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番杏LIM基因家族鉴定及TtWLIM1b的克隆与功能分析

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西南农业学报 2024年第5期37卷 944-954页

作者：刘浩丁倩倩王峥峰简曙光张美中国科学院海岛与海岸带生态修复工程实验室/广东省应用植物学重点实验室/中国科学院华南植物园广州510650 中国科学院大学北京100049

【目的】分析番杏[Tetragonia tetragonoides(Pall.) Kuntze]中TtLIM基因家族的序列组成、RNA-seq基因表达和异源表达生物学功能,探究其可能参与调控番杏植株发育和胁迫应答反应的分子机理,为发掘番杏抗逆功能基因及其在作物育种中的应... 详细信息

【目的】分析番杏[Tetragonia tetragonoides(Pall.) Kuntze]中TtLIM基因家族的序列组成、RNA-seq基因表达和异源表达生物学功能,探究其可能参与调控番杏植株发育和胁迫应答反应的分子机理,为发掘番杏抗逆功能基因及其在作物育种中的应用提供理论基础。【方法】通过前期对番杏[Tetragonia tetragonoides(Pall.) Kuntze]cDNA文库的筛选,获得一个编码番杏LIM基因的全长cDNA,命名为TtWLIM1b,对TtWLIM1b在酵母中进行功能分析。结合番杏全基因组测序信息,从番杏基因组中鉴定出16个TtLIM基因,对这些家族成员的染色体定位、所编码蛋白的理化性质、系统进化以及蛋白的保守模式进行综合的生物信息学分析;构建番杏与拟南芥、水稻和紫花苜蓿的LIM蛋白家族成员的进化树。最后,通过RNA-seq分析TtLIM基因家族成员在番杏不同组织以及在不同胁迫处理后的相对表达量。【结果】异源表达TtWLIM1b能够提高转基因酵母的重金属耐受性及耐热能力;番杏TtLIM家族蛋白成员均含有LIM结构域,而该结构域富含半胱氨酸。因此,TtLIMs也是富含半胱氨酸蛋白(Cysteine rich protein, CRP)的一种。序列分析表明,番杏LIM蛋白成员具有高度的保守性,且其成员的复制扩增较为明显;TtLIM基因家族成员在不同的番杏器官中广泛而有差异地表达。【结论】番杏TtLIMs可能参与调控番杏植株发育和胁迫应答反应。

关键词：番杏 LIM基因逆境胁迫金属耐受

Prescribed-Time Fully Distributed Nash Equilibrium Seeking Strategy in Networked Games

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IEEE/CAA Journal of Automatica Sinica 2024年第1期11卷 261-263页

作者： Cheng qian Lei ding the Institute of Advanced Technology for Carbon Neutrality College of Automation&the College of Artificial Intelligence Nanjing University of Posts and TelecommunicationsNanjing 210023China IEEE

Dear Editor, This letter is concerned with prescribed-time fully distributed Nash equilibrium seeking for networked games under directed graphs. An adaptive algorithm is proposed to ensure the convergence of all players to the Nash equilibrium without requiring any knowledge of global parameters. Moreover, it is theoretically proved that the convergence time of the proposed seeking strategy can be predefined based on practical requirements. Finally, a numerical example is presented to validate the effectiveness of the proposed method.

关键词： parameters seeking prescribed

Toward a rapid and convenient nanoplastic quantification method in laboratory-scale study based on fluorescence intensity

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Frontiers of Environmental Science & Engineering 2024年第5期18卷 117-126页

作者： Ruiqi Yan Sen Lin qian ding Lei Zhang Xia Yu Wentao Zhao qian Sui State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process School of Resources and Environmental EngineeringEast China University of Science and TechnologyShanghai200237China State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai200092China Shanghai Institute of Pollution Control and Ecological Security Shanghai200092China

The thorough investigation of nanoplastics(NPs)in aqueous environments requires efficient and expeditious quantitative analytical methods that are sensitive to environmentally relevant NP concentrations and convenient to *** analysis-based quantitative methods have been acknowledged as effective and rapid approaches for quantifying NP concentrations in laboratory-scale ***,we compared three commonly used optical response indicators,namely fluorescence intensity(FI),ultraviolet absorbance,and turbidity,to assess their performance in quantifying ***,orthogonal experiments were conducted to evaluate the influence of various water quality parameters on the preferred indicator-based quantification *** results revealed that FI exhibits the highest correlation coefficient(>0.99)with NP ***,the limit of quantification(LOQ)for various types of NPs is exceptionally low,ranging from 0.0089 to 0.0584 mg/L in ultrapure water,well below environmentally relevant *** variations in water quality parameters such as pH,salinity,suspended solids(SS),and humic acid,a robust relationship between detectable FI and NP concentration was ***,an increased matrix,especially SS in water samples,results in an enhanced LOQ for ***,the quantitative method remains applicable in real water bodies,especially in drinking water,with NP LOQ as low as 0.0157–0.0711 mg/*** exceeds the previously reported detectable concentration for 100 nm NPs at 40µg/mL using surface-enhanced Raman *** study confirms the potential of FI as a reliable indicator for the rapid quantification of NPs in aqueous environments,offering substantial advantages in terms of both convenience and cost-effectiveness.

