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Science China Chemistry 2012年第1期55卷 118-124页

作者： diao shu ZHAO Hong WANG WeiMao WU Chi Department of Chemistry The Chinese University of Hong Kong Hong Kong China The Hefei National Laboratory of Physical Science at Microscale Department of Chemical physics The University of Science and Technology of China Hefei 230022 China

Our dynamic laser light scattering(LLS) study shows that the current widely used protocols of dissolving amyloidogenic protein/peptide do not really result in a true solution;namely,there always exist a trace amount of interchain aggregates,which greatly affect the association kinetics,partially explaining why different kinetics were reported even for a solution with identical protein and ***,using a combination of the conventional dissolution procedure and our newly developed ultra-filtration method,we have developed a novel protocol to prepare a true solution of amyloidogenic protein/peptide without any interchain *** resultant solutions remain in their monomeric state for at least one week,which is vitally important for further study of the very initial stage of the interchain association under the physiological conditions because more and more evidence suggests that it is those small oligomers rather than large fabric aggregates that are *** addition,this study shows that combining static and dynamic LLS can lead to more physical and microscopic information about the protein association instead of only the size distribution.

关键词： amyloidogenic protein/peptide laser light scattering monomer ultra-filtration

Preparation of true solutions of monomeric amyloidogenic protein/peptide:A critical prerequisite for aggregation kinetic study

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中国科学：化学 2012年第2期42卷 229-230页

作者： diao shu ZHAO Hong WANG WeiMao WU Chi Department of Chemistry The Chinese University of Hong Kong Hong Kong China The Hefei National Laboratory of Physical Science at Microscale Department of Chemical Physics The University of Science and Technology ofChina Hefei 230022 China

关键词：聚合动力学淀粉样蛋白多肽单体关联动力学动态激光光散射正解制备

No consistent daily variation in DNA methylation detected in Populus nigra leaves by methylation-sensitive amplification polymorphism analysis

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Journal of Forestry Research 2017年第4期28卷 653-660页

作者： shu diao Yanbo Wang Changjun Ding Yingying Chang Lixiong Liang Yanan Gao Bingyu Zhang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China

DNA methylation, an epigenetic mechanism used by cells to control gene expression, has an important biological role in plant development and environmental fitness. Since plant DNA methylation is closely related to environmental conditions, variation during the day is expected. Here, in genetically identical plants of Populus nigra clone N46, DNA methylation changes in leaves over a 24 h period were detected using the methylation-sensitive amplification polymorphism method. The results showed different DNA methylation patterns in mature poplar leaves: not only in individuals at the same time, but also in samples at each of the six time during the day. In addition, night samples had a higher percentage of methylation than in morning samples. However, no statistically significant differences were found among the samples gathered at different times. Similar results were obtained for three other P. nigra clones with different genetic backgrounds. Real time qPCR showed that the DNA methyltransferase genes Pt-MET1 and Pt-SOM1 involved in CG DNA methylation in poplar were stable over a 24 h period in leaves of P. nigra N46 compared with circadian-controlled genes. That could be part of the reason that methylation of CCGG sites is stable in those leaves. That DNA methylation differed even in genetically identical plants indicates the specificity of DNA methylation changes in their genomes. No statistically significant differences in methylation changes were found between day and night, suggesting that DNA methylation is more stable than expected and is unlikely to be involved in circadian regulation in plants.

关键词： Cytosine methylation Epigenetic Populus nigra Methylation-sensitive amplification polymorphism (MSAP) Variation

Exploring the genetic features and signatures of selection in South China indigenous pigs

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Journal of Integrative Agriculture 2021年第5期20卷 1359-1371页

作者： diao shu-qi XU Zhi-ting YE Shao-pan HUANG shu-wen TENG Jin-yan YUAN Xiao-long CHEN Zan-mou ZHANG Hao LI Jia-qi ZHANG Zhe Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding/National Engineering Research Center for Breeding Swine Industry/College of Animal Science South China Agricultural UniversityGuangzhou 510642P.R.China

