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Seasonal and Genetic Characteristics of Human Metapneumovirus Circulating—Henan Province,China,2017-2023

China CDC weekly 2024年第20期6卷 450-456,I0001页

作者： Zhibo Xie Zhen Zhu Jin Xu Naiying Mao Aili cui Wenhui Wang Yage Wang Zhibo Zhang Baicheng xia Haoran Wang Zhen Sun Yan Zhang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious diseases NHC Key Laboratory of Medical Virology and Viral DiseaseNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Henan Provincial Center for disease Control and Prevention Luohe CityHenan ProvinceChina Luohe Center for disease Control and Prevention Luohe CityHenan ProvinceChina Luohe Central Hospital Luohe CityHenan ProvinceChina

Introduction:This study examines the seasonal and genetic characteristics of human metapneumovirus(HMPV)in Henan from 2017 to ***:Samples from patients with acute respiratory infection(ARI)testing positive for HMPV were subjected to real-time reverse transcription polymerase chain reaction The G gene was amplified and sequenced from these samples for epidemiological and phylogenetic ***:We enrolled 2,707 ARI patients from October 2017 to March 2023,finding an HMPV positivity rate of 6.17%(167/2,707).Children under five exhibited the highest infection rate at 7.78%(138/1,774).The 2018 and 2019 HMPV outbreaks predominantly occurred in spring(March to May),with peak positivity rates of 31.11%in May 2018 and 19.57%in May 2019.A notable increase occurred in November 2020,when positivity reached a historic high of 42.11%,continuing until January *** February 2021 through March 2023,no significant seasonal peaks were observed,with rates ranging from 0%to 8.70%.Out of 81 G gene sequences analyzed,46.91%(38/81)were identified as subtype A(A2c:45.67%,37/81;A2b:1.23%,1/81)and 53.09%(43/81)as subtype B(B1:9.88%,8/81;B2:43.21%,35/81).Notably,an AAABBA switch pattern was observed in HMPV *** dominant strains were A2c111nt-dup in subtype A and B2 in subtype ***:Six years of surveillance in Henan Province has detailed the seasonal and genetic dynamics of HMPV,contributing valuable insights for the control and prevention of HMPV infections in *** findings support the development of targeted HMPV vaccines and immunization strategies.

关键词： prevention finding seasonal

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Washed microbiota transplantation for Crohn’s disease:A metagenomic,metatranscriptomic,and metabolomic-based study

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World Journal of Gastroenterology 2024年第11期30卷 1572-1587页

作者： Shi-Ju Chen Da-Ya Zhang xia Wu Fa-Ming Zhang Bo-Ta cui Yi-Hao Huang Zu-Lun Zhang Rui Wang Fei-Hu Bai Graduate School Hainan Medical UniversityHaikou 571199Hainan ProvinceChina Department of Microbiota Medicine&Medical Center for digestive diseases The Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of Gastroenterology The Second Affiliated Hospital of Hainan Medical UniversityHaikou 570216Hainan ProvinceChina

BACKGROUND Fecal microbiota transplantation(FMT)is a promising therapeutic approach for treating Crohn’s disease(CD).The new method of FMT,based on the automatic washing process,was named as washed microbiota transplantation(WMT).Most existing studies have focused on observing the clinical ***,the mechanism of action of FMT for the effective management of CD-particularly in-depth multi-omics analysis involving the metagenome,metatranscriptome,and metabolome-has not yet been *** To assess the efficacy of WMT for CD and explore alterations in the microbiome and metabolome in response to *** We conducted a prospective,open-label,single-center clinical *** CD patients underwent *** clinical responses(defined as a decrease in their CD Activity Index score of>100 points)and their microbiome(metagenome,metatranscriptome)and metabolome profiles were evaluated three months after the *** Seven of the 11 patients(63.6%)showed an optimal clinical response three months *** microbiome diversity significantly increased after WMT,consistent with improved clinical *** of the metagenome and metatranscriptome analyses revealed consistent alterations in certain strains,such as Faecalibac-terium prausnitzii,Roseburia intestinalis,and Escherichia *** addition,metabolomics analyses demonstrated that CD patients had elevated levels of various amino acids before treatment compared to the ***,levels of vital amino acids that may be associated with disease progression(e.g.,L-glutamic acid,gamma-glutamyl-leucine,and prolyl-glutamine)were reduced after *** WMT demonstrated therapeutic efficacy in CD treatment,likely due to the effective reconstruction of the patient’s ***-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

