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An aqueous extract of Liu Wei Di Huang Wan alters the labeling of blood constituents with Technetium-99m

Chinese Science Bulletin 2013年第18期58卷 2061-2065页

作者： MATOS Vernica Soares de CARMO Fernanda Santos do diniz claudia leite NASCIMENTO Ana Lucia Rosa PEREIRA Márcia Oliveira FERNANDES Joelma Fonseca de Oliveira SANTOS-FILHO Sebastiao David MORENO Silvana Ramos Farias BERNARDO-FILHO Mario Departamento de Biofísica e Biometria Instituto de Biologia Roberto Alcantara GomesUniversidade do Estado do Rio de Janeiro

The Liu Wei Di Huang Wan is a formula of a traditional Chinese medicine that is used to treat asthma patients and has been shown to have several important properties,such as antioxidant and free radical scavenging *** influence of an extract of Liu Wei Di Huang Wan on the labeling of blood constituents with technetium-99m was *** blood(Wistar rats) was incubated with the extract,stannous chloride and technetium-99m,as sodium *** were centrifuged and aliquots of plasma and blood cells were separated and precipitated with trichloroacetic acid,to obtain soluble and insoluble fractions of the blood *** percentage of radioactivity(%ATI) in all the fractions was *** analysis of the results shows that the extract at the highest concentration used(70 mg/mL) decreased significantly(P<0.05) the %ATI(from 96.48 ± 1.19 to 54.46 ± 7.38) on blood cells compartment,(from 81.11 ± 4.15 to 61.33 ± 4.74) on insoluble fractions of blood cells and(from 65.91 ± 2.44 to 13.15 ± 3.62) on insoluble fractions of *** conclusion,the results suggest that the substances present on this extract can alter this labeling process,probably due to(i) redox properties(antioxidant and chelator activities) and/or(ii) specific actions in the binding sites where the 99mTc would be bound on the blood *** a consequence,precaution is suggested on the interpretation of the nuclear medicine results from performed with blood constituents labeled with 99m Tc in patients that have undertaken LWDHW,although the current findings were obtained in experimental animal models.

关键词：六味地黄丸血液成分标签水提物 Wistar大鼠氧化还原性能锝实验动物模型

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Nursing Diagnostics of Nutrition Domain of NANDA International in Hemodialysis Patients

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Open Journal of Nursing 2016年第3期6卷 204-212页

作者： Érida Maria diniz leite Sama Mikaella de Oliveira Maria Isabel da Conceição Dias Fernandes Maria das Graças Mariano Nunes Cyndi Fernandes de Lima Ana Luisa Brandão de Carvalho Lira Department of Nursing Hospital Academic Onofre Lopes Natal Brasil Department of Nursing University of Rio Grande do Norte Natal Brasil

Introduction: The dialysis causes the loss of the nutrients and the elevation of catabolism, thus, patients in dialysis have at high risk for the loss of body reserves of protein and energy. Objective: To identify nursing diagnoses of nutrition domain of NANDA International in hemodialysis patients and to correlate them to the socioeconomic and clinical data. Design: Cross-sectional study. Setting: Performed in a large university hospital in northeastern Brazil. Participants: The first stage was carried out with 50 patients, selected with the following inclusion criteria: Being hospitalized and undergoing hemodialysis in that hospital;aged 18 years old or over;being conscious and oriented. Exclusion criteria were patients undergoing hemodialysis in that hospital with external service, pregnant women and patients being treated by plasmapheresis. In the second stage, participants were three nursing specialists in nephrology and nursing diagnoses. Methods: The defining characteristics of the nursing diagnoses inserted in the nutrition domain were observed in patients and then these data were judged by nurses, in order to identify diagnoses present in each patient. The Chi-square, Fisher’s exact and Mann-Whitney tests were used in the analysis of the relationship of socioeconomic and clinical data, with an adopted p value of p = 0.001), origin (p = 0.014), religion (p = 0.046) and income (p = 0.039), respectively. Conclusion: The variables that showed significant association were risk of impaired liver function and gender;imbalanced nutrition: More than body requirements and origin requirements;risk for deficient fluid volume and religion;and deficient fluid volume and income.