关键词： Nanoplastic quantification Fluorescence intensity Drinking water Limit of quantification(LOQ) Matrix effects

Proteomic identification of proteins in exosomes of patients with atherosclerosis

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中国病理生理杂志 2016年第8期32卷 1525-1526页

作者： JIANG Mei QUAN Jing ZHANG Heng ding qian-qian XIANG Meng MENG Dan SUN Ning CHEN Si-feng Department of Neurology Shanghai Pudong New Area Gongli Hospital Department of Physiology and Pathophysiology Fudan University Shanghai Medical College

AIM: Atherosclerosis primarily involved systemic arteries. Luminal surface,a monolayer of endothelial cells,of artery directly exposes to blood and is susceptible to active substances in the blood. Exosomes contain significantly amount of proteins and RNAs. Exosomes can be good and bad for cells,depending on their component. Thus,exosomes may contribute to atherosclerosis by affecting endothelial cells. This study analyzed the relationship of exosome proteins and atherosclerosis. METHODS: Fifty-six patients and healthy subjects were recruited and divided into two comparisons: healthy subjects vs atherosclerosis( HS vs AS),and hypertension vs hypertension plus atherosclerosis( HT vs HT + AS). Serum exosomes were decoded by protein mass spectrometry. The protein profile and function were analyzed by gene ontology( GO). RESULTS: It was found that five child terms repeatedly appeared in"response to stimulus"and "immune system process"of BP of the two categories( HS vs AS and AS vs HT + AS): "positive regulation of innate immune response","immune response-activating signal transduction","activation of innate immune response","innate immune response-activating signal transduction"and "innate immune response activating cell surface receptor signaling pathway". Two child terms repeatedly showed in "binding"of MF of the two categories: "antigen binding"and "enzyme binding". Two proteins,PSMA6 and PSMA7,were repeatedly shown in the two categories. CONCLUSION: GO analysis was utilized for structure hierarchy"tree"to illustrate these proteins involved in various terms in BP,CC and MF. The PPI analysis supplied proteins which may play potentially important roles in AS process. Innate immune system and blood coagulation pathway contribute to AS formation. The proteins,PSMA6,PSMA7 and Annexin A2,may can be the new target proteins for prevention and treatment of AS.

关键词： PSMA

Increased N6-methyladenosine is related to the promotion of the methyltransferase METTL14 in ovarian aging

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Genes & Diseases 2024年第3期11卷 151-154页

作者： Chunfeng qian Zhenxing Liu Yonghong qian Jie ding Rulei Chen Han Su Minghong Shen Hong Li Boxian Huang State Key Laboratory of Reproductive Medicine and Offspring Health Suzhou Affliated Hospital of Nanjing Medicine and Offspring HealthSuzhou Affiliated Hospital of Nanjing Medical UniversitySuzhouMunicipal HospitalGusu SchoolNanjing Medical UniversitySuzhouJiangsu 215002China Department of Gynecology Affliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu School of Nanjing Medical UniversitySuzhouJiangsu 215002China Department of Pathology Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou Municipal HospitalGusu School of Nanjing Medical UniversitySuzhouJiangsu 215002China

The ovary is a vital female reproductive organ that functions to produce oocyte gametes and cyclically expressed sex hormones to maintain reproductive capacity and hormone *** aging,characterized by declines in follicle quantity and oocyte quality,leads to premature ovarian failure,premature ovarian insufficiency,or diminished ovarian reserve,thus contributing to female *** gene-expression signatures from RNA transcriptional level and post-transcriptional regulation are relative to oocyte quality and reproductive age.

关键词： promotion signature maintain

Preparation of wood-based hydrogel membranes for efficient purification of complex wastewater using a reconstitution strategy

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Frontiers of Environmental Science & Engineering 2024年第7期18卷 59-72页

作者： qian He Junkai Gao Zhongzhi Chen Yuanjing ding Mengsheng Xia Pengtao Xu Yan Chen School of Naval Architecture and Maritime Zhejiang Ocean UniversityZhoushan 316022China InnoTech Alberta P.O.Box 4000Hwy 16A&75 StreetVegrevilleAB T9C 1T4Canada

To avoid resource wastage and secondary environmental pollution,recycling and reusing waste wood powder is still a great ***,the poor viscosity and irregular pore size of wood powder limit its practical *** study,employed a green and convenient wood powder reconstitution strategy to achieve highly adhesive bonding and pore size control between wood powder particles,thus preparing a high-strength and super hydrophilic wood powder *** wood powder fibers were partially dissolved and regenerated to create a reconstituted wood powder hydrogel membrane,using waste wood powder as the raw *** wood powder reconstitution strategy offers advantages such as environmental friendliness,simplicity,cost-effectiveness,and strong ***,the materials exhibit excellent self-cleaning properties and *** by gravity,the membrane can purify oily wastewater and ***,the reconstitution strategy offers a new pathway for recycling wood powder.