关键词： Crohn’s disease Clinical trials Fecal microbiota transplant Metagenome Metatranscriptome Metabolome

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SiRAP2-12,a Positive Regulatory Factor,Effectively Improves the Waterlogging Tolerance of Foxtail Millet(Setaria italica)

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 445-465页

作者： Xueyan xia xiaohong Fu Yu Zhao Jihan cui Nuoya xiao Jingxin Wang Yiwei Lu Meihong Huang Cheng Chu Jia Zhang Mengxin Yang Shunguo Li Jianfeng Liu Institute of Millet Crops Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-Construction byMinistry and Province)Ministry of Agriculture and Rural Affairs/The Key Research Laboratory of Minor Cereal Crops of Hebei ProvinceShijiazhuang050035China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding071002China

Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield *** family plays an important role to plant adversity *** our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging ***,a total of 28 ERF family members were obtained,which have a complete open read-ing *** studied the evolution and function of SiERF family and how they affected the waterlogging *** was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other *** them,the SiRAP2-12 expression was obviously signiﬁcantly up-regulated in foxtail millet after 5d water-logging *** SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased signiﬁ*** details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,*** contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,*** content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))***,we found that ethylene content was signiﬁcantly ***,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging *** together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress.

关键词： Foxtail millet waterlogging SiRAP2-12 VIGS ethylene

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Capsazepine诱导人肝癌HepG2细胞凋亡机制研究

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中华肿瘤防治杂志 2015年第10期22卷 764-769页

作者：韩璐狄翠霞毛爱红颜家玮张红中国科学院近代物理研究所甘肃兰州730000 中国科学院重离子束辐射生物医学重点实验室甘肃兰州730000 甘肃省重离子束辐射医学应用基础重点实验室甘肃兰州730000 中国科学院大学生命科学学院北京100049

目的研究瞬时感受器电位离子通道(transient receptor potential vani1loid 1,TRPVl)阻滞剂Capsazepine对人肝癌HepG2细胞凋亡的机制。方法采用MTT法检测浓度分别为0.01、0.1、1、25、100μmol/L Capsazepine作用于HepG2细胞24h后对细胞生长的影响;Hoechst 33258染色法观察0.1和10μmol/L Capsazepine作用于HepG2细胞24h后细胞的形态学改变;流式细胞术观察0.1和10μmol/L Capsazepine作用于HepG2细胞24h后细胞的凋亡率和周期的变化;实时荧光定量PCR技术(real time-PCR)、蛋白质印迹法和激光共聚焦法检测0.1和10μmol/L Capsazepine作用于HepG2细胞24h后,细胞中离子通道受体蛋白TRPV1,以及凋亡相关因子p53,Bcl-2和Bax在mRNA和蛋白水平的表达情况。结果随着药物浓度的增大,Capsazepine对体外培养的HepG2细胞有抑制增殖作用,且具有剂量相关性;0.01、0.1、1、25、100μmol/L的Capsazepine作用于HepG2细胞24h后,HepG2细胞存活率分别为84.77%、73.83%、70.58%、61.42%和48.48%,F=176.523,P<0.001。流式细胞术检测显示,Capsazepine可致HepG2细胞阻滞于G0/G1期,并诱导其凋亡;0.1与10μmol/L的Capsazepine作用于HepG2细胞24h,与对照组相比,G0/G1期细胞比例分别为(65.48±0.04)%和(72.14±0.30)%,F=187.791,P<0.001;对照组与Capsazepine处理组细胞凋亡率分别为(3.25±1.08)%和(21.50±4.85)%,t=0.000 26,P=0.026。Hoechst荧光染色法可见,随着药物浓度的增大,细胞的生长变慢,胞质变疏松,细胞核皱缩,出现典型的细胞凋亡形态。蛋白质印迹法检测可见,对照组与0.1和10μmol/L的Capsazepine作用于HepG2细胞24h,离子通道受体蛋白TRPV1的表达量分别为0.62±0.03、0.58±0.04和0.38±0.04,随着Capsazepine浓度的增加,TRPV1的表达量显著下降,差异有统计学意义,F=38.926,P<0.001。在对照组与实验组中,p53蛋白表达量分别为0.25±0.007、0.26±0.005和0.31±0.01,Bax蛋白表达量分别为1.49±0.16、2.87±0.06和3.12±0.15,Bcl-2蛋白表达量分别为0.70±0.08、0.35±0.02和0.37±0.04,随着Capsazepine浓度的增加,促进凋亡相关蛋白p53、Bax表达上调和Bcl-2表达下调,各实验组与对照组比较,差异均有统计学意义,F值分别为50.968、129.002和42.626,P值均<0.001。结论离子通道TRPV1阻滞剂Capsazepine可抑制肝癌HepG2细胞的增殖,促进G0/G1的周期阻滞,从而诱导其凋亡,其机制可能与上调凋亡相关因子p53和Bax蛋白表达,以及下调Bcl-2蛋白表达有关。

