血流灌注,是旁路内产生RF的重要因素,LAD有效侧支循环的建立也是RF的主要成因之一。RF与左室前壁及前间壁有无活动异常无明确相关性。

关键词：冠状动脉旁路移植术乳内动脉逆向血流

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罗氏沼虾幼苗体内一种极小病毒的序列测定与分析

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武汉大学学报（理学版） 2004年第6期50卷 736-740页

作者：张化俊袁军法曹铮钱冬王建民石正丽中国科学院武汉病毒研究所分子病毒学实验室湖北武汉430071 浙江省淡水水产研究所浙江湖州313001

从患罗氏沼虾幼苗肌肉白浊病的幼虾体内分离到两种病毒颗粒,诺达病毒(MrNV)和直径只有15nm的小病毒颗粒(extrasmallvirus,XSV),本文通过建立病毒cDNA文库获得XSV基因组的部分序列,在此基础上合成探针,用从病虾组织中提取的总PNA进行Nort... 详细信息

从患罗氏沼虾幼苗肌肉白浊病的幼虾体内分离到两种病毒颗粒,诺达病毒(MrNV)和直径只有15nm的小病毒颗粒(extrasmallvirus,XSV),本文通过建立病毒cDNA文库获得XSV基因组的部分序列,在此基础上合成探针,用从病虾组织中提取的总PNA进行Northern杂交,结果表明XSV基因组至少包括两条RNA片段.利用不同的方法对XSV的两端序列进行克隆和测定,分别得到了872、831和662bp3个相互重叠的序列片段,这3个序列的阅读框编码一个相同的大小在17×103左右的蛋白.

关键词：罗氏沼虾肌肉白浊病极小病毒序列测定

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聚碳酸酯用耐磨透明涂料的应用研究

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现代涂料与涂装 2018年第9期21卷 4-6页

作者：魏超王志强顾剑东周敏施颖波杨阳中昊北方涂料工业研究设计院有限公司兰州730020

以正硅酸乙酯(TEOS)、甲基三乙氧基硅烷(MTES)、硅溶胶、异丙醇等为主要原材料,通过溶胶-凝胶方法制备了聚碳酸酯表面透明耐磨涂料。通过探究硅烷单体的选择及用量,附着力促进剂的用量对涂层性能的影响,溶剂、助剂的选择及固化条件的影... 详细信息

以正硅酸乙酯(TEOS)、甲基三乙氧基硅烷(MTES)、硅溶胶、异丙醇等为主要原材料,通过溶胶-凝胶方法制备了聚碳酸酯表面透明耐磨涂料。通过探究硅烷单体的选择及用量,附着力促进剂的用量对涂层性能的影响,溶剂、助剂的选择及固化条件的影响,最终制备了可应用于聚碳酸酯表面防护的透明耐磨涂料。

关键词：溶胶-凝胶透明耐磨附着力涂料

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