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Qualitative and quantitative detection using eDNA technology: A case study of Fenneropenaeus chinensis in the Bohai Sea

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Aquaculture and Fisheries 2020年第3期5卷 148-155页

作者： Miao Lia Xiujuan Shan Weiji Wang xiaosong ding Fangqun Dai ding Lv Huanhuan Wu Key Laboratory of Sustainable Development of Ma Fisheries Ministry of Agriculture and Rural AffairsShandong Provincial Key Laboratory of Fishery Resources and Ecological EnvironmentYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266237China

Accurate knowledge of species distributions and population dynamics is the basis for conservation ***, for certain species with unique life histories and very small populations, monitoring species distributions and population dynamics is extremely difficult. Recently, newly developed eDNA technology has been widely used in species monitoring, biodiversity assessments, and biomass assessments. In this research, we studied Fenneropenaeus chinensis in the Bohai Sea. We used a 0.45 μm glass fiber filter combined with a vacuum to filter 2 L seawater samples for eDNA enrichment from 54 stations in June and 60 stations in August. A DNeasy Blood and Tissue kit was used to extract the eDNA. After DNA extraction, the specific primers and probes of the mitochondrial DNA COI gene of F. chinensis were designed. Real-time quantitative PCR was used to qualitatively and quantitatively analyze the eDNA in the Bohai Sea. The temporal and spatial distribution of F. chinensis was detected by the eDNA technique. The relationship between the eDNA copy number and trawl-netted F. chinensis biomass was explored. The applicability and sensitivity of eDNA technology in marine crustacean research were verified. The results showed that the mtDNA COI gene of F. chinensis was successfully amplified in the 54 water samples collected in June, and the detection rate reached 100%. Detections occurred in only 23 of the 60 stations sampled in August. F. chinensis had a detection rate of only 38%. The copy number of eDNA obtained by realtime PCR was fitted to the density of F. chinensis captured by bottom trawling. There was not a significant positive correlation between copy number and biomass. The results obtained through this research will provide a method and theoretical basis for the application of eDNA technology in marine crustacean research.

关键词： Bohai Sea eDNA Fenneropenaeus chinensis Spatial and temporal distribution Biomass

Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo

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Progress in Natural Science:Materials International 2007年第9期17卷 1029-1034页

作者： Chen Xuemei,Yang Yumin,Wu Jian,Zhao Yahong,ding Fei and Gu xiaosong(Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China) Jiangsu Key Laboratory of Neuroregeneration Nantong University Nantong 226001 China

Silk fibroin(SF)has been used extensively in the biomedical field including tissue engineering for the generation of artificial bones,skins or *** have previously reported on good in vitro biocompatibility of SF fibers with peripheral nerve tissues and *** the present study,we developed a novel design of the SF-based artificial nerve graft(SF graft)which was composed of a SF-nerve guidance conduit(NGC)inserted with SF *** assay was performed to determine the cytotoxicity of the SF-NGC extract fluid on the cultured L929 cells derived from an immortalized mouse fibroblast cell *** addition,this SF graft was implanted into adult rats for bridging a 10-mm long sciatic nerve *** following-up experiments at initial stage(1-4 week)of nerve regeneration including routine blood tests and histochemical investigation were conducted to evaluate the in vivo biocompatibility of the SF graft with peripheral *** results demonstrated that the SF-NGC graft was biocompatible with the surrounding tissues and cells due to its low inflammatory potential with a grade 0 under the *** guidelines and it was generally suitable to a certain degree for bridging peripheral nerve defects in virtue of supporting Schwann cell adherence,expansion and *** the SF graft is a promising alternative to classical autografts for peripheral nerve repair.

关键词： silk fibroin artificial nerve graft nerve guidance conduit biocompatibility cytotoxicity

The Multi-period Immediate Delivery Problem with a Comprehensive Indicator for Order Selection

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The Multi-period Immediate Delivery Problem with a Comprehen...

Dynamics of shoreline and land reclamation from 1985 to 2015 in the Bohai Sea, China

2021智慧城市与智能建造高端论坛

作者： xiaosong ding Hu Liu Xi Chen International Business School Beijing Foreign Studies University DECIDE Group Department of Computer and Systems SciencesStockholm University

The immediate delivery problem is a variant of vehicle routing problem with stochastic demand,in which not all customers are known in advance,but are revealed while the system *** this paper,we investigate a multi-period immediate delivery problem with a comprehensive indicator for order *** particular,we model the order selection as a sequential decision-making *** the immediacy of delivery tasks,the mismatch between demand and capacity,and the autonomy of the delivery staff,we propose herein a new comprehensive indicator consisting of distance,time pressure,and order revenue as the order selection criterion to help drivers choose the right *** the nearest neighbor algorithm is adopted to optimize routes ***,extensive simulation experiments show that the proposed indicator performs stable and better than the other single indicators.