关键词： Nursing Nursing Diagnosis Risk Groups Nutrition Renal Dialysis

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Aquatic Phoretic Mites (Acari: Hydrachnidia) Associated with Ectoparasitism of Mosquitoes (Diptera: Culicidae) in the Midwest Region of Brazil

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Advances in Entomology 2016年第3期4卷 141-150页

作者： Fábio Alexandre Leal dos Santos Sirlei Frank Thies Aldimara Vaillant Gonçalves Klaucia Rodrigues Vasconcelos Monaly da Silva Ribeiro Junio de Souza Damasceno Elisangela Santana de Oliveira Dantas diniz Pereira leite Júnior Medical Entomology Laboratory Faculty of Medicine Federal University of Mato Grosso UFMT Cuiabá Brazil University Center of Várzea Grande (UNIVAG) Cristo Rei Várzea Grande Brazil Pontifical Catholic University of Minas Gerais Postgraduate Program in Vertebrate Biology Conservation Genetics Laboratory Belo Horizonte Brazil University Center of Belo Horizonte Institute of Engineering and Technology (IET) Belo Horizonte Brazil Laboratory of Investigation Faculty of Medicine Federal University of Mato Grosso UFMT Cuiabá Brazil

Water mites (Hydrachnidia) are common external parasites of the medically important mosquitoes (Culicidae). Between April 2014 and April 2015, 64 mites from three genus, Arrenurus 71.9%, Limnochares 3.1%, and Hydrachna 25.0%, were collected from female mosquitoes in two rural locations near Cuiabá, Mato Grosso, Brazil. Dipterous species parasitized by water mites belonging to seven species: Anopheles (Nys.) darling, An. evansae, Aedes (Och.) scapularis, Ae. serratus, Mansonia (Man.) wilsoni, Psorophora (Jan.) ferox, Ps. varipes. The most common specimens to accommodate the water mites were Anopheles (Nys.) darlingi and Psorophora (Jan.) varipes. The prevalence of parasitism of mosquitoes by water mites found in this study was less than 5. However, few studies have addressed the ecological role of mites and their biotopes, as well as host-parasite interactions in Brazil.

关键词： Ectoparasitism Hydracarina Culicidae Mites

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Indoor Air Mycological Survey and Occupational Exposure in Libraries in Mato Grosso-Central Region—Brazil

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Advances in Microbiology 2018年第4期8卷 324-353页

作者： diniz Pereira leite Júnior Ronaldo Sousa Pereira Washington Santos de Almeida Sara de Almeida Alves Simoes Ana Caroline Akeme Yamamoto Janaina Vasconcellos Ribeiro de Souza Evelin Rodrigues Martins Fábio Alexandre Leal dos Santos Rosane Christine Hahn Faculty of Medicine Post Graduate Course in Health Sciences-Federal University of Mato Grosso(UFMT)CuiabáMTBrazil Specialized Medical Mycology Center Federal University of Mato Grosso(UFMT)CuiabáMTBrazil University Center of Várzea Grande(UNIVAG) Várzea GrandeMGBrazil Faculty of Medicine of Sao Josédo Rio Preto(FAMERP) Sao PauloSPBrazil

Background: Indoor air quality in environments where there is great circulation of people, posing risks to the health of its occupants, including allergic problems, infections and contaminations, can be aided by climatic factors, chemicals and biological agents housed in these environments, influencing the location and providing favorable conditions for the degradation of bibliographic collections. The present study investigated the presence of fungi in indoor environments in seven public and private libraries in the central region of Brazil, Mato Grosso, and verified the impact on occupational health. Results: A total of 26,194 fungal specimens were isolated from 342 dust samples collected using three techniques: Andersen’s sampler (12.3%), exposure plate dish (25.1%) and sterile swab (62.6%). A total of 184 fungal species were identified: 156 (84.8%) mycelial fungi and 28 (15.2%) yeast fungi, belonging 54 fungal genera, 43 (79.6%) mycelial fungi and 11 (20.4%) yeast fungi. The genus Aspergillus (40.6%) was one of the main fungi present in indoor air. Aspergillus niger (12.3%) was identified as the most prevalent species in literary environments, followed by Cryptococcus spp. (7.1%) and Cladosporium cladosporioides (7.0%). In relation to seasonal distribution, there was a greater fungal isolation in the dry season (54%);followed by the rainy season (46%). Conclusion: These results suggest the substrates researched in the evaluated environments presented in the form of documents, books and papers associated with dust and air humidity become suitable for microbiological proliferation. These findings highlight the importance of minimizing the risk of exposure to fungal agents, identified in pathogenic and toxigenic microenvironments in library collections.