To explore the genetic features and signatures of selection in indigenous pigs from South China and Duroc pigs,259 pigs from six populations were genotyped using single-nucleotide polymorphism(SNP)*** component analysis(PCA),effective population size(Ne),linkage disequilibrium(LD),and signatures of selection were explored and investigated among the six pig *** results showed the Ne of five South China indigenous pig populations has been decreasing rapidly since 100 generations *** LD between pairwise SNP distance at 100 kb ranged from 0.16 to 0.20 for the five indigenous pig populations,while it was 0.32 for the Duroc ***,the LD of all six pig populations showed the opposite order at long distances(>5 Mb).Furthermore,15 potential signatures of selection associated with meat quality and age at puberty were exclusively detected in South China indigenous pigs,while eight potential signatures of selection associated with growth traits were detected in Duroc *** work provides valuable insights for the utilization and conservation of South China indigenous pigs.

关键词： pigs population structure effective population size SNP

Application on drilling parameter monitor in drilling engineering monitoring

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Global Geology 2008年第2期11卷 83-87页

作者： shu diao Zhenbao LING Wenjing LIANG College of Instrumentation and Electrical Engineering Jilin University Changchun 130026 China Key Laboratory of Geoexploration and Instrumentation of Education Ministry Jilin University Changchun 130026 China

The drilling parameter monitor is an important tool in drilling engineering applied to monitoring drilling process,carrying out scientific analysis and *** on discussing the present development situation of the domestic and foreign drilling machine parameter monitors,the metering scheme for vehicle--loaded drilling parameter monitor was *** using detection system for MSP430 single--chip microcomputer(SCM) in combination with peripheral circuit such as sensors,the drilling--rig control system was obtained to detect,and for every parameter in real--time display in order to keep operating the drilling rig *** experiment shows that the drilling parameter monitor reaches design requirements and can be applied to drilling engineering monitoring,which has characters such as simple structure,high credibility and low cost.

关键词： drilling parameter monitor sensor MSP430 single-chip microcomputer

Identifying the complex genetic architecture of growth and fatness traits in a Duroc pig population

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Journal of Integrative Agriculture 2021年第6期20卷 1607-1614页

作者： HANG Zhe CHEN Zi-tao diao shu-qi YE Shao-pan WANG Jia-ying GAO Ning YUAN Xiao-long CHEN Zan-mou ZHANG Hao LI Jia-qi Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding/National Engineering Research Center for Breeding Swine Industry/College of Animal Science South China Agricultural UniversityGuangzhou 510642P.R.China

In modern pig breeding programs,growth and fatness are vital economic traits that significantly influence porcine *** identify underlying variants and candidate genes associated with growth and fatness traits,a total of 1067 genotyped Duroc pigs with de-regressed estimated breeding values(DEBV)records were analyzed in a genome wide association study(GWAS)by using a single marker regression *** total,28 potential single nucleotide polymorphisms(SNPs)were associated with these traits of ***,VPS4 B,PHLPP1,and some other genes were highlighted as functionally plausible candidate genes that compose the underlying genetic architecture of porcine growth and fatness *** findings contribute to a better understanding of the genetic architectures underlying swine growth and fatness traits that can be potentially used in pig breeding programs.

关键词： pig GWAS growth trait fatness trait candidate gene

Genome-wide detection of selective signatures in a Duroc pig population

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Journal of Integrative Agriculture 2018年第11期17卷 2528-2535页

作者： diao shu-qi LUO Yuan-yu MA Yun-long DENG Xi HE Ying-ting GAO Ning ZHANG Hao LI Jia-qi CHEN Zan-mou ZHANG Zhe Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding/National Engineering Research Centre for Breeding Swine Industry/College of Animal Science South China Agricultural University Guangzhou 510642 P.R.China Liupanshui Academy of Agricultural Sciences Liupanshui 553001 P.R. China Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction Ministry of Education/College of Animal Sciences and Technology Huazhong Agricultural University Wuhan 430070 P.R. China