关键词：瞬时感受器电位离子通道 Capsazepine HepG2细胞细胞凋亡

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放射性药物在临床诊断和治疗中的应用进展

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中华肿瘤防治杂志 2022年第18期29卷 1354-1360页

作者：赵大鹏陈小花苏玮张桃桃张红狄翠霞中国科学院近代物理研究所中国科学院重离子束辐射生物医学重点实验室甘肃省重离子束辐射医学应用基础重点实验室甘肃兰州730000 中国科学院大学生命科学学院北京100049

目的探讨放射性药物在临床诊断和治疗中的应用,并总结最近研究进展。方法应用PubMed及中文期刊全文数据库(CNKI)检索系统,以“放射性药物、同位素药物、正电子发射型计算机断层显像、单光子发射计算机断层成像术、肿瘤靶向治疗”为中文... 详细信息

目的探讨放射性药物在临床诊断和治疗中的应用,并总结最近研究进展。方法应用PubMed及中文期刊全文数据库(CNKI)检索系统,以“放射性药物、同位素药物、正电子发射型计算机断层显像、单光子发射计算机断层成像术、肿瘤靶向治疗”为中文关键词,以"radiopharmaceuticals,isotope drugs、positron emission computed tomography、Single-photon emission computed tomography、tumor targeted therapy"为英文关键词,检索1990-01-01-2022-04-30的相关文献。纳入标准:放射性药物最新临床试验;放射性药物研发进展。排除标准:会议摘要、中文学位论文等非正式发表文献。最终共纳入文献49篇。结果放射性药物在临床诊断和治疗中已有着广泛应用,诊断用放射性药物发展十分迅速,与之配合的SPECT和PET技术已经投入应用,对于肿瘤等疾病的早期诊断和发现有着重要作用。在临床诊断中配合新型CT技术的放射性药物正在不断地研发,在临床治疗中α核素放射性药物等新型药物的出现也为癌症等疾病治疗提供了新思路。结论放射性药物的研发对于肿瘤等疾病诊断和治疗有着重要意义,在早期诊断、杀灭损伤细胞和临床医学成像等领域发挥不可忽视的作用。

关键词：放射性药物核医学断层扫描靶向治疗配体

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Transient diabetes mellitus with ABCC8 variant successfully treated with sulfonylurea:Two case reports and review of literature

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World Journal of diabetes 2024年第8期15卷 1811-1819页