关键词： Biological Hazards Library Collections Anemophilous Fungi Indoor Air Quality

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Challenges of the Global COVID-19 Pandemic and Invasive Fungal Pathogens in SARS-COV-2 Associations: A Dangerous Relationship

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Advances in Microbiology 2023年第1期13卷 1-23页

作者： Alessandra Gomes Mariscal Beatriz Fernanda Siqueira Matias Cíntia Batista de Almeida Nadir Cléia Alves de Santana Simone Matos de Campos Roseli de Souza Klaucia Rodrigues Vasconcelos Margareth Léa da Silva Maia Cristiane Coimbra de Paula diniz Pereira leite Júnior Faculdade EducareMT Cuiab&aacute Brazil Centro Universitá rio de Vá rzea Grande (UNIVAG) V&aacute rzea Grande Brazil Laborató rio Central de Saú de Pú blica do Estado de Mato Grosso (LACEN) Cuiab&aacute Brazil Instituto Ciê ncias Biomé dicas (ICB) Universidade de Sã o Paulo (USP) S&atilde o Paulo Brazil Laboratory Carlos Chagas Sabin Group Cuiab&aacute Brazil Instituto Adolfo Lutz Center of Parasitology and Micology Micology Nucleus S&atilde o Paulo Brazil Research Laboratory Medical School Universidade Federal de Mato Grosso (UFMT) Cuiab&aacute Brazil

Background: COVID-19 is a rapidly transmissible pneumonia-like illness caused by SARS-CoV-2 that out broke in China in 2019 and is currently circulating worldwide. In the current context of the SARS-CoV-2 pandemic, complications are observed in clinical settings for the treatment of severe COVID-19 disease in nosocomial settings, due to cases of fungal co-infections. Objective: To carry out a review on fungal infections associated with respiratory infections caused by COVID-19 (Sars-Cov-2) and their aggravation. Methodology: The purpose of this study is to inform the reader about the characteristics of SARS-CoV-2 and the main fungal species that are affecting patients undergoing treatment for severe COVID-19, provoking discussion of the importance of the proposed topic, in relation to co-infections by different fungal microorganisms. Result: 80 scientific studies were selected, resulting from patients with COVID-19 and most commonly observed in patients with a history of comorbidities such as diabetes mellitus, hypertension, kidney disease, severe liver disease, oncological diseases, obesity and with severe COVID-19. These data do not represent the total number of records of the disease in the world, but cases reported by researchers in their series, showing the overlapping of fungal co-infection through the compromised immune status due to the use of therapeutic drugs, dysregulation of the microbiota, age of patients and the severity itself of the severe inflammation caused by COVID-19. Conclusion: The immunosuppression caused by the infection of COVID-19 concomitant with its therapy through corticosteroid therapy and comorbidities of the patients made fungal infections more susceptible, and these interfere in the evolution of the case and in the treatment of COVID-19, being relevant to distinguish secondary infections to therapy and the best possible reconstitution of the clinical picture.