关键词： Vehicle Routing Problem Dynamic Requests Multi Period Order Selection Nearest Neighbor Algorithm

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Journal of Geographical Sciences 2019年第12期29卷 2031-2046页

作者： ding xiaosong SHAN Xiujuan CHEN Yunlong JIN Xianshi MUHAMMED Forruq Rahman College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China Laboratory for Marine Fisheries Scienee and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237ShandongChina Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsShandong Provincial Key Laboratory of Fishery Resources and Eco-environmentYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071ShandongChina College of Fisheries Ocean University of ChinaQingdao 266003ShandongChina

Extraction and analysis of the shoreline and land reclamation patterns are important for studies on topics such as the dynamics of coastal wetland ecological environments,transportation and exchange of material energy in coastal regions,and recruitment of fishery ***-temporal variations in the shoreline and land reclamation in the Bohai Sea were analyzed based on 49 Landsat images of 7 periods from 1985 to *** following conclusions were drawn.(1)The extracted shoreline data based on visual interpretation had high precision,and the shoreline extraction errors could be controlled within the theoretical range.(2)Over the past 30 years,the shoreline of the Bohai Sea has exhibited an average rate of change of 188.47 m/a and an average accretion distance of 3.55×10^3 m toward the *** fastest rate of shoreline change occurred in Laizhou Bay(134.78 m/a),followed by Bohai Bay(128.20 m/a)and Liaodong Bay(61.69 m/a).(3)The average rate of reclamation was 3.25×10^4 ha/a in the Bohai Sea,where the total area of aquaculture land,unused land,and salt land exceeded 60%of the total reclamation area.(4)The geometric shape of the bay became increasingly complicated from year to year,and the geometric center of gravity of the bay moved rapidly toward the *** addition,the area of the bay showed a significant decreasing ***,to protect the function and structure of the ecosystem in coastal regions,we must control the scale and rate of land reclamation in the future.

关键词： shoreline change land reclamation shape index of the bay Bohai Sea China

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急性心肌梗死合并高血糖的诊治

中国糖尿病杂志 2019年第12期27卷 956-958页

作者：丁晓松李虹伟首都医科大学附属北京友谊医院心内科

急性心肌梗死(AMI)是严重威胁患者生命的疾病,近半数AMI患者合并高血糖,预后较差。本文对AMI合并高血糖的机制、不良预后及诊治进行论述。

关键词：高血糖急性心肌梗死

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基于GIOWHA算子的汇率组合预测模型

统计与决策 2018年第12期34卷 9-13页

作者：丁小松杨桂元安徽财经大学统计与应用数学学院

文章采用三种模型：自回归模型、ETS指数平滑状态空间模型和非参数方法中的局部多项式回归模型对人民币汇率进行点预测,在此基础上引入广义诱导有序加权调和平均算子（GIOWHA）的概念,以预测值和实际值的p次幂倒数误差的平方和最小为准... 详细信息

文章采用三种模型：自回归模型、ETS指数平滑状态空间模型和非参数方法中的局部多项式回归模型对人民币汇率进行点预测,在此基础上引入广义诱导有序加权调和平均算子（GIOWHA）的概念,以预测值和实际值的p次幂倒数误差的平方和最小为准则,构建基于GIOWHA算子的最优变权系数组合预测模型。结果表明GIOWHA组合预测模型具有更精准的预测能力。

关键词：自回归 ETS指数平滑局部多项式回归 GIOWHA算子组合预测

基于SVAR模型的我国货币政策效应的实证分析

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鸡西大学学报（综合版） 2016年第12期16卷 75-80页

作者：丁小松安徽财经大学统计与应用数学学院安徽蚌埠233030

为研究我国货币政策货币渠道和信贷渠道传递的有效性,考察货币政策与消费、投资和产出之间的相互影响关系,基于VAR模型设置合理的约束条件,运用结构向量自回归模型(SVAR),对工业总产值、货币供应量、居民消费价格指数、金融机构贷款余... 详细信息

为研究我国货币政策货币渠道和信贷渠道传递的有效性,考察货币政策与消费、投资和产出之间的相互影响关系,基于VAR模型设置合理的约束条件,运用结构向量自回归模型(SVAR),对工业总产值、货币供应量、居民消费价格指数、金融机构贷款余额、社会零售商品总额、固定资产投资完成额、一年期贷款利率共七个变量之间的因果关系、脉冲响应以及方差分解进行分析。研究结果表明:我国货币政策只存在短期效应,货币供应量存在内生性;货币渠道的产出效应大于信贷渠道,信贷渠道的价格效应大于货币渠道;因此,在制定货币政策时应注重货币渠道和信贷渠道作用的相互补充。

关键词：货币渠道信贷渠道 SVAR模型有效性

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能源回购补偿机制下的联合生产与定价策略

系统科学与数学 2011年第10期31卷 1297-1305页

作者：张继红丁晓松陈曦北京外国语大学国际商学院北京100089

世界经济的快速发展和工业化进程的推进促使各国电力需求激增,电力供需矛盾为能源回购项目的发展提供了条件.考虑能源回购补偿机制下企业生产定价联合决策问题,建立了相应的具有线性需求函数的企业生产定价联合决策模型,研究了引入启动... 详细信息

世界经济的快速发展和工业化进程的推进促使各国电力需求激增,电力供需矛盾为能源回购项目的发展提供了条件.考虑能源回购补偿机制下企业生产定价联合决策问题,建立了相应的具有线性需求函数的企业生产定价联合决策模型,研究了引入启动成本和参与能源回购项目得到的经济补偿对企业生产与定价策略性质的影响,并利用k凸函数的概念证明了在线性需求函数的假设下,(s,S,P*)策略为最优生产定价策略.

关键词：能源回购经济补偿启动成本动态规划 (s,S,P~*)策略

基于林业生态环境保护下的国有林场经济发展创新分析

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环境与发展 2018年第10期30卷 210-210,212页

作者：丁晓松塞罕坝机械林场总场河北承德068453

本文针对塞罕坝机械林场在经营中存在的问题进行分析,并结合林业生态环境保护理论,在经营理念以及建设措施等方面提出了创新建议。

关键词：林业生态塞罕坝机械林场国有林场经济发展创新措施