关键词： Wood powder Reconstitution strategy Hydrogel membrane Recycling Wastewater

Protective effects of human induced pluripotent stem cell-derived exosomes on high glucose-induced injury in human endothelial cells

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中国病理生理杂志 2016年第8期32卷 1530-1531页

作者： ding qian-qian SUN Rui-ting WANG Ping-ping ZHANG Heng XIANG Meng MENG Dan SUN Ning CHEN Feng-yuan CHEN Si-feng Shanghai Medical College Fudan University

AIM: To investigate the effects of human induced pluripotent stem cell-derived exosomes( hi PSC-exo) on cell viability,capillarylike structure formation,and senescence in endothelial cells exposed to high glucose. METHODS: Exosomes were isolated from the conditional medium of hi PSCs and confirmed by transmission electron microscopy,nanoparticle tracking analysis,and Western blot analysis using Alix and CD63 as markers. hi PSC-exo were labeled with PKH26 for tracking. Cultured HUVECs were treated with high glucose(33 mmol / L) with or without hi PSC-exo(20 mg / L) for 48 h,and cell viability,capillary tube formation,and senescence were assessed. RESULTS: hi PSC-exo showed a typical cup shape and could be taken up by human umbilical vascular endothelial cells(HUVECs) in a concentration-dependent manner. When exposed to high glucose,viability and tube formation in HUVECs was significantly reduced,whereas the proportion of senescent cells was higher compared to that in control HUVECs( P ding pathological vascular remodeling that occurs in many diseases.

关键词： cell high stem

Dynamic Response of A Group of Cylindrical Storage Tanks with Baffles Considering the Effect of Soil Foundation

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China Ocean Engineering 2024年第1期38卷 129-143页

作者： SUN Ying WANG Jia-dong HUO Rui-li ZHOU ding GU Zhen-yuan qian Wang-ping School of Transportation and Civil Engineering Nantong UniversityNantong 226019China Faculty of Civil Engineering and Mechanics Jiangsu UniversityZhenjiang 212013China College of Civil Engineering Nanjing Tech UniversityNanjing 211816China

The sloshing in a group of rigid cylindrical tanks with baffles and on soil foundation under horizontal excitation is studied *** solutions for the velocity potential are derived out by the liquid subdomain *** models with mass-spring oscillators are established to replace continuous *** with the least square technique,Chebyshev polynomials are employed to fit horizontal,rocking and horizontal-rocking coupling impedances of soil,respectively.A lumped parameter model for impedance is presented to describe the effects of soil on tank structures.A mechanical model for the soil-foundation-tank-liquid-baffle system with small amount of calculation and high accuracy is proposed using the substructure *** analytical solutions are in comparison with data from reported literature and numerical codes to validate the effectiveness and correctness of the *** dynamic properties and seismic responses of the soil-tank system are given for the baffle number,size and location as well as soil parameter.

关键词： cylindrical tanks multiple annular baffles equivalent analytical model soil−structure interaction subdomain method dynamic response

Construction of ZnO@CDs@Co_(3)O_(4) sandwich heterostructure with multi-interfacial electron-transfer toward enhanced photocatalytic CO_(2) reduction

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Chinese Chemical Letters 2024年第4期35卷 147-152页

作者： Xiuzheng Deng Yi Ke Jiawen ding Yingtang Zhou Hui Huang qian Liang Zhenhui Kang Advanced Catalysis and Green Manufacturing Collaborative Innovation Center CNPC-CZU Innovation AllianceSchool of Petrochemical EngineeringChangzhou UniversityChangzhou 213164China Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control National Engineering Research Center for Marine AquacultureMarine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316004China

Photocatalytic conversion of CO_(2) into small-molecule chemical feedstocks can meet the growing demand for energy and alleviate the global warming. Herein, a p-n ZnO@CDs@Co_(3)O_(4) heterojunction with sandwich structure was constructed by calcination method of self-assembled ZIF-8@CDs@ZIF-67. The ZnO@CDs@Co_(3)O_(4) with well-defined interfacial structure exhibited the significantly enhanced photocatalytic CO_(2) reduction activity, and the optimal catalyst indicated the(CO + CH_(4)) evolution rate of 214.53μmol g^(-1)h^(-1) under simulated solar light, which was superior to ZnO, Co_(3)O_(4) and binary ZnO@Co_(3)O_(4).The internal cavity, exposed active sites, multiple interfaces and constructed p-n heterojunction can facilitate the light harvesting and photoexcited electron transfer. Besides, after introduction of CDs placed in the middle layer between ZnO and Co_(3)O_(4), CDs with excellent photoelectric property further promoted charge separation and migration. This work represents an appealing strategy to construct well-defined photocatalysts for boosting CO_(2) photoreduction.

关键词： Metal-organic framework Photocatalytic CO_(2) reduction Carbon dots p-n heterojunction Sandwich heterostructure