关键词： Fungal Coinfections COVID-19 Coronavirus Systematic Review

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Cryptococcus and Cryptococcosis in Human and Animal Model:An Overview

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Advances in Microbiology 2020年第4期10卷 180-193页

作者： Eriques Goncalves da Silva diniz Pereira leite-Jr Margareth Léa da Silva Maia Sandra Marilia de Souza Silva Francisco de Assis Baroni Marcos Ereno Auler Amanda Latércia Tranches Dias Débora Moreira Rinaldo Ferreira Gandra Regina Teixeira Barbieri Ramos André Gasparetto Elisangela Santana de Oliveira Dantas Valéria Tognato Costa Claudete Rodrigues Paula School of Dentistry University of Sao Paulo(USP)Sao PauloBrazil Specialized Medical Mycology Center Federal University of Mato Grosso(UFMT)CuiabáBrazil Federal University of ABC(UFABC) Santo AndréBrazil Rural Federal University of Rio de Janeiro(UFRRJ) Rio de JaneiroBrazil Faculty of Pharmacy University of the Western Center of Paraná(UNICENTRO)Campus CedetegGuarapuavaBrazil Federal University of Alfenas(UNIFAL) AlfenasBrazil University Hospital State University of West Paraná(UNIOESTE)CascavelBrazil Department of Dentistry State University of Maringa(UEM)MaringáBrazil Institute of Biosciences Federal University of Mato Grosso(UFMT)CuiabáBrazil

Background: Yeasts from the genus Cryptococcus (species C. neoformans and C. gattii) are opportunistic pathogens that afflict humans with low immunity caused by diseases such as AIDS or by the usage of immunosuppressive drugs. It is a fatal disease that has been studied around the whole world and its most fatal form is the brain cryptococcosis. Purpose: The present review describes the disease from pathogen isolation, the various clinical presentations of the disease, the most important virulence factors of yeast in human and animal model and their clinical issues. Methods: On this review, several published studies about the disease are presented. Results: Numerous researches have been done worldwide in order to find a kind of therapy that is more effective against the disease. Amphotericin B, in all forms is still the drug of choice in the treatment of the cryptococcosis. Fluconazole, as well as voriconazole in combination with amphotericin B, is recommended in the cases of treatment failure. Conclusion: This study presented has elucidated a little more about the disease. Further studies should be conducted to find more diagnoses that are accurate as well as more effective treatments for eradicating the disease. In this study, the bibliographic survey makes reference to the world literature;with regard to ecology, taxonomy, mains factors related to virulence, the clinical manifestations, the action of antifungal drugs and histopathological analysis used in an animal model, were the objectives deleterious aspects of this study, thus informing, in a simple way, the importance of this microorganism for research and researchers working with this global disease, called Cryptococcosis.

关键词： Cryptocococcus Cryptococcosis Animal Model Cryptococcus neoformans Cryptococcusgattii Virulence Related Factors

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Characterization of Clinical and Environmental Isolates of <i>Cryptococcus neoformans</i>/<i>Cryptococcus gattii</i>Complex Maintained in Yeast Culture Collection in São Paulo, Brazil

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Open Journal of Epidemiology 2018年第2期8卷 76-92页

作者： Pedro Henrique Magalhã es Cardoso Bosco Christiano Maciel da Silva Jairo Ivo dos Santos Rennan Luiz Oliveira dos Santos diniz Pereira leite Jú nior Marcos Ereno Auler Luciana da Silva Ruiz Eriques Gonç alves da Silva Dé bora Moreira Carina Domaneschi Francisco de Assis Baroni Marcia de Souza Carvalho Melhem Marilena dos Anjos Martins Claudete Rodrigues Paula Faculty of Veterinary Medicine and Zootechny-FMVZ-USP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Faculty of Medicine/FMUSP University of S&#227 o Paulo (USP) S&#227 o Paulo Brazil Federal University of Santa Catarina (UFSC) Florian&oacute polis Brazil Faculty of Dentistry University of S&#227 o Paulo (FOUSP) S&#227 o Paulo Brazil Faculty of Medicine Federal University of Mato Grosso (UFMT) Cuiab&aacute Brazil Faculty of Pharmacy University of the Western Center of Paran&aacute (UNICENTRO) Guarapuava Brazil Institute Adolfo Lutz (IAL) Bauru Brazil Federal Rural University of Rio de Janeiro (UFRRJ) Serop&eacute dica Brazil Institute Adolfo Lutz (IAL) S&#227 o Paulo Brazil