作者： Ling-Hua Shen Yan cui Dong-xia Fu Wei Yang Sheng-Nan Wu Hui-Zhen Wang Hai-Hua Yang Yong-Xing Chen Hai-Yan Wei Department of Endocrinology and Metabolism Henan Key Laboratory of Children's Genetics and Metabolic DiseasesChildren's Hospital Affiliated to Zhengzhou UniversityHenan Children's Hospital Zhengzhou Children's HospitalZhengzhou 450018Henan ProvinceChina

BACKGROUND Transient neonatal diabetes mellitus(TNDM)is a rare form of diabetes mellitus that usually presents within the first 6 mo of *** often enter remission within several months,although relapse can occur later in *** in the ABCC8 gene,which encodes the sulfonylurea receptor 1 of the ATP-sensitive potassium channel in pancreatic beta cells,are associated with TNDM and permanent neonatal *** study describes a novel de novo c.3880C>T heterozygous ABCC8 variant that causes TNDM and can be treated with sulfonylurea *** SUMMARY We retrospectively analyzed 2 Chinese patients with TNDM who were diagnosed,treated,or referred for follow-up between September 2017 and September *** patients were tested for mutations using targeted next-generation *** with neonatal diabetes mellitus caused by a c.3880C>T heterozygous missense variant in the ABCC8 gene have not been reported *** children had an onset of post-infectious diabetic ketoacidosis,which is worth *** a follow-up visit after discontinuing insulin injection,oral glyburide was found to be effective with no adverse *** Early genetic testing of neonatal diabetes mellitus aids in accurate diagnosis and treatment and helps avoid daily insulin injections that may cause pain.

关键词： Neonatal diabetes mellitus ABCC8 Sulfonylurea receptors 1 KATP channels Sulfonylurea Glyburide Insulinulin Case report

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农地整治对农户收入差距的影响研究

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农业技术经济 2024年第8期 20-34页

作者：崔民夏显力西北农林科技大学经济管理学院杨凌712100

本文利用山东、河南两省的830份农户调研数据,采用内生转换模型,在考虑不可观测因素导致的选择性偏误基础上,实证分析农地整治对农户收入水平的影响,并运用Fields分解法检验农地整治对农户收入差距的作用方向及程度。结果表明:第一,基... 详细信息

本文利用山东、河南两省的830份农户调研数据,采用内生转换模型,在考虑不可观测因素导致的选择性偏误基础上,实证分析农地整治对农户收入水平的影响,并运用Fields分解法检验农地整治对农户收入差距的作用方向及程度。结果表明:第一,基于反事实假设,农户若未参与农地整治,其家庭总收入将下降13.1%,即农地整治显著提高了农户的家庭总收入。从收入结构上来看,农地整治对农业收入的促增作用大于非农收入;第二,农地整治扩大农户之间的收入差距;贡献率为26.4%,农地整治不仅没有实现矫正农业收入差距的初衷,反而产生了“逆向分配”的结果;第三,低收入农户参与农地整治程度不足和高收入农户在农地整治中获得更高的收益率,是农地整治扩大农户收入差距的重要原因;第四,农地整治引发了不同收入水平农户社会公平感的分化。因此,在考虑如何通过农地整治增加农户收入时,有必要将问题的焦点从效率延伸到公平,重点关注农地整治中处于相对不利地位的低收入农户的收入增长问题。

关键词：农地整治收入水平收入差距内生转换回归模型(ESR) Fields分解

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AI大语言模型背景下的修辞学研究

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当代修辞学 2024年第3期 24-36页

作者：崔希亮赵霞北京语言大学国际中文教育研究院北京100083 剑桥大学亚洲与中东学院英国剑桥

本文旨在探讨人工智能背景下当代修辞学的发展及其面临的挑战。文章首先简要介绍大语言模型发展的过程,回顾西方修辞学和中国修辞学的传统理论框架,指出在大语言模型背景下当代修辞学研究的重新定位,内容包括:修辞学的本体研究、学科融... 详细信息