The Duroc pig has high adaptability and feeding efficiency, making it one of the most popular pig breeds worldwide. Over long periods of natural and artificial selection, genetic footprints, i.e., selective signatures, were left in the genome. In this study, a Duroc pig population （n=715） was genotyped with the Porcine SNP60K Bead Chip and the GeneSeek Genomic Profiler （GGP） Porcine Chip. The relative extended haplotype homozygosity （REHH） method was used for selective signature detection in a subset of the population （n=368）, selected to represent a balanced family structure. In total, 154 significant core regions were detected as selective signatures （,P〈0.01）, some of which overlap with previously reported quantitative trait loci associated with several economically important traits, including average daily gain and backfat thickness. Genome annotation for these significant core regions revealed a variety of interesting candidate genes including GATA3, TAF3, ATP5C1, and FGFI. These genes were functionally related to anterior/posterior pattern specification, phosphatidylinositol 3-kinase signaling, embryonic skeletal system morphogenesis, and oxidation-reduction processes. This research provides knowledge for the study of selection mechanisms and breeding practices in Duroc and other pigs.

关键词： Duroc selective signature candidate genes REHH

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明前期明朝向朝鲜索征的“别贡”

东北师大学报（哲学社会科学版） 2009年第3期 60-66页

作者：刁书仁东北师范大学历史文化学院吉林长春130024

史学界通常认为,在中朝两国关系发展史上,明朝与李氏朝鲜的宗藩关系最为典型,是两国关系最好的时期。两国之间保持着频繁的往来,朝鲜定期遣使朝贡,明朝对其贡献的方物,本着"厚往薄来"的原则,给予丰厚的回赐。而对明朝不时向朝鲜索征贡... 详细信息

史学界通常认为,在中朝两国关系发展史上,明朝与李氏朝鲜的宗藩关系最为典型,是两国关系最好的时期。两国之间保持着频繁的往来,朝鲜定期遣使朝贡,明朝对其贡献的方物,本着"厚往薄来"的原则,给予丰厚的回赐。而对明朝不时向朝鲜索征贡物的史实,往往给以忽视,或避而不谈。而明前期,明朝向朝鲜索征"处女"、"火者"、"海青鹰子"等就属于"别贡"性质。这种"别贡"给朝鲜带来沉重的负担,给两国关系带来很大的影响。

关键词：明朝索征朝鲜“别贡”

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“长吉图”地区与古代东亚各国的交往

东北师大学报（哲学社会科学版） 2010年第4期 60-66页

作者：刁书仁东北师范大学历史文化学院吉林长春130024

"长吉图"地处东北亚区域中心。这一地区历史悠久,先后有肃慎、沃沮、夫余、高句丽、靺鞨、女真、满族等民族在这里生息繁衍,并先后建立夫余、高句丽、渤海、辽、金、元、后金(清)等政权。这些民族和政权与陆地相连的朝鲜半岛的新罗、高... 详细信息

"长吉图"地处东北亚区域中心。这一地区历史悠久,先后有肃慎、沃沮、夫余、高句丽、靺鞨、女真、满族等民族在这里生息繁衍,并先后建立夫余、高句丽、渤海、辽、金、元、后金(清)等政权。这些民族和政权与陆地相连的朝鲜半岛的新罗、高丽、李氏朝鲜和隔海相望的日本有着频繁的友好交往。这种友好交往无疑对今天"长吉图"开发开放有所裨益。

关键词： “长吉图” 东亚历史交往

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明代女真与朝鲜的贸易

史学集刊 2007年第5期 72-78页

作者：刁书仁扬州大学社会发展学院江苏扬州225002

明代居住在图们江、鸭绿江流域的女真族农业比较落后,手工业不发达,在远离明辽东地区的情况下,所需生活必需品只有通过与临近的朝鲜贸易才能解决。因此,女真与朝鲜的贸易往来比较频繁。贸易形式通常是女真携带"土物"到朝鲜京城"进献",... 详细信息

明代居住在图们江、鸭绿江流域的女真族农业比较落后,手工业不发达,在远离明辽东地区的情况下,所需生活必需品只有通过与临近的朝鲜贸易才能解决。因此,女真与朝鲜的贸易往来比较频繁。贸易形式通常是女真携带"土物"到朝鲜京城"进献",从中得到朝鲜的"回赐"与边境互市贸易两种形式。这种贸易补充了各自所需,丰富了双方的经济生活,尤其是给女真社会的发展带来积极的影响。

关键词：女真朝鲜贸易