Objective: As isolates of Cryptococcus are frequently kept collection stocks in institutions, sometimes without proper characterization, we sought to determine the genotype profiles, protease and phospholipase activities “in vitro” and the susceptibility testing for azoles and amphotericin B. Methodology: 84 isolates from several regions of Brazil (40 samples from clinical origin and 44 isolates from environmental origin) were maintained at the microorganism’s bank of the Biomedical Science Institute (ICB-USP) of the São Paulo University, in São Paulo, Brazil. This isolates was submitted fungal strains determination, DNA extraction and purification, determination of genotype by URA5 gene RFLP of CGB-positive isolates, protease and phospholipase activity and susceptibility to antifungals. Results: Of six CGB positive isolates tested by RFLP-PCR, only four presented a genomic profile consistent C. gattii species (VGII), while two other were C. neoformans (VNI and VNIII), indicating the existence of canavanine-resistante C. neoformans isolates in the culture collections. The clinical isolates secreted higher levels of phospholipase and environmental isolates but no differences were observed for the protease levels. Almost all isolates were sensible to azoles and amphotericin B. Conclusion: We point out in this research the existence of C. neoformans strains resistant to canavanine and intrinsic characteristic of C. gatti. These results demonstrate the importance to perform a detailed characterization of isolates kept in culture collections.

关键词： Cryptococcus Complex Genotyping Protease Phospholipase Etest Culture Collection

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Development of Psychic Pain in the Context of Microcephaly and Abortion: Systematic Review and Meta-Analysis

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Health 2017年第2期9卷 376-391页

作者： Uilna Naté rcia Soares Feitosa Danilo Ferreira de Sousa Poliana Moreira de Medeiros Carvalho Raquel Guimarã es Kanda Joaquim Alves diniz Luis Clescivan Ferreira Nobre Dayse Christina Rodrigues Pereira Luz Wilma José Santana Cí cera Jaqueline Sobreira Andriola Né lio Barreto Vieira Antonio Souto Gouveia Alberto Olavo Advincula Reis Caio Parente Barbosa Modesto leite Rolim Neto Programa de Pó s-Graduaç ã o em Ciê ncias da Saú de Faculdade de Medicina do ABC-FMABC Santo Andr&eacute S&atilde o Paulo Brazil Faculdade de Juazeiro do Norte FJN Juazeiro do Norte Cear&aacute Brazil Faculdade de Medicina Est&aacute cio/FMJ Juazeiro do Norte Cear&aacute Brazil Faculdade de Medicina Universidade Federal do Cariri-UFCA Barbalha Cear&aacute Brazil Programa de Pó s-Graduaç ã o em Saú de Pú blica Universidade de S&atilde o Paulo-USP S&atilde o Paulo Brazil

Background: In the year of 2015, evidence of a relation between Zika virus and the development of microcephaly in pregnant women who acquired the infection started to come up. Thus, it is extremely necessary that the analysis of the affliction affects these women in a biopsychosocial context, in order to discuss and comprehend in a more reliable manner the affection from the perception of the disease, origin, and psychic pain mechanisms. Methods: Systematic review with meta-analysis, using the PRISMA protocol. The study period was 2016 and the keywords included “microcephaly” and “abortion”, one at a time and then combined with the Boolean operator “AND.” The statistical analysis was done using the BioEstat 5.0 program. Calculation was based on an adjustment of Mantel-Haenszel random effect. Results: 41,046 registrations were found. Of this total, 40,992 articles were excluded because they only mentioned the fact or referred to spontaneous abortion, did not analyze abortion on the eyes of microcephaly, only treated with Zika virus, or were repeated. Limitations: Psychic pain and suffering need studies in the mother/son/disease relation, which were not found in the databases. The article was based on other sources. Conclusions: The pains vary in different areas of the female life, since internal conflicts, gender violence, stress, fear, insecurity, psychologic torture, grieve, loneliness, among other kinds of psychic suffering. More studies on psycho-emotional themes need to be promoted to discuss the female suffering on a wider vision, closer to the reality faced by these women.

关键词： Abortion Microcephaly Psychic Pain

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