本文旨在探讨人工智能背景下当代修辞学的发展及其面临的挑战。文章首先简要介绍大语言模型发展的过程,回顾西方修辞学和中国修辞学的传统理论框架,指出在大语言模型背景下当代修辞学研究的重新定位,内容包括:修辞学的本体研究、学科融合、情感转向、数字化、认知研究、生态转向。然后从语言理解和生成的角度对五家大语言模型进行了测试。文章发现大语言模型已经具备了一般的语言修辞能力,但是在理解复杂语义关系方面还存在一定的局限性。大语言模型的修辞理解和修辞生成能力给我们的启发是人工智能为修辞学带来了新的研究路径和变革的可能性。最后,文章展望了人工智能与修辞学未来的发展趋势,指出了人工智能大语言模型对修辞学研究可能带来的影响。

关键词：人工智能大语言模型修辞学语言理解与生成

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百合组织培养和遗传转化的研究进展

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西北植物学报 2006年第4期26卷 858-863页

作者：狄翠霞张满效谢忠奎陈拓安黎哲兰州大学干旱与草地农业生态教育部重点实验室兰州730000

百合是单子叶球茎类观赏花卉,具有食用和药用的作用。本文综述了国内外百合组织培养和遗传转化研究进展。包括营养器官、生殖器官和原生质体再生系统的建立。详细介绍了基因转化的方法,例如农杆菌、基因枪、电激穿孔等。浅析百合外源基... 详细信息

百合是单子叶球茎类观赏花卉,具有食用和药用的作用。本文综述了国内外百合组织培养和遗传转化研究进展。包括营养器官、生殖器官和原生质体再生系统的建立。详细介绍了基因转化的方法,例如农杆菌、基因枪、电激穿孔等。浅析百合外源基因的表达,例如GUS、半夏凝集素基因、几丁质酶基因等。同时,讨论了百合遗传转化中存在的问题,以期为百合基因工程方面的研究提供依据。

关键词：百合组织培养遗传转化

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Evaluation of the famous classic formula Sanhua decoction based on network pharmacology and multi-component quantitative analysis

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Traditional Medicine Research 2024年第1期9卷 1-13页

作者： Xin Zhang Wan-cui Wang Jin-Kui Zhang Wei-Mei Zhang Peng-Wang Wang Peng-Cheng Lin Yong-Chang Lu xia Li Wen-Yuan Gao College of Pharmacy Qinghai Minzu UniversityXining 810000China Qinghai Key Laboratory of Phytochemistry on Qinghai-Tibet Plateau Qinghai Minzu UniversityXining 810000China School of Pharmaceutical Science and Technology Tianjin UniversityTianjin 300000China Qinghai Engineering Technology Research Center of Modern Tibetan Medicine Creation Qinghai Minzu UniversityXining 810000China

Background:Sanhua decoction has significant effects in the treatment of *** study of the Sanhua decoction material benchmark was carried out to analyze the value transfer relationship between the Chinese herbal pieces and the substance ***:Network pharmacology was employed to investigate the potential active components and molecular mechanisms of Sanhua decoction in the treatment of stroke.15 batches of Sanhua decoction lyophilized powder were prepared using traditional formulas and subjected to high-performance liquid chromatography analysis to generate fingerprints of the Sanhua decoction substance ***,a multi-component quantitative analysis method was established,allowing for the simultaneous determination of ten components,to study the transfer of quantity values between pieces and substance ***:60 active ingredients were screened from Sanhua decoction by network pharmacology,of which gallic acid,magnolol honokiol,physcion,and aloe-emodin may have a greater effect than other active components.63 key targets and 134 pathways were predicted as the potential mechanism of Sanhua decoction in treating *** fingerprint similarity of the Sanhua decoction substance benchmarks was found to be good among the 15 batches,confirming the 19 common *** content of the 10 components was basically *** components’transfer rates were within 30%of their respective ***:This study provided a comprehensive and reliable strategy for the quality evaluation of Sanhua decoction substance benchmarks and held significant importance in improving its application value.

关键词： Sanhua decoction classic famous formula HPLC fingerprinting value transfer network pharmacology

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